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• Isolated mitochondria
  ...ents to the cytoskeleton, followed by centrifugation steps to separate the mitochondria from other components. == Isolated mitochondria versus permeabilized cells ==
  899 bytes (101 words) - 11:02, 10 April 2022
• Mitochondria, isolated
  #REDIRECT [[Isolated mitochondria]]
  35 bytes (3 words) - 16:26, 14 September 2010
• O2k-Publications: Isolated Mitochondria
  ...lications]] [[Instrument and method::Oxygraph-2k]] [[Preparation::Isolated mitochondria]] == O2k-Publications: Isolated mitochondria - Abstract==
  1 KB (172 words) - 11:37, 11 November 2016
• RCR is low in isolated mitochondria
  51 bytes (5 words) - 14:51, 15 June 2015

Page text matches

• Isolated mitochondria
  ...ents to the cytoskeleton, followed by centrifugation steps to separate the mitochondria from other components. == Isolated mitochondria versus permeabilized cells ==
  899 bytes (101 words) - 11:02, 10 April 2022
• Haller 1990 CYCYCLOBIOS Mitos
  ...(1990) Methodological considerations for respiratory studies with isolated mitochondria. CYCLOBIOS Newsletter 4:51-5. |preparations=Isolated mitochondria
  444 bytes (46 words) - 12:36, 24 February 2015
• MiPNet11.05 imt-pti-pce
  ...k=http://wiki.oroboros.at/index.php/O2k-Protocols|O2k-Protocols]] Isolated mitochondria or permeabilized tissues and cells. |abstract=Gnaiger E (2022) Isolated mitochondria or permeabilized tissues and cells. Mitochondr Physiol Network 11.05(07):1-
  943 bytes (113 words) - 14:17, 10 April 2022
• McKee E
  |area_of_interest=Isolated mitochondria |area_of_interest=Isolated mitochondria
  468 bytes (57 words) - 17:10, 12 February 2018
• Raaflaub 1953 Helv Physiol Pharmacol Acta
  |title=Raaflaub J (1953) Swelling of isolated mitochondria of the liver and their susceptibility to physicochemical influences. Helv P |preparations=Isolated mitochondria
  463 bytes (55 words) - 10:10, 26 February 2015
• Ernster 1962 Nature
  ...succinate-linked acetoacetate reduction in tissue homogenates and isolated mitochondria. Nature 193:1050-2. |preparations=Homogenate, Isolated mitochondria
  508 bytes (56 words) - 11:14, 28 May 2015
• Raich 1990 CYCLOBIOS
  ...tochondria isolated from oligochaetes: Comparison with classical rat liver mitochondria. CYCLOBIOS Newsletter 4:77-9. |preparations=Isolated mitochondria
  636 bytes (71 words) - 04:49, 23 November 2021
• Holian 1977 Arch Biochem Biophys
  |title=Holian A, Owen CS, Wilson DF (1977) Control of Respiration in Isolated Mitochondria: Quantitative Evaluation of the Dependence of Respiratory Rates on [ATP], [ |preparations=Isolated mitochondria
  440 bytes (58 words) - 12:45, 24 February 2015
• Strobel A
  |area_of_interest=Isolated mitochondria, Permeabilized cells, Permeabilized muscle fibers {{Labelingperson|area_of_interest=Isolated mitochondria, Permeabilized cells, Permeabilized muscle fibers
  564 bytes (63 words) - 17:11, 12 February 2018
• Willet 2000 Biochim Biophys Acta
  |title=Willet K, Detry O, Sluse FE (2000) Resistance of isolated pulmonary mitochondria during in vitro anoxia/reoxygenation. Biochim Biophys Acta 1460:346-52. ...'' anoxia/reoxygenation (up to 45 min anoxia). These results indicate that mitochondria are not directly responsible of their oxidative phosphorylation damage obse
  2 KB (214 words) - 11:43, 16 June 2015
• Doerrier 2022 MitoFit NADH
  ...oxygen consumption and NAD(P)H/NAD(P)⁺-redox state in isolated mitochondria. (in prep) ...tate, high-resolution respirometry HRR, isolated mitochondria, mouse liver mitochondria, SUIT protocols, N-pathway
  946 bytes (114 words) - 10:06, 3 May 2022
• Schoettl 2015 Fatty Acid Oxidation O2k-Network Discussion Forum
  ...act=Beta oxidation in adipocytes (white and brown), HEK293 cells, isolated mitochondria from liver, adipocytes, brain, etc. |preparations=Isolated mitochondria
  532 bytes (68 words) - 15:55, 9 November 2016
• Pasdois 2015 Fatty Acid Oxidation O2k-Network Discussion Forum
  ...''Protocol''': Couple palmitoylcarnitine (10µM) + malate (1mM) on isolated mitochondria and permeabilized fibers. In such case the buffer is always supplemented wi |preparations=Permeabilized tissue, Isolated mitochondria
  582 bytes (74 words) - 11:27, 8 November 2016
• Hanackova L
  |area_of_interest=Isolated mitochondria, Cultured cells, Intact cells, Permeabilized cells |area_of_interest=Isolated mitochondria, Cultured cells, Intact cells, Permeabilized cells
  518 bytes (61 words) - 17:10, 12 February 2018
• MiPNet12.11 MitoRespiration
  ...ht|80px|link=O2k-Protocols|O2k-Protocols]] Flux control ratios in isolated mitochondria. OXPHOS capacity and respiratory control. ...isolatd mitochondria. OXPHOS capacity and respiratory control in isolated mitochondria. Mitochondr Physiol Network 12.11(06):1-5.
  1 KB (149 words) - 12:24, 4 December 2019
• Euthanyl/Pentobarbitol
  ...mi K, Suga H (1997) No suppression of respiratory function of mitochondria isolated from the hearts of anesthetized rats with high-dose pentobarbital sodium. J |preparations=Isolated Mitochondria
  590 bytes (77 words) - 13:42, 15 June 2015
• Dickinson 1967 Science
  ...DB, Misch MJ, Drury RE (1967) Dimethyl sulfoxide protects tightly coupled mitochondria from freezing damage. Science 156:1738-9. ...were frozen in liquid nitrogen without dimethyl sulfoxide. Thus, isolated mitochondria provide a model system for the study of the effects of freezing on biologic
  1 KB (144 words) - 10:14, 9 November 2016
• MitoPedia: Sample preparations
  :» [[O2k-Publications: Isolated Mitochondria |Isolated mitochondria]]
  504 bytes (49 words) - 08:34, 24 January 2016
• Siekevitz 1956 J Biophys Biochem Cytol-I
  ...I. The separation and identification of a membrane fraction from isolated mitochondria. J Biophys Biochem Cytol 2:639-51. ...tochondria do possess membranes as defined and that these membranes can be isolated in a relatively pure form.
  1 KB (207 words) - 10:33, 28 May 2015
• Felser Andrea
  |area_of_interest=Isolated mitochondria, Intact cells, Permeabilized cells {{Labelingperson|area_of_interest=Isolated mitochondria, Intact cells, Permeabilized cells
  706 bytes (82 words) - 14:59, 11 December 2021
• Sivitz 2017 Neuromethods
  |title=Sivitz WI (2017) Techniques to investigate bioenergetics of mitochondria. In: Strack S, Usachev Y (eds) Techniques to investigate mitochondrial func ...one is assessing isolated mitochondria or intact cells. Although isolated mitochondria remain the only way to evaluate properties intrinsic to the organelles per
  1 KB (171 words) - 05:30, 23 October 2023
• Mark Felix Christopher
  |area_of_interest=Isolated mitochondria, Permeabilized cells, Permeabilized muscle fibers |area_of_interest=Isolated mitochondria, Permeabilized cells, Permeabilized muscle fibers
  664 bytes (82 words) - 15:36, 5 August 2020
• Lehr 2021 Methods Mol Biol
  |title=Lehr S, Hartwig S, Kotzka J (2021) Preparation of "functional" mitochondria: a challenging business. https://doi.org/10.1007/978-1-0716-1266-8_2 ...adient) centrifugation and depict major challenges to achieve "functional" mitochondria as basis for comprehensive physiological studies.
  1 KB (193 words) - 17:28, 24 April 2023
• Valenti 2014 Anal Biochem
  ...(2014) Preservation of mitochondrial functional integrity in mitochondria isolated from small cryopreserved mouse brain areas. Anal Biochem 444:25-31. ...mall brain areas from which intact and phosphorylating mitochondria can be isolated for the study of mitochondrial bioenergetics will significantly expand the
  2 KB (283 words) - 16:22, 13 November 2017
• O2k-Publications: Isolated Mitochondria
  ...lications]] [[Instrument and method::Oxygraph-2k]] [[Preparation::Isolated mitochondria]] == O2k-Publications: Isolated mitochondria - Abstract==
  1 KB (172 words) - 11:37, 11 November 2016
• Pecinova 2011 Mitochondrion
  ...he complex OXPHOS analysis, i.e. five times less as compared with isolated mitochondria. |keywords=Isolated mitochondria, Tissue homogenates, oxidative phosphorylation, Respiratory control, Mitoch
  2 KB (212 words) - 15:35, 13 November 2017
• Brewer 2004 Mitochondrion
  ...respiratory rates and improved creatine stimulation in brain mitochondria isolated with anti-oxidants. Mitochondrion 4:49-57. ...ed a two-fold higher Vmax and a 15% higher respiratory control ratio when mitochondria were prepared with SCAVEGR. SCAVEGR also stabilized the octameric state of
  1 KB (195 words) - 16:55, 16 January 2021
• Picard 2011 Aging Cell
  ...Mitochondrial functional impairment with aging is exaggerated in isolated mitochondria compared to permeabilized myofibers. Aging Cell 9:1032–46. ...mitochondrial function in sarcopenic muscle than suggested previously from isolated organelle studies.
  2 KB (302 words) - 12:10, 28 March 2018
• Pfleger 2023 Methods Mol Biol
  ...rial respiration in intact cells, permeabilized cells, and isolated tissue mitochondria using the Seahorse XF Analyzer. Methods Mol Biol 2022;2497:185-206. doi: 10 ...P production in intact and permeabilized cells, as well as in mitochondria isolated from tissue using the Seahorse XF Extracellular Flux Analyzer (Agilent Tech
  1 KB (178 words) - 12:49, 27 April 2023
• Rekuviene 2017 Data Brief
  ...d activities of respiratory chain complexes I and II in isolated rat brain mitochondria. Data Brief 13:707-12. ...mplexes and H₂O₂ generation in cortex and cerebellum mitochondria are described.
  2 KB (232 words) - 11:47, 11 June 2018
• Hosler 2023 Mitochondrion
  ...rds K (2023) The cyclic lipopeptide micafungin induces rupture of isolated mitochondria by reprograming the mitochondrial inner membrane anion channel. https://doi ...ungin initiates the uptake of salts, causing rapid swelling and rupture of mitochondria with release of cytochrome c. The inner membrane anion channel (IMAC) is al
  2 KB (204 words) - 15:38, 19 December 2023
• Lee 1974 Anal Biochem
  |title=Lee NH, Shapiro IM (1974) A method for isolating respiring mitochondria from epiphyseal cartilage. Anal Biochem 58:117-22. |preparations=Isolated mitochondria
  344 bytes (42 words) - 16:50, 24 February 2015
• Weinbach 1961 J Biol Chem
  ...Weinbach EC (1961) Oxidative phosphorylation with endogenous substrates of mitochondria . J Biol Chem 236:1526-30. |preparations=Isolated mitochondria
  396 bytes (47 words) - 13:32, 14 February 2022
• Rousar Tomas
  |area_of_interest=Isolated mitochondria, Cultured cells, Intact cells, Permeabilized cells |area_of_interest=Isolated mitochondria, Cultured cells, Intact cells, Permeabilized cells
  798 bytes (98 words) - 15:41, 14 April 2020
• Hiltunen 1981 Biochim Biophys Acta
  ...) The disposition of citric acid cycle intermediates by isolated rat heart mitochondria. Biochim Biophys Acta 678:115-21. ...are involved in conversion of propionate to pyruvate in isolated rat heart mitochondria.
  2 KB (209 words) - 17:26, 7 November 2016
• Palmer 1977 J Biol Chem
  ...) Biochemical properties of subsarcolemmal and interfibrillar mitochondria isolated from rat cardiac muscle. J Biol Chem 252:8731-9. ...bstrates tested approximately 1.5 times faster than did the subsarcolemmal mitochondria. Thus the two mitochondrial types differed not only in their respective loc
  2 KB (235 words) - 08:48, 16 July 2021
• Long 2019 Methods Mol Biol
  ..., Huang K, Yang Q (2019) Assessing mitochondrial bioenergetics in isolated mitochondria from mouse heart tissues using Oroboros Oxygraph-2k. Methods Mol Biol 1966: ...al subpopulations, subsarcolemmal mitochondria (SSM) and intermyofibrillar mitochondria (IFM). These approaches can easily be translated to other cells and tissues
  1 KB (191 words) - 18:21, 29 February 2020
• Lee 1974 Calcif Tissue Res
  |title=Lee NH, Shapiro IM (1974) Oxidative phosphorylation by chondrocyte mitochondria. Calcif Tissue Res 16:277-82. |preparations=Isolated mitochondria
  338 bytes (40 words) - 16:50, 24 February 2015
• Appaix 2000 Biochim Biophys Acta
  ...ophotometric method for quantitation of cytochrome c release from isolated mitochondria or permeabilized cells revisited. Biochim Biophys Acta 1457:175-81. ...oxygraphic determination of cytochrome c-dependent respiration of isolated mitochondria and permeabilized cardiomyocytes.
  1 KB (194 words) - 16:48, 9 November 2017
• Skorkowski 1984 Comp Biochem Physiol
  ...for the role of malic enzyme in the rapid oxidation of malate by cod heart mitochondria. Comp Biochem Physiol B 77:379-84. ...he rapid oxidation of malate (as the only external substrate) by cod heart mitochondria.
  2 KB (312 words) - 09:14, 9 November 2016
• McCann 2012 J Neurochem
  ...f deoxypyrimidines and deoxypyrimidine antiviral analogs in isolated brain mitochondria. J Neurochem 122:126-37. ...dC, and AZT were significantly different in brain mitochondria compared to mitochondria from liver and heart.
  2 KB (287 words) - 17:12, 12 October 2021
• Wu 2018 Adv Sci
  ...upte A, Blanco E, Hamilton DJ (2018) Polymer functionalization of isolated mitochondria for cellular transplantation and metabolic phenotype alteration. Adv Sci 17 ...represent the first demonstration of polymer functionalization of isolated mitochondria, highlighting a viable strategy for enabling clinical applications of mitoc
  2 KB (258 words) - 12:01, 26 February 2018
• Kennedy 1949 J Biol Chem
  ...tty acids and tricarboxylic acid cycle intermediates by isolated rat liver mitochondria. J Biol Chem 179:957-72. ...ons of mitochondria. These bodies have insignificant glycolytic activity. "Mitochondria" prepared by other methods involving saline or water as the dispersing medi
  1 KB (163 words) - 12:58, 13 February 2015
• Barksdale 2010 FASEB J
  ...olic processes in health and disease. It is also possible to archive these mitochondria for future studies. |preparations=Isolated mitochondria
  2 KB (276 words) - 15:50, 15 June 2015
• Reynolds 2004 Physiol Biochem Zool
  |title=Reynolds JA, Hand SC (2004) Differences in isolated mitochondria are insufficient to account for respiratory depression during diapause in ' ...uring diapause cannot be fully explained by altered properties of isolated mitochondria. Rather, mechanisms for active inhibition or substrate limitation of mitoch
  2 KB (314 words) - 09:45, 9 November 2016
• Crane 1957
  ...atefi Y, Lester RL, Widmer C (1957) Isolation of a quinone from beef heart mitochondria. Biochim Biophys Acta 25:220-1. |keywords=Beef heart mitochondria, quinone
  484 bytes (64 words) - 11:20, 24 February 2015
• Sobotka 2015 Physiol Res
  ...The effect of α-tocopheryl succinate on succinate respiration in rat liver mitochondria. Physiol Res 64 Suppl 5:S609-15. ...ibitory effect was tested, the most sensitive in both states were isolated mitochondria; the most resistant were permeabilized hepatocytes.
  2 KB (208 words) - 16:09, 13 November 2017
• Hadsell 2009 J Dairy Sci
  ...009) ''Technical note:'' assessing the functional capacity of mitochondria isolated from lactating mammary tissue: choose your chelating agent wisely. J Dairy ...l facilitate future studies aimed at determining the importance of mammary mitochondria to milk production.
  2 KB (326 words) - 14:21, 20 May 2015
• Dlaskova 2010 Biochim Biophys Acta
  ...The role of UCP 1 in production of reactive oxygen species by mitochondria isolated from brown adipose tissue. Biochim Biophys Acta 1797:1470-6. ...ein 1 can affect reactive oxygen containing species production by isolated mitochondria from brown adipose tissue.
  2 KB (239 words) - 12:09, 26 May 2015
• Scheibye-Knudsen 2009 Eur J Appl Physiol
  ...by inorganic phosphate: comparing permeabilized muscle fibers and isolated mitochondria prepared from type-1 and type-2 rat skeletal muscle. Eur J Appl Physiol 105
  2 KB (243 words) - 11:31, 15 June 2015
• Schwerzmann 1989 Proc Natl Acad Sci U S A
  ...K, Hoppeler H, Kayar SR, Weibel ER (1989) Oxidative capacity of muscle and mitochondria: correlation of physiological, biochemical, and morphometric characteristic ...f isolated mitochondria was very near to the maximal oxygen uptake rate of mitochondria that is commonly estimated in intact muscles of a wide variety of animals.
  2 KB (289 words) - 18:00, 16 January 2021
• Chen 2016 Nat Chem Biol
  ...chaefer J, Patti GJ (2016) Lactate metabolism is associated with mammalian mitochondria. Nat Chem Biol 12:937-43. ...ogether, our results demonstrate a link between lactate metabolism and the mitochondria of fermenting mammalian cells.
  2 KB (253 words) - 17:19, 9 November 2017
• Claude 1945 J Exp Med
  |title=Claude A, Fullam EF (1945) An electron microscope study of isolated mitochondria: method and preliminary results. J Exp Med 81:51-62. ...=1. The present paper constitutes a preliminary study of the morphology of mitochondria by means of electron microscopy.
  2 KB (265 words) - 11:13, 24 February 2015
• Gray 2017 Neuromethods
  ...; (2) by mitochondria isolated from HEK293T cells, and (3) by mitochondria isolated from mouse liver, which are useful as a general model of mitochondrial func |keywords=Mitochondria, Pyruvate, Oxidation, Respiration, Metabolism
  2 KB (240 words) - 13:34, 14 June 2017
• Kelly 2011 Mitochondrion
  ...ated and activated uncoupling protein 3 (UCP3) in isolated skeletal muscle mitochondria following administration of 3,4-methylenedioxymethamphetamine (MDMA aka ecs ...de that MDMA treatment of animals increases proton leak in skeletal muscle mitochondria by activating UCP3 through ''in vivo'' covalent modification of UCP3 by pho
  2 KB (337 words) - 14:53, 13 November 2017
• Mould 2023 Front Physiol
  ...homas EL, Guy GW, Nunn AVW, Bell JD (2023) Non-chemical signalling between mitochondria. Front Physiol 14:1268075. https://doi.org/10.3389/fphys.2023.1268075 ...results support the existence of non-chemical signalling between isolated mitochondria. The experimental design suggests that the non-chemical communication is li
  2 KB (244 words) - 23:37, 7 February 2024
• Bensley 1934 Anat Rec
  ...ucture by the freezing-drying method VI. The preparation and properties of mitochondria. Anat Rec 60:449-55. ::::* [[Mitochondria and bioblasts: Made history]]
  577 bytes (68 words) - 10:41, 6 July 2022
• Ziak 2015 Mitochondrion
  ...Jiroutkova K, Nemcova V, Dzupa V, Duska F (2015) Assessing the function of mitochondria in cytosolic context in human skeletal muscle: Adopting high-resolution res ...n flux). Homogenate retained ~ 90% of phosphorylation capacity of isolated mitochondria. The use of fresh homogenate was crucial as mitochondrial function declined
  1 KB (200 words) - 14:37, 26 March 2018
• Mitochondrial preparations
  |description='''Mitochondrial preparations''' (mtprep) are isolated mitochondria (imt), tissue homogenate (thom), mechanically or chemically permeabilized t ::::* [[Isolated mitochondria]], imt
  1 KB (166 words) - 14:29, 11 December 2022
• El-Mir 2000 J Biol Chem
  .... Studies using functionally isolated complex I obtained from mitochondria isolated from dimethylbiguanide-pretreated livers or rats further confirmed that dim |preparations=Permeabilized cells, Isolated mitochondria, Intact cells
  2 KB (298 words) - 16:25, 20 November 2023
• Toleikis 2005 Biosci Rep
  ...umbeckaite S, Majiene D (2005) Cytochrome C effect on respiration of heart mitochondria: influence of various factors. Biosci Rep 25:387-97. ...rate. Similar results were obtained with the isolated rat and rabbit heart mitochondria. The differences in the degree of stimulation of mitochondrial respiration
  2 KB (233 words) - 16:18, 13 November 2017
• Herbst 2014 Abstract MiP2014
  ...ructure of the mitochondria and also yields a final suspension composed of mitochondria from multiple sub-populations from various cell types, making physiological ...ronic perturbations and compared our results with the traditional isolated mitochondria preparation.
  2 KB (327 words) - 15:22, 20 October 2017
• Fisar 2016a Folia Biol (Praha)
  |title=Fisar Z, Hroudova J (2016) Pig brain mitochondria as a biological model for study of mitochondrial respiration. Folia Biol (P ...y animals. We suggest the use of high-resolution respirometry of pig brain mitochondria for research of the neuroprotective effects and/or mitochondrial toxicity o
  2 KB (321 words) - 17:57, 9 November 2017
• Claude 1940 Science
  ::::* [[Mitochondria and bioblasts: Made history]] |preparations=Homogenate, Isolated mitochondria
  494 bytes (57 words) - 10:41, 6 July 2022
• Kanner 1976 Biochem Biophys Res Commun
  ...on and characterization of homogeneous coupling factor 6 from bovine heart mitochondria. Biochem Biophys Res Commun 69:1050-6. ...were isolated from the rutamycin-sensitive ATPase complex of bovine heart mitochondria by heating and fractionation with ethanol. F6 appeared in acrylamide gel el
  1 KB (150 words) - 13:03, 24 February 2015
• Cannon 2008 Methods Mol Biol
  ...tential influence on whole body metabolism, and specific properties of the mitochondria and their mode of function may be addressed using these methods. |keywords=Adipose tissue, Heat production, Mitochondria
  1 KB (194 words) - 17:10, 9 November 2017
• Nukala 2006 J Neurosci Methods
  ...ukala VN, Singh IN, Davis LM, Sullivan PG (2006) Cryopreservation of brain mitochondria: A novel methodology for functional studies. J Neurosci Methods 152:48-54. ...yopreserved brain mitochondria observed allows us the opportunity to store mitochondria for longer periods of time as well as perform metabolic studies as needed.
  2 KB (282 words) - 15:30, 13 November 2017
• Yamaguchi 2007 Cell Death Differ
  ...uchi R, Andreyev A, Murphy AN, Perkins GA, Ellisman MH, Newmeyer DD (2007) Mitochondria frozen with trehalose retain a number of biological functions and preserve ...trehalose-frozen mitochondria retained most of the biological features of mitochondria including mtOM integrity. Although not ideal for studies involving bioenerg
  2 KB (227 words) - 17:03, 19 October 2017
• Rogers 2011 PLoS One
  ...t microplate respiratory measurements using minimal quantities of isolated mitochondria. PLoS One 6:e21746. ...r that inclusion of phosphatase inhibitors in the preparation of the heart mitochondria results in a specific decrease in rates of Complex I-dependent respiration.
  2 KB (344 words) - 10:48, 26 February 2015
• Kuznetsov 1998 BTK
  ...muscle fibers, collagenasetreated permeabilized muscle fibers and isolated mitochondria. |preparations=Permeabilized tissue, Isolated mitochondria
  2 KB (222 words) - 04:27, 23 November 2021
• MiPNet20.15 IsolationRatHeart-mt
  ...=O2k-Protocols|O2k-Protocols]] Laboratory protocol: isolation of rat heart mitochondria. ...ntana M, Krumschnabel G (2016) Laboratory protocol: isolation of rat heart mitochondria. Mitochondr Physiol Network 20.15(02):1-2.
  826 bytes (103 words) - 17:08, 13 December 2019
• Moyes 1988 Am J Physiol
  ...CD, Buck LT, Hochachka PW (1988) Temperature effects on pH of mitochondria isolated from carp red muscle. Am J Physiol 254:R611-5. ...0.4 U at 10, 20, and 30 degrees C. These data suggest that carp red muscle mitochondria defend an appropriate mitochondrial pH gradient with temperature-induced ch
  2 KB (258 words) - 09:11, 9 November 2016
• Clarke 2018 Methods Mol Biol
  ...tivity when measuring uncoupling protein 1 (UCP1) function in mitochondria isolated from brown adipose tissue. Methods Mol Biol 1782:325-36. ...UCP1 activity in the presence of calcium for isolated brown adipose tissue mitochondria.
  2 KB (287 words) - 09:05, 27 July 2018
• Schwartz 1962 Circ Res
  |title=Schwartz A, Lee KS (1962) Study of heart mitochondria and glycolytic metabolism in experimentally induced cardiac failure. Circ R |preparations=Isolated mitochondria
  3 KB (377 words) - 11:26, 28 May 2015
• Cortassa 2015 Fatty Acid Oxidation O2k-Network Discussion Forum
  |abstract=Palmitoyl CoA (mitochondria) and Palmitate (conjugated with BSA) in cardiomyocytes. |preparations=Intact cells, Isolated mitochondria
  500 bytes (63 words) - 11:03, 8 November 2016
• Sivertsson 2013 Adv Exp Med Biol
  ...in diabetes. The functional significance of the mTOR pathway in regulating mitochondria efficiency warrants further attention. |preparations=Isolated mitochondria
  2 KB (269 words) - 17:30, 23 February 2020
• Joergensen 2015 J Cereb Blood Flow Metab
  ...N, Quistorff B (2015) One-year high fat diet affects muscle-but not brain mitochondria. J Cereb Blood Flow Metab 35:943-50. ...od-brain barrier may play a role in this strikingly different phenotype of mitochondria from the two tissues of the same animal.
  2 KB (302 words) - 17:42, 7 November 2016
• Nie 2021 Sci Adv
  ...agl R (2021) Quantum monitoring of cellular metabolic activities in single mitochondria. Sci Adv 7:eabf0573. https://doi.org/10.1126/sciadv.abf0573 ...ls generated by individual mitochondria in either living cells or isolated mitochondria after stimulation or inhibition.
  2 KB (243 words) - 05:05, 6 February 2024
• Chweih 2015 Exp Physiol
  ...eira TR (2015) Tissue and sex specificities in Ca(2+) handling by isolated mitochondria in conditions avoiding the permeability transition. Exp Physiol 100(9):1073 ...rties and the physiological variability of the Ca(2+) handling by isolated mitochondria.
  3 KB (433 words) - 16:03, 7 November 2016
• Combs 2004 Methods Enzymol
  ...tochondria. The NADH ED-FRAP measurement of kinetic parameters in isolated mitochondria is also elaborated.
  1 KB (210 words) - 16:53, 8 February 2022
• Palmeira 2012 Methods Mol Biol
  ...mbrane potential (ΔΨ) fluctuations associated with the metabolic states of mitochondria. Methods Mol Biol 810:89-101. ...ive electrode. The main focus is given to the evaluation of ΔΨ in isolated mitochondria.
  1 KB (182 words) - 12:55, 3 April 2021
• MiPNet20.08 IsolationRatLiver-mt
  .../O2k-Protocols|O2k-Protocols]] Laboratory Protocol: isolation of rat liver mitochondria. ...tract=Sumbalova Z, Fontana M, Krumschnabel G (2016) Isolation of rat liver mitochondria. Mitochondr Physiol Network 20.08(02):1-2.
  912 bytes (116 words) - 17:10, 13 December 2019
• Martin 1999 PBZ
  ...nd SC (1999) Reversible depression of oxygen consumption in isolated liver mitochondria during hibernation. Physiol. Biochem. Zool. 72:255-64. ...findings indicate that a depression of ADP-dependent respiration in liver mitochondria occurs during torpor and is reversed during the interbout arousals to euthe
  2 KB (265 words) - 14:30, 28 March 2018
• Schoen 1990 Cyclobios
  ...hön A, Haller T, Gnaiger E (1990) Hexokinase induced, steady state coupled mitochondria studied by injection calorimetry and respirometry. CYCLOBIOS Newsletter 4:6 |preparations=Isolated mitochondria
  554 bytes (67 words) - 04:48, 23 November 2021
• Phung 2011 J Food Science
  ...linde E (2011) Oxygen consumption rate of permeabilized cells and isolated mitochondria from pork M. masseter and liver examined fresh and after freeze-thawing at ...wing. Low pH was associated with reduced OCRS. The OCRS of isolated muscle mitochondria appeared to be an underestimated relative to the OCRS of permeabilized musc
  2 KB (318 words) - 10:09, 26 February 2015
• MiPNet08.13 mt-Isolation-RLM
  .../O2k-Protocols|O2k-Protocols]] Laboratory protocol: Isolation of rat liver mitochondria. ...tract='''Lassnig B, Gnaiger E. Laboratory protocol: Isolation of rat liver mitochondria. Mitochondr Physiol Network 08.13.'''
  1 KB (127 words) - 10:41, 10 April 2022
• Hassoun 2006 Mitochondrion
  ...nderstood. Here, we studied the effects of sphingosine on isolated cardiac mitochondria of either wild-type or Bcl-2 overexpressing transgenic mice. Sphingosine in |keywords=Sphingosine, Heart, Mitochondria, Cyclosporine, Bcl-2
  1 KB (184 words) - 11:50, 22 December 2020
• MiPNet20.09 IsolationBeefHeart-mt
  ...O2k-Protocols|O2k-Protocols]] Laboratory protocol: isolation of beef heart mitochondria. ...ontana M, Gomez Rodriguez A, Krumschnabel G (2015) Isolation of beef heart mitochondria. Mitochondr Physiol Network 20.09(01):1-2.
  946 bytes (119 words) - 17:09, 13 December 2019
• Elustondo 2013 J Biol Chem
  ...functional association of lactate dehydrogenase (LDH) with skeletal muscle mitochondria. J Biol Chem 288:25309-17. ...lly positioned within skeletal muscle fibers to functionally interact with mitochondria.
  2 KB (267 words) - 13:34, 27 April 2015
• Tretter 2007 J Neurochem
  ...ffect on the production of reactive oxygen species by ''in situ'' synaptic mitochondria. J Neurochem 103:1864-71. ...generation is limited mainly to well-coupled succinate-supported isolated mitochondria.
  3 KB (365 words) - 16:51, 5 April 2022
• Affourtit 2012 Methods Mol Biol
  ..., Brand MD (2012) Measurement of proton leak and electron leak in isolated mitochondria. Methods Mol Biol 810:165-82. ...s are presented to measure proton and electron leak activities in isolated mitochondria. The relative strength of a modular kinetic approach to probe oxidative pho
  1 KB (154 words) - 13:04, 7 March 2020
• Vinnakota 2016b Biophys J
  ...n-loop control of oxidative phosphorylation in skeletal and cardiac muscle mitochondria by Ca²⁺. Biophys J 110:954-61. ...imulates NADH synthesis in skeletal muscle mitochondria but not in cardiac mitochondria.
  2 KB (300 words) - 17:11, 7 November 2016
• Djafarzadeh 2017b J Vis Exp
  |title=Djafarzadeh S, Jakob SM (2017) Isolation of intact mitochondria from skeletal muscle by differential centrifugation for High-Resolution Res ...isolated mitochondria, based on differential centrifugation, are that the mitochondria can be damaged during the homogenization and isolation procedure and that l
  2 KB (255 words) - 17:53, 22 November 2017
• Lee 1964 Nature
  ...upling in non-phosphorylating electron transport particles from beef-heart mitochondria. Nature 201:152-5. |preparations=Isolated mitochondria
  565 bytes (70 words) - 12:02, 28 May 2015
• O´Gorman 1997 FEBS Lett
  ...ibited the latter only in transgenic liver mitochondria. Protein complexes isolated from detergent solubilised rat brain extracts, containing octameric mitocho ...denine nucleotide translocator, Apoptosis, Creatine kinase, Cyclocreatine, Mitochondria, Permeability transition
  2 KB (220 words) - 15:37, 15 June 2015
• McMillin-Wood 1980 Biochim Biophys Acta
  ...e CA, Goldstein MA, Entman ML (1980) Calcium uptake by two preparations of mitochondria from heart. Biochim Biophys Acta 591:251-65. ...erence to their presumed intracellular location may pertain to the role of mitochondria as an intracellular Ca2+ buffering mechanism in contractile tissue.
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• Ocloo 2017 J Appl Pharmac Sc
  ...espirations supported by all the substrates in both the isolated rat liver mitochondria and the permeabilised cardiac tissues. Thus, whilst O, O-dimethyl-alpinumis |preparations=Isolated mitochondria
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• Clarke 2013 Int J Biochem Cell Biol
  ...ncoupling protein 1 dependent reactive oxygen species production by thymus mitochondria. Int J Biochem Cell Biol 45:81-9. ...ondria. We also show that reverse electron transport is possible in thymus mitochondria respiring on succinate and glycerol-3-phosphate. The implications of this r
  2 KB (237 words) - 13:51, 27 March 2018
• Scaduto 1999 Biophys J
  ...of DeltaPsi of isolated mitochondria. We also applied this approach to the isolated perfused heart to determine whether DeltaPsi could be monitored in an intac |preparations=Isolated mitochondria
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• Moore 1988 FEBS Letters
  ...h TJ (1988) Measurement of the redox state of the ubiquinone pool in plant mitochondria. FEBS Lett 235:76-80. ...ate on the redox poise of the quinone pool in isolated turnip and pea leaf mitochondria. A linear relationship has been found between these two parameters during s
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• Wu 2007 J Biol Chem
  ...hondrial membrane potential and a corresponding drop in the ability of the mitochondria to synthesize ATP at the hydrolysis potential required for cellular functio
  2 KB (260 words) - 14:55, 10 November 2021
• PGMSGp pathway control state
  ::::* DL-Protocol for isolated mitochondria and tissue homogenate (mt): [[SUIT-038_O2_mt_D091]] ::::* DL-Protocol for isolated mitochondria and tissue homogenate (mt): [[SUIT-038_O2_mt_D091]]
  1 KB (131 words) - 14:12, 12 April 2023
• Timohhina 2009 J Bioenerg Biomembr
  ...ambre T, Sackett D, Saks V (2009) Direct measurement of energy fluxes from mitochondria into cytoplasm in permeabilized cardiac cells ''in situ'': some evidence fo ...directly that under physiological conditions the major energy carrier from mitochondria into cytoplasm is PCr, produced by mitochondrial creatine kinase (MtCK), wh
  2 KB (266 words) - 11:08, 26 May 2015
• PalPGMSGp pathway control state
  ::::* DL-Protocol for isolated mitochondria and tissue homogenate (mt): [[SUIT-036_O2_mt_D089]] ::::* DL-Protocol for isolated mitochondria and tissue homogenate (mt): [[SUIT-036_O2_mt_D089]]
  1 KB (129 words) - 14:11, 12 April 2023
• Solinas 2014 Mech Ageing Dev
  ...(2014) Aging effects on oxidative phosphorylation in rat adrenocortical 3 mitochondria. Mech Ageing Dev 138:10-4. ...xidative phosphorylation. We conclude that aging results in adrenocortical mitochondria effects that are non-uniform across different rat strains.
  2 KB (233 words) - 16:10, 13 November 2017
• Hyperoxia
  ...ular and intracellular hyperoxia is imposed on isolated cells and isolated mitochondria at air-level oxygen pressures which are higher compared to cellular and int
  738 bytes (92 words) - 14:47, 21 October 2021
• Makrecka 2015 Fatty Acid Oxidation O2k-Network Discussion Forum
  ...in permeabilized muscle fibres and cardiac fibres and in isolated cardiac mitochondria of rats and mice. Since the area of our research is the regulation of energ |preparations=Permeabilized tissue, Isolated mitochondria
  1,013 bytes (143 words) - 14:24, 27 March 2018
• Hatefi 1961 Biochim Biophys Acta
  ...ighly active DPNH-cytochrome c reductase has been isolated from beef-heart mitochondria. The best preparations of the enzyme catalyze the reduction by DPNH of appr |keywords=DPNH-cytochrome c reductase, Beef heart mitochondria
  1 KB (154 words) - 14:33, 13 November 2017
• Haller 1990 CYCLOBIOS Kinetics
  ...ger E (1990) Kinetic control and reaction pressure in steady state coupled mitochondria. CYCLOBIOS Newsletter 4:63-6. |preparations=Isolated mitochondria
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• Costa 1997 Am J Physiol Cell Physiol
  ...r controls, indicating that the intrinsic properties and the efficiency of mitochondria do not change as a consequence of adaptation to hypoxia. |preparations=Isolated mitochondria
  2 KB (305 words) - 12:06, 8 November 2016
• Martinez 2018 Arch Biochem Biophys
  ...ondria. This work shows that wild type α-synuclein interacts with isolated mitochondria and translocates into the mitochondrial matrix. This interaction and the ir |keywords=Mitochondria, Mitochondrial metabolism, Parkinson's disease, α-synuclein
  2 KB (275 words) - 16:26, 11 July 2018
• Griffiths 1995 Biochem J
  ...rug to stabilize pore closure and so decrease the loss of [3H]DOG from the mitochondria during their preparation. |preparations=Isolated mitochondria
  3 KB (369 words) - 08:36, 16 June 2015
• Drahota 2005 Cell Biochem Biophys
  ...; activation by detergent was up to 20-fold in 1-d-old animals, whereas in mitochondria from 60-d-old rats it was only 7-fold. Our data indicate that the rat heart |keywords=Rat heart, Postnatal development, Cytochrome-c oxidase, Mitochondria, Detergent
  1 KB (195 words) - 11:09, 13 February 2015
• Razak 2018 RUMeS
  ...F, Ngah WZW, Makpol S, Damanhuri MHA, Karim NA, Tan JK (2018) Isolation of mitochondria and measurement of oxygen consumption rate by high-resolution respirometry |info=[http://rumesjournal.com/content/isolation-mitochondria-and-measurement-oxygen-consumption-rate-high-resolution-respirometry Open A
  2 KB (322 words) - 11:47, 8 April 2019
• Lanza 2011 J Magnetic Resonance Imaging
  ...ctroscopy with in vitro measurements of oxidative capacity in mitochondria isolated from muscle biopsies of the same muscle group in 18 healthy adults. ...mal isometric knee extension. State 3 respiration was measured in isolated mitochondria using high-resolution respirometry. A second cohort of 10 individuals under
  2 KB (223 words) - 13:12, 24 February 2015
• Takahashi 2017 Exp Gerontol
  ...enzyme Q-responsive regulation of respiratory complex IV activity in brain mitochondria. Exp Gerontol 98:217-223. ...e propose a novel function of OPA1 in regulating the CIV activity in brain mitochondria in response to CoQ₁₀.
  2 KB (304 words) - 17:37, 25 February 2020
• Kristian 2007 J Neurochem
  ...Fiskum G, Andrews SB (2007) Calcium-induced precipitate formation in brain mitochondria: composition, calcium capacity, and retention. J Neurochem 102(4):1346-56. ...ntion&expanded_search_query=Calcium-induced+precipitate+formation+in+brain+mitochondria%3A+composition%2C+calcium+capacity%2C+and+retention PMID: 17663756 Open Acc
  2 KB (320 words) - 09:26, 7 May 2020
• Hayward 2018 Thesis
  ...t system complexes in IBE and SA. Leak respiration was more elevated in SA mitochondria following anoxia than in either IBE or torpor. These findings suggest that |preparations=Isolated mitochondria
  2 KB (242 words) - 16:13, 4 February 2019
• Silva 2018 Methods Mol Biol
  ...PJ (2018) Evaluation of respiration with clark-type electrode in isolated mitochondria and permeabilized animal cells. Methods Mol Biol 1782:7-29. ...ocus our technical description in the measurement of oxygen consumption by isolated mitochondrial fractions and by permeabilized cells.
  2 KB (226 words) - 09:41, 30 July 2018
• Jeger 2015 BioMed Res Internat
  ...S at wide dose and time ranges on hepatocytes and isolated skeletal muscle mitochondria. Human hepatocellular carcinoma cells (HepG2) ...4 hours. Respiration rates of intact and permeabilized cells and isolated mitochondria were measured by
  2 KB (291 words) - 14:46, 13 November 2017
• Chinopoulos 2011 Methods Mol Biol
  ...Thomas B, Ten V, Starkov AA (2011) Isolation and functional assessment of mitochondria from small amounts of mouse brain tissue. Methods Mol Biol 793:311-24. ...ion of mitochondrial membrane potential (ΔΨm) in small amounts of isolated mitochondria, adapted to a plate reader format.
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• Kuznetsov 2000 Transplant Proc
  ...Brandacher G, Steurer W, Margreiter R, Gnaiger E (2000) Isolated rat heart mitochondria and whole heart as models for mitochondrial cold ischemia-reperfusion injur ...e heart remain one of the major problems in heart transplantation. Damaged mitochondria lead to heart injury by the diminished cellular energy status, oxidative st
  1 KB (169 words) - 14:16, 27 December 2021
• Claude 1944 J Exp Med
  |title=Claude A (1944) The constitution of mitochondria and microsomes, and the distribution of nucleic acid in the cytoplasm of a ...tion with formed elements of relatively large size, namely microsomes, and mitochondria.
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• Cervinkova 2007 Altern Lab Anim
  ...2007) Evaluation of mitochondrial function in isolated rat hepatocytes and mitochondria during oxidative stress. Altern Lab Anim 35:353-61. ...nduced oxidative stress on mitochondria. Swelling was observed in isolated mitochondria exposed to tBHP, which could be prevented by cyclosporin A, which is eviden
  3 KB (374 words) - 10:17, 24 March 2015
• Lanza 2009 Methods Enzymol
  |title=Lanza IR, Nair KS (2009) Functional assessment of isolated mitochondria ''in vitro''. Methods Enzymol 457:349-72. ...of mitochondrial proteins provide insightful mechanistic information about mitochondria in a variety of tissue types.
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• Scaduto 1999
  ...of DeltaPsi of isolated mitochondria. We also applied this approach to the isolated perfused heart to determine whether DeltaPsi could be monitored in an intac
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• Schoenfeld 2016 J Cereb Blood Flow Metab
  ...> release from brain mitochondria fueled with malate, in contrast to liver mitochondria (2.25-fold rise). This does obviously not support the possible occurrence o |keywords=Mitochondria, Energy metabolism, Inflammation, Lipids, Neuroprotection, Amplex red
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• Ter Veld 2005 FEBS J
  ...p>. For mutant cardiac mitochondria, only the inline image of mitochondria isolated from the mtM-CK^–/– phenotype was different (i.e. twofold hi |preparations=Isolated mitochondria
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• Wiedemann 1998 FEBS Lett
  ...l coefficients were detected only at oxygen concentrations close to the KM value of cytochrome c oxidase, with saponin-permeabilized fibers a significant in |preparations=Permeabilized tissue, Isolated mitochondria
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• Mendez 1994 BTK-191
  ...Gnaiger E (1994) How does oxygen pressure control oxygen flux in isolated mitochondria? A methodological approach by high-resolution respirometry and digital data |preparations=Isolated mitochondria
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• Charles 2020 Nanomedicine (Lond)
  |abstract=The mechanistic study of the drug carrier-target interactions of mitochondria-unique nanoparticles composed of polypeptide-peptide complexes (mPoP-NPs). ...of mPoP-NPs on dynamic structure and functional wellbeing of mitochondria. Mitochondria morphology, respiration, membrane potential, reactive oxygen species genera
  2 KB (236 words) - 15:48, 19 October 2021
• Garnol 2014 Physiol Res
  ...bination of HFD and peroxidative stress potentiates HFD damaging effect of mitochondria because both branches of the respiratory chain (NADH- and flavoprotein-depe |preparations=Intact cells, Permeabilized cells, Isolated mitochondria
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• Guerrieri 1975 FEBS Lett
  ...in phospholipid vesicles inlayed with purified complex III from beef heart mitochondria. FEBS Lett 54:339-42. |preparations=Isolated mitochondria
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• O'Donoghue 2009 Biochim Biophys Acta
  ...R, Clarke KJ, Porter RK (2009) Control of choline oxidation in rat kidney mitochondria. Biochim Biophys Acta 1787:1135-9. ...idney mitochondria. We conclude that the choline transporter in rat kidney mitochondria is the major site of control over the production of the organic osmolyte, b
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• Smith 2012 Biochem J
  ...ivity is inhibited by 33% in resting muscle compared with >95% in isolated mitochondria. Additionally, the ability of M-CoA to inhibit CPT-I appears to be dependen |preparations=Permeabilized tissue, Isolated mitochondria
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• Fisar 2017 Indian J Biochem Biophys
  ...experiments to test the activity of electron transfer system of pig brain mitochondria. Indian J Biochem Biophys 54:258-72. ...inants in purified mitochondria. High-resolution respirometry of pig brain mitochondria can be recommended as a suitable assay in research of neuroprotective effec
  2 KB (327 words) - 12:38, 29 May 2018
• Joergensen 2017 Physiol Rep
  ...gh-fat or a high-sucrose diet eliminates metabolic flexibility in isolated mitochondria from rat skeletal muscle. Physiol Rep 5. pii: e13207. ...here have major influence on key metabolic performance of skeletal muscle mitochondria.
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• Tuena 1969 Eur J Biochem
  ...Sandoval F (1969) Effect of ATP on the oxidation of succinate in rat brain mitochondria. Eur J Biochem 11:283-90. ...succinate and on the succinic dehydrogenase activity of isolated rat brain mitochondria has been studied.
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• Siewiera 2012 Int J Pharm
  ...vealed that PAMAM G3 could be successfully used in the protection of heart mitochondria against MG-induced impaired respiratory activity. Despite these promising d |keywords=Dendrimer PAMAM G3, heart mitochondria, mitochondrial bioenergetics, experimental diabetes, in vivo study
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• St-Pierre 2000 Am J Physiol Regul Integr Comp Physiol
  ...l GJ, Boutilier RG (2000) Metabolic depression and enhanced O2 affinity of mitochondria in hypoxic hypometabolism. Am J Physiol Regul Integr Comp Physiol 279:R1205 ...te 4 and state 3 rates and 2) an increase in the O₂ affinity of mitochondria. These findings demonstrate that metabolic depression can be reflected at a
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• Khattar 2016 J Neurosci Methods
  ...er RM (2016) Isolation of functionally active and highly purified neuronal mitochondria from human cortex. J Neurosci Methods 263:1-6. ...tissue and cell dependent, but isolation of highly purified human neuronal mitochondria is not currently available.
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• Manzke L
  |area of interest=Isolated mitochondria
  362 bytes (45 words) - 17:10, 12 February 2018
• Hulbert 2006 J Comp Physiol B
  ...ert AJ, Turner N, Hinde J, Else P, Guderley H (2006) How might you compare mitochondria from different tissues and different species?. J Comp Physiol B 176:93-105. ...ochondrial cytochromes. Muscle mitochondria differed from kidney and liver mitochondria by having a higher ANT content relative to cytochrome content. Respiration
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• Gabrielova 2011 Abstract IOC61
  ...en V, Modriansky M (2011) Evaluation of an oxygen consumption in rat heart mitochondria treatment by polyphenol compounds. MiPNet16.01. |abstract=Reactive oxygen species (ROS) originating from mitochondria are perceived as a factor contributing to cell aging and means have been so
  2 KB (289 words) - 14:24, 13 February 2015
• Dixit 2017 Free Radic Biol Med
  ...d from wild-type mice increased oxygen consumption compared with untreated mitochondria suggesting ascorbate may support energy production. This study suggests tha |keywords=Alzheimer’s disease, Ascorbate, Energy production, Mitochondria, Oxidative stress
  3 KB (338 words) - 17:48, 20 December 2017
• Rousarova 2015 Abstract MiPschool London 2015
  |title=Estimation of ROS production in isolated mitochondria after treatment with an acetaminophen metabolite. ...r probe that is nonfluorescent until oxidized by ROS [3]. We used isolated mitochondria from rat liver and from kidney cells treated with mitochondrial substrates
  3 KB (412 words) - 09:59, 8 November 2016
• Lanza 2010 Curr Opin Clin Nutr Metab Care
  '''Recent findings''': Classic studies of isolated mitochondria have measured function from maximal respiratory capacity. These fundamental ...l tool for ''in vitro'' measurements of mitochondrial function in isolated mitochondria and permeabilized fibers.''' Direct measurements of ATP production are poss
  2 KB (277 words) - 15:44, 19 March 2015
• Tretter 2013 Abstract MiP2013
  ...alpha-ketoglutarate supported oxidation in isolated brain, heart and liver mitochondria. Mitochondr Physiol Network 18.08. ...s is supported by the Mma-induced changes in P/O ratio. In brain and heart mitochondria in the presence of Mma, P/O ratio of aKG oxidation decreases. This finding
  3 KB (460 words) - 11:25, 28 April 2017
• NSGp
  ::::* DL-Protocol for isolated mitochondria and tissue homogenate (mt): [[SUIT-038_O2_mt_D091]] ::::* DL-Protocol for isolated mitochondria and tissue homogenate (mt): [[SUIT-038_O2_mt_D091]]
  1 KB (156 words) - 14:03, 12 April 2023
• Tokarska-Schlattner 2007 Biochim Biophys Acta
  ...U (2007) Reduced creatine-stimulated respiration in doxorubicin challenged mitochondria: Particular sensitivity of the heart. Biochim Biophys Acta 1767:1276-84. ...ous, non-muscle MtCK isoenzyme. DXR impaired respiration of isolated heart mitochondria already after short-term exposure (minutes), affecting both ADP- and Cr-sti
  3 KB (340 words) - 16:35, 26 March 2018
• Mikulas 2020 Materials
  ...ylate- (TEGDMA-) treated dental pulp stem cells (DPSCs) and isolated brain mitochondria are amended by redox compound methylene blue. Materials (Basel) 13(16):3472 ...e present study, the bioenergetic effects of MB on TEGDMA-treated isolated mitochondria and on human dental pulp stem cells (DPSC) were analyzed.
  3 KB (360 words) - 12:00, 21 September 2020
• Bunik 2018 Biol Chem
  ...en peroxide-generating reactions of the complexes, established in isolated mitochondria. We assess available data on the use of modulations of enzyme activity to i
  2 KB (201 words) - 09:28, 23 August 2021
• Collard 2018 Mol Cell Biol
  ...that a cell-penetrating peptide can deliver active multimeric enzyme into mitochondria ''in vitro'', ''in situ'', and ''in vivo'' and push the size limit of intra |preparations=Isolated mitochondria
  2 KB (247 words) - 11:04, 13 March 2018
• Kandel 2019 Biophys J
  ...rate combination, optimal [Ca²⁺], and specific tissue source of mitochondria to achieve a level of energy metabolism and ATP production comparable to '' |preparations=Isolated mitochondria
  3 KB (432 words) - 16:01, 28 February 2019
• Laterveer 1996 FEBS Lett
  |abstract=Macromolecules were added to isolated rat liver mitochondria to mimic cytosolic macromolecules and tested for their effects on the ADP d |keywords=Adenylate kinase, Macromolecule, Mitochondria, Rat liver, Intermembrane space, Oxidative phosphorylation, Compartmentatio
  1 KB (190 words) - 14:27, 27 March 2018
• Monge 2008 Mol Cell Biochem
  ...Schlattner U, Vendelin M, Saks V (2008) Regulation of respiration in brain mitochondria and synaptosomes: restrictions of ADP diffusion in situ, roles of tubulin, ...ion in situ was rather high (110 ± 11lM) in comparison with isolated brain mitochondria
  2 KB (342 words) - 15:20, 26 March 2018
• Figueira 2012 Methods Mol Biol
  ...luorescent probe for the evaluation of mitochondrial membrane potential in isolated organelles and permeabilized cells. Methods Mol Biol 810:103-17. ...e the use of safranine as a fluorescent indicator of delta Psi in isolated mitochondria and digitonin-permeabilized cells. We present suitable conditions to employ
  2 KB (281 words) - 00:33, 18 February 2022
• Syringe\500 mm3 51/0.41 mm
  ...length, 0.41 mm inner diameter; for injections of suspensions of isolated mitochondria and filling of the [[Microsyringe\200 mm3\TIP2k]]. ...olated mitochondria: larger inner diameter avoids mechanical damage of the mitochondria.
  1 KB (200 words) - 11:09, 11 November 2020
• Sobotka 2017 Thesis
  ...most pronounced effect of α-tocopheryl succinate was apparent in isolated mitochondria and the least pronounced effect was observed in permeabilized hepatocytes. |preparations=Intact cells, Homogenate, Isolated mitochondria
  3 KB (369 words) - 17:57, 22 November 2017
• Shepherd 2023 J Am Chem Soc
  ...le=Shepherd RE, Kreinbrink AC, Njimoh CL, Vali SW, Lindahl PA (2023) Yeast mitochondria import aqueous fe^II and, when activated for iron-sulfur cluster ...y incorporated into cytosolic proteins. Iron from buffer was imported into mitochondria fastest, followed by mitochondrial ISC assembly, LMM iron export, and cytos
  3 KB (378 words) - 17:45, 30 June 2023
• Duicu 2017 Can J Physiol Pharmacol
  ...in the presence of methylene blue. In conclusion, in cardiac mitochondria isolated from diabetic and nondiabetic rat hearts, methylene blue improved respirato ...ds=Diabetes mellitus, Methylene blue, Mitochondrial respiration, Rat heart mitochondria, Reactive oxygen species
  2 KB (347 words) - 13:02, 7 March 2020
• Schmitt 2013 Anal Biochem
  ...Zischka H (2013) A semi-automated method for isolating functionally intact mitochondria from cultured cells and tissue biopsies. Anal Biochem 443:66-74. ...ere, we present an efficient method to isolate functionally active, intact mitochondria from cultured or primary cells and minute tissue samples in a rapid, highly
  2 KB (203 words) - 11:41, 15 March 2021
• Skemiene 2015 FEBS J
  ...ed state 3 respiration and ATP synthesis with NADH-dependent substrates in mitochondria after ischemia. The results suggest that certain anthocyanins can act as el |keywords=Anthocyanin, Cardioprotection, Heart ischemia, Mitochondria, Respiratory chain
  2 KB (279 words) - 04:47, 12 February 2020
• Rasmussen 2001 Pflugers Arch
  ...2001) The effect of high-intensity exhaustive exercise studied in isolated mitochondria from human skeletal muscle. Pflugers Arch 443:180-7. ...o be involved. Some effects may have been reversed during isolation of the mitochondria and the changes in mitochondrial function in situ upon exhaustion may have
  2 KB (303 words) - 23:29, 8 December 2019
• McFarlane 2017 Am J Physiol Regul Integr Comp Physiol
  ...s in isolated mitochondria, norepinephrine-stimulated respiration rates of isolated BAT adipocytes did not differ between torpor and IBE, perhaps because the a |keywords=Q10, Electron transport system, Hibernation, Mitochondria, Uncoupled thermogenesis
  3 KB (373 words) - 09:56, 29 November 2017
• Van Bree 2014 PhD Thesis
  |title=van Bree B (2014) Mitochondria, lipotoxicity and skeletal muscle metabolism: implications for type 2 diabe |info=[https://cris.maastrichtuniversity.nl/portal/en/publications/mitochondria-lipotoxicity-and-skeletal-muscle-metabolism--implications-for-type-2-diabet
  1 KB (175 words) - 16:46, 30 August 2018
• Davila 2018 Elife
  ...JA (2018) Nicotinamide adenine dinucleotide is transported into mammalian mitochondria. Elife 7:e33246. ...and ribose moieties results in the appearance of doubly labeled NAD within mitochondria. Analogous experiments with doubly labeled nicotinic acid riboside (labelin
  2 KB (261 words) - 18:04, 31 January 2022
• Danylovych 2016 Ukr Biochem J
  ...NADH indicates changes in oxidation state of these nucleotides in isolated mitochondria, which may be used to assay the potential of effectors of electron transpor |preparations=Isolated mitochondria
  2 KB (300 words) - 13:18, 14 February 2022
• Fisher 2020 J Physiol
  ...egnancies compared to healthy controls. Furthermore, by examining isolated mitochondria from the cytotrophoblast and syncytiotrophoblast cell lineages of the place |preparations=Isolated mitochondria
  2 KB (302 words) - 18:02, 19 October 2021
• Endlicher 2017 MiP2017
  ...:EndlicherR.jpg|left|90px|Rene Endlicher]] Age-related changes in isolated mitochondria from rat liver. ...e-related changes in Ca²⁺ ion effect on MPTP and the ability of mitochondria to accumulate calcium. We also measured changes in mitochondrial membrane p
  4 KB (523 words) - 11:23, 18 March 2020
• Bertero 2020 Circ Res
  |title=Bertero E, O'Rourke B, Maack C (2020) Mitochondria do not survive calcium overload during transplantation. Circ Res 126:784-86 |preparations=Isolated mitochondria
  736 bytes (86 words) - 16:17, 7 May 2020
• Takahashi 2018 Sci Rep
  ..., Ohsawa I, Shirasawa T, Takahashi M (2018) In vitro rejuvenation of brain mitochondria by the inhibition of actin polymerization. Sci Rep 8:15585. ...amount of CIII-bound cyt c increased significantly after treatment of the mitochondria with CB. In contrast, no significant alteration in the assembly or the CcO
  2 KB (265 words) - 17:37, 25 February 2020
• McKee 2010 Abstract IOC60
  ...ZT is a competitive inhibitor of thymidine phosphorylation in mitochondria isolated from heart, liver, and brain [1, 2] and that AZT significantly decreases th ...various time-points, the levels of all four dNTPs in the medium and in the mitochondria were measured using a template driven assay as described [3] that limits t
  3 KB (469 words) - 14:37, 8 November 2016
• Tretter 2007 Neurochem Res
  ...(2007) Moderate dependence of ROS formation on DeltaPsim in isolated brain mitochondria supported by NADH-linked substrates. Neurochem Res 32:569-75. ...ated to reverse electron flow observed in well-coupled succinate-supported mitochondria.
  2 KB (241 words) - 20:17, 7 April 2022
• Piwonska 2016 Acta Neurobiol Exp (Wars)
  ...lts confirm presence of BK_Ca channel in rat hippocampal neurons mitochondria and suggest putative role for mitoBK_Ca in neuroprotection. |keywords=Potassium channel, Glutamate, Hippocampal slice cultures, Mitochondria, Respiration, Patch-clamp
  2 KB (319 words) - 09:49, 8 November 2016
• DeLuca 1961 Proc Natl Acad Sci USA
  |title=DeLuca HF, Engstrom GW (1961) Calcium uptake by rat kidney mitochondria. Proc Natl Acad Sci USA 47:1744-50. ...de, cyanide, oligomycin, and iodoacetate at the concentrations tested. The mitochondria apparently can bind about 15 calcium ions per molecule of ATP utilized.
  1 KB (158 words) - 17:39, 23 November 2015
• Menezes-Filho 2018 Biochim Biophys Acta Bioenerg
  ...nez LA, Kowaltowski AJ (2018) Fasting promotes functional changes in liver mitochondria. Biochim Biophys Acta Bioenerg 1860:129-35. ...sting of rodents is commonly adopted in protocols to obtain isolated liver mitochondria, but the effects of fasting itself on mitochondrial function are poorly cha
  2 KB (221 words) - 12:14, 2 August 2023
• Sokolova 2009 BMC Cell Biol
  ...a N, Vendelin M, Birkedal R (2009) Intracellular diffusion restrictions in isolated cardiomyocytes from rainbow trout. BMC Cell Biol 10:90. ...iration in permeabilized fibers, permeabilized cardiomyocytes and isolated mitochondria from rainbow trout heart. Experiments were performed at 10, 15 and 20°C in
  3 KB (425 words) - 09:15, 9 November 2016
• Nabben 2011 Biochim Biophys Acta
  ...tion, acute lipotoxicity and oxidative damage in isolated skeletal muscle mitochondria from UCP3-ablated mice. Biochim Biophys Acta 1807:1095-105. ...quantifying 4-hydroxynonenal protein adducts and protein carbonyls in the mitochondria-but did not observe any protective effect of UCP3. We conclude that under t
  2 KB (308 words) - 16:24, 27 March 2018
• Brierley 1962 Proc Natl Acad Sci USA
  ...) Active transport of inorganic phosphate and magnesium ions by beef heart mitochondria. Proc Natl Acad Sci U S A 48:1928-35. |preparations=Isolated mitochondria
  693 bytes (99 words) - 11:18, 28 May 2015
• Dos Santos 2013 Bio Open
  ...content in cold-acclimated muscle mitochondria compared to warm-acclimated mitochondria (0.96±0.05 vs 0.68±0.02 nmol carboxyatractyloside mg−1 protein). This |keywords=Cold acclimation, Mitochondria, Skeletal muscle, Goldfish
  2 KB (281 words) - 17:49, 9 November 2017
• Carroll 2005 J Biol Chem
  ...as purified from thymus mitochondria by hydroxyapatite chromatography. The isolated protein was identified by peptide mass mapping and tandem mass spectrometry
  2 KB (260 words) - 16:07, 23 February 2015
• Hagl 2015 Neurochem Int
  ...ore efficient in preventing mitochondrial swelling in isolated mouse brain mitochondria and protecting PC12 cells from nitrosative stress than native curcumin. Due |preparations=Isolated mitochondria
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• Singer 1956 J Biol Chem
  |abstract=# Succinic dehydrogenase has been isolated from beef heart mitochondria as a soluble protein in a state approaching homogeneity by physi ...avin per mole of protein (200,000 gm.). The dehydrogenase may be isolated from aged starting material with 2 atoms of iron per mole and h
  2 KB (311 words) - 10:46, 6 July 2022
• Alleman 2016 Am J Physiol Heart Circ Physiol
  |abstract=Mitochondria influence cardiac electrophysiology through energy- and redox-sensitive ion ...simultaneous measurement of respiration and H₂O₂. Ex mitochondria sustained respiration with lower rates of H₂O₂ emissi
  3 KB (370 words) - 13:03, 7 March 2020
• Diaz-Ruiz 2008 J Biol Chem
  ...bP. This F16bP-mediated inhibition was also observed in isolated rat liver mitochondria, extending this process to mammalian cells. From these results and taking i |preparations=Isolated mitochondria
  2 KB (325 words) - 16:07, 27 August 2018
• Friederich-Persson 2013 Abstract MiP2013
  ...mately 120 mM in cardiac myocytes. Intracellular [K+] may therefore affect mitochondria function. ...amide and by 4-aminopyridine, respectively). On the contrary, RCR in heart mitochondria increased with increasing [K+] and was unaffected by K+channel inhibitors.
  2 KB (295 words) - 11:11, 28 April 2017
• Abou-Hamdan 2015 Methods Enzymol
  ...M, Blachier F, Bouillaud F (2015) Oxidation of H2S in mammalian cells and mitochondria. Methods Enzymol 554:201-28. ...ndria or cells and to summarize how the properties of this amazing couple (mitochondria and sulfide) translate into practical and conceptual consequences.
  2 KB (235 words) - 10:26, 9 November 2016
• Schiffer 2018 Am J Physiol Renal Physiol
  ...Kidney outer medulla mitochondria are more efficient compared with cortex mitochondria as a strategy to sustain ATP production in a suboptimal environment. Am J P ...ion. We therefore investigated functional differences between mitochondria isolated from kidney cortex and medulla of healthy normoglycemic rats by using high-
  2 KB (339 words) - 10:52, 17 September 2018
• Vuda 2012 Innate Immun
  ...eletal muscle mitochondrial respiration. Subsequent incubation of isolated mitochondria with norepinephrine and dobutamine did not significantly influence their re |preparations=Isolated mitochondria
  3 KB (361 words) - 11:03, 13 February 2015
• Oroboros 1994
  ...contributions to the conference proceedings, with applications on isolated mitochondria, cultured cells and permeabilized muscle tissue. ...n respirometric approach to |Oxygen kinetics|oxygen kinetics]] of isolated mitochondria and cultured cells:
  2 KB (250 words) - 14:45, 25 January 2020
• Aon 2010 Biochim Biophys Acta
  ...ss either when mitochondria become more uncoupled (i.e., low Psim) or when mitochondria are maximally reduced (as in ischemia or hypoxia). The continuum described
  2 KB (323 words) - 21:46, 24 September 2021
• Romeiro 2020 bioRxiv
  ...ported data using isolated mitochondria. The functional assessment of mWAT mitochondria through mild mechanical permeabilization, lipid depletion and high resoluti |keywords=Metabolism, Mitochondria, Bioenergetics, Respiration, Adipose, Obesity, Method
  2 KB (289 words) - 15:58, 24 February 2020
• Lukasiak A
  |area of interest=Isolated mitochondria, Intact cells, Permeabilized cells
  392 bytes (47 words) - 17:10, 12 February 2018
• Rolfe 1994 Biochim Biophys Acta
  ...phorylation was also assessed. The distribution of control in mitochondria isolated from the four tissue types was found to be similar.
  2 KB (269 words) - 12:36, 24 November 2022
• Kosenko 2010 Biology Bull
  ...ses in rat liver mitochondria has been studied. The addition of hypoxen to mitochondria caused a reduction of the rate of phosphorylating and uncoupling respiratio ...ates. The activities of malate, glutamate, and succinate dehydrogenases in mitochondria were significantly decreased by the effect of hypoxen.
  1 KB (161 words) - 15:50, 26 May 2015
• Mathers 2017 J Comp Physiol B
  ...s in homogenized isolated mitochondria and whole liver tissue. In isolated mitochondria, activities of complexes I and II were significantly lower in torpor relati |keywords=Metabolic suppression, Mitochondria, Mitochondrial respiration, Oxidative phosphorylation
  2 KB (343 words) - 11:27, 13 November 2017
• Johansen 2011 Acta Physiol Scand
  '''Methods:''' Langendorff perfused rat hearts, isolated mitochondria and isolated myocytes were used. Infarct size, mitochondrial respiration, time to mitoch ...(2 mm). Laser scanning confocal microscopy of tetramethylrhodamine-loaded isolated rat myocytes using line scan mode showed that Hp increased time to MPTP ope
  2 KB (290 words) - 17:07, 19 February 2018
• Nord 2023 FASEB J
  ...ke a whole blood approach unless specific circumstances require the use of isolated blood cells. ...rds=Blood, Blood cells, Cell respiration, Energy, Erythrocyte, Metabolism, Mitochondria
  2 KB (335 words) - 16:26, 8 February 2023
• Kunz 1993 Biochim Biophys Acta
  ...pathies these fibers are a suitable object for the replacement of isolated mitochondria in the diagnosis of mitochondrial myopathies and encephalomyopathies.
  2 KB (279 words) - 11:02, 27 March 2018
• Radogna 2021 Methods Mol Biol
  ...adient) centrifugation and depict major challenges to achieve "functional" mitochondria as basis for comprehensive physiological studies.
  2 KB (205 words) - 23:44, 23 April 2023
• Puchowicz 2004 Mitochondrion
  ...sis: assessing the integrated functional activity of human skeletal muscle mitochondria-case studies. Mitochondrion 4:377-85. ...t would not be apparent from ETC assays performed on detergent-solubilized mitochondria.
  2 KB (233 words) - 12:09, 9 December 2021
• Komary 2010 Biochim Biophys Acta
  ...effect of calcium on reactive oxygen species generation in isolated brain mitochondria. Biochim Biophys Acta 1797:922-8. ...ced depolarization mitochondria became more polarized. In highly polarized mitochondria, in the presence of ATP or oligomycin, where no hyperpolarization follows t
  3 KB (425 words) - 17:45, 31 January 2022
• Wollenberger 1963 Exp Mol Pathol
  ...ollenberger A, Kleitke B, Raabe G (1963) Some Metabolic Characteristics of Mitochondria from Chronically Overloaded, Hypertrophied Hearts. Exp Mol Pathol 2:251-60. ...seen from the data presented below, the loss in the inner structure of the mitochondria
  2 KB (226 words) - 11:34, 26 February 2015
• Cervinkova 2008 Physiol Res
  ..., Drahota Z (2008) Inhibition of palmityl carnitine oxidation in rat liver mitochondria by tert-butyl hydroperoxide. Physiol Res 57:133-6. ...other respiratory chain complexes. We present additional data on isolated mitochondria showing that palmityl carnitine oxidation is strongly depressed at a low (2
  2 KB (223 words) - 11:12, 18 March 2020
• MiPNet20.07 IsolationRatBrain-mt
  |title=Laboratory Protocol: isolation of rat brain mitochondria.[[Image:O2k-Protocols.jpg|right|80px|link=http://wiki.oroboros.at/index.php ...ntana M, Krumschnabel G (2016) Laboratory protocol: Isolation of rat brain mitochondria. Mitochondr Physiol Network 20.07(02):1-2.
  1 KB (137 words) - 06:51, 20 December 2023
• Goldberg 2019 Biochem J
  ...of a novel biochemical tool for investigating BCKA metabolism in isolated mitochondria. ...=Buffer C, Amino acid metabolism, Bioenergetics, Electron transport chain, Mitochondria
  3 KB (383 words) - 12:35, 4 June 2019
• Mahdaviani 2015 Fatty Acid Oxidation O2k-Network Discussion Forum
  |preparations=Isolated mitochondria
  378 bytes (47 words) - 11:25, 8 November 2016
• Tomar 2022 Biochim Biophys Acta Bioenerg
  ...ay play a more prominent regulatory role in heart mitochondria than kidney mitochondria. These studies provide novel quantitative data demonstrating that the choic |preparations=Isolated mitochondria
  3 KB (386 words) - 11:58, 27 March 2023
• Lund 2015 Fatty Acid Oxidation O2k-Network Discussion Forum
  |preparations=Homogenate, Isolated mitochondria
  372 bytes (48 words) - 11:25, 8 November 2016
• Labieniec 2009 Chem Biol Interact
  ...cylidene aminoguanidine (RAG) on selected parameters of isolated rat liver mitochondria. Chem Biol Interact 179:280-7. ...d merely when monitored in the presence of ADP. The reduced sensitivity of mitochondria to calcium-induced depolarization was significant only at higher RAG concen
  3 KB (328 words) - 15:09, 20 March 2015
• Kabiri 2021 Methods Mol Biol
  ...rne C, Fontes A, Knolle PA, Zischka H (2021) Isolation and Purification of Mitochondria from Cell Culture for Proteomic Analyses. Methods Mol Biol 2261:411-419. ...t quality and coverage of the mitochondrial proteome of crude and purified mitochondria from cultured liver cancer cell lines.
  1 KB (169 words) - 11:54, 16 August 2021
• Brand 2011 Biochem J
  ...ake ATP than do measurements of intermediates and potentials. For isolated mitochondria, the best assay is mitochondrial respiratory control: the increase in respi
  2 KB (240 words) - 12:11, 16 September 2021
• Kleinwaechter 2022 RSC Chem Biol
  ...ereau T, Osiewacz HD, Schneider D (2022) CLiB - a novel cardiolipin-binder isolated via data-driven and ''in vitro'' screening. RSC Chem Biol 3:941-54. https:/ ...on of cardiolipin-containing intact bacterial cells as well as of isolated mitochondria. Thus, the structure and function of mitochondrial membranes and membrane p
  2 KB (264 words) - 17:05, 13 March 2024
• Kollberg 2015 Fatty Acid Oxidation O2k-Network Discussion Forum
  |preparations=Isolated mitochondria
  361 bytes (45 words) - 11:24, 8 November 2016
• Goldberg 2021 Biochem J
  ...of a novel biochemical tool for investigating BCKA metabolism in isolated mitochondria. ...=Buffer C, Amino acid metabolism, Bioenergetics, Electron transport chain, Mitochondria
  3 KB (380 words) - 15:51, 14 July 2021
• FNS
  ...exerts an [[additive effect of convergent electron flow]] in most types of mitochondria. ::::* DL-Protocol for isolated mitochondria and tissue homogenate (mt):[[SUIT-002 O2 mt D005]]
  3 KB (403 words) - 19:58, 1 January 2021
• Lund 2012 Abstract IOC68
  ...intensity exercise prevents impairment of respiratory capacity in cardiac mitochondria from obese mice. MiPNet17.08. ...t of high intensity training (HIT) on mitochondrial respiration in cardiac mitochondria from obese mice. Because long chain fatty acids are supposed to increase LE
  3 KB (398 words) - 17:07, 19 February 2018
• Friedrich 2010 Abstract MiP2010
  ...x mitochondria are non-functional in a potassium-based media whereas heart mitochondria improve with increasing potassium concentration. Mitochondr Physiol Network ...ffect of different [K⁺] on respiratory function in mitochondria isolated from kidney cortex and heart of healthy male Sprague-Dawley rats.
  3 KB (471 words) - 10:40, 9 November 2016
• Fisher 2020 Placenta
  ...on and preliminary biochemical characterisation of these discrete forms of mitochondria. ...ls and smaller mitochondria from the syncytiotrophoblast. Freshly isolated mitochondria were assessed for Complex I and Complex II respiration and maximal respirat
  3 KB (354 words) - 16:09, 3 June 2020
• Serna 2022 MitoFit
  ...owski AJ (2022) Measuring mitochondrial Ca²⁺ efflux in isolated mitochondria and permeabilized cells. https://doi.org/10.26124/mitofit:2022-0021 — ''2 ...022_Mitofit.pdf Measuring mitochondrial Ca²⁺ efflux in isolated mitochondria and permeabilized cells]<br/>
  3 KB (458 words) - 07:51, 8 January 2023
• Hoppel 2012 Abstract Bioblast
  ...oppel CL (2012) Mitochondrial functional testing – muscle biopsy, isolated mitochondria and others. Mitochondr Physiol Network 17.12. ...an be assessed (measured) as oxidative phosphorylation in freshly isolated mitochondria.
  4 KB (545 words) - 09:27, 24 November 2015
• Kudin 2004 J Biol Chem
  ...WS (2004) Characterization of superoxide-producing sites in isolated brain mitochondria. J Biol Chem 279:4127-35. ...flavine mononucleotide moiety. Because short-term incubation of rat brain mitochondria with H₂O₂ induced increased H₂O_{2</sub
  3 KB (399 words) - 21:39, 13 April 2022
• Makrecka 2013 Abstract MiP2013
  ...wn that excessive exogenous FA cause mitochondrial dysfunction in isolated mitochondria and could play a detrimental role in myocardial ischemia-reperfusion injury ...fter left anterior descending coronary artery occlusion. Mitochondria were isolated from the non-risk area and the area at risk to measure radiolabeled FA. In
  4 KB (529 words) - 14:24, 27 March 2018
• Yamamoto 2018 Arch Biochem Biophys
  ...uch a difference in this potency was close to that in the case of isolated mitochondria, signifying that the PEI-induced permeabilization of mitochondrial membrane |keywords=Cytochrome c, Lipid phase, Mitochondria, Permeability transition, Polyethyleneimine
  2 KB (287 words) - 11:25, 6 August 2018
• Hagland 2015 Fatty Acid Oxidation O2k-Network Discussion Forum
  |preparations=Intact cells, Isolated mitochondria
  386 bytes (50 words) - 11:06, 8 November 2016
• Rasmussen 2001 Am J Physiol Endocrinol Metab
  ...of human quadriceps muscle: in vivo data parallel measurements on isolated mitochondria. Am J Physiol Endocrinol Metab 280:E301-7. ...succinate dehydrogenase, and pyruvate dehydrogenase, measured in isolated mitochondria, corresponded to, respectively, 15, 3, and 1.1 times the rates calculated f
  3 KB (423 words) - 17:56, 16 January 2021
• MiPNet20.06 IsolationMouseHeart-mt
  ...O2k-Protocols|O2k-Protocols]]Laboratory protocol: isolation of mouse heart mitochondria. ...rdoso LHD, Gnaiger E (2021) Laboratory protocol: Isolation of mouse heart mitochondria. Mitochondr Physiol Network 20.06(02):1-4.
  1 KB (135 words) - 07:10, 20 December 2023
• Korge 2008 Circ Res
  ...P, Weiss JN (2008) Reactive oxygen species production in energized cardiac mitochondria during hypoxia/reoxygenation: modulation by nitric oxide. Circ Res 103(8):8 ...xia sufficient to cause partial respiratory inhibition is more damaging to mitochondria than near-anoxia; b) •NO suppresses ROS-induced damage to electron transp
  2 KB (300 words) - 09:33, 12 April 2022
• P/O ratio
  |preparations=Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP
  436 bytes (57 words) - 18:49, 30 December 2020
• Moore 2015 PhD Thesis
  ...control ratios were depressed by approximately 30% compared to non-frozen mitochondria, which indicated a limited retention of at least some inner-membrane-depend |preparations=Isolated mitochondria
  3 KB (423 words) - 15:26, 13 November 2017
• Hoppel 2016a Abstract MitoFit Science Camp 2016
  |title=Skeletal muscle mitochondria in diagnostic studies. ...freshly isolated mitochondria and in other preparations with intact, fresh mitochondria.
  4 KB (546 words) - 15:34, 20 October 2017
• Funk 2015 Abstract MiPschool Greenville 2015
  |title=Isolated mitochondria from liver and brain of long-lived growth hormone receptor gene disrupted m ...a Seahorse XFe24 Analyzer. In ATP coupled experiments, we determined that mitochondria from GHR-/- livers and brain have reduced State 3 respiration in both the c
  3 KB (415 words) - 08:38, 21 July 2015
• Dubinin 2021 Free Radic Biol Med
  ...ina EI, Davletshin EV, Belosludtseva NV, Spivak AY, Belosludtsev KN (2021) Mitochondria-targeted prooxidant effects of betulinic acid conjugated with delocalized l ...ion of vesicle membranes. The paper discusses the relationship between the mitochondria-targeted effects of F16-betulinic acid conjugates and their cytotoxicity.
  3 KB (362 words) - 15:05, 19 July 2021
• Davis 2023 MitoFit
  ...that Magnesium Green has no significant effect on respiration of isolated mitochondria, whereas tetramethylrhodamine inhibits phosphorylating respiration and incr |preparations=Isolated mitochondria
  2 KB (249 words) - 16:34, 30 April 2024
• Vajrala 2016 Integr Biol (Camb)
  ...Arbault S (2016) PDMS microwells for multi-parametric monitoring of single mitochondria on a large scale: a study of their individual membrane potential and endoge ...pproach to better characterize the individual metabolic status of isolated mitochondria, possibly as a function of their cell or organ origin or in different physi
  2 KB (314 words) - 11:06, 14 February 2022
• Shabalina 2013 Abstract IOC79
  ...s ROS production from forward electron transport was increased in isolated mitochondria from mtDNA mutator mice. This pattern of ROS production was associated with |keywords=mitochondria, heart liver and skeletal muscle, mtDNA mutation, mitochondrial ROS product
  3 KB (378 words) - 16:01, 13 November 2017
• Kulawiak 2008 Exp Neurol
  ...WS (2008) BK channel openers inhibit ROS production of isolated rat brain mitochondria. Exp Neurol 212:543-7. ...nductance potassium channel being localised in the inner membrane of brain mitochondria inhibits ROS production by respiratory chain complex I. This finding is sug
  2 KB (273 words) - 16:39, 17 February 2015
• Borras 2010 Biochim Biophys Acta
  ...J (2010) Direct antioxidant and protective effect of estradiol on isolated mitochondria. Biochim Biophys Acta 1802:205-11. ...of reactive oxygen species and preventing the release of cytochrome c from mitochondria.
  2 KB (244 words) - 16:39, 12 October 2016
• Volska 2018 MiP2018
  ...ntify the mechanisms of long-chain acylcarnitine accumulation in the heart mitochondria during ischemia. ...xidation, concentrations of acyl-CoA and CoA were measured in mitochondria isolated from the non-risk area and the area at risk of ischemic hearts.
  3 KB (341 words) - 12:16, 27 August 2018
• Rostovtseva 2008 Proc Natl Acad Sci U S A
  ...icant length or anionic character are only found in cells that do not have mitochondria. |preparations=Isolated mitochondria
  3 KB (363 words) - 19:01, 16 January 2021
• Sobotka 2016 J Bioenerg Biomembr
  ...the latter. We also measured the effect of 3-BP on respiration of isolated mitochondria. ADP-activated respiration was inhibited by 20 μM 3-BP within 10 min. Simi |keywords=3-bromopyruvate, Hepatocyte, Liver, Mitochondria, Toxicity
  2 KB (328 words) - 10:17, 8 November 2016
• McLaughlin 2020b Sci Rep
  ...ed comparison of functional parameters between populations of mitochondria isolated from metabolically distinct tissues. This method can easily be applied acro |preparations=Isolated mitochondria
  2 KB (285 words) - 19:00, 29 October 2020
• Tretter 2014 Free Radic Biol Med
  ...es the beneficial bioenergetic effects of methylene blue in isolated brain mitochondria. Free Radic Biol Med 77:317-30. .../sub>O₂ should be considered for the overall oxidative state of mitochondria treated with MB.
  3 KB (385 words) - 13:44, 7 March 2020
• Chicco 2013 Abstract IOC79
  ...ific impairment of oxidative phosphorylation in Tafazzin-deficient cardiac mitochondria: potential role of CoA deficiency. Mitochondr Physiol Network 18.07 ...irment of carbohydrate and lipid oxidation, perhaps due to a deficiency in mitochondria CoA that deserves further investigation.
  3 KB (425 words) - 15:57, 7 November 2016
• Debska 2002 Biochim Biophys Acta
  .... Since both diazoxide and nicorandil facilitated swelling of de-energised mitochondria in KSCN buffer at the same concentrations, our results implicate the presen |preparations=Isolated mitochondria
  2 KB (265 words) - 15:45, 24 March 2015
• Mela 1979 Methods Enzymol
  ...le=Mela L, Seitz S (1979) Isolation of mitochondria with emphasis on heart mitochondria from small amounts of tissue. Methods Enzymol 55:39-46. ...suffice. This method has been used successfully for the isolation of heart mitochondria from several species of adult and newborn animals as well as human samples.
  2 KB (235 words) - 11:34, 27 May 2015
• Friederich-Persson 2020 Am J Physiol Renal Physiol
  ...tochondrial angiotensin II receptors regulate oxygen consumption in kidney mitochondria from healthy and type 1 diabetic rats. Am J Physiol Renal Physiol 318:F683- ...dy investigated the role of Ang II signaling in kidney cortex mitochondria isolated from control and streptozotocin-induced diabetic rats. Mitochondrial oxygen
  3 KB (364 words) - 09:48, 10 March 2022
• St-Pierre 2000 Proc Natl Acad Sci U S A
  |title=St-Pierre J, Brand MD, Boutilier RG (2000) Mitochondria as ATP consumers: Cellular treason in anoxia. Proc Natl Acad Sci U S A 97:8 |abstract=In anoxia, mitochondria change from being ATP producers to
  2 KB (263 words) - 09:30, 9 November 2016
• Schiffer 2018 PLoS One
  ...mitochondrial function should to be considered when studying mitochondria isolated from animals anesthesized with inactin. |preparations=Isolated mitochondria
  2 KB (271 words) - 13:32, 7 March 2020
• Rauchova 2011 Horm Metab Res
  ...cerol-3-phosphate dehydrogenase expression and oxygen consumption in liver mitochondria of female and male rats with chronic alteration of thyroid status. Horm Met ...rol-3-phosphate-dependent oxygen consumption of freshly isolated rat liver mitochondria from the TH animals was higher in comparison with the EU animals and it was
  2 KB (325 words) - 12:36, 19 March 2015
• Van Doan 2023 Abstract IOC162
  |title=Van Doan K, Luongo TS, Mukherjee S, Baur JA (2023) Mouse cardiac mitochondria are resilient to Nampt-dependent NAD⁺ los. Mitochondr Physiol Ne |abstract=Mitochondria are important in cardiac metabolism, generating most of the ATP (>95%) and
  2 KB (292 words) - 17:40, 18 September 2023
• Iglesias-Gonzalez 2013 J Neurosci Methods
  ...o-Otero R, Mendez-Alvarez E (2013) A simple method for isolating rat brain mitochondria with high metabolic activity: Effects of EDTA and EGTA. J Neurosci Methods ...r metabolic and oxidative stress experiments with fresh isolated rat brain mitochondria.
  3 KB (349 words) - 14:26, 3 June 2020
• Hoppel 2016b Abstract MitoFit Science Camp 2016
  |title=Comparison of permeabilized skeletal muscle fibers or isolated mitochondria for the detection of oxidative phosphorylation defects. ...l muscle. During an one year period we used both isolated skeletal muscle mitochondria and permeabilized fibers from the same biopsy to evaluate OXPHOS capacity.
  3 KB (452 words) - 18:44, 10 January 2022
• Munro 2013 Aging Cell
  ...N, Paquin F, Kemeid V, Blier PU (2013) Low hydrogen peroxide production in mitochondria of the long-lived ''Arctica islandica'': underlying mechanisms for slow agi ...II activity can contribute to the lower ROS production of ''A. islandica'' mitochondria, in accordance with previous studies. We further propose that a lower compl
  3 KB (376 words) - 16:44, 21 November 2018
• Legkun 2014 Abstract IOC 2014-04 Schroecken
  ...ture (PCP, FCCP et al.) simultaneously with membrane potential of isolated mitochondria. |preparations=Isolated mitochondria
  2 KB (221 words) - 09:43, 3 June 2020
• Manakov 2018 J Physiol Biochem
  ...left ventricles exhibited two times higher respiratory activity than those isolated from the right ventricles. Whereas, we did not observe any significant chan |keywords=CD36, Heart, Left and right ventricles, Mitochondria, OXPHOS, SHR
  2 KB (299 words) - 13:32, 11 May 2020
• Kamath 2014 J Biol Chem
  ...MP synthesized by TK2 over medium TMP and that this is disrupted in broken mitochondria. |keywords=Mitochondria, Mitochondrial metabolism, Nucleoside/nucleotide biosynthesis, Nucleoside/n
  2 KB (355 words) - 14:33, 12 October 2021
• Gellerich 2002 Biochim Biophys Acta (Vol 1554)
  ...quantitation of ADP diffusion gradients across the outer membrane of heart mitochondria in the presence of macromolecules. Biochim Biophys Acta 1554:48-56. ...ction of cytosolic macromolecules on mitochondria. Under these conditions, mitochondria have a more native, condensed configuration.
  3 KB (388 words) - 10:54, 23 January 2019
• Rosca 2011 Biochim Biophys Acta
  |title=Rosca M, Minkler P, Hoppel CL (2011) Cardiac mitochondria in heart failure: Normal cardiolipin profile and increased threonine phosph ...zed cardiolipin molecular species were not observed. In heart mitochondria isolated from HF, Complex IV not incorporated into respirasomes exhibits increased t
  2 KB (303 words) - 09:12, 16 June 2015
• Monteiro 2019 J Bioenerg Biomembr
  ...by multiple substrates and calcium challenge reveal dysfunctions in brain mitochondria in a model related to acute psychosis. J Bioenerg Biomembr 52:1-15. ...se data favor the hypothesis that disruption of key physiological roles of mitochondria may be a trigger in acute psychosis and, consequently, schizophrenia.
  3 KB (396 words) - 12:43, 10 March 2020
• Varikmaa 2013 J Bioenerg Biomembr
  ...ticulum in cardiac cells, known to contain 5,000-10,000 individual, single mitochondria, which are regularly arranged at the level of sarcomeres and are at Z-lines |keywords=Cardiac cells, Respiration, Mitochondria, Fusion-fission, Calcium metabolism, Energy metabolism
  3 KB (365 words) - 15:16, 9 November 2016
• Galli 2013 J Exp Biol
  ...) or State IV respiration. Results from this study demonstrate that turtle mitochondria remodel in response to chronic anoxia exposure and a reduction in Complex V |keywords=Anoxia, Electron transport chain, Heart, Hypoxia, Mitochondria, Oxidative phosphorylation, Proton conductance, Turtle
  3 KB (344 words) - 10:24, 9 November 2016
• Justo 2005 Am J Physiol Cell Physiol
  ...ender differences in the aforementioned parameters were more patent in the isolated heavy M1 and M3 mitochondrial fractions. The present study demonstrates tha |preparations=Isolated mitochondria
  2 KB (287 words) - 17:12, 19 February 2018
• OctPGMSGp pathway control state
  ::::* DL-Protocol for isolated mitochondria and tissue homogenate (mt): [[SUIT-002 O2 mt D005]] ::::* DL-Protocol for isolated mitochondria and tissue homogenate (mt): [[SUIT-002 O2 mt D005]]
  1 KB (176 words) - 05:02, 19 July 2022
• Montaigne 2011 Mitochondrion
  ...tile dysfunction and depression of mitochondrial respiration. Mitochondria isolated from doxorubicin-treated human atrial trabeculae displayed reduced transmem |preparations=Isolated mitochondria
  1 KB (177 words) - 11:48, 22 December 2020
• Hickey 2015 Fatty Acid Oxidation O2k-Network Discussion Forum
  ...h fatty acid free BSA at 5:1 (PC:BSA). Worked well with fish and rat heart mitochondria, and permeabilised fibres. |preparations=Permeabilized tissue, Isolated mitochondria
  717 bytes (100 words) - 17:24, 27 March 2018
• Pereira 2020 PLoS One
  ...also observed more Drp1, a mitochondrial fission protein, in end-ischemia mitochondria. IPC failed to prevent this increase but did decrease mitochondrial-associa |preparations=Isolated mitochondria
  3 KB (362 words) - 18:58, 2 July 2020
• Grimpo 2014 Comp Biochem Physiol A Mol Integr Physiol
  ...es is not triggered by depression of substrate oxidation as found in liver mitochondria from other cold-exposed rodents. |preparations=Isolated mitochondria
  2 KB (327 words) - 11:08, 27 March 2018
• Schwarzer 2013 Am J Physiol Heart Circ Physiol
  ...entially affects respiratory capacity in interfibrillar and subsarcolemmal mitochondria. Am J Physiol Heart Circ Physiol 304:529-37. ...l mitochondrial content. Electron microscopy revealed normal morphology of mitochondria but reduced total mitochondrial volume density. In conclusion, IFM show gre
  3 KB (341 words) - 11:02, 26 February 2015
• Rasmussen 1997 Mol Cell Biochem
  ...smussen HN, Rasmussen UF (1997) Small scale preparation of skeletal muscle mitochondria, criteria of integrity, and assays with reference to tissue function. Mol C ...biceps femoris mitochondria prepared by the same method. The two types of mitochondria show many similarities. Some differences may be explained by a different am
  2 KB (309 words) - 01:21, 16 February 2022
• Correa 2012 Mitochondrion
  ...n on permeabilized muscle fibers, hydrogen peroxide production of isolated mitochondria and mtDNA copy number of whole individuals. The results show that a small s |preparations=Permeabilized tissue, Isolated mitochondria
  1 KB (189 words) - 17:26, 9 November 2017
• Arandarcikaite 2017 Abstract MITOEAGLE Barcelona
  Age-related responses of brain mitochondria to ischemic insult. [[Media:Odeta methods.pdf|Arandarcikaite_Presenation]] ...onths, whereas calcium retention capacity after ischemia decreased only in mitochondria from cerebellum of mature adults and aging rats. These data revealed age-de
  3 KB (366 words) - 12:13, 12 January 2018
• Bosma 2012 Histochem Cell Biol
  ...(2012) The lipid droplet coat protein perilipin 5 also localizes to muscle mitochondria. Histochem Cell Biol 137:205-16. ...at oxidation is higher upon PLIN5 overexpression. The presence of PLIN5 in mitochondria helps to understand why PLIN5, in contrast to PLIN2, is of specific importa
  3 KB (382 words) - 15:50, 12 March 2015
• Schwarzkopf 2013 Pharmazie
  ...nvolves a mechanism in which the drug reverses ischemia-induced changes in mitochondria, leading to a reduction of glutamate release. |preparations=Isolated mitochondria
  2 KB (235 words) - 10:49, 27 March 2018
• Schoettl T
  |topics=Mature white adipocytes, isolated white adipocyte mitochondria, Insulin resistance
  516 bytes (60 words) - 11:49, 13 February 2018
• Dubinin 2021 Membranes (Basel)
  ...of the mitochondrial electron transport chain. The paper discusses how the mitochondria-targeted effects of the F16-betulin conjugate may be related to its cytotox |keywords=F16, Betulin, Thymocytes, Mitochondria, Oxidative phosphorylation, ROS
  2 KB (310 words) - 12:42, 6 October 2021
• Endlicher 2009 Physiol Res
  ...roxide (BHP) inhibitory effect on oxygen consumption in isolated rat liver mitochondria was measured in the presence of various respiratory substrates. Strong inhi |keywords=Liver mitochondria, Mitochondrial enzymes, Peroxidative damage, Tert-butyl hydroperoxide
  2 KB (223 words) - 11:12, 18 March 2020
• GM-pathway control state
  ...control state) is established when glutamate&malate are added to isolated mitochondria, permeabilized cells and other mitochondrial preparations. Glutamate and tr ...% higher than with [[PM]] (PM_''P''). These results on isolated mitochondria agree with permeabilized fibres, although there are reports on respiratory
  2 KB (249 words) - 15:32, 2 August 2023
• MiPNet13.06 MiPArt-04
  |preparations=Intact cells, Isolated mitochondria
  439 bytes (40 words) - 09:52, 9 November 2016
• Hoejeberg 1977 Biochem Biophys Res Commun
  ...titutively active nicotinamide nucleotide transhydrogenase from beef heart mitochondria. Biochem Biophys Res Commun 78:1183-90. |abstract=Nicotinamide nucleotide transhydrogenase from beef heart mitochondria was purified to homogeneity and characterized. The enzyme is devoid of othe
  1 KB (148 words) - 12:45, 24 February 2015
• Borutaite 2014 Abstract MiP2014
  ...c plant compounds (anthocyanins), addition of cytochrome ''c'' to isolated mitochondria results in acute stimulation of mitochondrial respiration. This effect is n |preparations=Isolated mitochondria
  2 KB (336 words) - 08:23, 16 June 2015
• Siekevitz 1956 J Biophys Biochem Cytol
  |preparations=Isolated mitochondria
  499 bytes (64 words) - 10:34, 28 May 2015
• Skalska 2008 Biochim Biophys Acta
  ...M, Szewczyk A, Kunz WS (2008) A novel potassium channel in skeletal muscle mitochondria. Biochim Biophys Acta 1777:651-9. ...m channel in skeletal muscle mitochondria. In isolated rat skeletal muscle mitochondria, Ca(2+) was able to depolarize the mitochondrial inner membrane and stimula
  2 KB (271 words) - 12:07, 7 October 2015
• Milerova 2016 Mol Cell Biochem
  ...r or voltage-dependent anion channel. Increased resistance of female heart mitochondria thus cannot be explained by changes in putative components of MPTP, and rat |preparations=Isolated mitochondria
  3 KB (373 words) - 09:30, 8 November 2016
• Makrecka-Kuka 2016 Abstract MitoFit Science Camp 2016
  ...palmitoyl-CoA and palmitoylcarnitine were studied in isolated rat cardiac mitochondria. To study the effects ''in vivo'', pharmacological regulation of the availa ...tent. The pharmacological reduction of long-chain acylcarnitine content in mitochondria decreases ischemia-reperfusion induced mitochondrial dysfunction and signif
  2 KB (290 words) - 11:26, 8 November 2016
• Kucharska 2000 Physiol Res
  ...O, Zlatos L, Gvozdjáková A (2000) Deficit of coenzyme Q in heart and liver mitochondria of rats with streptozotocin-induced diabetes. Physiol Res 49:411-8. ...metabolism in diabetic rats. An important finding is that heart and liver mitochondria from the diabetic rats contain less CoQ9 and CoQ10 in comparison with the c
  3 KB (358 words) - 10:33, 7 May 2015
• King 2016 Redox Biol
  ...of respiration compared with pair-fed controls. In contrast, mitochondria isolated from the livers of SAM treated alcohol-fed rats showed no change in the sen |keywords=Alcohol, Hypoxia, Liver, Mitochondria, Nitric oxide, S-Adenosylmethionine
  3 KB (380 words) - 12:09, 8 November 2016
• Wagenaars 2014 Abstract IOC 2014-04 Schroecken
  |abstract=Mitochondria are crucially involved in cellular Ca2+ and redox homeostasis and apoptosis ...kinin-stimulated O2 consumption was less in fibroblasts from patients with isolated CI deficiency than in cells from a healthy individual.
  3 KB (352 words) - 15:46, 9 November 2016
• McFarlane 2016 Thesis
  ...torpor and IBE, regardless of assay temperature. The data suggest that BAT mitochondria become less temperature sensitive during torpor, allowing sustained functio ...ords=Uncoupled thermogenesis, Q10, electron transport system, Hibernation, Mitochondria, High resolution respirometry, Thirteen-lined ground squirrel
  2 KB (263 words) - 15:17, 13 November 2017
• Ziegler 1962 Arch Biochem Biophys
  ...em XLIII. The isolation of a succinic-coenzyme Q reductase from beef heart mitochondria. Arch Biochem Biophys 97:41-50. |abstract=The isolation of the succinic-CoQ reductase from beef heart mitochondria is described. The flavine content of the preparation is 4.8 mμmoles/mg. pr
  1 KB (199 words) - 11:04, 13 February 2015
• Appaix 2003 Exp Physiol
  ...ssible role of cytoskeleton in intracellular arrangement and regulation of mitochondria. Exp Physiol 88:175-90. ...tion and control of mitochondrial function in the cells ''in vivo'', where mitochondria are incorporated into functional complexes with sarcomeres and sarcoplasmic
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• Jung 1989 Anal Biochem
  ...DW, Davis MH, Brierley GP (1989) Estimation of matrix pH in isolated heart mitochondria using a fluorescent probe. Anal Biochem 178:348-54. ...relating the fluorescence ratio to pH is constructed using detergent-lysed mitochondria and the excitation maximum of 500 nm for BCECF in aqueous solution. Estimat
  2 KB (259 words) - 00:38, 18 February 2022
• Stankova 2017 MiP2017
  ...le=[[Image:StankovaP.jpg|left|90px|Pavla Stankova]] Isolation of rat liver mitochondria for bioenergetic studies. ...and buffers composition. Utilization of a standardized procedure protocols allows achieving valid data and provides necessary tool for comparison of results
  4 KB (548 words) - 10:56, 18 March 2020
• Wilson 2018 Virginia Tech
  ...=Wilson ZT, Perry JB, Brow DA (2018) High-resolution respirometry of heart mitochondria in healthy and stressed states. Virginia Tech. ...tioxidant which reduces reactive oxygen species. In this project, isolated mitochondria are treated with hydrogen peroxide with or without NAC. If NAC is capable o
  3 KB (361 words) - 15:49, 22 August 2018
• Dungel 2015 Free Radic Biol Med
  .... We show in a model of isolated rat liver mitochondria that upon hypoxia, mitochondria reduce nitrite to nitric oxide (NO) in amounts sufficient to inactivate red |keywords=Cytochrome c, Cytoprotection, Iron ions, Lipid peroxidation, Mitochondria, Nitrite, Rats
  2 KB (256 words) - 17:53, 9 November 2017
• Guerrero 2010 Mol Cell Biochem
  ...sible interactions of tubulin, microtubular network, and STOP protein with mitochondria in muscle cells. Mol Cell Biochem 337:239-49. ...od of kindred DNA. The results of sequencing and bioinformatic analysis of isolated cDNA pool common for heart and ''m. soleus'' showed that in adult mice the
  2 KB (346 words) - 16:56, 6 September 2022
• Maddalena 2017 Biochim Biophys Acta
  |title=Maddalena LA, Ghelfi M, Atkinson J, Stuart JA (2017) The mitochondria-targeted imidazole substituted oleic acid 'TPP-IOA' affects mitochondrial b ...eroxidase activity, TPP-IOA perturbs oxidative phosphorylation in isolated mitochondria. In live SH-SY5Y cells, TPP-IOA partially collapsed mitochondrial membrane
  3 KB (332 words) - 12:55, 10 January 2017
• Moyes 1989 J Exp Biol
  ...bolic fuels (fatty acids, pyruvate, amino acids but not ketone bodies), WM mitochondria appear to be specialized to use pyruvate. |preparations=Isolated mitochondria
  2 KB (298 words) - 09:34, 8 November 2016
• Calcium retention capacity
  ...x. By storing calcium in the form of osmotically inactive precipitates the mitochondria contribute to the buffering of cytosolic free Ca²⁺ levels and th ...been indirectly assessed by determination of respiratory rates of isolated mitochondria which were exposed to continuously increasing doses of Ca²⁺ by u
  1 KB (172 words) - 11:31, 22 April 2022
• Nascimento-Dos-Santos 2020 Biochim Biophys Acta Mol Basis Dis
  ...injury and modulation of oxidative metabolism by transplantation of active mitochondria to the retina. Biochim Biophys Acta Mol Basis Dis 1866:165686. ...c nerve crush. Our data show that intravitreally transplanted, full active mitochondria incorporate into the retina, improve its oxidative metabolism and electroph
  3 KB (345 words) - 10:00, 4 December 2020
• Labieniec 2011 Int J Mol Sci
  ...njection of streptozotocin (70 mg/kg body weight). Heart mitochondria were isolated from healthy rats and rats with streptozotocin-diabetes. Mitochondrial resp |preparations=Isolated mitochondria
  2 KB (303 words) - 11:22, 23 January 2019
• Sedlic 2010 Biochim Biophys Acta
  ...P)H-FP fluorometry is a useful method for exploring the effect of drugs on mitochondria and identifying their specific sites of action within the ETC of intact car |preparations=Isolated mitochondria, Intact cells
  2 KB (323 words) - 12:54, 14 February 2022
• Renner-Sattler Kathrin
  |area_of_interest=Isolated mitochondria, Cultured cells, Intact cells, Permeabilized cells, Permeabilized muscle fi |area_of_interest=Isolated mitochondria, Cultured cells, Intact cells, Permeabilized cells, Permeabilized muscle fi
  2 KB (273 words) - 18:57, 16 March 2020
• Krumschnabel 2016a Abstract MitoFit Science Camp 2016
  ...typically applied in high-resolution respirometry (HRR) comprise isolated mitochondria (Imt), tissue homogenates (Thom), and permeabilized tissue fibres (Pfi), ea |preparations=Permeabilized tissue, Homogenate, Isolated mitochondria
  2 KB (201 words) - 18:41, 10 January 2022
• Elustondo 2014 Biochim Biophys Acta
  swelling of isolated mitochondria, suggesting their role as inhibitors of the mitochondrial from the mitochondria detected with calcium green, 2) mitochondrial membrane
  2 KB (323 words) - 11:51, 27 October 2023
• Allen 2020 Thesis
  ...ives the advantage over other methods to enable the study of metabolism in mitochondria. |keywords=Mitochondria, Metabolism, Ischaemia-Reperfusion Injury
  2 KB (231 words) - 17:56, 14 May 2020
• Vaka 2017 Hypertension
  ...n and analysis. Respiration measurements were performed on intact isolated mitochondria under glutamate/malate as complex I substrate using Oroboros Oxygraph-2K. O ...8±0.09, p<0.05) and Complex II (0.7±0.03 vs 1±0.08, p<0.05) compared to NP mitochondria. State 3 (624±112 vs 878±9 pmol/sec/mg, p=0.22) and maximal (496±87vs 74
  2 KB (333 words) - 09:36, 25 September 2017
• Wyss 2016 Abstract IOC116
  ...Nieves Sanz M, Tevaearai Stahel HT, Longnus SL (2016) Tolerance of cardiac mitochondria to ischemia and reperfusion. Mitochondr Physiol Network 21.11 |preparations=Isolated mitochondria
  763 bytes (98 words) - 21:36, 2 December 2016
• Drahota 2012 Physiol Res
  ...ed mitochondrial swelling. These data thus indicate that the MPTP of heart mitochondria is better protected against damaging effects of the calcium load and oxidat |preparations=Isolated mitochondria
  2 KB (298 words) - 11:43, 24 February 2015
• Donnelly 2024 EU-METAHEART MC2 Meeting
  ...oso LHD, Kayser B, Place N, Gnaiger E (2024) Functional hypoxia in cardiac mitochondria: effects on oxidative phosphorylation, mitochondrial membrane potential, re ...chondria. Performing steady-state respirometry with isolated mouse cardiac mitochondria [2] we found that oxygen limitation of respiration reduced electron flow an
  2 KB (328 words) - 15:53, 13 March 2024
• Rasmussen 2004 Comp Biochem Physiol
  ...(2004) Skeletal muscle bioenergetics: a comparative study of mitochondria isolated from pigeon pectoralis, rat soleus, rat biceps brachii, pig biceps femoris ...that might effect simultaneous carbohydrate and fatty acid respiration. In mitochondria from the other muscles, the respiratory chain activity balanced the carbohy
  2 KB (336 words) - 17:57, 16 January 2021
• Intracellular oxygen
  ...t air saturation are effectively hyperoxic for cultured cells and isolated mitochondria.
  559 bytes (69 words) - 10:22, 6 October 2021
• Schilling 2017 J Mol Cell Cardiol
  mitochondria. comparing heart tissues and isolated mitochondria from wildtype (WT)
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• Rasmussen 1996 Biochem J
  ...HN, Andersen AJ, Fogd Jørgensen P, Quistorff B. (1996) Characterization of mitochondria from pig muscle: higher activity of exo-NADH oxidase in animals suffering f ...ctivities, including the nonphosphorylating exo-NADH oxidase activity. The mitochondria, prepared in a yield of 48%, showed high respiratory activities with tricar
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• Ferko 2014 Physiol Res
  ...: protective responses in functional and biophysical properties of cardiac mitochondria. Physiol Res 63:469-78. ...of RIP-induced protection against damage to the functions of mitochondria isolated by differential centrifugation after 30-min global ischemia followed by 40-
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• Hoene 2021 Mol Metab
  ...ke. We investigated how exercise alters the early compensatory response of mitochondria and thus prevents fatty liver disease as a long-term consequence of overnut ...tudy the alterations in the proteome, transcriptome and lipids in isolated mitochondria of liver and skeletal muscle as well as in whole tissue and examined the fu
  3 KB (427 words) - 17:18, 22 February 2022
• Valenzuela LR
  |topics=Parkinson disease, rat brain and cell culture isolated mitochondria, ROS production, Neuroprotection
  538 bytes (63 words) - 16:06, 24 July 2018
• Grimm 2016 Methods Mol Biol
  ...allmark proteins on the oxidative phosphorylation capacity of mitochondria isolated from the brain of transgenic AD mice. In the present chapter, we first intr |keywords=Mitochondria, Alzheimer’s disease, Amyloid-β, Tau, Oxygraph, High-resolution respirom
  2 KB (237 words) - 14:09, 22 August 2019
• Van den Broek 2010 FASEB J
  ...After 25 wk of HFD, maximal ADP-stimulated oxygen consumption in isolated mitochondria oxidizing pyruvate plus malate remained unaffected, while IMCL and mitochon |preparations=Isolated mitochondria
  2 KB (299 words) - 13:31, 24 June 2019
• Schoenfeld 2011 J Neurochem
  ...that PUFAs are likely to act as important extrinsic factor to enhance the mitochondria-associated intracellular ROS signaling in hypoxia. |keywords=Fatty acid, Hypoxia, Mitochondria, PC12 cells, Reactive oxygen species
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• Friederich-Persson 2012 PLoS One
  ...rambled siRNA. In conclusion, mitochondrial uncoupling via UCP-2 regulates mitochondria membrane potential in diabetes. However, blockade of the diabetes-induced u |preparations=Isolated mitochondria
  3 KB (345 words) - 16:35, 7 November 2016
• Borutaite 2015 Abstract MiPschool Cape Town 2015
  |title=Mitochondria and regulation of cell death during ischemia. from mitochondria, mitochondrial dysfunction, caspase activation and
  2 KB (318 words) - 08:23, 16 June 2015
• Tan 2011 Inflammation
  ...vitro'' mitochondrial function was measured after stimulation of isolated mitochondria of skeletal muscle cells with increasing doses of tert-BuOOH. tert-BuOOH in |preparations=Isolated mitochondria
  2 KB (278 words) - 14:26, 3 June 2015
• Malate-anaplerotic pathway control state
  ...at skeletal muscle mitochondria. However, many mammalian and non-mammalian mitochondria have a mt-isoform of NADP^+- or NAD(P)<big>+</big>-dependent [[ma ::::* DL-Protocol for isolated mitochondria and tissue homogenate (mt): [[SUIT-027 O2 ce-pce D065]]
  2 KB (283 words) - 01:28, 31 December 2020
• Klingenberg 1970 Eur J Biochem
  ...not permeate through the inner membrane to the matrix space in a number of mitochondria which actively oxidize glycerol-phosphate. # In mitochondria from various tissues ferricyanide can interact directly with glycerolphosph
  2 KB (204 words) - 14:55, 13 November 2017
• Monaco 2018 Am J Physiol Regul Integr Comp Physiol
  ...bsence of changes in cellular redox state and decreased the sensitivity of mitochondria to ADP (apparent K_m). These results add to the therapeutic poten |keywords=Bioenergetics, Heart, Hemodynamics, Mitochondria, Nitrate
  3 KB (371 words) - 09:26, 17 September 2018
• Mickevicius 2016 Thesis
  ...(2016) Effect of short time ischemia/reperfusion ''in vivo'' on rat kidney mitochondria oxidative phosphorylation. Diploma Thesis p38. ...ate an effect of short time ischemia/reperfusion ''in vivo'' on rat kidney mitochondria oxidative phosphorylation.
  2 KB (316 words) - 12:53, 13 November 2017
• Ramos MD
  |topics=fibers, isolated mitochondria, PBI-Shredder
  518 bytes (61 words) - 13:22, 19 August 2014
• Gross 2011 Anal Biochem
  ...GM, Stavrovskaya IG, Lazarev AV, Kristal BS (2011) Isolation of functional mitochondria from rat kidney and skeletal muscle without manual homogenization. Anal Bio ...sure pulses that can be adjusted to lyse cells and release organelles. The mitochondria subjected to pressure cycling-mediated tissue disruption are shown to retai
  3 KB (342 words) - 14:13, 21 July 2020
• Dawson 2022 FASEB J
  ...eases in respiratory capacity and O₂ affinity of subsarcolemmal mitochondria from skeletal muscle of high-altitude deer mice. https://doi.org/10.1096/fj ...itude mice. Our findings suggest that functional changes in subsarcolemmal mitochondria contribute to improving aerobic performance in hypoxia in high-altitude dee
  3 KB (340 words) - 17:44, 23 November 2022
• Trifunovic 2015 Fatty Acid Oxidation O2k-Network Discussion Forum
  |preparations=Isolated mitochondria
  500 bytes (59 words) - 10:25, 27 March 2018
• Volejnikova 2013 FEMS Yeast Res
  ...first 4 days, this fragmentation is associated with increasing function of mitochondria, while later on, it accompanies functional decline, which is also indicated |keywords=''Saccharomyces cerevisiae'', Chronological ageing, Mitochondria, Respiration
  2 KB (284 words) - 16:29, 13 November 2017
• Wenchich 2003 Physiol Res
  ...03) Polarographic evaluation of mitochondrial enzymes activity in isolated mitochondria and in permeabilized human muscle cells with inherited mitochondrial defect ...characterized, spectrophotometric measurements characterize activities of isolated complexes in disintegrated membranes. However, it may be concluded from our
  2 KB (315 words) - 15:46, 24 March 2015
• Arandarcikaite 2013 Abstract MiP2013
  ...tract=Our research group has previously reported that NO can protect heart mitochondria from ischemia-induced mitochondrial permeability transition pore-related re ...ypoxic chamber for 90 min. To test if the protective effect of NO on brain mitochondria during 90 min ischemia was due to PKG activation before inducing ischemia i
  3 KB (436 words) - 11:02, 28 April 2017
• Perry 2019 J Mol Cell Cardiol
  ...ub>2}/s*mg compared to 1816 ± 105 pmol O₂/s*mg in normoxic mitochondria) was attenuated with idebenone treatment (994 ± 76 vs pmol O_{2</su |keywords=Cardioprotection, Idebenone, Ischemia, Mitochondria, Reactive oxygen species, Reperfusion
  4 KB (533 words) - 13:21, 7 March 2020
• Herbst 2015 J Physiol
  ...the application of a transgenic mouse model overexpressing catalase within mitochondria. Through creating accessibility to small regions for the investigation of m |preparations=Permeabilized tissue, Isolated mitochondria
  2 KB (303 words) - 17:24, 7 November 2016
• Singh 2013 J Cereb Blood Flow Metab
  ...decrease in the respiratory control ratio ([[RCR]]) relative to noninjured mitochondria. In PZ-treated rats, RCR suppression was prevented (P<0.05 versus vehicle). |preparations=Isolated mitochondria
  2 KB (299 words) - 10:10, 8 November 2016
• Heim 2016 Thesis
  ...ation, substrate preference, and membrane composition of GMGS mitochondria isolated from liver, heart, skeletal muscle, and brown adipose tissue (BAT) from sum ...y. OXPHOS capacity was uniformly suppressed by decreasing Ta in all tissue mitochondria from torpid GMGS in the winter, despite widely variable changes in mitochon
  3 KB (442 words) - 13:54, 31 July 2017
• Felser 2013 Toxicol Sci
  ...uncoupled the respiratory chain. Furthermore, in acutely treated rat liver mitochondria and in HepG2 cells exposed for 24h, dronedarone started to inhibit mitochon ...ds=Dronedarone, Amiodarone, Hepatotoxicity, HepG2, Benzofuran derivatives, Mitochondria, Uncoupling, Electron transport chain, Apoptosis
  3 KB (363 words) - 16:28, 7 November 2016
• Kabiri 2021b Methods Mol Biol
  ...H (2021) Isolation and Electron Microscopic Analysis of Liver Cancer Cell Mitochondria. Methods Mol Biol 2277:277-287. ...iarities in disease settings that might not be evident in intact cells and allows for assessment of mitochondrial membrane integrity and sample purity.
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• Djafarzadeh 2017 J Vis Exp
  ...in biological samples (intact and permeabilized cells, tissues or isolated mitochondria). The high-resolution oxygraph device is equipped with two chambers and use |preparations=Isolated mitochondria
  2 KB (204 words) - 16:28, 27 March 2018
• Rundle 2018 Environ Sci Technol
  ...c acids are oxidative uncouplers and impair electron transport in isolated mitochondria. Environ Sci Technol 52:10803-11. ...h 90% fitting the classical O₂-NA definition. Mitochondria were isolated from rainbow trout liver and exposed to different concentrations of NAs. Mi
  2 KB (319 words) - 15:33, 2 January 2019
• Drahota 2014 Physiol Res
  ...va Z, Cahova M (2014) Biguanides inhibit Complex I, II and IV of rat liver mitochondria and modify their functional properties. Physiol Res 63:1-11. ...ly mitochondrial Complex I but also Complex II and IV activity in isolated mitochondria. The enzymes complexes inhibition is paralleled by the decreased respirator
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• CA Montreal Gouspillou G
  |keyword=Aging, exercise, nutrition, permeabilized muscle fibers, isolated mitochondria
  547 bytes (74 words) - 15:13, 26 July 2023
• Konrad 2012 PLoS One
  ...mations of electron-dense material strikingly similar to those observed in mitochondria from the hepatopancreas of blue crab (''Callinectes sapidus'') and the embr |preparations=Isolated mitochondria
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• Hogeboom 1948 J Biol Chem
  ...tion of intact mitochondria from rat liver; some biochemical properties of mitochondria and submicroscopic particulate material. J Biol Chem 172:619-35. |keywords=Liver, Mitochondria
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• Tahara 2009 Free Radic Biol Med
  ...es. Our results also indicate that succinate is an important substrate for isolated mitochondrial reactive oxygen production in brain, heart, kidney, and skele |preparations=Isolated mitochondria
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• Simonin 2012 Biochem J
  ...inhibits succinate dehydrogenase-driven oxygen consumption in potato tuber mitochondria in an oxygen tension-independent manner. Biochem J 449:263-73. ...These data indicate that SDH has an alternative site of NO action in plant mitochondria.
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• Hepple 2012 Abstract Bioblast
  ...ng skeletal muscle, our results also suggest that vulnerabilities in aging mitochondria from skeletal muscle may be at least partially compensated for by the mitoc ...Mitochondrial functional impairment with aging is exaggerated in isolated mitochondria compared to permeabilized myofibers. Aging Cell 9: 1032-1046.]
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• Jackman 1996 Am J Physiol Cell Physiol
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• Kussmaul 2006 Proc Natl Acad Sci
  ...production by NADH:ubiquinone oxidoreductase (complex I) from bovine heart mitochondria. Proc Natl Acad Sci 103:7607-12. ...t clearly links our mechanism for the isolated enzyme to studies on intact mitochondria, in which superoxide production is enhanced when the NAD+ pool is reduced.
  2 KB (296 words) - 21:40, 13 April 2022
• Donnelly 2024 Redox Biol
  ...and exercising humans. Performing steady-state respirometry with isolated mitochondria we found that oxygen limitation of respiration reduced electron flow and ox |preparations=Intact organism, Isolated mitochondria, Intact cells
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• Dahdah 2019 MiP2019
  ...e and the loss of damaged mitochondria through the isolation process. With isolated adipocytes, the protocol is time consuming and might lead to the lysis and ...ochondrial respiration in mouse epididymal WAT homogenates and adipocytes (isolated from the same tissue) and by measuring mitochondrial respiration under diff
  3 KB (451 words) - 14:39, 3 October 2019
• Fink 2019 FASEB J
  ...on of complex II-energized respiration in muscle, heart, and brown adipose mitochondria by oxaloacetate and complex I electron flow. FASEB J 33:11696-705. ...not high ΔΨ (heart). In summary, complex II-energized respiration in IBAT mitochondria is tempered by complex I-derived OAA in a manner dependent on UCP1. These d
  2 KB (317 words) - 01:37, 3 April 2023
• Chance 1961 J Biol Chem-I
  ...nger G (1961) The interaction of energy and electron transfer reactions in mitochondria I. General properties and nature of the products of succinate-linked reduct ...idine nucleotide caused by the addition of succinate to isolated mitochondria has been demonstrated. The material so reduced exhibits kinetic r
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• Meza-Buendia 2022 PLoS One
  ...gado C, Paschke K, Rosas C (2022) High resolution respirometry of isolated mitochondria from adult ''Octopus maya'' (Class: Cephalopoda) systemic heart. https://do ...ed in this paper takes approximately 3 to 4 hours from isolation of intact mitochondria to measurement of mitochondrial oxygen consumption.
  2 KB (312 words) - 13:36, 5 September 2022
• Davis 2024 BEC
  ...that Magnesium Green has no significant effect on respiration of isolated mitochondria, whereas tetramethylrhodamine inhibits phosphorylating respiration and incr |preparations=Isolated mitochondria
  2 KB (269 words) - 15:49, 3 May 2024
• ElBacha 2010 Abstract IOC60
  ...le=El-Bacha T, Abud M, Galina A, Da Poian AT (2010) Bioenergetics of liver mitochondria from mice infected with Dengue virus. MiPNet15.10. ...increase in both respiratory control ratio of complex I and complex II in mitochondria from DenV-infected animals when compared to controls. These early alteratio
  3 KB (440 words) - 17:07, 27 March 2018
• Parrino 2000 J Exp Biol
  ...DW, Doeller JE (2000) ATP production from the oxidation of sulfide in gill mitochondria of the ribbed mussel ''Geukensia demissa''. J Exp Biol 203:2209-18. ...nment by oxidizing sulfide in gill mitochondria. Well-coupled mitochondria isolated from G. demissa gills were used to investigate sulfide oxidation and ATP sy
  2 KB (305 words) - 10:08, 9 November 2016
• Devin 1997 Biochim Biophys Acta
  ...rin B, Rigoulet M (1997) Control of oxidative phosphorylation in rat liver mitochondria: effect of ionic media. Biochim Biophys Acta 1319:293-300. ...d: for inorganic cations, these K0.5 values increased, as did the absolute value of hydratation enthalpy. The ATP/O ratio did not vary in any medium and mat
  2 KB (285 words) - 13:52, 15 January 2020
• Murphy 2009 Biochem J
  |title=Murphy MP (2009) How mitochondria produce reactive oxygen species. Biochem J 417:1-13. ...a ''in vivo'' from O₂^•−-production rates by isolated mitochondria, and such extrapolations in the literature are misleading. Even so, the des
  6 KB (883 words) - 11:59, 20 December 2022
• Holzem 2016 FASEB J
  ...opy images also showed no ultrastructural remodeling for failing vs. donor mitochondria; however, the fraction of lipid droplets (LDs) in direct contact with a mit |preparations=Permeabilized tissue, Isolated mitochondria
  2 KB (316 words) - 14:31, 27 November 2017
• Meszaros 2014 Abstract MiP2014
  ...al mitochondrial studies targets many tissues and organs, small intestinal mitochondria are less frequently examined. Nevertheless, the integrity of small intestin ...rential centrifugation. The assessment of the functional state of isolated mitochondria was performed by means of high-resolution respirometry (Oroboros Oxygraph-2
  3 KB (471 words) - 12:44, 23 January 2019
• FNSGp
  ::::* DL-Protocol for isolated mitochondria and tissue homogenate (mt): [[SUIT-002 O2 mt D005]] ::::* DL-Protocol for isolated mitochondria and tissue homogenate (mt): [[SUIT-002 O2 mt D005]]
  2 KB (207 words) - 04:54, 19 July 2022
• Christiansen 2015 Am J Physiol Heart Circ Physiol
  ...HCM and support the hypothesis that restoration of the redox-state in the mitochondria is beneficial in the treatment of HCM. |keywords=Hypertrophic cardiomyopathy, Mitochondria, Oxidative stress, Amplex Red
  3 KB (373 words) - 17:34, 25 February 2020
• Nedergaard 2017 MiP2017
  |title=[[Image:NedergaardJ.jpg|left|90px|Jan Nedergaard]] Creatine effect in mitochondria from different tissues. ...the present study, we compared the effect of creatine addition to isolated mitochondria from heart, liver, brown and brite/beige adipose tissue.
  4 KB (531 words) - 17:56, 22 November 2017
• Naszai 2019 Sci Rep
  ...delay in Ca²⁺ movement was observed, not only in isolated liver mitochondria, but also in tissue homogenates. In all samples, the transition to anoxia r |preparations=Isolated mitochondria
  2 KB (347 words) - 11:51, 27 October 2023
• Skalska 2009 Int J Mol Sci
  ...n AP, Kunz WS, Szewczyk A (2009) Calcium ions regulate K uptake into brain mitochondria: The evidence for a novel potassium channel. Int J Mol Sci 10:1104-20. ...rmed the predominant occurrence of anti-β₄ subunit in neuronal mitochondria. We hypothesize that the mitochondrial BKCa channel represents a calcium se
  2 KB (332 words) - 14:51, 27 March 2018
• Liver mitochondria purification
  |preparations=Isolated Mitochondria
  595 bytes (68 words) - 22:41, 27 January 2016
• Power 2014 Physiol Rep
  ...rdiac energy demands, while between this and critical hyperthermia (43°C), mitochondria become net consumers of ATP. This consumption may contribute to cardiac fai |preparations=Isolated mitochondria
  3 KB (365 words) - 15:40, 17 January 2023
• Figueira 2014 Abstract MiP2014
  ...tissues: conversely from skeletal muscle, heart and brain, isolated liver mitochondria exhibit gender dimorphism in respiratory activity. |abstract=Previous data provided evidence that isolated liver mitochondria from female rats present higher rates of resting ([[LEAK]]) and ADP-stimula
  4 KB (486 words) - 12:09, 24 February 2015
• Lewis 2022 Methods Mol Biol
  ...P sensitivity. We propose this function defines the bioenergetic role that mitochondria fill within the myoplasm to sense and match ATP demands. Any deficit in thi |preparations=Intact organism, Isolated mitochondria
  2 KB (253 words) - 11:05, 18 December 2022
• Jezek 2010 Physiol Res
  ...phospholipase A₂ activated by reactive oxygen species in heart mitochondria induces mild uncoupling. Physiol Res 59:737-47. ...2}) is activated by ''tert''-butylhydroperoxide in isolated rat heart mitochondria. Isoform specificity was judged from the inhibition by bromoenol lactone (B
  2 KB (297 words) - 15:25, 26 May 2015
• Kancirova 2016 Physiol Res
  ...ia/reperfusion test according to Langendorff. Thereafter mitochondria were isolated and the mitochondrial respiration was measured. Additionally, the ATP synth |keywords=Myocardial preconditioning, Mitochondria, Energy metabolism, Mitochondrial ATP synthase, Myocardial ischemic reperfu
  2 KB (320 words) - 16:44, 26 July 2017
• Hoffman 2007 Am J Physiol Heart Circ Physiol
  ...ial ROS generation to defined steady-state [O(2)] as low as 0.1 microM. In mitochondria respiring under state 4 (quiescent) or state 3 (ATP turnover) conditions, d |preparations=Isolated mitochondria
  2 KB (301 words) - 21:38, 13 April 2022
• Littlejohns 2014 PLoS One
  ...ogenic high-fat diet exhibit changes in their oxidative state, calcium and mitochondria in parallel with increased susceptibility to reperfusion injury. PLoS One 9 ...th higher diastolic intracellular Ca2+ concentration and greater damage to isolated cardiomyocytes following simulated ischemia/reperfusion. High-fat diet was
  3 KB (366 words) - 09:17, 8 November 2016
• Leung 1975 J Biol Chem
  ...n transfer. The results indicate that energy coupling can be observed with isolated reduced coenzyme Q-cytochrome c reductase if the enzyme complex is properly |preparations=Isolated mitochondria
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• Andrienko 2003 J Exp Biol
  ...reiter R, Saks VA (2003) Metabolic consequences of functional complexes of mitochondria, myofibrils and sarcoplasmic reticulum in muscle cells. J Exp Biol 206:2059 ...taken to show strong structural associations (functional complexes) among mitochondria, sarcomeres and sarcoplasmic reticulum. Inside these complexes, mitochondri
  3 KB (405 words) - 15:30, 24 March 2015
• Paech 2018b Toxicology
  ...conclusion, sunitinib caused concentration-dependent toxicity in isolated mitochondria at concentrations reached in livers ''in vivo'' and inhibited hepatic mitoc |preparations=Isolated mitochondria
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• Hansson 2010 J Biol Chem
  ...asson G, Uchino H, Elmer E (2010) Increased potassium conductance of brain mitochondria induces resistance to permeability transition by enhancing matrix volume. J ...ting respiration. We propose that increased potassium conductance in brain mitochondria may cause a direct physiological effect on matrix volume inducing resistanc
  3 KB (388 words) - 17:34, 25 February 2020
• Gyamfi 2012 Free Radic Biol Med
  ...with or without 100 mM ethanol. The results showed that in isolated liver mitochondria both palmitic and arachidonic acids significantly reduced state 3 respirati |preparations=Isolated mitochondria, Intact cells
  3 KB (358 words) - 12:29, 12 July 2023
• Sokolov 2018 Mitochondrion
  ...lity of taurine with the metabolic functions and its beneficial effects on mitochondria may have contributed to its broad distribution as an osmolyte in marine osm |preparations=Isolated mitochondria
  2 KB (324 words) - 13:01, 17 April 2019
• Aguirre 2012 Mitochondrion
  ...(2012) Functional evidence for nitric oxide production by skeletal-muscle mitochondria from lipopolysaccharide-treated mice. Mitochondrion 12:126-31. ...lipopolysaccharide (LPS). In the presence of the NOS substrate L-arginine, mitochondria from LPS-treated mice had lower respiration rates and higher P(50) values t
  1 KB (197 words) - 15:43, 10 March 2015
• Jensen 2021 Free Radic Biol Med
  ...om torpid 13-lined ground squirrels, but has a weak effect on mitochondria isolated during summer and hibernation arousals, where metabolic rate is normal. Con |preparations=Isolated mitochondria
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• Kharechkina 2019 Biochim Biophys Acta Gen Subj
  ...A, Kruglov AG (2019) Regulation of permeability transition pore opening in mitochondria by external NAD(H). Biochim Biophys Acta Gen Subj 1863:771-83. |abstract=The opening of the permeability transition pore (PTP) in mitochondria plays a critical role in the pathogenesis of numerous diseases. Mitochondri
  3 KB (382 words) - 12:10, 26 July 2019
• Larsen 2020 Acta Physiol (Oxf)
  In this study, we examined the p50_mito of mitochondria isolated from the ''Vastus lateralis'' and ''Triceps brachii'' in 12 healthy volunte We here show that p50_mito is similar in isolated mitochondria from the ''Vastus'' (40 ± 3.8 Pa) compared to ''Triceps'' (39 ± 3.3) but
  3 KB (390 words) - 23:20, 15 July 2022
• Chance 1961 J Biol Chem-III
  ...ochondria III. Substrate requirements for pyridine nucleotide reduction in mitochondria. J Biol Chem 236:1555-61. ...pyridine nucleotide is demonstrated in pigeon heart and guinea pig kidney mitochondria.
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• Ashok 2023 Mol Cell Biochem
  ...e observed effect could be an outcome of the accumulation of dysfunctional mitochondria due to autophagy inhibition. |keywords=Chloroquine, Heart, High-resolution respirometry, Metabolism, Mitochondria
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• Deline 2021 Biochim Biophys Acta Bioenerg
  ...late kinase derived ATP shapes respiration and calcium storage of isolated mitochondria. Biochim Biophys Acta Bioenerg 1862:148409. ...nase, Amorphous calcium phosphate, Calcium capacity, Cytochrome c oxidase, Mitochondria, P/O ratio
  2 KB (259 words) - 11:12, 8 July 2021
• SUIT-018
  ...ence of O₂ flux and H₂O₂ flux in isolated mitochondria,tissue homogenate or permeabilized cells''' ...ence of O₂ flux and H₂O₂ flux in isolated mitochondria, tissue homogenate (except of liver homogenate) or permeabilized cells. In
  5 KB (778 words) - 23:47, 12 April 2021
• Kalinovich 2015 Abstract MiP2015
  ...pacity (amount of UCP1 protein by Western blot and respiration of isolated mitochondria), as well as lipid metabolism (expression of genes involved in lipid synthe ...ent) upregulated in the UCP3 KO mice. The thermogenic function of isolated mitochondria did not differ between wild-type and UCP3 KO mice.
  3 KB (400 words) - 10:58, 4 September 2015
• Chowdhury 2015 Biosci Rep
  ...ates and mitochondrial membrane potential by TMRM and safranin in cortical mitochondria. Biosci Rep 36:e00286. ...ultaneous evaluation of mtMP and respiratory chain activity using isolated mitochondria and tissue homogenate. However, certain care should be taken concerning the
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• Parsons 1966 Ann N Y Acad Sci
  ...of isolated and purified preparations of the outer and inner membranes of mitochondria. Ann N Y Acad Sci 137: 643-666.
  457 bytes (62 words) - 12:49, 20 June 2012
• Labajova 2006 Anal Biochem
  ...tested our improved device for membrane potential measurements of isolated mitochondria (in absolute scale of millivolts). In addition, we assessed relative change |keywords=Membrane potential, TPP-selective electrode, Mitochondria, Hepatocytes, Cultured cells
  2 KB (259 words) - 11:16, 18 March 2020
• Donnelly 2023 MitoFit
  ...and exercising humans. Performing steady-state respirometry with isolated mitochondria we found that oxygen limitation of respiration reduced electron flow and ox |preparations=Isolated mitochondria
  3 KB (469 words) - 09:13, 27 March 2024
• Fuhrmann 2018 Cell Mol Life Sci
  ...trate-specific mitochondrial respiration. Complexome profiling of isolated mitochondria of the human leukemia monocytic cell line THP-1 revealed HIF-1α-dependent |preparations=Isolated mitochondria, Intact cells
  2 KB (296 words) - 18:31, 31 January 2020
• Renner 2016 Mycotoxin Res
  ...PS stress. The different function was not linked to modifications of liver mitochondria. |keywords=Deoxynivalenol, Lipopolysaccharide, Liver, Mitochondria, Pig
  2 KB (356 words) - 09:51, 27 June 2019
• Maekawa 2019 Biochem Biophys Rep
  ...I is essential for the normal functioning of complex I and II in mouse SKM mitochondria. |preparations=Permeabilized tissue, Isolated mitochondria
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• Liepinsh 2016 Biochem J
  ...ain acylcarnitines determine ischaemia/reperfusion-induced damage in heart mitochondria. Biochem J 473:1191-202. ...ylcarnitines are harmful FA intermediates, accumulating in ischaemic heart mitochondria and inducing inhibition of oxidative phosphorylation. Therefore, decreasing
  3 KB (359 words) - 13:40, 7 March 2020
• Palmeira 2019 MiP2019
  ...vention. As such, a pharmacological intervention that could mimic surgical mitochondria precondition could improve mitochondrial function in a setting of rodent mo ...tion with LRE1 previous to surgical IR. After 24h, liver mitochondria were isolated and several metabolic parameter assessment tests were conducted.
  2 KB (344 words) - 16:01, 7 October 2019
• Macedo 2020 FASEB J
  ...itochondria, whereas both interventions enhanced the efficiency with which mitochondria utilized lipids as respiratory substrates. Notably, the combination of BD a |preparations=Intact organism, Isolated mitochondria
  3 KB (364 words) - 16:19, 2 December 2021
• OctPGMS pathway control state
  ::::* DL-Protocol for isolated mitochondria and tissue homogenate (mt): [[SUIT-002 O2 mt D005]] ::::* DL-Protocol for isolated mitochondria and tissue homogenate (mt): [[SUIT-001 O2 mt D001]]
  2 KB (209 words) - 05:01, 19 July 2022
• Kang 2018 Exp Mol Med
  ...im KS, Song MY, Park KS, Kim YH, Pak YK (2018) Cell-penetrating artificial mitochondria-targeting peptide-conjugated metallothionein 1A alleviates mitochondrial da ...we predict that CAMP could be used to deliver other cargo proteins to the mitochondria.
  2 KB (268 words) - 17:23, 25 February 2020
• Hoeks 2012 J Cell Physiol
  ....04). Uncoupling protein 3 (UCP3) levels were markedly reduced in PGC-1αTg mitochondria (''p'' < 0.001). Taken together, in addition to stimulating mitochondri |keywords=Skeletal muscle, Mitochondria, Fat metabolism, ROS, Mitochondrial uncoupling
  3 KB (355 words) - 14:38, 13 November 2017
• Logan 2018 Thesis
  ...d analyzed oxygen consumption using high resolution respirometry. Isolated mitochondria, exposed to various substrates of respiration exhibited no change in oxygen |preparations=Isolated mitochondria
  3 KB (368 words) - 13:31, 7 March 2020
• Krajcova 2020 PLOS ONE
  ...korná E, Duška F (2020) High resolution respirometry to assess function of mitochondria in native homogenates of human heart muscle. PLOS ONE 15:e0226142. https:// ...≤8% for all indices. We found that whereas atrial myocardium contains less mitochondria than the ventricle, atrial bioenergetic profiles are comparable to left ven
  3 KB (373 words) - 03:31, 18 April 2024
• Menezes-Filho 2017 Free Radic Biol Med
  ...cordingly, both depletion of mitochondrial adenine nucleotides and loading mitochondria with exogenous ATP abolished the differences between CR and ad libitum (AL) |keywords=ATP, Ca(2+), Calorie restriction, Ischemic protection, Liver, Mitochondria
  2 KB (315 words) - 15:18, 13 November 2017
• Komlodi 2016 Abstract IOC116
  ...eactive oxygen species production - A comparative study using mitochondria isolated from different mammalian tissues. Mitochondr Physiol Network 21.11 ...upported ROS production on the Δ''Ψ''_mt and ΔpH in mitochondria isolated from guinea-pig brain, heart and skeletal muscle. Consequently, we examined
  3 KB (515 words) - 13:06, 7 March 2020
• Schwerzmann 1986 J Cell Biol
  ...Sänger A, Weibel ER (1986) Molecular architecture of the inner membrane of mitochondria from rat liver: a combined biochemical and stereological study. J Cell Biol ...l and biochemical data, it was estimated that the individual mitochondrion isolated from rat liver has a volume of 0.27 µm³, an inner membrane area
  2 KB (322 words) - 18:00, 16 January 2021
• Chen 2018 Endocrinology
  ...uppressed pyruvate metabolism in isolated mitochondria. Using mitochondria isolated from brown adipose tissue in mice with adipocyte-specific deletion of the M |preparations=Isolated mitochondria
  2 KB (316 words) - 16:04, 16 February 2021
• Yu 2013 Diabetes
  ...ew mechanistic insight into the dysregulation of ATP production and ROS in mitochondria of insulin-deficient rodents. |preparations=Isolated mitochondria
  2 KB (296 words) - 14:24, 28 March 2018
• Brown 2014 J Cardiovasc Pharmacol Ther
  ...Neufer PD, Kloner RA (2014) Reduction of early reperfusion injury with the mitochondria-targeting peptide bendavia. J Cardiovasc Pharmacol Ther 19:121-32. ...generation (as opposed to augmented scavenging) by endothelial and myocyte mitochondria.
  3 KB (428 words) - 04:38, 12 February 2020
• Schoenfeld 2009 Free Radic Biol Med
  ...ascribed to contaminating peroxisomes. Preparations of rat brain and heart mitochondria were not or were much less contaminated with peroxisomes, as indicated by l |keywords=Reactive oxygen species, Mitochondria, Peroxisomes, Acyl-CoA, Palmitoyl-CoA, Free radicals
  2 KB (286 words) - 19:02, 16 January 2021
• Kauppinen 1984 Am J Physiol
  ...n RA, Hassinen IE (1984) Monitoring of mitochondrial membrane potential in isolated perfused rat heart. Am J Physiol 247:H508-16. ...e mitochondria. A decrease of the fluorescence was found on energizing the mitochondria by decreasing the cellular energy consumption by arrest induced by 18 mM K+
  2 KB (286 words) - 09:33, 4 April 2022
• Belosludtsev 2018 Biochemistry (Mosc)
  ...equalinium on respiration and the inner membrane permeability of rat liver mitochondria. Biochemistry (Mosc) 12:121–27. ...e rate of the H₂O₂ production increased in rat liver mitochondria. Possible mechanisms of toxic effect of dequalinium chloride are discussed.
  2 KB (282 words) - 09:04, 5 October 2018
• Schnaitman 1967 J Cell Biol
  ...monoamine oxidase. An enzymatic marker for the outer membrane of rat liver mitochondria. J Cell Biol 32:719-35. ...f the cytochrome oxidase and malate dehydrogenase activities of the intact mitochondria, while the monoamine oxidase activity could be recovered in the form of sma
  2 KB (288 words) - 12:11, 28 May 2015
• Chicco 2016a Abstract MitoFit Science Camp 2016
  ...nce of H₂O₂ release from skeletal muscle and cardiac mitochondria: influence of sample preparation. ...lux during Complex I+II-linked ADP-phosphorylating respiration of isolated mitochondria, saponin-permeabilized fibers, and tissue homogenates obtained from skeleta
  4 KB (631 words) - 13:06, 7 March 2020
• Gellerich 2012 Biochem J
  ...12) Cytosolic Ca²⁺ regulates the energisation of isolated brain mitochondria by formation of pyruvate through the malate-aspartate shuttle. Biochem J 44 |abstract=The glutamate-dependent respiration of isolated brain mitochondria (BM) is regulated by cytosolic Ca2+ (Ca2+cyt) (S0.5 = 225 ± 22 nM) through
  2 KB (320 words) - 10:46, 13 March 2015
• Endlicher 2009 Acta Medica (Hradec Kralove)
  ...eart mitochondria are more resistant to damaging effect of Ca2+ than liver mitochondria. These finding thus support hypothesis that there exist tissue specific str |keywords=Mitochondria, MPTP, Swelling, Cell death, Apoptosis, Necrosis
  2 KB (234 words) - 11:08, 18 March 2020
• Veksler 1987 Biochim Biophys Acta
  ...and isopreterenol treatment were found to affect respiratory parameters of mitochondria in saponin-skinned fibers, among them creatine-stimulated respiration decre
  1 KB (174 words) - 11:49, 27 February 2013
• Jimenez-Aranda 2014 J Pineal Res
  ...lso caused a profound inhibition of Ca-induced opening of mPTP in isolated mitochondria from both types of fat, white and beige, in both lean and obese rats. These |preparations=Isolated mitochondria
  3 KB (400 words) - 08:04, 21 February 2020
• Hanley 2005 J Physiol
  ...i JJ, Van Veldhoven PP, Daut J (2005) 5-Hydroxydecanoate is metabolised in mitochondria and creates a rate-limiting bottleneck for beta-oxidation of fatty acids. J ...oA or lauryl-carnitine. In conclusion, 5-HD is activated, transported into mitochondria and metabolised via β-oxidation, albeit with rate-limiting kinetics at the
  3 KB (365 words) - 17:05, 19 February 2018
• PM-pathway control state
  ::::* DL-Protocol for isolated mitochondria and tissue homogenate (mt): [[SUIT-001 O2 mt D001]] ::::* DL-Protocol for isolated mitochondria and tissue homogenate (mt): [[SUIT-006 O2 mt D047]]
  5 KB (603 words) - 15:33, 2 August 2023
• Ragan 1975 J Biol Chem
  ...nd K+ ions. The results indicate that energy coupling can be observed with isolated NADH-coenzyme Q reductase if the enzyme complex is properly incorporated in |preparations=Isolated mitochondria
  2 KB (235 words) - 10:11, 26 February 2015
• Petrus 2015 Can J Physiol Pharmacol
  ...he mitochondrial H₂O₂ release. In isolated rat heart mitochondria, the novel benzopyran analogues act as protonophoric uncouplers of oxidativ |keywords=Rat heart mitochondria, Benzopyran analogues, Protonophores, Uncoupling, Hydrogen peroxide
  3 KB (345 words) - 13:03, 7 March 2020
• Kancirova 2016 Nove Strategicke Pristupy v experimentalnej Kardioprotekcii
  ...genous protection. The aim of our study was to elucidate the role of heart mitochondria as the final effector of noninvasive form of remote ischemic ...(200 mmHg) of the right hind limb using a pressure cuff. Mitochondria were isolated by differential centrifugation using
  4 KB (515 words) - 13:22, 23 January 2019
• Sivaramakrishnan 1975 Environ Physiol Biochem
  ...n S, Ramasarma T (1975) Oxidation of succinate in heart, brain, and kidney mitochondria in hypobaria and hypoxia. Environ Physiol Biochem 5:189-200. ...ive species. Succinate-NT reductase of the heart, the brain and the kidney mitochondria showed a high activation with ubiquinone whereas its reduced form had no st
  2 KB (287 words) - 14:55, 1 December 2015
• Hoppel 2017 MiPschool Obergurgl E1
  ...phospholipid and supercomplex analysis for a comprehensive examination of mitochondria. The challenge in 2017 is that the use functional testing has declined as g |preparations=Isolated mitochondria
  2 KB (312 words) - 14:54, 28 March 2018
• Horvath 2022 Antioxidants (Basel)
  ...b> formation supported by succinate or α-GP were inhibited in mitochondria isolated from the transgenic animals. The decrease of RET-evoked ROS production by D ...RET, ROS, Antioxidant systems, Cellular respiration, Ischemia-reperfusion, Mitochondria, Oxoglutarate dehydrogenase complex, Reactive oxygen species, Reverse elect
  3 KB (448 words) - 15:57, 15 December 2022
• Gear 1970 Biochem J
  |title=Gear AR (1970) Inner- and outer-membrane enzymes of mitochondria during liver regeneration. Biochem J 120:577-87. ...ane, inter-membrane space and outer membrane were measured in mitochondria isolated from control and regenerating rat liver. The specific activity of these enz
  3 KB (394 words) - 10:35, 13 February 2015
• Zheleznova 2012 Physiol Genomics
  ...n and high resolution respirometry analyses showed that mTAL cells and the mitochondria in the outer medulla of SS rats fed high salt diet exhibited lower rates of |preparations=Isolated mitochondria
  2 KB (315 words) - 14:42, 13 February 2015
• Scott 2013 Abstract MiP2013
  ...oxygen kinetics, and several other variables were measured in mitochondria isolated from the flight muscle of bar-headed geese and the hindlimb muscle of highl |preparations=Isolated mitochondria, Enzyme
  2 KB (222 words) - 11:23, 28 April 2017
• Muellebner 2015 Biomolecules
  ...Kozlov AV, Duvigneau JC (2015) Heme degradation by heme oxygenase protects mitochondria but induces ER stress via formed bilirubin. Biomolecules 5:679-701. ...mitochondria respond differently to elevated levels of BR and HO-activity. Mitochondria are susceptible to hemin, but active HO protects them against hemin-induced
  3 KB (391 words) - 12:12, 8 November 2016
• Steffen 2023 J Exp Biol
  ...gher respiration. The high plasticity and stress tolerance of ''C. gigas'' mitochondria may contribute to the success of this invasive species and facilitate its f ...obic capacity, AmplexRed, High-resolution respirometry, Hypoxia tolerance, Mitochondria, Oxidative stress marker
  3 KB (379 words) - 18:14, 25 July 2023
• Endlicher 2021 Physiol Res
  ...nction of mitochondrial membrane permeability transition pore in rat liver mitochondria. Physiol Res 70:905-11. ...changes were observed while testing calcium-induced swelling of rat liver mitochondria. These findings indicate that the mitochondrial energy production system is
  2 KB (231 words) - 16:54, 25 January 2022
• Rustenbeck 1998 Biochem Pharmacol
  ...yamine-analogues-on-mitochondrial-calcium-uptake/?from_term=Spermidine+and+mitochondria&from_page=10&from_pos=8 PMID: 9776308] ...2+ uniporter. The ability of aminoglucosides to displace spermine from the mitochondria and to inhibit mitochondrial Ca2+ accumulation may contribute to the mitoch
  3 KB (373 words) - 14:30, 19 February 2020
• Brown 2015 Abstract MiPschool Cape Town 2015
  ...ochondrial function in isolated hearts, permeabilized fibers, and isolated mitochondria. We hypothesized that this peptide would improve mitochondrial membrane flu ...uidity decreased sharply at the onset of reoxygenation (32,335 ± 3948 AU). Mitochondria treated with MTP-131 displayed higher membrane fluidity in early reperfusio
  3 KB (444 words) - 13:13, 23 January 2019
• Afshordel 2014 Prostaglandins Leukot Essent Fatty Acids
  ...reased the levels of anti-apoptotic Bcl-2 protein. Cell membrane fractions isolated from the brain of aged mice exhibited lower membrane fluidity, which was pa |preparations=Isolated mitochondria
  3 KB (381 words) - 10:49, 27 March 2018
• Jayakumari 2020 J Diabetes
  ...a known fact that permeabilized cardiac muscle fibres as well as isolated mitochondria are metabolically compromised in Caucasian population, studies in Asian Ind ...te utilization was measured using high-resolution respirometry in isolated mitochondria prepared from right atrial appendage tissues of diabetic and non-diabetic s
  3 KB (381 words) - 16:54, 1 July 2020
• Serna 2022 BEC
  ...owski AJ (2022) Measuring mitochondrial Ca²⁺ efflux in isolated mitochondria and permeabilized cells. Bioenerg Commun 2022.7. https://doi.org/10.26124/b ...and insensitive mitochondrial Ca²⁺ efflux activity in isolated mitochondria and permeabilized cells using the Ca²⁺ Green indicator and a flu
  3 KB (476 words) - 16:41, 21 January 2024
• Larsen FJ 2013 Abstract MiP2013
  ...ochondria has a unique response to ischemia-reperfusion injury compared to mitochondria from rat, mouse and pig . Mitochondr Physiol Network 18.08. ...pharmacological approaches to attenuate ischemic injury [2]. Mice and rat mitochondria are by far the most common animal model in this area of research.
  3 KB (372 words) - 16:23, 27 March 2018
• Pichova A
  ...rather lysate – within the study of stress and drug, diseases influence on mitochondria in intestinal system and marginally in another tissues from mouse (conventi
  1 KB (176 words) - 15:40, 6 September 2022
• Malinska 2010 Biochim Biophys Acta
  ...zyk A, Kunz WS (2010) Complex III-dependent superoxide production of brain mitochondria contributes to seizure-related ROS formation. Biochim Biophys Acta 1797:116 ...ns and uncouplers, mimickingmitochondrial activation, on ROS generation of isolated rat andmouse brainmitochondria. Since these stimulatory effectswere visible
  2 KB (296 words) - 16:43, 19 March 2015
• Ravasz 2017 Neurochem Int
  ...ted by either vigabatrin or aminooxyacetic acid. It is concluded that when mitochondria catabolize GABA or succinic semialdehyde or GHB through the GABA shunt, the |preparations=Isolated mitochondria
  3 KB (357 words) - 08:16, 28 March 2018
• Kagawa 1971 J Biol Chem
  ...epleted in P-lipids and cytochrome oxidase were isolated from bovine heart mitochondria and were reconstituted with P-lipids and coupling factors to yield vesicula |preparations=Isolated mitochondria
  2 KB (236 words) - 13:00, 24 February 2015
• Juhaszova 2019 bioRxiv
  ...nt agreement between F₁F_o single-molecule and intact-mitochondria experiments is consistent with K⁺-transport through ATP synthase
  2 KB (330 words) - 06:38, 14 November 2023
• Maddalena 2014 Abstract MiP2014
  |title=Experimental considerations for evaluating bioenergetic effects of a mitochondria-targeted compound, TPP-IOA. ...re evaluating the dose-effect relationships of TPP-IOA’s interactions with mitochondria.
  3 KB (394 words) - 16:58, 24 February 2015
• Saks 2003 Biophys J
  ...-PEP in intact permeabilized fibers, but some part of it was released from mitochondria after short proteolysis due to increased permeability of outer mitochondria |preparations=Intact cells, Permeabilized tissue, Isolated mitochondria
  2 KB (308 words) - 15:41, 24 March 2015
• Bryant 2018 Thesis
  ...stingly, I also observed increases in proton conductance in isolated liver mitochondria from cold-acclimated northern killifish as indicated by increased O<sub>2</ |preparations=Isolated mitochondria
  3 KB (411 words) - 16:37, 28 March 2018
• Luptak 2023 Antioxidants (Basel)
  ...duced changes in the activity of ETC complexes and the respiratory rate of mitochondria. In contrast, MAO inhibition could be linked to the antidepressant, procogn |preparations=Isolated mitochondria, Oxidase;biochemical oxidation
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• Giulivi 2008 Biochem J
  ...Horton AA, Luckhart S (2008) Metabolic pathways in ''Anopheles stephensi'' mitochondria. Biochem J 415:309-16. ...ed muscle mitochondria depend on the malate-oxaloacetate shuttle. Two, ASE mitochondria were able to oxidize proline at a rate comparable with that of alpha-glycer
  4 KB (555 words) - 18:53, 15 March 2021
• Scandroglio 2014 Free Radic Res
  ...s, and reactive species formation, we performed a metabolic analysis using isolated mitochondria from different rat tissues. Titrations with fluorocitrate showed IC<sub>50<
  2 KB (312 words) - 10:04, 8 November 2016
• Heim 2017 Am J Physiol Regul Integr Comp Physiol
  ...generally mild and inconsistent with functional variations. In conclusion, mitochondria respond to seasonal variations in physical activity, temperature, and nutri |keywords=Fatty acids, Hibernation, Metabolism, Mitochondria, Phospholipids, Respiration
  3 KB (364 words) - 17:54, 22 November 2017
• Gizatullina 2011 Mitochondrion
  ...nosuppressive or non-immunosuppressive derivatives [D-Ser]8-CsA and Cs9 on mitochondria from different brain regions. Mitochondrion 11(3):421-9. ...functional properties under control conditions. They also preserved brain mitochondria against Ca(2+) overload-mediated PT and functional impairments. Since Cs9 d
  3 KB (348 words) - 17:05, 7 November 2016
• Opalka 2002 Biochem Pharmacol
  ...trate, 50 μM of RO-28-2653 inhibited mitochondrial respiration in isolated mitochondria and skinned fibers by 23 and 11%, respectively while 2 mM of amytal elicite |keywords=2,4-Dinitrophenol, Amytal, Matrix metalloproteinases, Rat heart mitochondria, RO-28-2653
  3 KB (363 words) - 10:54, 23 January 2019
• Astin 2013 Sci Rep
  ...abadkai G (2013) No evidence for a local renin-angiotensin system in liver mitochondria. Sci Rep 3:2467. ...ed mitochondria. Moreover, AngII had no effect on the function of isolated mitochondria at physiologically relevant concentrations. We thus found no evidence of en
  2 KB (307 words) - 13:22, 23 January 2019
• Lou 2007 Biochem J
  ...additional effect. The wide dynamic range of these uncouplers in isolated mitochondria and intact cells reveals a novel allosteric activation of proton transport |preparations=Intact cells, Isolated mitochondria
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• Sottocasa 1967 J Cell Biol
  ...) An electron-transport system associated with the outer membrane of liver mitochondria. A biochemical and morphological study. J Cell Biol 32:415-38. ...l evidence supports the conclusion that the former subfraction consists of mitochondria devoid of outer membrane, and that the latter represents derivatives of the
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• Gainutdinov 2015 Arch Biochem Biophys
  ...) Knockout of cyclophilin D in Ppif(-/-) mice increases stability of brain mitochondria against Ca(2+) stress. Arch Biochem Biophys 579:40-6. ...bsence improves the complex I-related mitochondrial function and increases mitochondria stability against Ca(2+) stress.
  2 KB (348 words) - 20:37, 7 March 2020
• Miwa 2015 Free Radic Biol Med
  ...or the detection of H₂O₂ release from isolated liver mitochondria. Our results call for a serious re-examination of previous data. |keywords=Amplex Red, Mitochondria, Liver, Reactive Oxygen Species, Measurement, Carboxylesterase
  2 KB (329 words) - 21:40, 24 September 2021
• Hansson 2007 Thesis
  bioenergetic function of mitochondria. The objective of the present studies was to characterize the mPT phenomenon in isolated rodent brain mitochondria.
  3 KB (450 words) - 17:20, 7 November 2016
• Shirey 2016 Biochemistry
  ...ngin and micafungin both inhibit whole chain electron transfer in isolated mitochondria at low micromolar concentrations. The effects of the CLPs are fully reversi |preparations=Isolated mitochondria, SMP
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• Mottahedin 2017 J Cereb Blood Flow Metab
  ...emia (HI) in neonatal mice. High-resolution respirometry of brain isolated mitochondria revealed that P3C suppresses ADP-induced oxidative phosphorylation, the mai |preparations=Isolated mitochondria
  2 KB (217 words) - 17:19, 31 January 2020
• Staples 2010 Abstract IOC60
  ...d State 3 respiration rates of liver mitochondria, measured at 37 °C. When isolated from 13-lined ground squirrel (''Ictodomys tridecemlineatus'') in early ent |keywords=Hibernation, Mitochondria, Ictidomys, Ground squirrel
  2 KB (261 words) - 16:52, 27 March 2018
• Rajasekaran 2018 FASEB J
  ...mplex IV KCN-sensitive respiration were reduced in ''Hint2''^-/- mitochondria. Furthermore, HINT-2 associated with cardiolipin and glucose-regulated prot |preparations=Isolated mitochondria
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• Shabalina 2013 Abstract MiP2013
  ...B, Nedergaard J (2013) Comparative study of brown and white adipose tissue mitochondria in mice upon cold acclimation. Mitochondr Physiol Network 18.08. ...be questioned: Is UCP1 able to implement thermogenesis in brite/beige-fat mitochondria?
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• Hansson 2008 Free Radic Biol Med
  ...ér E (2008) Calcium-induced generation of reactive oxygen species in brain mitochondria is mediated by permeability transition. Free Radic Biol Med 45:284-94. ...T-mediated mitochondrial swelling. We conclude that while intact respiring mitochondria possess powerful antioxidant capability, mPT induces a dysregulated oxidati
  2 KB (332 words) - 17:34, 25 February 2020
• Gam 2018 Acta Diabetol
  ...trup K, Mathiesen ER, Damm P, Quistorff B (2018) Diabetes, myometrium, and mitochondria in pregnant women at term. Acta Diabetol 55:999-1010. ...ations did not support any structural alterations in the myometrium or its mitochondria.
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• OROBOROS 1995
  ...e high-resolution respirometric measurement of oxygen kinetics in isolated mitochondria and cultured cells.]]
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• Lashbrook 2016 Thesis
  |title=Lashbrook M (2016) Functional responses of cardiac and skeletal muscle mitochondria to short-term obesity: are all obesities the same? Master's Thesis 63pp. ...ract=Obesity is associated with metabolic alterations, specifically in the mitochondria, that may play a role in the development of insulin resistance and type 2 d
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• OROBOROS 1990
  ::::* Stoiber W: Fine structure of mitochondria in red and white muscle of cyprinid larvae. p. 40. ..., Gnaiger E: Ultrastructure and enzyme cytochemistry of aerobic and anoxic mitochondria of Lumbriculus variegatus. p. 46-48.
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• Petiz 2018 Chem Biol Interact
  ...N'-diphenyl-4-methoxy-benzamidine, on energy-linked functions of rat liver mitochondria. Chem Biol Interact 279:34-42. ...thoxyamidine has slight effects on the energy-linked functions of isolated mitochondria at concentrations that correspond to the LD₅₀ against ''Leishman
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• Iglesias-Gonzalez 2014 PhD Thesis
  |abstract=The study of mitochondria during the last years has revealed that this organelle plays an important r |preparations=Isolated mitochondria
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• Appaix 2002 Biochim Biophys Acta
  ...ieille C, Olivares J, Longuet M, Antonsson B, Saks VA (2002) Bax and heart mitochondria: uncoupling and inhibition of respiration without permeability transition. ...d kinetics of regulation of respiration were studied in isolated rat heart mitochondria and permeabilized cardiomyocytes. The results showed that while both Bax-FL
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• Evans 1983 Eur J Biochem
  ...T, Scragg AH, Ratledge C (1983) A comparative study of citrate efflux from mitochondria of oleaginous and non-oleaginous yeasts. Eur J Biochem 130:195-204. |abstract=Citrate efflux from mitochondria of 10 different yeasts was investigated.
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• Du 1998 Free Radic Biol Med
  ...G, Mouithys-Mickalad A, Sluse FE (1998) Generation of superoxide anion by mitochondria and impairment of their functions during anoxia and reoxygenation. Free Rad ...onal damage is related and secondary to superoxide anion production by the mitochondria ''in vitro''.
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• Loomis 1948 J Biol Chem
  |preparations=Isolated mitochondria
  645 bytes (76 words) - 17:44, 16 January 2021
• Brunmair 2004 Diabetes
  ...II was unaffected. In line with inhibition of Complex I, 24-h exposure of isolated rat soleus muscle to metformin or TZDs reduced cell respiration and increas |preparations=Intact organ, Isolated mitochondria
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• Shiva 2007 Methods Cell Bio
  ...n provide insight into intricate nature of the interaction between NO· and mitochondria and the variations in this system, which can occur under both physiological ...=Animals, Calibration, Cell Hypoxia, Cell Respiration, Electrodes, Humans, Mitochondria, Nitric Oxide, Oxygen, Oxygen Consumption and Reperfusion Injury
  2 KB (250 words) - 12:01, 6 October 2015
• Du 2016 J Exp Biol
  ...both patterns of hypoxia acclimation reduced the rate of ROS emission from mitochondria when compared at a common O₂ tension. Hypoxia acclimation also i |preparations=Isolated mitochondria
  2 KB (331 words) - 13:41, 7 March 2020
• Yu 2023 Biochim Biophys Acta Bioenerg
  ...o provide evidence that OAA decarboxylation clears OAA to pyruvate in IBAT mitochondria. |keywords=Brown adipose tissue, Mitochondria, Mitochondrial complex II, Muscle, Oxaloacetate, Succinate dehydrogenase
  3 KB (357 words) - 07:22, 28 February 2023
• Gizatullina 2005 Biochim Biophys Acta
  ...ffects of extramitochondrial ADP on permeability transition of mouse liver mitochondria. Biochim Biophys Acta 1706:98-104. ...ished the action about of 1 mM ADP, but was not able to induce swelling of mitochondria in the presence of 2 mM ADP.
  2 KB (301 words) - 15:07, 24 March 2015
• Gnaiger 2000 Life in the Cold
  ...AV, Schneeberger S, Seiler R, Brandacher G, Steurer W, Margreiter R (2000) Mitochondria in the cold. In: Life in the Cold (Heldmaier G, Klingenspor M, eds) Springe ...ates, ATP, histidine, and oncotic agents. Successful cold storage of heart mitochondria demonstrates a large scope for improvement of heart preservation solutions.
  3 KB (452 words) - 10:25, 5 May 2022
• Zholobenko 2017 PLOS ONE
  ...f quercetin observed in whole and permeabilised cells may originate in the mitochondria, while the mechanism of action of cardioprotection by dehydrosilybin may be
  3 KB (381 words) - 13:38, 29 May 2018
• Karbanova 2015 Abstract MiP2015
  ...flicting results have been published regarding the localization of CD36 in mitochondria and its direct role in mitochondrial FA transport and oxidation. ...m/mg prot/2h), suggesting that CD36 is not important for FA transport into mitochondria.
  3 KB (393 words) - 16:28, 4 September 2015
• Baum 1967 Nature
  |preparations=Isolated mitochondria
  639 bytes (77 words) - 16:51, 16 January 2021
• Safiulina 2004 J Neurosci Methods
  ...esses in the cell, and the easy manipulation of the milieu surrounding the mitochondria. Thus, the presented method offers the opportunity to study the neuronal mi
  2 KB (249 words) - 15:52, 8 June 2015
• Schiffer 2014 FASEB J
  level of efficiency that allows maximal power production. skeletal muscle mitochondria from the same individuals.
  3 KB (369 words) - 16:22, 27 March 2018
• Du 2017 Evolution
  ...control ratios of capacities for electron transport (measured in uncoupled mitochondria) relative to oxidative phosphorylation suggested that the limiting influenc |preparations=Isolated mitochondria
  2 KB (315 words) - 13:01, 7 March 2020
• Kolb 2018 J Am Soc Nephrol
  ...d rise in serum creatinine (P<0.05) observed in controls and increased the mitochondria membrane potential (P<0.05), maximal respiratory capacity (P<0.05), and int |preparations=Isolated mitochondria
  2 KB (314 words) - 15:20, 11 July 2018
• Luther 2023 Am J Physiol Renal Physiol
  ...sodium transport were repeatedly measured. Live cortical mitochondria were isolated at baseline and end of experiment and assessed ''in vitro'' with high resol |keywords=Acute kidney injury, Mitochondria, Sepsis
  3 KB (383 words) - 16:36, 2 May 2023
• Sharpe 2021 Front Oncol
  ...les completely arrest electron transport in isolated, respiring, rat liver mitochondria and patient derived glioblastoma (GBM), meningioma and diffuse intrinsic po |preparations=Isolated mitochondria
  2 KB (351 words) - 05:49, 6 February 2024
• Cordero-Reyes 2014 J Mol Cell Cardiol
  ...sueh WA, Torre-Amione G, Taegtmeyer H, Hamilton DJ (2014) Freshly isolated mitochondria from failing human hearts exhibit preserved respiratory function. J Mol Cel ...the failing LV myocardium are capable of tightly coupled respiration when isolated and supplied with ample substrates. Thus energy starvation in the failing h
  4 KB (532 words) - 17:25, 9 November 2017
• Day 1991 Plant Physiol
  ...ch JT, Moore AL (1991) Regulation of alternative pathway activity in plant mitochondria: deviations from Q-pool behavior during oxidation of NADH and quinols. Plan ...that some segregation of electron transport chain components may exist in mitochondria from nonthermogenic plant tissues.
  2 KB (321 words) - 12:41, 3 April 2021
• Oezen 2005 Hum Mol Genet
  ...y electron microscopy, revealed normal size, structure and localization of mitochondria in muscle of both groups. Similar to the results obtained in skeletal muscl |preparations=Permeabilized cells, Permeabilized tissue, Isolated mitochondria
  2 KB (312 words) - 13:32, 24 June 2019
• Munro 2022 Mitochondrion
  ...e elimination of ROS characterizes long-lived species, a novel property of mitochondria thus far demonstrated in two key biogerontological models from distant evol |keywords=Arctica islandica, Bivalves, Hydrogen peroxide, Longevity, Mitochondria, Reactive oxygen species (ROS)
  2 KB (273 words) - 19:17, 24 February 2023
• Hagl 2013 Pharmacol Res
  ...ggests improved mitochondrial function in brains of RBE fed animals. Cells isolated from brains of RBE fed animals showed significantly higher mitochondrial me |keywords=Bioenergetics, Mitochondria, Brain nutrition, Respiration, Cardiolipin, Mitochondrial dynamics, Nitrosa
  2 KB (351 words) - 10:49, 27 March 2018
• Aon 2015 J Gen Physiol
  ...ely, these results converge on the idea that GSH/Trx scavenging systems in mitochondria are both essential for keeping minimal levels of H₂O₂ |preparations=Isolated mitochondria
  3 KB (418 words) - 15:28, 7 November 2016
• Ost 2018 Curr Opin Clin Nutr Metab Care
  ...e biopsies (such as skeletal muscle, liver and adipose tissue), as well as isolated blood cells. ...approaches are available, certain well-established protocols for isolated mitochondria are limited for the analysis of mitochondrial function in tissue biopsies o
  2 KB (311 words) - 14:40, 5 July 2023

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