Difference between revisions of "Antimycin A"
From Bioblast
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== Compare == | == Compare == | ||
* [[Myxothiazol]] - this CIII inhibitor induces less ROS production but is more expensive than Ama. | * [[Myxothiazol]] - this CIII inhibitor induces less ROS production but is more expensive than Ama. | ||
== Antimycin A and ROS production == | |||
* Is hydrogen peroxide production measured after inhibition of the electron transfer system by rotenone and antimycin A of diagnostic significance? - Please, share your evidence (Email to [email protected]). |
Revision as of 09:03, 12 October 2015
Description
Antimycin A is an inhibitor of Complex III (CIII). It binds to the Qi site of CIII and inhibits the transfer of electrons from heme bH to oxidized Q (Qi site inhibitor).
Abbreviation: Ama
Reference: MiPNet09.12
MitoPedia topics: Inhibitor
Applications in HRR
Ama: Antimycin A (from Streptomyces sp.) Sigma A 8674, 25 mg, store at -20 °C: FW = 540
Caution: Very toxic. Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
Preparation of 5 mM stock solution (dissolved in 99.9% ethanol):
- Weigh 5.4 mg of antimycin A into a small glass vial. Caution: Ama is hygroscopic.
- Add 2 ml ethanol.
- Divide into 0.2 ml portions in glass vials.
- Store at -20 °C.
O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: 1 µl using a 10 µl syringe (2 ml O2k-chamber).
- Final concentration: 2.5 µM.
Compare
- Myxothiazol - this CIII inhibitor induces less ROS production but is more expensive than Ama.
Antimycin A and ROS production
- Is hydrogen peroxide production measured after inhibition of the electron transfer system by rotenone and antimycin A of diagnostic significance? - Please, share your evidence (Email to [email protected]).