Myxothiazol

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Myxothiazol

Description

Myxothiazol Myx is an inhibitor of Complex III (CIII). CIII also inhibits CI. Myxothiazol binds to the Qo site of CIII (close to cytochrome bL) and inhibits the transfer of electrons from reduced QH2 to the Rieske iron sulfur protein.

Abbreviation: Myx

Reference: MiPNet09.12

Application in HRR

Myx: Myxothiazol (from Myxobacterium myxococcus fulvus; C25H33N3O3S2), Sigma-Aldrich: T5580, store at 4 °C, CAS: 76706-55-3, M = 487.7 g·mol-1
Hazard statements: H300; fatal if swallowed
Available again from Sigma with this new product number. The old product number was M 5779.
Preparation of 1 mM storage and stock solution (dissolved in ethanol abs.) for 2-mL O2k-chamber:
  1. Weigh 1 mg of myxothiazol into a small glass vial.
  2. Add 2.05 mL ethanol.
  3. Divide into 0.2 mL (use glass vials).
  4. Store at -20 °C.
»O2k manual titrations MiPNet09.12
  • Titration volume (2-mL O2k-chamber): 1 µL using a 10 µL Hamilton syringe.
  • Final concentration: 0.5 µM.


Preparation of 0.25 mM stock solution (dissolved in ethanol abs.) for 0.5-mL O2k-chamber:
  1. Take 100 µL of the 1 mM stock solution.
  2. Add 300 µL EtOH.
»O2k manual titrations MiPNet09.12
  • Titration volume (0.5-mL O2k-chamber): 1 µL using a 10 µL Hamilton syringe.
  • Final concentration: 0.5 µM.

Compare

  • Antimycin A is less expensive than Myx but induces higher ROS production.


References

  • Kim H, Xia D, Yu C-A, Xia J-Z, Kachurin AM, Zhang L, Yu L, Deisenhofer J (1998) Inhibitor binding changes domain mobility in the iron–sulfur protein of the mitochondrial bc1 complex from bovine heart. Proc Natl Acad Sci U S A 95:8026-33. https://doi.org/10.1073/pnas.95.14.8026
  • Starkov AA, Fiskum G (2001) Myxothiazol induces H(2)O(2) production from mitochondrial respiratory chain. Biochem Biophys Res Commun. 281:645-50. https://doi.org/10.1006/bbrc.2001.4409
  • Von Jagow G, Engel WD(1981) Complete inhibition of electron transfer from ubiquinol to cytochrome b by the combined action of antimycin and myxothiazol. FEBS Lett 136:19-24. https://doi.org/10.1016/0014-5793(81)81206-9
  • Von Jagow G, Ljungdahl PO, Graf P, Ohnishi T, Trumpower BL (1984) An inhibitor of mitochondrial respiration which binds to cytochrome b and displaces quinone from the iron-sulfur protein of the cytochrome bc1 complex. J Biol Chem 259:6318–26. https://doi.org/10.1016/S0021-9258(20)82143-7




MitoPedia topics: Inhibitor