SUIT-003 O2 ce D012

high-resolution terminology - matching measurements at high-resolution

SUIT-003 O2 ce D012

Description

Abbreviation: ceN(P)

Reference: A - SUIT-003 coupling control protocol (CCP) with pyruvate

SUIT number: D012_ce1;ce2P;ce3Omy;ce4U;ce5Rot;ce6Ama

O2k-Application: O2

SUIT-category: ceN(P)

SUIT protocol pattern: complex cell coupling control protocol ce1;ce2P;ce3Omy;ce4U;ce*

Steps and respiratory states

Step	State	Comment - Events (E) and Marks (M)
ce1	ROUTINE	ce1 ROUTINE respiration in the physiological coupling state R. Externally added permeable substrates could contribute to this respiratory state.
ce1P	ROUTINE	ce1;ce1P. Pyruvate, ROUTINE, R ROUTINE respiration in the physiological coupling state R. Externally added permeable substrates could contribute to this respiratory state.
ce2Omy	L	ce1;ce1P;ce2Omy Non-phosphorylating resting state (LEAK state); LEAK respiration L(n) in the absence of ADP, ATP, AMP (no adenylates).
ce3U	E	ce1;ce1P;ce2Omy;ce3U Uncoupler titration (avoiding inhibition by high uncoupler concentrations) to obtain electron transfer (ET) capacity E (noncoupled ET-state). Test for limitation of OXPHOS capacity P by the phosphorylation system (ANT, ATP synthase, phosphate transporter) relative to ET capacity E in mt-preparations: E-P control efficiency and E-L coupling efficiency. In living cells: E-R control efficiency and E-L coupling efficiency. Noncoupled electron transfer state, ET state, with ET capacity E.
ce4Rot	ROX	ce1;ce1P;ce2Omy;ce3U;ce4Rot Rox is the residual oxygen consumption in the ROX state, due to oxidative side reactions, estimated either after inhibition of CIII (e.g. antimycin A, myxothiazol), CIV (e.g. Cyanide) or in the absence of endogenous fuel-substrates. Rox is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration.
ce5Ama	ROX	ce1;ce1P;ce2Omy;ce3U;ce4Rot;ce5Ama Rox is the residual oxygen consumption in the ROX state, due to oxidative side reactions, estimated after addition of antimycin A (inhibitor of CIII). Rox is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration (mt).

MitoPedia concepts: SUIT protocol, SUIT A, Find

MitoPedia methods: Respirometry

References