Difference between revisions of "MiPNet25.03 IOC144 Innsbruck AT"
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{{Publication | {{Publication | ||
|title=[[Image:OROBOROS-solo.png|right|60px|link=OROBOROS INSTRUMENTS]] | |title=[[Image:OROBOROS-solo.png|right|60px|link=OROBOROS INSTRUMENTS]] | ||
'''Innsbruck AT''', 2020 Jan 20-22. | '''Innsbruck AT''', 2020 Jan 20-22. O2k-Coaching Days - Basic '''IOC144'''. | ||
|authors=Oroboros | |authors=Oroboros | ||
|year=2020-01-20 | |year=2020-01-20 | ||
|journal=Mitochondr Physiol Network | |journal=Mitochondr Physiol Network | ||
|abstract=[[Image:O2k-Workshops.png|left|130px|link=O2k-Workshops]] | |abstract=[[Image:O2k-Workshops.png|left|130px|link=O2k-Workshops]] | ||
''' | '''O2k-Coaching Days - Basic'''. Innsbruck, Austria; 2020 Jan 20-22. | ||
|mipnetlab=AT_Innsbruck_Oroboros, AT_Innsbruck_MitoFit | |mipnetlab=AT_Innsbruck_Oroboros, AT_Innsbruck_MitoFit | ||
}} | }} | ||
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:::: ''' The course is fully booked. In case of cancellations further registrations can be accepted. In order to be added to the waiting list, send an email to [mailto:[email protected] [email protected]]''' | :::: ''' The course is fully booked. In case of cancellations further registrations can be accepted. In order to be added to the waiting list, send an email to [mailto:[email protected] [email protected]]''' | ||
:::: ''' | :::: '''O2k-Coaching Days - Basic''' is a training course which provides a fundamental introduction to high-resolution respirometry (HRR) with the Oroboros O2k. We will give you an overview of the O2k-FluoRespirometer, including real-time analysis with DatLab 7.4 and applications of the Titration-Injection microPump TIP2k. Hands-on sessions range from OroboPOS technical service (instrumental quality control system which is an essential component of HRR) to respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols. Also the new Oroboros SUITbrowser will be presented. The SUITbrowser helps you to find the best SUIT protocol for your specific research questions. Many optimized SUIT protocols are available as DL-Protocols and will be shown at the O2k-Coaching Days. | ||
== Venue == | == Venue == | ||
::::» Oroboros | ::::» Oroboros O2k-Laboratory | ||
::::: Schoepfstrasse 18, 6020 Innsbruck | ::::: Schoepfstrasse 18, 6020 Innsbruck | ||
::::» Information on travel and venue: '''[[IOC Innsbruck]]'''. | ::::» Information on travel and venue: '''[[IOC Innsbruck]]'''. | ||
::::» Hotel recommendation: '''Basic Hotel Innsbruck***'''(https://www.basic-hotel.at/en/) | ::::» Hotel recommendation: '''Basic Hotel Innsbruck***'''(https://www.basic-hotel.at/en/) | ||
::::: Using code "''OROBOROS''" for booking online you will get the Single Room for € 85.00 and the Double Room for € 120.00 including breakfast. Rooms are available at a first come first served basis. | ::::: Using code "''OROBOROS''" for booking online you will get the Single Room for € 85.00 and the Double Room for € 120.00 including breakfast. Rooms are available at a first come first served basis. | ||
::::: Free cancellation until 7 days prior. Booking requires a credit card for guarantee. Payment on site. A | ::::: Free cancellation until 7 days prior. Booking requires a credit card for guarantee. Payment on site. A 2 € City tax to be charged additionally. | ||
== Registration == | == Registration == | ||
<br> | <br> | ||
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|08:15 | |08:15 | ||
|Welcome - Get-together: Introduction of participants and their research interests | |Welcome - Get-together: Introduction of participants and their research interests | ||
|Oroboros | |Oroboros O2k-Laboratory | ||
|- | |- | ||
|08:30 | |08:30 | ||
|OroboPOS service and O2k instrumental setup - overview with videoclips | |OroboPOS service and O2k instrumental setup - overview with videoclips | ||
|Oroboros | |Oroboros O2k-Laboratory | ||
|- | |- | ||
|09:15 | |09:15 | ||
|'''Hands-on''': OroboPOS service and O2k instrumental setup | |'''Hands-on''': OroboPOS service and O2k instrumental setup | ||
|Oroboros | |Oroboros O2k-Laboratory | ||
|- | |- | ||
|09:55 | |09:55 | ||
|DatLab 7.4 overview | |DatLab 7.4 overview | ||
|Oroboros | |Oroboros O2k-Laboratory | ||
|- | |- | ||
|10:30 | |10:30 | ||
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|11:00 | |11:00 | ||
|Instrumental quality control 1: oxygen calibration | |Instrumental quality control 1: oxygen calibration | ||
|Oroboros | |Oroboros O2k-Laboratory | ||
|- | |- | ||
|11:30 | |11:30 | ||
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DL-Protocol: O2 calibration air | DL-Protocol: O2 calibration air | ||
|Oroboros | |Oroboros O2k-Laboratory | ||
|- | |- | ||
|12:00 | |12:00 | ||
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|'''Hands-on''': Instrumental quality control 1: oxygen calibration (continuation) | |'''Hands-on''': Instrumental quality control 1: oxygen calibration (continuation) | ||
DL-Protocol: O2 calibration air | DL-Protocol: O2 calibration air | ||
|Oroboros | |Oroboros O2k-Laboratory | ||
|- | |- | ||
|13:30 | |13:30 | ||
|Instrumental quality control 2: Instrumental O<sub>2</sub> background – overview with videoclips | |Instrumental quality control 2: Instrumental O<sub>2</sub> background – overview with videoclips | ||
|Oroboros | |Oroboros O2k-Laboratory | ||
|- | |- | ||
|14:00 | |14:00 | ||
|'''Hands-on''':Instrumental quality control 2: Instrumental O<sub>2</sub> background | |'''Hands-on''':Instrumental quality control 2: Instrumental O<sub>2</sub> background | ||
DL-Protocol: Instrumental O2 background TiP2k | DL-Protocol: Instrumental O2 background TiP2k | ||
|Oroboros | |Oroboros O2k-Laboratory | ||
|- | |- | ||
|15:00 | |15:00 | ||
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|15:30-17:30 | |15:30-17:30 | ||
|'''Hands-on''': Instrumental quality control 1 and 2. DatLab analysis. | |'''Hands-on''': Instrumental quality control 1 and 2. DatLab analysis. | ||
|Oroboros | |Oroboros O2k-Laboratory | ||
|- | |- | ||
|} | |} | ||
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DL-Protocol: O2 calibration air | DL-Protocol: O2 calibration air | ||
|Oroboros | |Oroboros O2k-Laboratory | ||
|- | |- | ||
|09:15 | |09:15 | ||
|Introduction to substrate-uncoupler-inhibitor titration (SUIT) protocols – fundamental principles. SUIT reference protocol: RP1&RP2 | |Introduction to substrate-uncoupler-inhibitor titration (SUIT) protocols – fundamental principles. SUIT reference protocol: RP1&RP2 | ||
|Oroboros | |Oroboros O2k Laboratory | ||
|- | |- | ||
|09:45 | |09:45 | ||
|SUITbrowser: how to find the best SUIT protocol for your research questions. | |SUITbrowser: how to find the best SUIT protocol for your research questions. | ||
|Oroboros | |Oroboros O2k Laboratory | ||
|- | |- | ||
|10:00 | |10:00 | ||
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DL-Protocol: SUIT-002 O2 ce-pce D007.DLP | DL-Protocol: SUIT-002 O2 ce-pce D007.DLP | ||
|Oroboros | |Oroboros O2k Laboratory | ||
|- | |- | ||
|11:45 | |11:45 | ||
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DL-Protocol: O2k-cleaning AfterUse | DL-Protocol: O2k-cleaning AfterUse | ||
|Oroboros | |Oroboros O2k-Laboratory | ||
|- | |- | ||
|12:00 | |12:00 | ||
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DL-Protocol: O2k-cleaning AfterUse | DL-Protocol: O2k-cleaning AfterUse | ||
|Oroboros | |Oroboros O2k-Laboratory | ||
|- | |- | ||
|13:30 | |13:30 | ||
|DatLab 7.4 analysis: Introduction and new features | |DatLab 7.4 analysis: Introduction and new features | ||
|Oroboros | |Oroboros O2k-Laboratory | ||
|- | |- | ||
|15:00 | |15:00 | ||
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|15:30-17:30 | |15:30-17:30 | ||
|Individual DatLab analysis + summary | |Individual DatLab analysis + summary | ||
|Oroboros | |Oroboros O2k-Laboratory | ||
|- | |- | ||
|} | |} | ||
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DL-Protocol: O2 calibration air | DL-Protocol: O2 calibration air | ||
|Oroboros | |Oroboros O2k-Laboratory | ||
|- | |- | ||
|10:00 | |10:00 | ||
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DL-Protocol: SUIT-002 O2 ce-pce D007.DLP | DL-Protocol: SUIT-002 O2 ce-pce D007.DLP | ||
|Oroboros | |Oroboros O2k-Laboratory | ||
|- | |- | ||
|11:45 | |11:45 | ||
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DL-Protocol: O2k-cleaning AfterUse | DL-Protocol: O2k-cleaning AfterUse | ||
|Oroboros | |Oroboros O2k-Laboratory | ||
|- | |- | ||
|12:00 | |12:00 | ||
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DL-Protocol: O2k-cleaning AfterUse | DL-Protocol: O2k-cleaning AfterUse | ||
|Oroboros | |Oroboros O2k-Laboratory | ||
|- | |- | ||
|13:15 | |13:15 | ||
|DatLab analysis | |DatLab analysis | ||
|Oroboros | |Oroboros O2k-Laboratory | ||
|- | |- | ||
|14:00 | |14:00 | ||
|O2k-Applications - overview | |O2k-Applications - overview | ||
|Oroboros | |Oroboros O2k-Laboratory | ||
|- | |- | ||
|14:30 | |14:30 | ||
|Proficiency test: DatLab analysis summary | |Proficiency test: DatLab analysis summary | ||
|Oroboros | |Oroboros O2k-Laboratory | ||
|- | |- | ||
|15:00 | |15:00 | ||
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[[Image:MitoGlobal.jpg|right|80px|link=MitoGlobal|MitoGlobal]] | [[Image:MitoGlobal.jpg|right|80px|link=MitoGlobal|MitoGlobal]] | ||
O2k-Workshops and | O2k-Workshops and O2k-Coaching Days are listed as [[MitoGlobal Events]]. | ||
{{Labeling | {{Labeling |
Revision as of 03:27, 19 November 2019
MiPNet25.03 IOC144 Innsbruck AT
Innsbruck AT, 2020 Jan 20-22. O2k-Coaching Days - Basic IOC144. |
Oroboros (2020-01-20) Mitochondr Physiol Network
Abstract:
O2k-Coaching Days - Basic. Innsbruck, Austria; 2020 Jan 20-22.
• O2k-Network Lab: AT_Innsbruck_Oroboros, AT_Innsbruck_MitoFit
General information
- The course is fully booked. In case of cancellations further registrations can be accepted. In order to be added to the waiting list, send an email to [email protected]
- O2k-Coaching Days - Basic is a training course which provides a fundamental introduction to high-resolution respirometry (HRR) with the Oroboros O2k. We will give you an overview of the O2k-FluoRespirometer, including real-time analysis with DatLab 7.4 and applications of the Titration-Injection microPump TIP2k. Hands-on sessions range from OroboPOS technical service (instrumental quality control system which is an essential component of HRR) to respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols. Also the new Oroboros SUITbrowser will be presented. The SUITbrowser helps you to find the best SUIT protocol for your specific research questions. Many optimized SUIT protocols are available as DL-Protocols and will be shown at the O2k-Coaching Days.
Venue
- » Oroboros O2k-Laboratory
- Schoepfstrasse 18, 6020 Innsbruck
- » Information on travel and venue: IOC Innsbruck.
- » Hotel recommendation: Basic Hotel Innsbruck***(https://www.basic-hotel.at/en/)
- Using code "OROBOROS" for booking online you will get the Single Room for € 85.00 and the Double Room for € 120.00 including breakfast. Rooms are available at a first come first served basis.
- Free cancellation until 7 days prior. Booking requires a credit card for guarantee. Payment on site. A 2 € City tax to be charged additionally.
- » Oroboros O2k-Laboratory
Registration
- Registration closed.
Tutors and lecturers
Luiza Cardoso, PhD., Oroboros Instruments - PostDoc
Marco Di Marcello, BSc., Oroboros Instruments - Scientific Assistant
Carolina Doerrier, PhD. - Chief scientific officer
Luiz Felipe Garcia-Souza, PhD. student, Oroboros Instruments
Erich Gnaiger, PhD., Oroboros Instruments, CEO
Preliminary programme
Monday 20th
Topic Room 08:15 Welcome - Get-together: Introduction of participants and their research interests Oroboros O2k-Laboratory 08:30 OroboPOS service and O2k instrumental setup - overview with videoclips Oroboros O2k-Laboratory 09:15 Hands-on: OroboPOS service and O2k instrumental setup Oroboros O2k-Laboratory 09:55 DatLab 7.4 overview Oroboros O2k-Laboratory 10:30 Coffee 11:00 Instrumental quality control 1: oxygen calibration Oroboros O2k-Laboratory 11:30 Hands-on: Instrumental quality control 1: oxygen calibration DL-Protocol: O2k-cleaning BeforeUse
DL-Protocol: O2 calibration air
Oroboros O2k-Laboratory 12:00 Lunch break 13:00 Hands-on: Instrumental quality control 1: oxygen calibration (continuation) DL-Protocol: O2 calibration air
Oroboros O2k-Laboratory 13:30 Instrumental quality control 2: Instrumental O2 background – overview with videoclips Oroboros O2k-Laboratory 14:00 Hands-on:Instrumental quality control 2: Instrumental O2 background DL-Protocol: Instrumental O2 background TiP2k
Oroboros O2k-Laboratory 15:00 Coffee 15:30-17:30 Hands-on: Instrumental quality control 1 and 2. DatLab analysis. Oroboros O2k-Laboratory
Tuesday 21st
Topic Room 08:30 Hands-on: Instrumental quality control 1: oxygen calibration DL-Protocol: O2k-cleaning BeforeUse
DL-Protocol: O2 calibration air
Oroboros O2k-Laboratory 09:15 Introduction to substrate-uncoupler-inhibitor titration (SUIT) protocols – fundamental principles. SUIT reference protocol: RP1&RP2 Oroboros O2k Laboratory 09:45 SUITbrowser: how to find the best SUIT protocol for your research questions. Oroboros O2k Laboratory 10:00 Coffee 10:30 Hands-on: O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with the reference protocol RP1 (SUIT-001) and RP2 (SUIT-002). DL-Protocol: SUIT-001 O2 ce-pce D003.DLP
DL-Protocol: SUIT-002 O2 ce-pce D007.DLP
Oroboros O2k Laboratory 11:45 Hands-on: O2k-cleaning after use DL-Protocol: O2k-cleaning AfterUse
Oroboros O2k-Laboratory 12:00 Lunch break 13:00 Hands-on: O2k-cleaning after use (continuation) DL-Protocol: O2k-cleaning AfterUse
Oroboros O2k-Laboratory 13:30 DatLab 7.4 analysis: Introduction and new features Oroboros O2k-Laboratory 15:00 Coffee 15:30-17:30 Individual DatLab analysis + summary Oroboros O2k-Laboratory
Wednesday 22nd
Topic Room 08:30 MitoFit proficiency test Hands-on: Instrumental quality control 1: oxygen calibration
DL-Protocol: O2k-cleaning BeforeUse
DL-Protocol: O2 calibration air
Oroboros O2k-Laboratory 10:00 Coffee 10:30 MitoFit proficiency test Hands-on: O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with the reference protocol RP1 (SUIT-001) and RP2 (SUIT-002).
DL-Protocol: SUIT-001 O2 ce-pce D003.DLP
DL-Protocol: SUIT-002 O2 ce-pce D007.DLP
Oroboros O2k-Laboratory 11:45 Hands-on: O2k-cleaning after use DL-Protocol: O2k-cleaning AfterUse
Oroboros O2k-Laboratory 12:00 Lunch break 13:00 Hands-on: O2k-cleaning after use (continuation) DL-Protocol: O2k-cleaning AfterUse
Oroboros O2k-Laboratory 13:15 DatLab analysis Oroboros O2k-Laboratory 14:00 O2k-Applications - overview Oroboros O2k-Laboratory 14:30 Proficiency test: DatLab analysis summary Oroboros O2k-Laboratory 15:00 Coffee 15:30 The Bioblast wiki, MitoFit Preprint Archives, O2k-Network and MitoEAGLE MiPArt 16:30 Feedback & conclusions MiPArt 17:00 Farewell activity MiPArt
Participants
Participant | Institution | |
---|---|---|
Buoso Chiara | AT Innsbruck Weiss G - Medical University Innsbruck, Innsbruck (AT) | |
Carter Kristen | US MS Jackson Cornelius DC - University of Mississippi, Jackson (US) | |
Chamkha Imen | SE Lund Elmer E - Lund University, Lund (SE) | |
Coldewey Sina | DE Jena Coldewey S - Jena University Hospital, Jena (DE) | |
Esper Treml Ricardo | DE Jena Coldewey S - Jena University Hospital, Jena (DE) | |
Gayathri Narayanappa | IN Bangalore Gayathri N - NIMHANS, Bangalore (IN) | |
Griffiths Kate | UK Glasgow Selman C - Glasgow University, Glasgow (UK) | |
Hussein Rawya | SE Lund Elmer E - Lund University, Lund (SE) | |
Neu Charles | DE Jena Coldewey S - Jena University Hospital, Jena (DE) | |
Place Nicolas | CH Lausanne Place N - Université de Lausanne, Lausanne (CH) | |
Participant | SE Lund Elmer E - Lund University, Lund (SE) |
Contact
- Oroboros Instruments GmbH
- High-Resolution Respirometry
- Schoepfstrasse 18
- A-6020 Innsbruck, Austria
- Tel: +43 512 566796
- Fax: +43 512 566796 20
- E-Mail: [email protected]
- Mitochondria and Cell Research
IOC recommended reading
O2k-Workshops and O2k-Coaching Days are listed as MitoGlobal Events.
Labels:
ORO, IOC, MitoGlobal, Next, 2020