- high-resolution terminology - matching measurements at high-resolution
Oxygen signal
Description
The oxygen signal of the O2k is transmitted from the electrochemical polarographic oxygen sensor (OroboPOS) for each of the two chambers to DatLab. The primary signal is a current mAmp which is converted into a voltage [V], and calibrated in SI units for amount of substrance concentration [µmol.dm-3 or µM].
Reference: MiPNet19.18D O2k-Calibration, MiPNet06.03 POS-Calibration-SOP
MitoPedia methods: Respirometry, "DatLab" is not in the list (Respirometry, Fluorometry, Spectrophotometry) of allowed values for the "MitoPedia method" property. DatLab
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
Stability of the oxygen signal
The stability of the oxygen signal of the O2k is evaluated by the (negative) uncorrected slope over time. After smoothing, the slope of a stable sensor should be less than +- 1 pmol.s-1.ml-1 in a 2-ml chamber.
- Instability of the oxygen signal
- There is drift of the oxygen signal over short or long periods of time.
- The signal at air saturation is abnormally high for a given gain setting.
- The zero current is high (>2.5%) and may show drift.
- Confirm and locate the problem by performing a Sensor test and following Locating a problem.
- Follow the standard instructions for oxygen sensor service.
