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From Bioblast
- ...protocols'''. [[Prespecified protocols |Prespecified]] and [[time-stamped protocols]] that are publicly deposited may help to save Millions of Euros that may o787 bytes (98 words) - 05:29, 19 July 2022
- ::::# [[MitoFit protocols]] ::::## [[Harmonization protocols]] (Begley, Ioannidis 2015)2 KB (222 words) - 06:12, 19 July 2022
- === Mitochondrial Bioenergetics : Methods and Protocols === .../doi/10.1007/978-1-61779-382-0_3 Mitochondrial Bioenergetics : Methods and Protocols]2 KB (242 words) - 17:49, 1 December 2020
- ...k-Protocols.jpg|right|150px|link=http://www.oroboros.at/?o2k-protocols|O2k-Protocols contents]] ...solved. Accuracy is improved by standard numerical corrections. Graphs and protocols are stored and printed ready for publication.2 KB (303 words) - 10:54, 11 January 2018
- The SUIT-004 protocols are designed to provide a quick assessment of the linear coupling control ( == Specific SUIT protocols ==2 KB (293 words) - 23:36, 12 April 2021
- ...Substrate-uncoupler-inhibitor titration''' ('''SUIT''') [[MitoPedia: SUIT |protocols]] are used with [[mitochondrial preparations]] to study respiratory control ==SUIT protocols==3 KB (339 words) - 08:57, 27 May 2020
- In events obtained from a selected [[DL-Protocols |protocol]], the entire sequence of consecutive events is defined with even »[[DL-Protocols#DL-Protocol_principles|Protocol events]]2 KB (396 words) - 11:45, 25 March 2022
- == Compare SUIT protocols ==3 KB (354 words) - 23:49, 12 April 2021
- ::: '''[[Categories of SUIT protocols |SUIT-category]]:''' FNS(Oct,GM) == Compare SUIT protocols ==2 KB (236 words) - 12:40, 17 September 2020
- ...Such conditions must be carefully evaluated, may require modifications of protocols, and must be corrected for acid-base reactions unrelated to glycolytic flux ...Such conditions must be carefully evaluated, may require modifications of protocols, and need data analysis beyond reporting changes of pH.4 KB (584 words) - 12:43, 1 March 2022
- ...overcome, providing a rationale for establishing a set of basic reference protocols. ...nce Camp 2016]]: Optimizing strategies on the malate concentration in SUIT protocols.3 KB (424 words) - 13:26, 3 April 2022
- ::: '''[[Categories of SUIT protocols |SUIT-category]]:''' N(PM) == Compare SUIT protocols ==2 KB (276 words) - 12:36, 25 November 2020
- == Specific SUIT protocols == == Compare SUIT protocols ==2 KB (325 words) - 23:49, 12 April 2021
- == Specific SUIT protocols == :::+ Harmonization with many SUIT protocols (up to step 7S).3 KB (407 words) - 13:24, 5 June 2019
- ...DatLab when recording the data with the O2k. Usng [[MitoPedia: SUIT |SUIT protocols]], a sequence of respiratory steady-states is measured, marks are set, and ...'': The default [[data recording interval]] is 2 s, applied with most SUIT protocols. The larger the data recording interval, the lower is the time resolution a4 KB (640 words) - 11:46, 25 March 2022
- ::: '''[[Categories of SUIT protocols |SUIT-category]]:''' FNS(Oct,PGM) == Compare SUIT protocols ==2 KB (292 words) - 11:41, 29 February 2024
- ::: '''[[Categories of SUIT protocols|SUIT-category]]:''' FNS(Oct,PGM) :::+ Harmonization with many SUIT protocols (up to step 7S).2 KB (345 words) - 10:29, 18 September 2020
- == Specific SUIT protocols == == Compare SUIT protocols ==3 KB (393 words) - 15:21, 8 June 2020
- ...Both methods are adaptable for compound screening; at the same time, these protocols provide easy-to-use and time- saving methods for ''in vitro'' studies of ce2 KB (250 words) - 16:18, 27 March 2018
- ...opose potential solutions for these problems, including '''improvements in protocols and documentation''', consideration of evidence from studies in progress, ' ::::* Make publicly available the full protocols, analysis plans or sequence of analytical choices, and raw data for all des10 KB (1,385 words) - 09:02, 28 June 2022
- ...control in high-resolution respirometry, aimed at standardization of SUIT protocols and fostering proficiency to generate experimental data of assured high qua ...using standardized mitochondrial reference samples, DatLab analysis, SUIT protocols and experimental design, normalization of flux and data reporting, O2k-Fluo5 KB (667 words) - 14:56, 11 July 2022
- ...provement over time. Ongoing quality assessment helps laboratories improve protocols and performance on a real-time basis to ensure optimal cryopreservation of2 KB (331 words) - 16:20, 12 February 2018
- ::: '''[[Categories of SUIT protocols |SUIT-category]]:''' FNS(Oct,GM) == Compare SUIT protocols ==3 KB (356 words) - 11:45, 29 February 2024
- == Specific SUIT protocols == == Compare SUIT protocols ==3 KB (400 words) - 12:36, 25 November 2020
- ...lign: top;"> [[File:Set_upper_O2_limit_in_menu_bar.png|thumb|none|220px|'''Protocols menu''']] </li> <!--[[File:Set_lower_O2_limit_in_menu_bar.png|thumb|200px|'''Protocols menu''']]3 KB (510 words) - 11:07, 25 March 2022
- == Specific SUIT protocols == == Compare SUIT protocols ==3 KB (412 words) - 12:33, 25 November 2020
- ...s, the present authors decided to investigate whether recurrent concussion protocols alter depressive-like phenotype behavior, and whether mitochondria play an2 KB (288 words) - 18:26, 6 November 2020
- === Protocols === ::::» [[Instrumental: Browse DL-Protocols and templates]]5 KB (701 words) - 13:36, 8 May 2024
- :::: '''[[MitoPedia: SUIT]] - first steps towards a data bank of SUIT protocols''' :::: '''Updated terminology with SUIT protocols - open for discussion:''' [[Lemieux 2017 bioRxiv 103457 |Lemieux H, Blier P2 KB (316 words) - 16:34, 27 February 2018
- Substrate-uncoupler-inhibitor-titration (SUIT) protocols are a combination of different substrates, inhibitors and uncouplers to ass In this outline two different SUIT protocols are described analyzing the difference in LEAK respiration measured with di4 KB (547 words) - 14:38, 5 July 2023
- The SUIT-012 protocols specifically focus on assessing the coupling control ([[LEAK respiration|'' == Specific SUIT protocols ==4 KB (505 words) - 23:57, 12 April 2021
- Numerous and different protocols have been developed or adapted to this subject. One However, the usual protocols using cuvette photometers can be both time consuming and4 KB (488 words) - 12:56, 27 June 2019
- == Specific SUIT protocols == == Compare SUIT protocols ==4 KB (543 words) - 15:19, 8 June 2020
- ::::* Introduction to SUIT protocols for simultaneous measurement of O2 flux and H₂O₂ flux :::::* Substrate-uncoupler-inhibitor titration (SUIT) protocols </div> </div>3 KB (332 words) - 12:27, 28 July 2020
- ::: '''[[Categories of SUIT protocols |SUIT-category]]:''' N(PGM) == Compare SUIT protocols ==3 KB (412 words) - 00:14, 13 April 2021
- ::: '''[[Categories of SUIT protocols |SUIT-category]]:''' FNS(PalOct,PGM) == Compare SUIT protocols ==3 KB (424 words) - 14:38, 17 September 2020
- ...S analysis with respiratory substrate-uncoupler-inhibitor titration (SUIT) protocols when working with isolated mitochondria, permeabilized cells or tissue homo2 KB (287 words) - 15:23, 5 September 2022
- ...etermine N-pathway [[Oxidative phosphorylation| ''P'']] capacity. SUIT-014 protocols provide an assessment of the coupling control [[LEAK respiration|''L'']]-[[ == Specific SUIT protocols ==4 KB (634 words) - 15:21, 8 June 2020
- == O2k-Core manual and selected protocols ==3 KB (389 words) - 11:45, 14 April 2021
- ...mples is different between protocols and between the groups adopting these protocols. ...for an open discussion about these approaches in order to standardize the protocols for better reliability and reproducibility.3 KB (451 words) - 14:39, 3 October 2019
- ...A copy number in pluripotent and differentiating cells using real-time PCR protocols; assess expression of the mtDNA specific replication factors through real-t2 KB (302 words) - 15:07, 13 November 2017
- ...be examined using reference substrate-uncoupler-inhibitor titration (SUIT) protocols, and evaluated conditions for cell storage for later and reproducible respi2 KB (322 words) - 18:43, 10 January 2022
- ...lign: top;"> [[File:Set_lower_O2_limit_in_menu_bar.png|thumb|none|220px|'''Protocols menu''']] </li> <!--[[File:Set_lower_O2_limit_in_menu_bar.png|thumb|200px|'''Protocols menu''']]5 KB (737 words) - 13:05, 24 March 2022
- ::: '''[[Categories of SUIT protocols |SUIT-category]]:''' NS(PGM) == Compare SUIT protocols ==3 KB (487 words) - 11:39, 29 February 2024
- ::: '''[[Categories of SUIT protocols |SUIT-category]]:''' FNS(Oct,PGM) == Compare SUIT protocols ==3 KB (496 words) - 12:08, 17 September 2020
- == Specific DLP protocols == ...nd [[SUIT 003 AmR ce D059]] with carrier titration as a control. These two protocols have to be done in parallel.6 KB (864 words) - 23:56, 12 April 2021
- ::::# Titration vol. [µL]: In SUIT protocols, the value is entered as the volume of a titration, for calculating the dil4 KB (559 words) - 09:44, 14 April 2024
- == Compare SUIT protocols ==3 KB (397 words) - 00:12, 13 April 2021
- ..., to address specific research interests, samples available, selected SUIT protocols, for further discussion with the Oroboros tutors. :::::» [[MitoPedia: SUIT|Library of SUIT protocols]]4 KB (567 words) - 13:58, 3 March 2021
- ...ific research questions. Many optimized SUIT protocols are available as DL-Protocols and will be shown during the training. The [[Gnaiger 2020 BEC MitoPathways| ...xtremely helpful and provide much guide and information regarding the O2k, protocols and bioenergetics.4 KB (577 words) - 15:03, 14 September 2021
- ..., to address specific research interests, samples available, selected SUIT protocols, for further discussion with the Oroboros tutors. :::::» [[MitoPedia: SUIT|Library of SUIT protocols]]4 KB (571 words) - 14:59, 6 September 2021
- ::::* Benefit from the unique features of [[DatLab |DatLab 7]] with [[SUIT protocols]]3 KB (397 words) - 15:34, 23 November 2021
- ...-Analysis templates are made available for specific [[MitoPedia: SUIT|SUIT protocols]], to express respiration as [[oxygen flux]] or [[oxygen flow]] normalized ...emplate for your specific SUIT protocol. In DatLab, in the pull-down menu 'Protocols: SUIT: Browse DL-Protocos and templates', select your specific SUIT protoco4 KB (574 words) - 11:36, 25 March 2022
- ...sions and new, user-friendly features on [[Run DL-Protocol/Set O2 limit|DL-protocols]], [[Export DL-Protocol User (*.DLPU)|DL-Protocol User]], quality control, ...ds= DatLab, [[MiPNet22.16 DatLab 7 Innovations: DL-Protocols|Innovation DL-Protocols]]10 KB (1,339 words) - 09:33, 6 May 2021
- ...measured, in WS and NWS, using two substrate-uncoupler-inhibitor titration protocols, at baseline (day 0), day 3 and 10 post-wounding, in STZ-DM and non-diabeti2 KB (342 words) - 15:50, 21 November 2023
- ...month interventions. Mitochondrial respiration was measured using two SUIT protocols in permeabilized muscle fiber bundles. Data were analyzed using a per-proto One hundred participants completed the ''a priori'' defined intervention protocols: (EX, ''N''=44; CON, ''N''=56). Weight loss was similar for both groups (Po3 KB (465 words) - 16:04, 7 November 2016
- ...is involved in redox homeostasis, particularly in the brain. In all, these protocols allow a detailed characterization of mitochondrial function in human neural3 KB (357 words) - 18:38, 16 September 2020
- ...of titration volumes and automatic control of complex titration-injection protocols. The TIP2k was specially developed for the O2k. The main modes of operation ::* Examples of proper TIP2k use and cleaning protocols8 KB (1,344 words) - 11:09, 18 October 2023
- ::::* Particularly the DL-Protocols may be of great interest to the expert O2k-users: ::::::» [[DL-Protocols]]5 KB (683 words) - 14:44, 24 August 2023
- ...n and controversial interpretation (Chacko et al 2014). Respirometric SUIT protocols for OXPHOS analysis (Sjoevall et al 2010, 2013, Pecina et al 2014) need to5 KB (672 words) - 13:27, 3 April 2022
- ...(95% CI: 0.928, 0.982), P < 0.001. Both the original and modified THRIFTY protocols generated type I and type II fibre pools of absolute purity. Using THRIFTY,3 KB (465 words) - 15:53, 19 December 2023
- ...|Bioblast pdf]][[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols contents]]5 KB (638 words) - 00:30, 5 March 2022
- ...mmercial- and working-'''solutions''' for improved consistency in relevant protocols. * In various protocols described on the Bioblast wiki, clarification and updates are required to p5 KB (791 words) - 02:57, 23 January 2023
- :::#:Depending on whether or not you use [[Run_DL-Protocol/Set_O2_limit | DL-protocols]], chose:6 KB (916 words) - 20:54, 11 June 2023
- ...possible to choose multiple research questions at a time, to find the best protocols that address this combination of problems. ...various combinations to provide links to the best [[MitoPedia: SUIT |SUIT protocols]]:4 KB (431 words) - 06:51, 3 November 2022
- == Compare SUIT protocols == :::* [[SUIT-018]] are short protocols to study the oxygen dependence of O<sub>2</sub> flux and H<sub>2</sub>O<sub5 KB (681 words) - 00:11, 13 April 2021
- The SUIT-011 protocols are designed to study physiologically relevant maximum mitochondrial respir == Specific SUIT protocols ==6 KB (951 words) - 15:35, 8 June 2020
- ...for application of complex substrate-uncoupler-inhibitor titration (SUIT) protocols which are the basis for investigating mitochondrial pathways and respirator3 KB (379 words) - 13:09, 14 February 2023
- ...sts and advanced diagnostic substrate-uncoupler-inhibitor titration (SUIT) protocols within a single assay for exploring mitochondrial function.6 KB (872 words) - 12:49, 31 August 2023
- ...ysis, we used two substrate-uncoupler-inhibitor titration (SUIT) reference protocols, RP1 and RP2, interrogating 20 respiratory states. These include fatty acid ...cts, offering a more sensitive diagnostic approach compared to respiratory protocols with non-permeabilized living cells.3 KB (478 words) - 13:44, 18 April 2023
- ...a Sumbalova]], [[Krumschnabel G |Gerhard Krumschnabel]]: Towards reference protocols. * Standard protocols for blood cell separation, cell type characterization (purity, composition)5 KB (615 words) - 11:51, 30 November 2017
- ...ototype test scenario and expanding the range of application-specific SUIT protocols in the MitoFit-data base. O2k-Core applications are extended by application3 KB (444 words) - 13:26, 3 April 2022
- ...nd [[SUIT-003 AmR ce D059]] with carrier titration as a control. These two protocols should be done in parallel. == Compare SUIT protocols ==5 KB (832 words) - 00:10, 13 April 2021
- ...tate]]s. A [[MitoPedia:_SUIT|library]] of evaluated and tested standard DL-Protocols is provided by the Oroboros team. The Titration-Injection-microPump [[TIP2k <div id="DL-Protocols-anchor1"></div>33 KB (4,934 words) - 12:45, 12 December 2022
- ...peroxide production. Many optimized [[SUIT protocols]] are available as DL-Protocols. The [[SUITbrowser]] helps you find the best SUIT protocol for your specifi5 KB (646 words) - 14:51, 28 September 2022
- ...ed out. Even so, we quite obviously lack unequivocal terminology, standard protocols, and perhaps most surprisingly, a common conceptual basis when performing s2 KB (282 words) - 15:55, 9 November 2016
- *'Specific protocols and procedures for cells and isolated mito.' *'The protocols and the specifications.'8 KB (1,269 words) - 10:08, 23 January 2019
- ...[Oroboros O2k-technology]]. Substrate-uncoupler-inhibitor titration (SUIT) protocols allow the interrogation of numerous mitochondrial pathway and coupling stat4 KB (482 words) - 09:09, 2 April 2024
- ...iminish these conditions, but some high intensity interval training (HIIT) protocols have shown similar improvements in insulin sensitivity, in spite of the red3 KB (448 words) - 15:41, 5 March 2019
- ...endence only on limited amount of canonical substrates. Furthermore, these protocols can be very helpful for identifying substrates of other mitochondrial metab :::: During my STSM , I will be focusing on development of protocols for permeabilized cells of immortalized murine brown adipocyte line (BMC).12 KB (1,689 words) - 16:20, 16 March 2020
- ...mption in real-time with high sensitivity in various tissues and substrate protocols. While most of the studies rely on saturating concentrations of substrates2 KB (328 words) - 12:05, 15 February 2024
- ...membrane potential). Many optimized [[SUIT protocols]] are available as DL-Protocols. The [[SUITbrowser]] helps you find the best SUIT protocol for your specifi6 KB (861 words) - 12:36, 29 April 2024
- ...Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and is ...pdf|O2k-brief]][[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]]6 KB (850 words) - 12:27, 5 March 2024
- S(Rot) pathway in the LEAK state can be evaluated in the following SUIT protocols: S(Rot) pathway in the OXPHOS state can be evaluated in the following SUIT protocols:8 KB (1,053 words) - 08:04, 30 March 2023
- ...ds Mol Biol|Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscl4 KB (577 words) - 12:24, 25 November 2020
- == Compare SUIT protocols == ::::* [[SUIT-008]] 1PM;2D;3G;4S;5U;6Rot-: SUIT-008 protocols are designed to assess the additivity between the [[NADH_Electron_transfer-5 KB (784 words) - 11:18, 17 September 2020
- ...ls, based on the latest experience with [[Pesta_2012_Methods_Mol_Biol|SUIT protocols]] developed for functional mitochondrial diagnosis. Catherine trained Austi ...e achieved by continued instrumental development, advancing and testing of protocols for functional mitochondrial diagnosis, and international cooperation. A sp6 KB (939 words) - 16:30, 2 January 2024
- ...h weight per experimental run, supporting application of complex titration protocols.</div>7 KB (949 words) - 11:36, 8 November 2023
- ...iable data in commonly used substrate-uncoupler-inhibitor-titration (SUIT) protocols and cyanide titration is needed to obtain precise data for COX excess capac ...hway and coupling control on mitochondrial ''p''<sub>50</sub>. During SUIT protocols, kinetic data for oxygen was obtained with aerobic-anaerobic transitions in5 KB (718 words) - 18:53, 10 January 2022
- === SUIT protocols for high-resolution respirometry === ::::* Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscl7 KB (992 words) - 02:24, 9 October 2019
- ...ific research questions. Many optimized SUIT protocols are available as DL-Protocols and will be shown at the O2k-Coaching Days.7 KB (955 words) - 10:50, 22 November 2021
- ...n on 96-well SPA plates monitored noninvasively in real time. (2) Numerous protocols designed for the measurement of cellular metabolism use <sup>14</sup>C-labe4 KB (536 words) - 14:31, 12 January 2018
- ...nson's, and Huntington's diseases. Here we provide detailed guidelines and protocols as standardised across the five collaborating laboratories of the CeBioND c3 KB (485 words) - 18:07, 13 September 2023
- :::: '''Standard operating procedures and user requirement document: Protocols, terminology, documentation''' ::::» [[WG1 MitoEAGLE protocols, terminology, documentation |WG1 Action]]11 KB (1,507 words) - 12:05, 19 January 2018
- ...Mitchell T, Darley-Usmar VM (2014) Bioenergetics and the oxidative burst: protocols for the isolation and evaluation of human leukocytes and platelets. J Vis E2 KB (333 words) - 14:59, 13 November 2017
- ...oupler-inhibitor titration|respiratory flux at various steady-states, SUIT protocols]]. ...omplemented by lectures on mitochondrial physiology (general topics; [[O2k-Protocols]]). Short presentations of 'O2k perspectives' provide participants with the8 KB (1,139 words) - 09:41, 1 June 2023
- ...and substrate control states [1] were established in 6 different [[SUIT]] protocols. In calculation of ΔΨ, mitochondrial protein was normalized to citrate sy |keywords=ΔΨ, Mitochondrial preparations, [[SUIT]] protocols4 KB (522 words) - 18:26, 10 January 2022
- | Discussion about biological samples, experimental design, SUIT protocols <br>6 KB (752 words) - 12:53, 7 November 2023
- ...gh-resolution respirometry of intact human peripheral blood cells. Several protocols were used – with and without rotenone inhibition of CI in the screening c ...e increase in oxygen consumption was ruled out using specific respirometry protocols and flow cytometry (assessing mitochondrial membrane potential using the pr4 KB (550 words) - 17:35, 25 February 2020
- ...atio]] (RCR) [6,7]. Applicaton of [[CI+II]] substrate combinations in SUIT protocols extends conventional bioenergetic studies to the level of mitochondrial phy ...ds Mol Biol|Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscl6 KB (822 words) - 11:03, 9 April 2022
