From Bioblast
Description
File:1PGM;2D;3U;3anoxia;4Myx.png
Reference: A: protocol for simultaneous determination of O2 flux and NADH autofluorescence in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells)- SUIT-006
SUIT number: D084_1PGM;2D;3U;3anoxia;4Myx
O2k-Application: NADH
Communicated by Doerrier C (last update 2022-05-03)
Representative traces
File:SUIT-006 NADH mt D084 O2.png File:SUIT-006 NADH mt D084.png
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
mt | REN | mt | ||
1PGM | PGML(n) | N | CI | 1PGM
|
2D | PGMP | N | CI | 1PGM;2D
|
(3Omy) | PGML(Omy) | N | CI | 1PGM;2D;(3Omy)
|
4U | PGME | N | CI | 1PGM;2D;(3Omy);4U
|
5Anox | N | CI | 1PGM;2D;(3Omy);4U;5Anox | |
6Myx | N | CI | 1PGM;2D;(3Omy);4U;5Anox;6Myx
| |
7Reox | ROX | 1PGM;2D;(3Omy);4U;5Anox;6Myx;7Reox |
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Pathway control
- Β» Electron transfer pathway
- Β» Fatty acid oxidation pathway control state, F
- Β» NADH electron transfer-pathway state, N
- Β» Succinate pathway control state, S
- Β» NS-pathway control state, NS
- Β» Glycerophosphate pathway control state, Gp
- Β» Complex IV single step, CIV
- Β» Anaplerotic pathway control state
- Pathway control
- Main fuel substrates
- Β» Glutamate, G
- Β» Glycerophosphate, Gp
- Β» Malate, M
- Β» Octanoylcarnitine, Oct
- Β» Pyruvate, P
- Β» Succinate, S
- Main fuel substrates
- Glossary
Strengths and limitations
Compare SUIT protocols
Chemicals and syringes
- Suggested stock concentrations are shown in the specific DL-Protocol.