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MitoFit-Reference sample
- The project MitoFit highlights the benefits of mitochondrial fitness.
Reference sample: cryopreserved mitochondria - development for functional control assays
- Abstract: A laboratory quality control system requires proficiency tests (MitoFit-PT) based on repeated assays (QCS) of a reference sample (WP4). Appropriate reference samples are not available for mitochondrial respiratory research. According to the EUROPEAN COMMISSION, a reference sample is “a sample of a batch of starting material, packaging material or finished product which is stored for the purpose of being analysed should the need arise during the shelf life of the batch concerned. ... Reference samples are for the purpose of analysis and, therefore, should be conveniently available to a laboratory with validated methodology.” In the present context, this implies that in order to serve as a respirometric refrence sample, the reference bulk material must be produced in sufficiently large quantities and homogeneity for splitting into subsamples. Vial to vial variability must be negligible relative to the error of diagnostic assays, to avoid sample variability as a confounding factor in the detection of systemic errors of measurement. Reference samples should retain preserved properties during transport and storage for prolonged periods of time, such that a single batch will last at least for one year. The reference sample should approximate the nature of biomedical and patient specimens with respect to experimental SUIT protocols to be applied.
