TPP+ inhibitory effect: Difference between revisions
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{{ | {{MitoPedia | ||
|description=A major task in establishing a procedure for measurement of [[mitochondrial membrane potential]] using probe molecules is the evaluation of inhibitory concentrations of the probe molecule on the activity of respiration. The '''TPP<sup>+</sup> inhibitory effect''' (this also applies to TPMP<sup>+</sup> and other indicator molecules) is frequently ignored. Accurate knowledge of a threshold concentration is required to evaluate the necessary limit of detection of TPP<sup>+</sup>, and for restriction of experimental TPP<sup>+</sup> concentrations below the inhibitory range. | |||
|info=[[MiPNet14.05]] | |||
}} | |||
{{Keywords: Force and membrane potential}} | |||
ย | {{MitoPedia methods | ||
{{MitoPedia | |mitopedia method=Respirometry | ||
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{{ | {{MitoPedia topics | ||
| | |mitopedia topic=Inhibitor | ||
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Latest revision as of 00:03, 18 February 2020
Description
A major task in establishing a procedure for measurement of mitochondrial membrane potential using probe molecules is the evaluation of inhibitory concentrations of the probe molecule on the activity of respiration. The TPP+ inhibitory effect (this also applies to TPMP+ and other indicator molecules) is frequently ignored. Accurate knowledge of a threshold concentration is required to evaluate the necessary limit of detection of TPP+, and for restriction of experimental TPP+ concentrations below the inhibitory range.
Reference: MiPNet14.05
- Bioblast links: Force and membrane potential - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Fundamental relationships
- ยป Force
- ยป Affinity
- ยป Flux
- ยป Advancement
- ยป Advancement per volume
- ยป Stoichiometric number
- Fundamental relationships
- mt-Membrane potential and protonmotive force
- ยป Uncoupler
- O2k-Potentiometry
- ยป O2k-Catalogue: O2k-TPP+ ISE-Module
- ยป O2k-Manual: MiPNet15.03 O2k-MultiSensor-ISE
- ยป TPP - O2k-Procedures: Tetraphenylphosphonium
- ยป Specifications: MiPNet15.08 TPP electrode
- ยป Poster
- ยป Unspecific binding of TPP+
- ยป TPP+ inhibitory effect
- O2k-Potentiometry
- O2k-Fluorometry
- ยป O2k-Catalogue: O2k-FluoRespirometer
- ยป O2k-Manual: MiPNet22.11 O2k-FluoRespirometer manual
- ยป Safranin - O2k-Procedures: MiPNet20.13 Safranin mt-membranepotential / Safranin
- ยป TMRM - O2k-Procedures: TMRM
- O2k-Fluorometry
- O2k-Publications
MitoPedia methods:
Respirometry
MitoPedia topics:
Inhibitor