Difference between revisions of "Oroboros O2k-technology"
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{{Product | {{Product | ||
|description='''Oroboros O2k''' - the modular system for [[high-resolution respirometry]] and fluorometry for mitochondria and cell research, using small amounts of biological samples. The [[O2k-Fluorometer]] is the basis for combined measurement of respiration and fluorometric signals, supported by the [[Titration-Injection microPump]] and [[DatLab |DatLab 7]] ('''''new'''''). The modular O2k-concept yields a high flexibility of fluorespirometry with add-on O2k-MultiSensor Modules. | |description='''Oroboros O2k''' - the modular system for [[high-resolution respirometry]] and fluorometry for mitochondria and cell research, using small amounts of biological samples. The [[O2k-Fluorometer]] is the basis for combined measurement of respiration and fluorometric signals, supported by the [[Titration-Injection microPump]] and [[DatLab |DatLab 7]] ('''''new'''''). The modular O2k-concept yields a high flexibility of fluorespirometry with add-on O2k-MultiSensor Modules for research in mitochondrial physiology and pathology. | ||
|product id=1'''####''' | |product id=1'''####''' | ||
|product type=O2k, Catalogue | |product type=O2k, Catalogue | ||
|info=[[ | |info=[[Oroboros O2k-Catalogue]], [[Gnaiger_2008_POS]]. [[MiPNet06.01 O2k-Overview]] | ||
|product image= | |product image= | ||
}} | }} | ||
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::: '''» Keywords''' | ::: '''» Keywords''' | ||
<div class="mw-collapsible-content"> | <div class="mw-collapsible-content"> | ||
::::» [[MiPNet14.10| Top 10 Reasons]] for OROBOROS INSTRUMENTS | |||
::::» [[Purchase#Low_running_costs| Quality and economy]]: high-output, low running costs | |||
::::» [[O2k-Core|2-chamber high-resolution respirometer]] | ::::» [[O2k-Core|2-chamber high-resolution respirometer]] | ||
::::» [[High-resolution respirometry]] | ::::» [[High-resolution respirometry]] | ||
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== Oroboros O2k: development and distribution == | == Oroboros O2k: development and distribution == | ||
:::: The O2k is developed by [[OROBOROS INSTRUMENTS]] in cooperation with [http://www.wgt.at WGT] and produced in Austria by WGT. | ::::* The O2k is developed by [[OROBOROS INSTRUMENTS]] in cooperation with [http://www.wgt.at WGT] and produced in Austria by WGT. | ||
:::: O2k- | ::::* O2k-Fluorometry: HRR combined with measurement of ROS production, mitochondrial membrane potential, ATP production, Ca<sup>2+</sup>, pH. | ||
:::: Auxiliary Tools provide extended support in the context of high-resolution respirometry and O2k-Fluorometry: [[PBI-Shredder O2k-Set]] for tissue preparation; [[Microbalance-Set|Microbalance]], [[O2k-Dissection Set]]. | ::::* [[TIP2k-Module|Titration-Injection microPump TIP2k]]: The O2k-TIP2k combination allows operation with programmable titration regimes, feedback control, and at steady-states, yielding a new flexibility in experimental design by combining the technical advantages of closed and open systems approaches. Automatic titrations for kinetics; feedback-controlled instrumental background tests; respiration in a closed or open system, in aerobic-anaerobic transitions or at steady-state oxygen concentrations, covering the entire physiological oxygen range and the extremes of hypoxia. | ||
::::* O2k-Modules can be combined in any configuration with the O2k-Fluorometer, and can be added any time to extend the O2k according to application-specific objectives: [[O2k-TPP+ ISE-Module|potentiometry]] (pH, TPP+ or TPMP+ for mitochondrial membrane potential, Ca<sup>2+</sup>) and [[O2k-NO Amp-Module|amperometry]] (NO, H<sub>2</sub>S). | |||
::::* Auxiliary O2k-Tools provide extended support in the context of high-resolution respirometry and O2k-Fluorometry: [[MiPNet19.14 SOP Hamilton microsyringes|Microsyringes]], [[PBI-Shredder O2k-Set]] for tissue preparation; [[Microbalance-Set|Microbalance]], [[O2k-Dissection Set]]. | |||
[[File:Tube and Syringe Racks with O2k.JPG|right|240px|Tube and Syringe Racks with O2k]] | |||
== An Integrated Systems Approach: High-resolution fluorespirometry == | |||
[[File:O2k-Core basic.jpg|right|400px]] | |||
:::: The Oroboros O2k is the unique state-of-the-art instrument for HRFR - where high-resolution counts: | |||
::::* with small amounts of cells or tissue, | |||
::::* at low respiratory activities, | |||
::::* multiple substrate-uncoupler-inhibitor titrations (SUIT protocols) with resolution of changes in oxygen flux over prolonged incubation times, | |||
::::* flexibility of O2k-MultiSensor applications, | |||
::::* fast transitions at low oxygen levels, | |||
::::* stability and quality control. | |||
:::: High-resolution respirometry is the result of our long-term expertise in instrumental design, software development, mitochondrial physiology, clinical and pharmacological applications, and [[O2k-Network| world-wide scientific cooperation]]. The innovative O2k-developments provide a system of highest quality and distinguish high-resolution respirometry as a method and concept. | |||
:::: Today, such high resolution meets rapidly increasing demands, reflected by the increasing number and high level of [[O2k-Publications]]. Applications of the O2k in leading [[O2k-Network| O2k-Network Labs]] are the driving forces for a rapid advancement of mitochondrial physiology. Open access to the [[O2k-Manual]] is an integral part of the concept of [[OROBOROS INSTRUMENTS]] on [[Gentle Science]] and Scientific Social Responsibility. With sharing of [[O2k-Protocols]] and implementation of quality control, the study of mitochondrial and cell respiration emerges as a quantitative science. Thus high-resolution fluorespirometry provides the foundation for comparative mitochondrial physiology, biomedical research, translation into clinical applications, establishing quantitative functional mitochondrial diagnosis. | |||
::::* Sole source information: [[http://wiki.oroboros.at/images/a/a5/OROBOROS_SoleSource.pdf |'''pdf''']] | |||
::::* [[Oroboros FAQ|Frequently Asked Questions]] | |||
:::: The Oroboros O2k sets a world-wide standard in high-resolution respirometry - for mitochondrial studies in cell physiology and pathology. Developments progress [[beyond oxygen]]. | |||
:::: In contrast to merely offering an oxygen measuring device, the Oroboros O2k is integrated into a systems approach to high-resolution respirometry. This spans from experimental tools which are invaluable in the lab (e.g. [[ISS]]), or little aids that are crucial for tissue preparation (forceps), mitochondrial respration medium ([[MiR05-Kit]]), [[O2k-Protocols| substrate-uncoupler-inhibitor titration protocols]], and the unique [[O2k-Workshops]]. |
Revision as of 12:40, 9 July 2017
Oroboros O2k-technology
Description | Oroboros O2k - the modular system for high-resolution respirometry and fluorometry for mitochondria and cell research, using small amounts of biological samples. The O2k-Fluorometer is the basis for combined measurement of respiration and fluorometric signals, supported by the Titration-Injection microPump and DatLab 7 (new). The modular O2k-concept yields a high flexibility of fluorespirometry with add-on O2k-MultiSensor Modules for research in mitochondrial physiology and pathology. |
---|---|
Product ID | 1#### |
Type | O2k, Catalogue |
Link | Oroboros O2k-Catalogue, Gnaiger_2008_POS. MiPNet06.01 O2k-Overview |
Image |
- » Keywords
- » Top 10 Reasons for OROBOROS INSTRUMENTS
- » Quality and economy: high-output, low running costs
- » 2-chamber high-resolution respirometer
- » High-resolution respirometry
- » O2k-Dissection Set
- » Microbalance
- » O2k-Core
- » O2k-Fluorometer
- » O2k-MultiSensor Modules
- » PBI-Shredder O2k-Set
- » TIP2k-Module
O2k-Catalogue: O2k-Modules and Auxiliary Accessories
Oroboros O2k: development and distribution
- The O2k is developed by OROBOROS INSTRUMENTS in cooperation with WGT and produced in Austria by WGT.
- O2k-Fluorometry: HRR combined with measurement of ROS production, mitochondrial membrane potential, ATP production, Ca2+, pH.
- Titration-Injection microPump TIP2k: The O2k-TIP2k combination allows operation with programmable titration regimes, feedback control, and at steady-states, yielding a new flexibility in experimental design by combining the technical advantages of closed and open systems approaches. Automatic titrations for kinetics; feedback-controlled instrumental background tests; respiration in a closed or open system, in aerobic-anaerobic transitions or at steady-state oxygen concentrations, covering the entire physiological oxygen range and the extremes of hypoxia.
- O2k-Modules can be combined in any configuration with the O2k-Fluorometer, and can be added any time to extend the O2k according to application-specific objectives: potentiometry (pH, TPP+ or TPMP+ for mitochondrial membrane potential, Ca2+) and amperometry (NO, H2S).
- Auxiliary O2k-Tools provide extended support in the context of high-resolution respirometry and O2k-Fluorometry: Microsyringes, PBI-Shredder O2k-Set for tissue preparation; Microbalance, O2k-Dissection Set.
An Integrated Systems Approach: High-resolution fluorespirometry
- The Oroboros O2k is the unique state-of-the-art instrument for HRFR - where high-resolution counts:
- with small amounts of cells or tissue,
- at low respiratory activities,
- multiple substrate-uncoupler-inhibitor titrations (SUIT protocols) with resolution of changes in oxygen flux over prolonged incubation times,
- flexibility of O2k-MultiSensor applications,
- fast transitions at low oxygen levels,
- stability and quality control.
- High-resolution respirometry is the result of our long-term expertise in instrumental design, software development, mitochondrial physiology, clinical and pharmacological applications, and world-wide scientific cooperation. The innovative O2k-developments provide a system of highest quality and distinguish high-resolution respirometry as a method and concept.
- Today, such high resolution meets rapidly increasing demands, reflected by the increasing number and high level of O2k-Publications. Applications of the O2k in leading O2k-Network Labs are the driving forces for a rapid advancement of mitochondrial physiology. Open access to the O2k-Manual is an integral part of the concept of OROBOROS INSTRUMENTS on Gentle Science and Scientific Social Responsibility. With sharing of O2k-Protocols and implementation of quality control, the study of mitochondrial and cell respiration emerges as a quantitative science. Thus high-resolution fluorespirometry provides the foundation for comparative mitochondrial physiology, biomedical research, translation into clinical applications, establishing quantitative functional mitochondrial diagnosis.
- Sole source information: [|pdf]
- Frequently Asked Questions
- The Oroboros O2k sets a world-wide standard in high-resolution respirometry - for mitochondrial studies in cell physiology and pathology. Developments progress beyond oxygen.
- In contrast to merely offering an oxygen measuring device, the Oroboros O2k is integrated into a systems approach to high-resolution respirometry. This spans from experimental tools which are invaluable in the lab (e.g. ISS), or little aids that are crucial for tissue preparation (forceps), mitochondrial respration medium (MiR05-Kit), substrate-uncoupler-inhibitor titration protocols, and the unique O2k-Workshops.