MiPNet23.11 IOC137 Innsbruck AT
Innsbruck AT, 2018 Dec 10-12. MitoFit Coaching Days - Basic IOC137. |
Oroboros (2018-12-10) Mitochondr Physiol Network
Abstract:
MitoFit Coaching Days - Basic. Innsbruck, Austria; 2018 Dec 10-12.
β’ O2k-Network Lab: AT_Innsbruck_Oroboros, AT_Innsbruck_MitoFit
General information
- MitoFit Coaching Days - Basic provide a fundamental introduction to High-Resolution FluoRespirometry with the Oroboros O2k. Hands-on sessions range from OroboPOS technical service to respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols.
- Β» Information on travel and venue: IOC Innsbruck.
- Please bring your labcoat with you. Thank you!
Registration
- closed
Tutors, lecturers, and managers
Manuela Passrugger, BSc., Oroboros Instruments - Biomedical Assistant
Marco di Marcello, BSc., Medical University of Innsbruck, MitoFit project - Assistant
Ana Carolina Bastos Sant'Anna Silva, MSc., Oroboros Instruments - PhD. student TRANSMIT
Garcia e Souza Luiz Felipe, MSc., Leopold-Franzens-University - PhD. student
Carolina Doerrier, PhD., Oroboros Instruments - Chief scientific officer
Erich Gnaiger, PhD., Oroboros Instruments - CEO
Programme
Monday 10th Topic Room 08:15 Welcome - Get-together: Introduction of participants and their research interests ORO-MitoFit Lab 08:30 OroboPOS service and O2k instrumental setup - overview with videoclips ORO-MitoFit Lab 09:30 Hands-on: OroboPOS service and O2k instrumental setup ORO-MitoFit Lab 10:10 Hands-on: DatLab 7 ORO-MitoFit Lab 10:30 Coffee 11:00 Instrumental quality control 1: oxygen calibration ORO-MitoFit Lab 11:30 Hands-on: Instrumental quality control 1: oxygen calibration DL-Protocol: O2k-cleaning before use
DL-Protocol: O2 calibration air
ORO-MitoFit Lab 12:00 Lunch break 13:00 Hands-on:Instrumental quality control 1: oxygen calibration (continuation) DL-Protocol: O2 calibration air
ORO-MitoFit Lab 13:30 Instrumental quality control 2: Instrumental O2 background β overview with videoclips ORO-MitoFit Lab 14:00 Hands-on:Instrumental quality control 2: Instrumental O2 background DL-Protocol: Instrumental O2 background TiP2k
ORO-MitoFit Lab 15:00 Coffee 15:30-18:00 Hands-on: Instrumental quality control 1 and 2. DatLab analysis. ORO-MitoFit Lab
Tuesday 11th Topic Room 09:00 Hands-on: Instrumental quality control 1: oxygen calibration DL-Protocol: O2k-cleaning before use
DL-Protocol: O2 calibration air
ORO-MitoFit Lab 09:15 Application of the O2k Series H ORO-MitoFit Lab 09:30 Introduction to substrate-uncoupler-inhibitor titration (SUIT) protocols β fundamental principles. SUIT reference protocol: RP1&RP2 ORO-MitoFit Lab 10:00 Coffee 10:30 Hands-on: O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2. DL-Protocol: SUIT-1_O2_pce_D03.DLP
DL-Protocol: SUIT-2 O2 pce D07.DLP
ORO-MitoFit Lab 11:45 Hands-on: O2k-cleaning after use DL-Protocol: O2k-cleaning after use
ORO-MitoFit Lab 12:00 Lunch break 13:00 Hands-on: O2k-cleaning after use DL-Protocol: O2k-cleaning after use
ORO-MitoFit Lab 13:30 Introduction to DatLab analysis ORO-MitoFit Lab 15:00 Coffee 15:30-18:00 Individual DatLab analysis + summary ORO-MitoFit Lab
Wednesday 12th Topic Room 09:00 MitoFit proficiency test: Hands-on: Instrumental quality control 1: oxygen calibration
DL-Protocol: O2k-cleaning before use
DL-Protocol: O2 calibration air
ORO-MitoFit Lab 10:00 Coffee 10:30 MitoFit proficiency test: Hands-on: O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2.
DL-Protocol: SUIT-1_O2_pce_D03.DLP
DL-Protocol: SUIT-2 O2 pce D07.DLP
ORO-MitoFit Lab 11:45 Hands-on: O2k-cleaning after use DL-Protocol: O2k-cleaning after use
ORO-MitoFit Lab 12:00 Lunch break 13:00 Hands-on: O2k-cleaning after use DL-Protocol: O2k-cleaning after use
ORO-MitoFit Lab 13:15 DatLab analysis ORO-MitoFit Lab 14:00 DatLab analysis summary ORO-MitoFit Lab 15:00 Coffee 15:30 The Bioblast wiki, O2k-Network and MITOEAGLE MiPArt 16:15 Feedback & conclusions MiPArt 17:00 Farewell activity MiPArt
Participants
Participant | Institution | |
---|---|---|
Barlow Jonathan | UK Birmingham Lai YC - University of Birmingham, UK | |
Chakrabarti Lisa | UK Nottingham Chakrabarti L - University of Nottingham, UK | |
Gnaiger Carolina | AT Innsbruck Oroboros, Innsbruck, AT | |
Ho Dieu Hien | DE Cologne Trifunovic A, Institute of Mitocondiral Disease and Ageing, Cologne, DE | |
Jedlicka Jan | CZ Pilsen Kuncova J, Charles University, Plzne, CZ | |
Kapferer Werner | AT Innsbruck Jansen-Duerr P - University of Innsbruck, AT | |
Kranewitter Sabine | AT Innsbruck Oroboros, Innsbruck, AT | |
Maciej Sarah | DE Cologne Trifunovic A, Institute of Mitocondiral Disease and Ageing, Cologne, DE | |
Maseko Tumisang Edward | CZ Hradec Kralove Cervinkova Z, Charles University, Hradec Kralove, CZ | |
Oliviera Ben | UK Leicester Watson E, Kings College Hospital, London, UK | |
Saharnaz Sarlak | FR Bordeaux Rossignol R - University of Bordeaux, Bordeaux, FR | |
Thapa Maheshwor | AT Salzburg Sperl W - Biocrates Life Sciences AG, Innsbruck, AT | |
Ton Riccardo | AU Sydney Griffith SC - Macquarie University, Sydney, AU | |
Zdrazilova Lucie | CZ Prague Zeman J - Charles University, Prague, CZ |
Contact
- Oroboros Instruments
- High-Resolution FluoRespirometry
- SchΓΆpfstrasse 18
- A-6020 Innsbruck, Austria
- Tel: +43 512 566796
- Fax: +43 512 566796 20
- Mitochondria and cell research
Project support
IOC recommended reading
O2k-Workshops and MitoFit Coaching Days are listed as MitoGlobal Events.
Labels:
ORO, IOC, 2018, MitoFit, TRANSMIT, Next, MitoGlobal