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..., ADP and glutamate (GMO(3) ), + succinate (GMOS(3) ), + uncoupler (U) and inhibitor (rotenone) of complex I respiration. Citrate synthase (CS) activity was mea |keywords=AMPKα2 kinase-dead (KD) mice; substrate-uncoupler-inhibitor-titration (SUIT) protocol

...ma S, Yokota T, Tanaka S, Okita K, Tsutsui H (2016) Dipeptidyl peptidase-4 inhibitor improved exercise capacity and mitochondrial biogenesis in mice with heart ...igated the effects of the administration of a dipeptidyl peptidase (DPP)-4 inhibitor on the exercise capacity and skeletal muscle abnormalities in an HF mouse m

...d soluble F₁-ATPase. Activity assays and immunodetection of the inhibitor protein IF₁ were used and combined with molecular docking analys ''Key Results'': T1AM is a non-competitive inhibitor of F₀F₁-ATPsynthase whose binding is mutually exclusi

...r), ODQ (a GC inhibitor), PP2 (a src kinase inhibitor), and KT-5823 (a PKG inhibitor) abolished preconditioning by BK and blocked the inotropic response to ouab |topics=Cyt c, Inhibitor, Ion;substrate transport

...O(2)(-) generation in control cells, or those treated with the NO synthase inhibitor, when incubated at 21% O(2). However, after 30-min exposure of control cell |topics=Cyt c, Flux control, Inhibitor, Oxygen kinetics, Redox state

...Wang Z, Rhee KY, Ding K, Zhang T, Cook GM (2018) A pyrazolo[1,5-a]pyridine inhibitor of the respiratory cytochrome bcc complex for the treatment of drug-resista ...infection. Here, we characterize a putative respiratory complex III (QcrB) inhibitor, TB47: a pyrazolo[1,5-a]pyridine-3-carboxamide. TB47 is active (MIC between

...ediated pAkt while increased pAMPK regardless of insulin. Reciprocally, an inhibitor of Akt, triciribine (TCN), decreased cellular ATP contents. Overexpression

...) and diacylglycerol (DAG) content was significantly attenuated with DPP-4 inhibitor treatment. In addition, MK0626 significantly reduced mitochondrial incomple ...rds=Lipidomics, NAFLD, Obesity, Hepatic insulin resistance, MK-0626 (DPP-4 inhibitor)

...et''' contains the sequential titrations in a specific Substrate-uncoupler-inhibitor titration (SUIT) protocol. The laboratory titration sheets for different SU

...GA, Doshi A, Zaware N, Gangjee A, Ihnat MA (2016) AG311, a small molecule inhibitor of complex I and hypoxia-induced HIF-1α stabilization. Cancer Lett pii: S0 ...nd animals with AG311 and dichloroacetate, a pyruvate dehydrogenase kinase inhibitor that increases oxidative metabolism, resulted in synergistic cell kill and

...were divided into 2 groups, i.e., with or without treatment with the SGLT2 inhibitor empagliflozin (Empa, 300 mg/kg of food). Consistent with previous studies, ..., Ketone body, Mitochondria, Muscle strength, Myocardial infarction, SGLT2 inhibitor

|description='''P1,P5-Di(adenosine-5')pentaphosphate (Ap5A)''' is an inhibitor of [[adenylate kinase]] (ADK), the enzyme which rephosphorylates AMP to ADP |mitopedia topic=Inhibitor

...hydroxymalonate (an inhibitor of malic enzymes) and mercaptopicolinate (an inhibitor of phosphoenolpyruvate carboxykinase) as tools. Hydroxymalonate inhibited t

...xidase]] was inhibited by micromolar concentrations of [[antimycin A]], an inhibitor of mitochondrial respiration. The inhibition was observed with all three su |topics=Inhibitor

...nd cytochrome c release induced by the anti-apoptotic BCL-2 family protein inhibitor ABT-737.

...were within the same range as those observed for etomoxiryl-CoA, a potent inhibitor of CPT I. When a pancreatic INS(823/13), muscle L6E9, or kidney HEK293 cell

...uly U, Bir A, Chakrabarti S (2022) Cytotoxicity of mitochondrial Complex I inhibitor rotenone: a complex interplay of cell death pathways. https://doi.org/10.26 ...ages/6/64/Ganguly_2022_MitoFit.pdf Cytotoxicity of mitochondrial Complex I inhibitor rotenone: a complex interplay of cell death pathways]<br/>

...ACSF3) with enzymatic activity in the presence of [[malonate]] (Complex II inhibitor) and methylmalonate.

...oneutal and may be impaired by [[uncoupling]]. [[Aminooxyacetate]] is an [[inhibitor]] of the glutamate-aspartate carrier.

...nk JN, Maulucci G, Puglisi MA, Pani G, Gasbarrini A (2015) The multikinase inhibitor Sorafenib enhances glycolysis and synergizes with glycolysis blockade for c ...oxicity was dramatically increased by glucose withdrawal or the glycolytic inhibitor 2-DG. Under metabolic stress, activation of the AMP dependent Protein Kinas

|topics=Coupling efficiency;uncoupling, Inhibitor

...atory subunit). The hepatic monomer is larger than the muscle enzyme. Each inhibitor interacts via its thioester group at the palmitoyl-CoA binding site of the

...JH, Yook JI, Kang SG. (2022) Etomoxir, a carnitine palmitoyltransferase 1 inhibitor, combined with temozolomide reduces stemness and invasiveness in patient-de ...being realized. Etomoxir (ETO), a carnitine palmitoyltransferase 1 (CPT1) inhibitor exerts cytotoxic effects in GBM, which involve interrupting the FAO pathway

...the inner [[mitochondrial|mt]]-membrane of various tissues. Oa is a potent inhibitor of [[succinate dehydrogenase]].

|title=The novel Cyclophilin D inhibitor NVP019 reduces mitochondrial permeability transition in isolated rat and hu ...as to assess the potential of NVP019, a novel, non-immunosuppressive Cyp-D inhibitor with minimal off-target effects [3], to inhibit mitochondrial permeability

...hao Y, Li G, Liu T (2019) Empagliflozin, a sodium glucose co-transporter-2 inhibitor, alleviates atrial remodeling and improves mitochondrial function in high-f ...patients are less well-explored. We tested the hypothesis that the SGLT-2 inhibitor, empagliflozin, can prevent atrial remodeling in a diabetic rat model.

::::* Mitochondrial preparations: specific substrate-inhibitor combinations for selectively stimulating electron entry through Complex I ( ::::* Kinetic inhibitor control: Kinetic studies with variation of a specific inhibitor yield apparent kinetic constants, such as the ''K''_I'.

...ective effects of ICA on ROT-treated cells was markedly abolished by SIRT3 inhibitor 3-TYP. Our findings demonstrate that ICA exerts a neuroprotective role thro |topics=Inhibitor

...uly U, Bir A, Chakrabarti S (2022) Cytotoxicity of mitochondrial Complex I inhibitor rotenone: a complex interplay of cell death pathways. Bioenerg Commun 2022. ...iseases including Parkinson’s disease. Because the mitochondrial Complex I inhibitor rotenone is widely used to develop experimental models of Parkinson’s dis

|abstract=Rotenone is a naturally occurring mitochondrial complex I inhibitor with a known association with parkinsonian phenotypes in both human populat |topics=Inhibitor

|description=Mitochondrial '''Substrate-uncoupler-inhibitor titration''' ('''SUIT''') [[MitoPedia: SUIT |protocols]] are used with [[mi {{#ask:[[Additional label::Substrate-uncoupler-inhibitor titration]]

|mitopedia topic=Inhibitor

...ce of succinate the Km value for malate was 1.9 mM. ATP was found to be an inhibitor competitive with malate, with a Ki (ATP) of 0.2 mM. This is the first repor

|description='''Myxothiazol''' Myx is an inhibitor of [[Complex III]] (CIII). CIII also inhibits [[Complex I|CI]]. Myxothiazol ..., Xia D, Yu C-A, Xia J-Z, Kachurin AM, Zhang L, Yu L, Deisenhofer J (1998) Inhibitor binding changes domain mobility in the iron–sulfur protein of the mitocho

.../sub>= 360 nM Ca²⁺). This regulation was not affected by RR, an inhibitor of the mitochondrial Ca²⁺ uniporter. Active respiration is regul |topics=Calcium, Inhibitor

...se in FAD fluorescence in comparison to control. NaN3 (5 mM), a complex IV inhibitor, and CCCP (10 μM), a protonophore, caused decrease in NADH and FAD fluores

...i A, Mach T, Dembińska-Kieć A (2013) Metabolic effects of the HIV protease inhibitor – saquinavir in differentiating human preadipocytes. Pharmacol Rep 65:93 Methods: The aim of the study was to characterize effects of the protease inhibitor (PI) - saquinavir (SQV) on metabolic functions, and gene expression during

|title=Siebels I, Dröse S (2013) Q-site inhibitor induced ROS production of mitochondrial complex II is attenuated by TCA cyc ...ccupied flavin site. Importantly, the ROS production induced by the Q-site inhibitor atpenin A5 was largely unaffected by the redox state of the Q pool and the

...dicating that CII, not CIII, is the ROS-producing site. The complex I (CI) inhibitor rotenone partially reduces the ROS production driven by high succinate leve

...oxygen species (ROS) metabolism remains unclear. In this study a specific inhibitor for the rate-limiting enzyme involved in peroxisomal FAO, [[acyl-CoA oxidas |topics=Inhibitor

...neration, which was effectively prevented by edoxaban, vorapaxar and GB83 (inhibitor of protease-activated receptor 2). Of note, exposure to FXa did not initiat

...e transfer of electrons from heme ''b''_H to oxidized Q (Qi site inhibitor). High concentrations of antimycin A also inhibit acyl-CoA oxidase and D-am * [[Myxothiazol]] - this CIII inhibitor induces less ROS production but is more expensive than Ama.

...energy-producing pathways in the developing embryo. However, the V-ATPase inhibitor exhibits a concentration-dependent inhibition of oxygen consumption in aero

...l muscles. For that purpose skinned fiber technique and multiple substrate inhibitor titration were adapted to tumor samples. In our animal tumor model (R1H) fu ...Skinned fiber technique, High-resolution respirometry, Multiple substrate inhibitor titration, Rat rhabdomyosarcoma R1H

|topics=ADP, Inhibitor, Substrate, Uncoupler, Amino acid

...lectron donation by NADPH and ferredoxin to plastoquinone. Antimycin A (an inhibitor of cyclic electron flow) increased the NADH DH activity and preserved the

...ate from direct mPT inhibition. We conclude that a clinically relevant mPT inhibitor should have a mitochondrial target and increase mitochondrial calcium reten

...s with glycolytic inhibitors. Indeed, iodoacetate (IA), an effective GAPDH inhibitor, caused about 70% drop in MDA-MB-231 cell viability at 20 μM while 40 μM |topics=Inhibitor

...l loss in the brain of the stefin B-deficient mice, implying a role of the inhibitor at the cross-talk between microglia and cerebellar cells. Detailed analysis

...greater after azide than after myxothiazol or cyanide. Desferrioxamine, an inhibitor of iron-catalyzed hydroxyl radical formation, delayed cell killing after ea