Difference between revisions of "MiPNet20.05 IOC104 Greenville"

Latest revision as of 15:14, 8 June 2020

MiPNet20.05 IOC104 Greenville

Greenville NC US, 2015 Aug 09-10. Oroboros O2k-Workshop on HRR and O2k-Fluorometry, IOC104.

»

Oroboros (2015-08-09) Mitochondr Physiol Network

Abstract:

Oroboros O2k-Workshop on high-resolution respirometry and O2k-Fluorometry »Versions

• O2k-Network Lab: US NC Greenville Neufer PD, US NC Greenville Brown DA, AT Innsbruck Oroboros

Labels:

HRR: Oxygraph-2k

ORO, IOC, O2k-Network, 2015, MitoFit

The IOC104 participants in front of the Heart Institute Building.

General Information

This O2k-Workshop takes place prior to the MiPschool Greenville 2015, from 9th to 10th August 2015. Separate registration for this workshop is required.

Contact

Darrel Neufer, Director, East Carolina Diabetes & Obesity Inst; Professor, Depts Physiology and Kinesiology, East Carolina Univ, US

David A Brown, East Carolina Diabetes & Obesity Inst; Associate Professor, Dept Physiology, East Carolina Univ, US

IOC support team

Carolina Doerrier, PostDoc, Oroboros Instruments, Innsbruck, AT

Erich Gnaiger, CEO, Oroboros Instruments, Innsbruck, AT

Chuck Hoppel, Director, Ctr Mitochondrial Diseases; Professor, Dept Pharmacology, Case Western Reserve Univ, Cleveland, Ohio, US

Laner Verena, Chief Operating Officer (COO), Oroboros Instruments, Innsbruck, AT

Assisted by the Greenville team: Ashley Busada, Peter Chien-Te Lin, Lauren Reese, Terence E Ryan, Cheryl Smith, Cody D Smith

Venue

All sessions were held in the conference facilities at the East Carolina Heart Institute on the health sciences campus of East Carolina University.

» East Carolina University, East Carolina Diabetes & Obesity Institute, East Carolina Heart Institute, Greenville, NC, USA

Feedback

Blue Ouroboros has been done and will be shipped today in a few hours to you. .. I was thinking about sexual dimorphism of Ouroboroi, and, according to my opinion, you're going to have a female one. I also decided to take a picture of nice coupled Ouroboroi. - Anna Stepanova, O2k-Network Lab: UK Belfast Galkin A (2015-08-20).

Thanks a lot to you and your team, for organizing such a fantastic workshop and MiPschool lectures. - Naeem Patil, O2k-Network Lab: US AR Little Rock MacMillan-Crow LA (2015-08-19).

It was a pleasure to attend the 9th Anuual MiPschool and O2K workshop. I learned a lot about quality assurance, applications of the oxygraph and LED2 module, and advancements in mitochondrial research. I am excited to bring my new knowledge and skill set back to the lab! - Teresa Ea, O2k-Network Lab: US PA Philadelphia Margulies S (2015-08-19).

Thank you for your intensive workshop in North Carolina. I have taken a lot back and preparing to get started with measuring H₂O₂ production and membrane potential in the days to come. - Nisha Bhattarai, O2k-Network Lab: US TX Galveston Porter C (2015-08-17).

It was a great workshop! - Shilpa Iyer, O2k-Network Lab: US VA Richmond Iyer S (2015-08-13).

Some impressions

The host P. Darrell Neufer is extending a welcome to all the participants
Verena Laner and Erich Gnaiger present the MitoFit banner: a welcome gift for P. Darrell Neufer and David A. Brown as a Power-O2k Network Lab
Peter showing a video of the fiber preparation.
Carolina Doerrier and Erich Gnaiger during the demo experiment on freeze-dried baker's yeast.
Discussion on a 'reference sample' for HRR with Chuck Hoppel and Erich Gnaiger.
Team Trivia Night with the winner team "the fibrebundels". From left to right: Eric Goetzman, Matthew Morris, Matthew Longo, Anders Gudiksen
Andy P. Li, Verena Laner, Erich Gnaiger, Pushpa Sharma
P. Darrell Neufer, Erich Gnaiger, Matthew Morris, Naeem Patil and Nicolas Houstis
Marla Perna, Nisha Bhattarai, Tray Wright, Ricardo Samms, Maria Chondronokila and Mark Wade
Discussion after the experiments with Carolina Doerrier and Erich Gnaiger
Conclusion by Chuck Hoppel

DatLab Files and O2k-Analysis

DatLab Files

» File:Cells-IOC104 2015-08-10 P8-03.DLD

» File:Cells H2O2-IOC104 2015-08-10 P2-02.DLD

O2k-Analysis

» File:O2k Perm cells cyt c IOC104 P8.xls

» File:O2k-AmplexRed cells H2O2 IOC104 P2.xls

@@ Line 3: / Line 3: @@
 {{Publication
 |title=[[Image:O2k-Network.png|right|60px|link=O2k-Network|O2k-Network]]
-'''Greenville NC US''', 2015 Aug 09-10. OROBOROS O2k-Workshop on HRR and O2k-Fluorometry, '''IOC104'''. [[Media:MiPNet20.05 IOC 2015-08 Greenville.pdf|»Bioblast pdf«]]
+'''Greenville NC US''', 2015 Aug 09-10. Oroboros O2k-Workshop on HRR and O2k-Fluorometry, '''IOC104'''.
-|info=[http://wiki.oroboros.at/index.php/File:MiPNet20.05_IOC_2015-08_Greenville.pdf Versions]
+|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/8/87/MiPNet20.05_IOC_2015-08_Greenville.pdf|Bioblast pdf]]
-|authors=OROBOROS
+|authors=Oroboros
 |year=2015-08-09
 |journal=Mitochondr Physiol Network
 |abstract=[[Image:O2k-Workshops.png|left|130px|link=O2k-Workshops|O2k-Workshops]]
-'''OROBOROS O2k-Workshop on high-resolution respirometry and O2k-Fluorometry'''
+'''Oroboros O2k-Workshop on high-resolution respirometry and O2k-Fluorometry''' »[http://wiki.oroboros.at/index.php/File:MiPNet20.05_IOC_2015-08_Greenville.pdf Versions]
-|mipnetlab=US NC Greenville Neufer PD, US NC Greenville Brown DA, AT Innsbruck OROBOROS
+|mipnetlab=US NC Greenville Neufer PD, US NC Greenville Brown DA, AT Innsbruck Oroboros
 }}
 {{Labeling
-|additional=ORO, IOC, MiPNet, 2015
+|instruments=Oxygraph-2k
+|additional=ORO, IOC, O2k-Network, 2015, MitoFit
 }}
 [[File:IOC104_Greenville2015_Group.jpg|800px|IOC104 participants]]
@@ Line 36: / Line 40: @@
-[[File:IOC104 OROBOROS-Team Carolina-Erich-Verena.jpg |right |300px |Carolina, Erich, Verena]]
 == IOC support team ==
 [[File:DoerrierC.JPG|80px|link=Doerrier C|Carolina Doerrier]]
-[[Doerrier C |Carolina Doerrier]], PostDoc, OROBOROS INSTRUMENTS, Innsbruck, AT
+[[Doerrier C |Carolina Doerrier]], PostDoc, Oroboros Instruments, Innsbruck, AT
 [[File:Gnaiger Erich.jpg|80px|link=Gnaiger E|Erich Gnaiger]]
-[[Gnaiger E |Erich Gnaiger]], CEO, OROBOROS INSTRUMENTS, Innsbruck, AT
+[[Gnaiger E |Erich Gnaiger]], CEO, Oroboros Instruments, Innsbruck, AT
 [[File:Hoppel 2012x.jpg|80px|Charles Hoppel]]
@@ Line 48: / Line 52: @@
 [[File:VG.jpg|80px|link=Laner V|Laner Verena]]
-[[Laner V |Laner Verena]], Chief Operating Officer (COO), OROBOROS INSTRUMENTS, Innsbruck, AT
+[[Laner V |Laner Verena]], Chief Operating Officer (COO), Oroboros Instruments, Innsbruck, AT
 : Assisted by the Greenville team: '''[[Busada A |Ashley Busada]], [[Lin CT |Peter Chien-Te Lin]], [[Reese L |Lauren Reese]], [[Ryan TE |Terence E Ryan]], [[Smith C |Cheryl Smith]], [[Smith CD |Cody D Smith]]'''
@@ Line 97: / Line 101: @@
 File:IOC104 12.JPG| Conclusion by Chuck Hoppel
 </gallery>
+== DatLab Files and O2k-Analysis==
+'''DatLab Files'''
+'''»''' [[File:Cells-IOC104 2015-08-10 P8-03.DLD|Cells-IOC104 2015-08-10 P8-03.DLD]]
+'''»''' [[File:Cells H2O2-IOC104 2015-08-10 P2-02.DLD|Cells H2O2-IOC104 2015-08-10 P2-02.DLD]]
+'''O2k-Analysis'''
+'''»''' [[File:O2k Perm cells cyt c IOC104 P8.xls|O2k Perm cells cyt c IOC104 P8.xls]]
+'''»''' [[File:O2k-AmplexRed_cells_H2O2_IOC104_P2.xls|O2k-AmplexRed_cells_H2O2_IOC104_P2.xls]]
 == Recommended reading ==
@@ Line 103: / Line 124: @@
 '''»''' [[MitoPedia: Respiratory states]]
-[[Image:P.jpg|link=OXPHOS capacity|OXPHOS]] [[Image:R.jpg|link=ROUTINE respiration|ROUTINE]] [[Image:E.jpg|link=ETS capacity|ETS]] [[Image:L.jpg|link=LEAK respiration|LEAK]]  [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]]
+[[Image:P.jpg|link=OXPHOS capacity|OXPHOS]] [[Image:R.jpg|link=ROUTINE respiration|ROUTINE]] [[Image:E.jpg|link=ET capacity|ETS]] [[Image:L.jpg|link=LEAK respiration|LEAK]]  [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]]