Revision as of 18:23, 28 August 2015

high-resolution terminology - matching measurements at high-resolution

Glycerophosphate

Description

Glycerophosphate (synonym: α-glycerophosphate; glycerol-3-phosphate; C₃H₉O₆P) is an organophosphate and it is a component of glycerophospholipids. The mitochondrial Glycerophosphate dehydrogenase complex oxidizes glycerophosphate to dihydroxyacetone phosphate and feeds electrons directly to ubiquinone.

Abbreviation: Gp

MitoPedia topics: Substrate and metabolite

Nomenclature

Due to an ambiguity in the D,L nomenclature based on the Fisher projection the biologically active enantiomer can be labeled either D-glycerol 1-phosphate or L-glycerol 3-phosphate. Alternatives are sn-glycerol 3-phosphate or DL-alpha-glycerophosphate. Following the Cahn–Ingold–Prelog priority rules, the biologically active substance is always the (R) enantiomer, independent of whether the substance is called glycerol 1-phosphate or glycerol 3-phosphate.

Source

Recommendation by Chuck Hoppel, Director, Ctr Mitochondrial Diseases; Professor, Dept Pharmacology, Case Western Reserve Univ, Cleveland, Ohio, US - US OH Cleveland Hoppel CL.

94124 Sigma sn-Glycerol 3-phosphate lithium salt, ≥95.0% (TLC) Synonym: D-Glycerol 1-phosphate lithium salt, L-Glycerol 3-phosphate lithium salt. MSDS. CAS Number 17989-41-2 (free acid) Empirical Formula (Hill Notation) C₃H₉O₆P · xLi+, Molecular Weight 172.07 (free acid basis).

Application in HRR

1 M stock solution, final concentration in the O2k-Chamber is 10 mM.

If a racemic mixture (like Sigma G-2138) is replaced by the biologically active enantiomer the amount of substance (measured in moles) usually has to be halved. However, also consider that stated purities may vary, requiring further adjustments.

List of publications: CGpDH-linked substrate state

@@ Line 15: / Line 15: @@
 == Source ==
-:* [http://www.sigmaaldrich.com/catalog/search?term=94124&interface=All&N=0&mode=match%20partialmax&lang=en&region=LV&focus=product 94124 Sigma sn-Glycerol 3-phosphate lithium salt], ≥95.0% (TLC) Synonym: D-Glycerol 1-phosphate lithium salt, L-Glycerol 3-phosphate lithium salt. MSDS. CAS Number 17989-41-2 (free acid) Empirical Formula (Hill Notation) C<sub>3</sub>H<sub>9</sub>O<sub>6</sub>P · xLi+,  Molecular Weight 172.07 (free acid basis). This is a recommendation by [[Hoppel CL |Chuck Hoppel]], Director, Ctr Mitochondrial Diseases; Professor, Dept Pharmacology, Case Western Reserve Univ, Cleveland, Ohio, US - [[US OH Cleveland Hoppel CL]].
+Recommendation by [[Hoppel CL |Chuck Hoppel]], Director, Ctr Mitochondrial Diseases; Professor, Dept Pharmacology, Case Western Reserve Univ, Cleveland, Ohio, US - [[US OH Cleveland Hoppel CL]].
+:* [http://www.sigmaaldrich.com/catalog/search?term=94124&interface=All&N=0&mode=match%20partialmax&lang=en&region=LV&focus=product 94124 Sigma sn-Glycerol 3-phosphate lithium salt], ≥95.0% (TLC) Synonym: D-Glycerol 1-phosphate lithium salt, L-Glycerol 3-phosphate lithium salt. MSDS. CAS Number 17989-41-2 (free acid) Empirical Formula (Hill Notation) C<sub>3</sub>H<sub>9</sub>O<sub>6</sub>P · xLi+,  Molecular Weight 172.07 (free acid basis).
-:* 1 M stock solution, final concentration in the O2k-Chamber is 10 mM. If a racemic mixture (like Sigma G-2138) is replaced by the biologically active enantiomer the amount of substance (measured in moles) usually has to be halved. However, also consider that stated purities may vary, requiring further adjustments.
+== Application in HRR ==
+:* 1 M stock solution, final concentration in the O2k-Chamber is 10 mM.
+If a racemic mixture (like Sigma G-2138) is replaced by the biologically active enantiomer the amount of substance (measured in moles) usually has to be halved. However, also consider that stated purities may vary, requiring further adjustments.