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High-resolution respirometry

From Bioblast

high-resolution terminology - matching measurements at high-resolution

High-resolution respirometry



High-resolution respirometry, HRR, is the state-of-the-art approach in mitochondria and cell research to measure respiration in various types of mitochondrial preparations and living cells combined with MultiSensor modules. Mitochondrial function and dysfunction have gained increasing interest over the past years, reflecting growing awareness of the fact that mitochondria play a pivotal role in human health and disease. Combining instrumental accuracy and reliability with the versatility of applicable protocols - allowing practically unlimited addition and combination of substrates, inhibitors, and uncouplers - mitochondrial respiratory pathways may be analyzed in detail to evaluate even minor alterations in respiratory pathway control and/or capacity. The most advanced way to analyze mitochondrial function is by means of high-resolution respirometry with the Oroboros O2k. The O2k is a sole source apparatus, with no other available instrument meeting its specifications for high-resolution respirometry. Substrate-uncoupler-inhibitor titration (SUIT) protocols allow the diagnosis of numerous mitochondrial pathways and coupling defects in a single respirometric assay. Technologically, HRR is based on the Oroboros O2k, combining optimized chamber design, application of oxygen-tight materials, electrochemical sensors, Peltier-temperature control, and specially developed software features (DatLab) to obtain the unique sensitive and quantitative resolution of oxygen concentration and oxygen flux, with both, a closed-chamber or open-chamber mode of operation (TIP2k). Standardized calibration of the polarographic oxygen sensor (static sensor calibration), calibration of the sensor response time (dynamic sensor calibration), and evaluation of instrumental background oxygen flux (systemic flux compensation) provide the experimental basis for high accuracy of quantitative results and quality control in HRR. HRR can be extended for MultiSensor analysis by using the O2k-FluoRespirometer. Smart Fluo-Sensors are integrated into the O2k to measure simultaneously fluorometric signals using specific fluorophores. Potentiometric modules are available with ion-selective electrodes (pH, TPP+). The NextGen-O2k is the all-in-one device including the Q-redox sensor and a PhotoBiology (PB) module.

Abbreviation: HRR

Reference: Oroboros O2k

O2k-Core to FluoRespirometer

  • Power O2k-Respirometer: Several O2k units are operated simultaneously (P1, P2, ..) to combine high-resolution respirometry with high-output.

MitoPedia: O2k and high-resolution respirometry

» MitoPedia: O2k hardware
» MitoPedia: DatLab
» MitoPedia: SUIT


» MitoPedia: Respiratory states OXPHOS ROUTINE ET capacity LEAK - ROX
» Respirometry
» MitoPedia: Respirometry
» Wikipedia: Respirometry
» O2k Applications

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O2k-Publications in the MiPMap

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MitoPedia methods: Respirometry, Fluorometry, Spectrophotometry 

MitoPedia O2k and high-resolution respirometry: O2k hardware, O2k-Respirometry, O2k-FluoRespirometry