X-mass Carol

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X-mass Carol

Gnaiger Erich (2020) A canonical carol on quantities, symbols, and units. MitoFit Preprint Arch 2020.4.v0.

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Abstract: Pre-preprint version 0 (v0.1) (2020-06-13) last update 2020-06-16

Humbug is a hot issue at a time of bugs and irreproducibility in science, fake news in social media, and a sense of mistrust in the solutions of the COVID 19 crisis. Trust in science calls for a language that is understood. Some fundamental terms, symbols, and units at the basis of counting and measuring are presented as a Canonical Carol from medium to message to meaning, to crack the code for communication from physical and chemical sciences to molecular biology and mitochondrial physiology. The theme is: BAH! Humbug! The meaning of BAH! will be explained extempore, the retrograde and self-referential meaning of Humbug! is: back to Humbug ― the end and the beginning. • Keywords: Amount of substance, Bit, Body mass, Cell respiration, Buglish, Canonical and practical, Canonical ensemble, Concentration, Count, Dimension, Entity, Extroduction, Flow, Flux, Format, IUPAC, International System of Units, Isolated mitochondria, Living cells, Mass, Measure, MitoPedia, Molar mass, Normalization of rate, Object, Organism, Permeabilized cells, Publish, Sample, System, Unit, Volume

Extroduction

1. This article aims at providing a new understanding of the concepts of counting and measuring. Although we are familiar with the concepts of counting and measuring — or we are counting and measuring without the need to be familiar with these concepts —, established systems of quantities, symbols, and units do not sufficiently reflect the fact that quantities for measurement of size (mass [kg], volume [L]) are opposite to a group of quantities that are strictly linked to counting (count [x], amount [mol], charge [C]). The units 'mole' for amount and 'coulomb' for charge are connected to the unit 'counting-unit' [x] for count by fixed stoichiometries, but [x] is not part of the International System of Units (SI).
2. This article is relevant to all areas of science and philosophy and all disciplines that are involved with counting and measuring. However, for strictly Practical-Technical Realists R(PT), the Canonical Carol is too theoretical. For Technical-Theoretical Nerds N(TT), the Canonical Carol is too practical. If you identify yourself as a PTC-type of the Brave person B(PTC) — Practical&Technical-Theoretical&Canonical — then pop up to the PDF of the Canonical Carol.
3. The Canonical Carol is written in recursive form about recursive systems, where an experimental system is contained in an instrumental system, an extroduction introduces the introduction, and the quantity 'count' devours its own physicochemical unit x. Enjoy the Canonical Carol from beginning to end, the Carolical Canon from end to beginning, to realize that the beginning is not in the introduction, and the beginning is not in the Canonical Carol — the beginning is at a Higher-level H in the canon, and level H is the extroduction.

This extroduction is the external or executive introduction to the Canonical Carol.

• Push down to »Extroduction«

Extroductory Canons on quantities, symbols, and units. A composition composed in the spirit of Douglas Hofstadter

• Push down or pull up to »Hofstadter DR (1979) Gödel, Escher, Bach: An eternal golden braid. A metaphorical fugue on minds and machines in the spirit of Lewis Carroll. Harvester Press:499 pp.«

While there exists only one single Canonical Carol, there is a potential maximum number of Extroductory Canons set by the global number of counting human people or by the number of groups of counting human people. Babies can count before being taught to count and before talking, and the ability to count is not restricted to the human species. For each group of persons and species a different pattern triggers counting. This trigger-pattern should be encoded into each specific variation or Voice of Extroductory Canons. Such a large number of Extroductions cannot be included within the Canonical Carol, since it would blow up its word count beyond a reasonable number of words allowed in a scientific system of publication. The word-count expansion in the extroduction aims at introducing different PTC-audiences to the why should I open the box, and to the how to read it's contents. The Extroduction is the manual for the Canonical Carol written in different Voices, while the Canonical Carol is written in different Staves. As usual, this manual for the Canonical Carol cannot be well understood without reading the Canonical Carol, which should be used as a manual for the Extroductory Canons. As always, read the manual first.

Table of extroductory contents

Voice	Title
H	Historical stories, novels and philisophical bestsellers. BAH! Humbug!
P	Are you a Practical&Technical-Theoretical&Canonical person who can switch to PTC-mood?
X	Taking part in an X-mass party
B	To drink or not to drink Beer
O	A Canonical O2k-Manual for high-resolution respirometry: why the living cells died and dyed
M	MitoEAGLE states and rates — a coral on canonical and practical
C	A puzzle on pine Cones

H: Historical stories, novels and philisophical bestsellers. BAH! Humbug!

Figure H 1. Thomas Rowlandson Humbugging, or raising the Devil (1800), illustrating Charles Dickens and L. Frank Baum’s Bah! Humbug! - Source

Figure H 2. The wonderful wizard of OZ.

Figure H 3. Lewis Carroll Through the looking glass (1871)

Figure H 4. Lewis Carroll's Lewis Carrols‘ Jabberwocky

Figure H 5. Maurits Cornelis Escher Dragon (1952) – the Ouroboros

Figure H 6. The medium is the massage (1967)

BAH! Humbug! ― is the theme of the canonical carol on terminology that found its roots in Charls Dickens novella A Christmas Carol (1843) (Figure 1) and L Frank Baum’s The Wonderful Wizard of Oz (Figure 2). But first of all BAH is inspired by a canonical Carroll: Lewis Carroll’s novel Through the Looking-Glass (Figures 3 and 4), the sequel to Alice's Adventures in Wonderland (1865) referred to by Douglas Richard Hofstadter in his book Gödel, Escher, Bach: An eternal golden braid. A metaphorical fugue on minds and machines in the spirit of Lewis Carroll (1979). MC Escher’s Dragon ― the Ouroboros (Figure 5) ― is a canonical self-referential symbol that penetrates BAH from beginning to end and from the end to the beginning: when the Highest-level system H devours itself and becomes its own Bit B, and backwards when Bit B creates its own system B, when the object Body B inflates into its own system Body B ― when the medium is the message. The book The Medium is the Massage (1967) was co-created by Herbert Marshall McLuhan and Quentin Fiore (Figure 6).

In practical language we cannot but use dualistic terminology. Does a term describe the object of our investigation? Or is the term an object, worthwhile of canonical investigation? Extroductory Canon Voices B-major and B-minor are all about dualistic terminology, with a different twist. But first, how do you see yourself as an object? Are you one of the 666 coauthors of 'Mitochondrial physiology' (BEC 2020.1)?

P: Are you a Practical&Technical-Theoretical&Canonical person who can switch to PTC-mood?

The question is, who you are and how you are, if you enter the jukebox of the Canonical Carol.

PTC: Are you a canonical PTC-person who enjoys pushing and popping? Do you like taking things apart, finding new interests in the parts and pushing down further, without loosing the Assembly code? Are you not satisfied with exploring parts B when taking A Apart? Are you trained in the skill to perceive and store the Assembly code for popping back up to the Assembly A?

TT: A TT-personality engages with a deeper-and-deeper Technical-Theoretical interest in pushing down from bodies to cells to bioblasts to molecules to atoms to quarks, without taking note of the canonical assembly code, getting Practically lost.

PT: A PT-person uses the computer in the best Practical&Technical way, but does not take it apart. The PT-personality uses an instrument and software, without curiosity about software code, neither writing software nor assembly instructions. This PT-approach is vitally important to complete an experiment. If you would switch to PTC-mood in the lab, the living cells on the bench would be dead cells before their respiratory rate is measured.

TC: How you proceed as a TC-Irrealist that gets Practically lost, you find out when proceeding to the next Extroductory Canon Voices.

B-major: Taking part in an X-mass party

Subtle dualistic meaning of a term is at the heart of a good joke: the meaning of a term in one context takes us by surprise when pushed into a different box with a paradoxical result, and when we pop up we are laughing, mainly at our own cognitive selves. Recursive laughing is compassionate, terminating laughing is unconcerned. Did you get it: this was no joke? Please, fill in Table B-major 1, particularly if the joke was good, but you did not laugh.

Table B-major 1. Fill in funny Table B-major 1. — Manual to Table B-major 1: If you do not understand English or the English was un-understandable, but the joke was VERY good, then fill in "Good joke? Y" \ "Funny? N". The owner of this Manual declares not to hold any material, philosophical, or psychological responsibility for cases, when the joke was not good ("Good joke? N"), but you did laugh ("Funny? Y").

#	Introduction	Body	Good joke? Y/N	Funny? Y/N	What?
1	A terminating non-laughing double-recursive joke	If it does not make you laugh, don't blame it on the joke. — Why did you not laugh? It was such a good joke!	Y/N	Y/N	The Canonical Carol of jokes
2	BAH! Humbug!	This cannot be completely useless, it can serve as a bad example.	Y/N	Y/N	Humbug
3a	Except at infinity (bottom-up X-mass party)	Parallel lines cannot know what they have in common. They never met.	Y/N	Y/N	Open
3b	On return from infinity (top-down X-mass party)	Parallel lines know what they have in common. That's why they want to never meet again.	Y/N	Y/N	Closed
4	Author: Henny Youngman	When I read about the evils of drinking, I gave up reading.	Y/N	Y/N	Blame it on Henny
5a	Through the looking glass	Looking into a mirror I can't see myself looking.	Y/N	Y/N	Bad quality: eyes or mirror?
5b	I and me like a good mirror	I, for one, like Roman numerals.	Y/N	Y/N	In love with I-self
6	A practical guide to units	Doctor: "I have to tell you quickly that you suffer from a terminal illness and have only ten to live." — Patient: "What do you mean, ten? Ten what? Months? Weeks?!" — Doctor: "Nine."	Y/N	Y/N	By now: "Eight"

In the Practical-Technical world, dualistic terminology is not perceived as a problem. It would be funny, if perceived as a problem. Taking part in an X-mass party is funny only, if you see a problem when there is a problem. If you are Practically focused only on your body mass and BMI, without Theoretical interest in count, volume, and mass, you may skip Voice B-major and continue with B-minor. But don't mix with a biologist, a sport scientist, and a chemist (see Figure B-major 1).

Figure B-major 1. Top-down carolical Canon on parts of the X-mass party, introducing the Mitochondrial physiologist B_M, Sport scientist B_S, and Chemist B_C.

Figure B-major 1a. Highest-level System H(10A[6B]): 6 Buddies B are Assembled per table A, and 10 such Assemblies are together in the Hut. All B enjoy the special air in the hut and maintain healthy distancing with social contact (the meaning of the dashed and full boundary lines is explained in Figure B-major 2d). The Hut has a cosy temperature during the X-mass party, which is felt when entering from the crisp fresh pure air of the mountains. When every Buddy pushed down from the hut, System H(10A[6B]) disappeared and popped out as H(0) (straight to Panel B-major 1d).

Figure B-major 1b. Highest-level System H(1A[6B]): A party-of-six Assemble in an Airway-car on their way to the X-mass party in the Hut. The experiment was to find out, (1) if the body mass of System H(1A[6B]) might crash the Airway-car, and (2) if there was a Ghost in the system. After the party-of-six evacuated the system, the experiment was finished (see Figure B-major 2c for results), and the party-of-six joined the party.

Figure B-major 1c. Highest-level System H(1B): All Buddies with balanced Body mass used a uni-mass balance to measure their Body mass.

H(0) empty.png

Figure B-major 1d. Highest-level System H(0): empty. There is nothing to say about an empty System — it is just a Ghost (see Figure B-major 2a).

An X-mass party pops up in the Highest-level Hut H with a capacity of 60 Bodies B, most of them Biologists and coauthors of BEC 2020.1. One cell biologist just finished a respirometric experiment with cultured cells. Since she wanted to express respiratory rate as O₂ flow per cell I_O2/ce [amol·s^-1·x^-1] and O₂ flux per cell mass I_O2/mce expressed in SI-units [mol·s^-1·kg^-1], she counted the cells for normalization by cell count, and measured the cell mass for normalization by cell mass. As a cell biologist and Mitochondrial phyiologist M, she was trained to consider the cell count N_ce as the number of unit-entities of entity-type X = ce. And for measurement of cell mass, m_X = m_ce, she needed a pretty large number N_ce of cells. That's why pretty M was in the latest group to join the X-mass party, where they were finally escorted to Table H(A₁₀[6B]).

Table B-major 2. No problem on Table H(A₁₀[6B])

#	Speaker	Symbol	Speaked	What
1	Mitochondrial physiologist	M	My name is M. Hello, sorry for being late. Three more Biologists B are with me, and it's kind of you that you other two did not mind waiting a bit.	kind, mind, bit
2	Sport scientist	S	Servus, my name is S. Never mind, we really did not a bit mind this kind of waiting.	kind, mind, bit
3	Chemist	C	C is my name, good evening. Would you mind me pointing out, that this wating time was a bit too long? Now we cannot walk up to system H, but have to Assemble in an Airway-car of the gondola lift; otherwise the High-level Hut would close by the time we pop up there.	kind, mind, bit
4	Sport scientist	S	That's OK. We are all well trained at an average Body mass of 60 kg. So let's make an exception this night and pop up into the Airway-car A as a party-of-six, to pop up at party in the Hut in time.	party
5	Chemist	C	As a Chemist I am not very well trained in physics, but I am sufficiently fit in the basic concepts to clarify, that there is no worry about the type of time we will arrive at the Hut, we will certainly arrive in (1) CEST time, (2) winter time, if we are not further slowed down until summer time, and (3) mechanistic time, if the Airway-car is not that fast to make us worry about relativistic time dilation. But realistically, do we all fit into a single system A?	fit, type
6	Mitochondrial physiologist	M	Easily! I just meausured my Body mass, and we are very similar, such that our body mass is only 360 kg. The Airway-car can take a weight of 400 newton, we are a party-of-six Buddies B NB = 6 x, therefore, our Body mass is 360/6 = 60 kg per Buddy.	Count, Mass
7	Sport scientist	S	Wait - why do biologists talk about simple matters such that they cannot be understood? should be more clear, we talk about Body mass, not weight.	Mass
8	Chemist	C	I remember from physics, that weight is what matters in the Airway-car. This will make a difference when a future X-mass party will take place on Haumea in the Kuiper Belt near Pluto.	Mass
9	Mitochondrial physiologist	M	Sure, we use an approximation under ‘practical’ conditions; wet weight or dry weight should be wet mass or dry mass. There are ongoing discussions, if we should use wet or dry mass for our cells. Anyway, my living cells are dead when I measure their mass.	Living cells
10	Sport scientist	S	Your Body mass is certainly not yet dead when we measure your BMI. We are all fit, since our BMI is about 20 kg∙m-2.	BME
11	Chemist	C	.. and we all fit into system A, let's pop up after all. I cannot wait until our party-of-six joins the party in the Hut.	Extroduction
12	Mitochondrial physiologist	M	Due to my cell mass measurements, we are the last Airway-car to arrive. Look here, this is the last empty table, the Hut is full, and the party goes on as a party of 60 with 10 parties of 6.	party, table
13	Chemist	C	This makes an X-mass party of 3600 kg body mass.	Mass
14	Sport scientist	S	Can you explain the meaning of X?	Entity
15	Chemist	C	Every scientist should know this. In the International System of Units, countable elementary entities get the symbol X.	Entity, SI
16	Sport scientist	S	Does that mean, that you make an X for YOU, when I measure your Body mass?	Entity, Mass
17	Mitochondrial physiologist	M	We should not get personal. Since the majority at our X-mass party are Biologists, we could use the symbol B for our Buddies, such that for you, her, and me we insert generally X = B.	Entity
18	Chemist	C	Wait 60 seconds. IUPAC defines the 'amount of substance concentration' as cB = nB·V-1 [mol·m-3]. X = B then means, that we talk about substance B; entities X can be particles, ions, molecules, ..	Entity, IUPAC
19	Sport scientist	C	No problem. We are used to wait a minute. .. and a Body X = B ..	SI
20	Mitochondrial physiologist	M	.. and all my cells X = ce.	Entity
21	Sport scientist	S	Why do you say 'cells' and not 'cell'?	Count, Entity
22	Mitochondrial biologist	M	Because I am counting the cells, I don't count a cell.	Count
23	Sport scientist	S	I don't count Bodies, I just put you on my mass balance.	Mass
24	Mitochondrial biologist	M	That is your problem, not the problem of your balance. Your mass balance measures, it does not count and does not know how many bodies you have sampled for your mass determination. You take a count NB = 1xB, and then you put your sample on the balance, when you measure my Body mass. Again, don't take it personal, since I am just single; but I don't work with single cells. Your B is a single Body, my ce are many many cells. In my cell mass mce of a few µg there are many cells.	Count, single
25	Sport scientist	S	But your Body mass is 60 kg. So your cell mass cannot be just a few 0.000001 kg!	my Mass
26	Chemist	C	M does not talk about her cells in her Body M, as much as you don't talk about your mass balance, which is the balance of all mass going into your system S (input = food and drink and O2) and out of your system S (output = .. let's not talk about it on the table).	my mass balance
27	Sport scientist	S	At least we can mention CO2, and list all components of the mass balance in a Table.	table
28	Mitochondrial biologist	M	I don't want to be in your table. Of course, I had in mind my cells on my bench in my lab.	table
29	Sport scientist	S	Now you put everything on the table. So you push your cells out of your body M and pop them up to your mass balance on your bench in your lab?	table
30	Mitochondrial biologist	M	You don't understand! With my cells in my mind, I am not talking about ce in system B (or in system M for my own Body), nor about ce from system B (or from system M for my own Body), but in my mind is a specific neuronal cell type X = ce, cultured in my lab.	System, Entity
31	Chemist	C	You better get your brain cells out of your mind. When I talk about my chemicals, it is those moles of substance B that are not in me nor taken from me but prepared by me.	Mole
32	Sport scientist	S	When I measure your Body mass, then I am looking at all those kilograms that are in your Body.	Mass per Entity
33	Mitochondrial biologist	M	Can my body mass be in my Body? Is'nt my body mass my Body? Can my body mass be in my body mass? I thought we agreed that in 'my cell mass' and in 'my Body mass' the recursive "my" presents the problem.	my my
34	Chemist	C	That would be too simple. The mass mB of my sample of substance B is not the Body mass of a single Bit of B.	Mass of Entities
35	Mitochondrial biologist	M	.. as my cell mass mce is not the mass of a single cell.	Mass of Entities
36	Sport scientist	S	Everybody knows that your Body mass is the mass of your own Body. Why should I think of mB as the mass of many Bodies?	Mass per Entity
37	Mitochondrial biologist	M	We had this already on the way up, when we popped up into A. Then mB was the mass of our Bodies in the Airway-car, mB = 360 kg.	Mass of Entities
38	Chemist	C	Back to the X-mass party, now all A popped up in system H. Now mB = 3600 kg in the Hut.	System
39	Sport scientist	S	Now I want to push back to me: If I measure my Body mass mB = 60 kg myself, then my Body mass is in system B.	Entity, System
40	Chemist	C	No more pushing an popping: you cannot push entity B into system B, nor pop B into B.	Entity, System
41	Mitochondrial biologist	M	This sounds like my Body mass does not exist, since it does not fit anywhere — neither can it be pushed down nor popped up.	Entity
42	Sport scientist	S	This would be bad news for your BMI — there cannot be an Index without Body Mass. But where is the problem?	BME
43	Mitochondrial biologist	M	There is no problem, but on our table we are talking all the time about it's solution. Look at the other Table (BEC 2020.1, Table 4)	BEC 2020.1
44	Sport scientist	S	??? .. We need more Beers for the Extroductory Canon Voice B-minor.	Extroduction

The Mitochondrial physiologist, Sport scientist and Chemist remember that they had joined these parties already six times, and always presented the X-mass Carol. The Mitochondrial physiologist proclaimed, that she brought 60 hard copies of BEC 2020.1, so for the first time they could all make a joint presentation in harmony. Before chanting the Carolical Canon, however, M explains to System H, that an Extroduction is required to be able to fully enjoy the X-mass Carol, which is and introduction to the Canonical Carol, which is a push-down from BEC 2020.1, which is recorded here, if you want to double-pop up from the Extroduction: »BEC 2020.1«.

If you pushed down again or stayed in System H, you will be interested to know, what they were chanting. After some more Beer, they remembered the terms, symbols, and units in their Canonical Carol in the spirit of BEC 2020.1, and to the amazement of the audience in System H, which includes our party-of-six A₁₀(6B), they carol ... To not even enter an endless loop at this node of the X-mass story, here is a pseudo-pop up to the X-mass Carol, summarized in Table B-major 3 and Figure B-major 2:

Table B-major 3. No problem on Table H(A₁₀[6B])

Term	Symbol	Units	Definition	What
mass of entity-type X	mce	[kg]	The mass of all cultured cells of entity-type ce in the sample that was used to measure the cell mass, or in a sample popped up into the High-resolution respirometer.	Entity
mass of entity-type X	mB	[kg]	The mass of all biologists of entity-type B in the party of NB = 6, who entered the Airway-car A.	mB = 360 kg
mass per unit-entity of entity-type X	MUB	[kg·x-1]	The mass of a unit-entity UB of entity-type B.	mUB = 60 kg·x-1

Figure B-major 2. Bottom-up canonical Carol on parts of the X-mass party

H(0) empty.png

Figure B-major 2a. Highest-level System H(0): empty. The empty System H(0) has unique features. Depending on its bottom-up or top-down evolution, it has not yet gained or already lost it's internal identity: there are no countable objects, but also no H in the empty system. It does not contain any count. It contains emptiness and darkness and silence. It is full of vacuum (if the gas phase is taken into account upon evacuation of the Hut or the Airway-car). But it would be wrong to say that it does not have any mass, since the mass of a system of vacuum is m_H(0) = 0 kg. H(0) is the Ghost G of System H(N_A[N_B]) and System H(N_B), with N_A > 0 x and N_B > 0 x, otherwise G would be the Ghost of a ghost. The Ghost has no internal identity, but it has external extent. Without any count, with mass of zero kg, the empty System H(0) still has the volume of the ghost-experimental System: It has no sound, no light, no matter, no count, it has space — and this does not matter. When you empty the experimental chamber, you put a Ghost into your instrumental chamber. When you disassemble your instrument and remove the instrumental chamber, you are loosing the Ghost. The empty chamber on the shelf or in the recycling bin has no Ghost. But keeping in mind the X-mass party on the top in the Hut, it is now clear that there is a friendly Ghost in the empty Hut, which gets lost when the hut collapses or burns down.

Figure B-major 2b. Highest-level System H(1B): The Buddies B got prepared for the X-mass party: a party-of-six indicated as Assembly A₁₀(6B) in an ensemble of 10 Assemblies of type A(6B) popped up to a mass balance, to qualify for an experiment planned with experimental system Highest-level System H(1A[6B]). But they did not pop up to a party-of-six balance. Instead, they used their uni-mass balances. A uni-mass balance is a multi-detector/sensor instrument. When a count N_B = 1x pops up to the Highest-level System H(1B), the uni-mass balance (1) has a detector which converts a signal indicating that it has become the fully populated System H(1B) into a count N_B = 1 x and uses this information to block any uncontrolled overpopulation to a System H(N_B > 1 x) —, and (2) it has a sensor for measuring the difference of the mass of the full system minus the mass of the empty system. Then you have a uni-mass multi-detector/sensor balance. This System H(1B) contains the air around entity-type B, and since entity-type B is not a gaseous object, this mass of air is ignored in practical evaluations of the mass of System H(1B). Therefore, when object B_M popped up on the uni-mass multi-detector/sensor balance, the mass of System H(1B) was 60 kg. When object B_M pushed down from the balance, she did not cofuse the previously measured mass of System H(1B) — which now is an empty Ghost at m_H(0) = 0 kg (see Panel 2a) — with her non-ghostly living Body mass, M_UB = m_H(1B)·N_B = 60 kg·x^-1.

Figure B-major 2c. Highest-level System H(1A[6B]): A party-of-six N_B = 6xB are an Assembly in an Airway-car on their way to the X-mass party in the Hut. While the party-of-six formed a group of loosely distributed particles B in front of the gondola lift, they popped up into System H(1A[6B]) when entering the Air-conditioned Airway-Car. System H(1A(6B)), therefore, is not Assembly A, but contains the single group A, N_A = 1 x, in the Airspace of the Airway-car. Nor is the Airway-car identical with System H(1A(6B)), since H(1A(6B)) is the contents without the glass of the windows and the steel of the walls of the container, whereas the Airway-car is the container enclosing System H. The Airway-car is the instrumental system, System H(1A[6B]) is the experimental System. The experiment was to find out, (1) if any Body entering H(0) can find the Ghost, and (2) if the body mass of System H (m_B = 360 kg) with a full group A (N_B = 6 x) did not exceed the crash-hold of the instrumental system Airway-car. The mass of the instrumental system was much higher than the mass of the experimental System H(1A[6B]), even after A pushes down on exit (near the hut) and System H(1A[6B]) is evacuated to H(0). Once the party-of-six pops up to into the Hut, the experiment H(1A[6B]) is finished, System H(1A[6B]) pops out of existence, whereas the Airway-car remains intact, and the Assembly A₁₀(6B) or party-of-six joins and enjoys the party. Summary of the experiment: (1) The First Law of Ghost-Thermodynamics says, that no Body can ever find the Ghost in the empty system when popping up into the system. (2) The working hypothesis was not stated clearly. (2a) The result may have been negative, since their body mass did not crash the Airway-car. (2b) The result was positive, since their body mass was maintained in a living thermodynamic state as an inclusion criterion for the X-mass party.

Figure B-major 2d. Highest-level System H(10A[6B]): 6 Buddies B are Assembled per table A, and 10 such Assemblies are together in the Hut. An Assembly in the Hut is a permeable compartment containing assembled countable entities B as indicated by the dashed boundary lines, bound together in their seats around a table, sharing the same High-quality gaseous medium in system H with the other permeable Assemblies, since air is practically well mixed across Assemblies A in the Hut, whereas the Bodies B do not practically share their internal milieux enclosed by full circles (on the alp there is no sin). A are, but B are not in thermal equilibrium with H. Similarly, the Hut is well heated during the X-mass party, such that the internal High-quality gaseous medium of the Hut is not in thermal equilibrium with the outside world. In the terminology of canonical ensembles, this external part or compartment is called the 'heat bath', which in case of System H is neither hot nor a bath, but it is the crisp fresh pure air of the mountains. System H does not contain a pure sample, but a mixture of A containing structurally positioned elementary-units B. Before A₁₀(6B) popped up, System H(10₉A[B6]) was not full. When every Buddy pushed down from the hut, System H(10A[6B]) disappeared and popped out as H(0) (straight back to the Ghost story in Panel B-major 2a).

O: A Canonical O2k-Manual for high-resolution respirometry: why the living cells died and dyed

Q1: The question is, who you are and how you are, if you enter the Canoncal O2k-Manual in the jukebox of the Canonical Carol.

Q1(Q2): Is the DatLab software of the high-resolution respirometer HRR (which is installed on my PC [that is connected to my O2k]) part of the O2k?

In the O2k-Catalogue, DatLab is listed as part of the O2k, which was shipped in a Hardware box H, that contained the hardware O2k H(O2k), and a separate USB-flashDrive, which is the Software box D. After assembly of the O2k, H is unfortunately empty, since there is no instruction on how to copy the O2k before taking it out of the box — this would be a most economical approach to the Power-O2k. This is different with the Software box D, since there is an instruction to copy DatLab rather than taking it out of the box D, such that DatLab stays inside the box D containing DatLab, represented as D(atLab).

Before opening box H, it is instructive to clarify the canonical representation of H, to address question Q1(Q2), if DatLab is part of the O2k.

1. YES, then H(O2k[D{atlab}])
2. NO, then H(O2k, D[atlab])
3. Before deciding too quickly, consider the important higher-level question in this nested network: Is H part of the O2k? — O2k(H[O2k{D<atLab>}])
4. Postpone the decision and think of your living-dying cells on the bench - now we are a bit practical even in Theoretical&Canonical TC-mode.

Q1(Q2[Q3]): Is the instruction 'Add cells' in the O2k-Manual F(Add cells) part of the O2k-Hardware or Software, and where is the F.. manual?

We should add the cells into the O2k for HRR measurements. If I proceed with the perception that DatLab is part of the O2k-system, then the part of the O2k-Manual that instructs me to add the cells into the O2k should explicitly (canonically) define, into which part the cells are to be addded, else a TC-user may try to add the cells into the USB-flashDrive D(atlab). It goes without saying that the proper part where to add the sample are the O2k-Chambers 2K (for the German 'Kammer' for chamber, and there are N_K = 2 x K chambers used in the O2k). The O2k-Manual says, that the O2k should be taken out of the Hardware-box H, DatLab should be copied from the Software-box D(atlab) to be installed on my PC, and — BINGO! Here is the answer to Q1(Q2) in Voice O: DatLab is not part of my O2k, the O2k-Catalogue should get this right for all TC-users (neither PT-Realists nor TT-Nerds would care).

The O2k-Manual shows in a video, how two O2k-Chambers are taken out of the Assembly box A(3K) → A(1K) + 2K, pushing down from system A to entity K, leaving one spare O2k-Chamber in box A(1K). This is followed by popping up 2K to the O2k-System S. In my TC-mode there is a Theoretical voice that says: Think of normalization, count the cells first. The Technical side transfers a subsample of cells to the cell counter. And here comes the Canonical again.

Do the instructions of the cell counter include the unit 'counting-unit' [x] in their system of units? Does the cell counter manual introduce properly the quantity 'count' with symbol N_X, meaning 'number of unit-entities of unit-type X, expressed in the unit 'counting-unit' with symbol [x], where in the cell counter X = ce? If I do not re-write the cell counter manual at this stage, since the living cells are dying on the bench, I should nevertheless update the 'Add cells' instructions in the O2k-Manual.

Where is the O2k-Manual F (pronounced as 'the F.. manual')? The instruction says: H(O2k, D[adtlab, F{DatLab, Assembly, Operation, Disassembly}]) ↓ push down to find the USB-FlashDrive D(adtlab, F[DatLab, Assembly, Operation, Disassembly]) in box H and copy the Manual F(Assembly, Operation, DatLab). But since the living cells are dying on the bench, there is no time to read the F.. manual. Just look for 'Add cells'. ↓ push down to find 'Add cells'. There are several options for pushing, with failures and problems:

1. Manual F(DatLab, Assembly, Operation[Add cells], Disassembly) — failed, take an alternative thread, mining and grinding on the same level without pushing:
2. Manual F(DatLab[Add cells], Assembly, Operation) — failed, give up grinding and pop up
3. D(adtlab, F[DatLab, Assembly, Operation, Disassembly]) — ?? — switch from system D to system PC, where you have installed DatLab and DatLab is running
4. PC(DatLab[DL-Protocols{F<How to Find instructions in F, Add cells>}]) — Found !!

The great success to find the instruction 'Add cells' is a great testimony to the fact, that the O2k-Manual is the most simple, logical, practical, easy-to-use, userfriendly instrumental manual of type F in the world. There exists no simpler, logical, easy-to-use structure of F:

1. HHH: push down taking all you need out of the box or copy the software.
2. O2k↔PC(DatLab): pop up (set up) the instrumental system with your PC and DatLab connected to the O2k.
3. 2K: pop up (insert) the instrumental chambers into the O2k
4. H: get started with High-resolution respirometry: switch on the O2k, connect DatLab, select a DL-Protocol for 'cells', define the experimental temperature of your experimental system, add mitochondrial respiration medium.
5. DatLab(DL-Protocols[F{How to Find instructions in F, Add cells}]): In DatLab, the selected DL-Protocols designed for 'cells' as a sample type, include within the DL-Protocol the F-Manual, which provides instructions how to Find help, and where you find the instruction on 'Add cells'.

There is no way to make this structure of the nested system simpler, more logical, easy-to-use. So how do you find this structure? You may find it to be useful, but in the first place you do not find the structure at all. When trying to Find the instruction 'Add cells', we are not trying to find information on how to Find, but without any choice we have to use the structure of F to Find 'Add cells'. We cannot best use a structure of a nested system, if we are not pushing down into the instructions to find instructions, without forgetting to pop up back to the current task. If you have time to play pusing and popping, even complex structures will precipitate. This is how learn using a new software when we like to play with it, and the more PTC- one adds to a PT-approach, the more sophisticated and error-free applications are possible. Any sophistication of TC-mode does not help in using or designing a nested structure, if the practical side is missing. Therefore, diversity of operation- and cognition-modes is the key to innovation of design and success of application, based on gender balance, cultural diversity, Extroductory Voices in the team — if only they can communicate.

Of course, there is of simplifying the structure of O2k-Manual F and making it even more logical and canonical. Oroboros, please stack all Operation instructions into DatLab, such that failures and problems 1-3 above will not happen, even without thinking about the structure of F. Change from:

1. D(adtlab[DL-Protocols{F<How to Find instructions in F, Add cells> F[DatLab, Assembly, Operation, Disassembly]), to let all external F disappear altogether, devoured by DatLab itself, to obtain the linear thread,
2. D(adtlab[DL-Protocols{F<How to Find instructions in F, Assembly, Operations<How to operate DatLab, How to operate the O2k»Add cells«>, Disassembly}])

No more frustration in Finding everything on the O2k in DatLab and everything on DatLab in DatLab. With its self-referencing, retrograde structure this linear thread gets fuzzy on recursive nodes, and canonical perfection can never be achieved in a perfectly linear structure: If all instructions on the O2k and DatLab are nested in DatLab, where do you find the instruction how to find DatLab? The instruction on how to open the box must not be popped into the box. F on F nested in the box implies infinite regress without bottoming out, and all you need is falling in love with the Extroduction that contemplates about the system in the box and out of the box.

With too much obsession by the Extroduction, the living cells have died on the bench and their nuclei dyed in the cell counter. At this stage, exclusively continued TC-mode of the Unrealist provides escape. I have a living cell preparation on the bench, but all are dying, without application of any mild detergent to permeabilize the plasma membranes enabling the dying of all nuclei in a cell viability test. Being aware of this theoretical question, I prepared a parallel subsample as a negative control for the cell viability test: experimental sample 'ce' = living cells; negative control sample 'pce' = experimentally permeabilized cells using digitonin. In the TC-approach all living cells were dying, such that sample 'ce' is not intact any more when it should be popped into the 2K of the O2k. The cell count N_ce of the experimental sample is identical to the cell count N_pce of the negative control. The cell viability index IV_ce = N_vce·N_ce^-1 of the experimental group and the cell viability index IV_pce = N_vce·N_pce^-1 of the negative control group are identical, both are exactly zero, since subscript 'vce' indicates 'viable cells'. This is fine for the pce-control cells. But an appropriate term is required for the ce-experimental cells. These living cells ce are not intact any longer — they are certainly not intact cells. But these living cells are not viable cells at a viability = 0. But this experimental sample ce should have a viability >0.95 in a more Practical and less Canonical approach. This is where Mitochondrial physiology bottoms out BEC 2020.1.

References

1. Ioannidis JP, Greenland S, Hlatky MA, Khoury MJ, Macleod MR, Moher D, Schulz KF, Tibshirani R (2014) Increasing value and reducing waste in research design, conduct, and analysis. Lancet 383:166-75.
2. Boltzmann L (1877) Über die Beziehung zwischen dem zweiten Hauptsatze der mechanischen Wärmetheorie und der Wahrscheinlichkeitsrechnung, respektive den Sätzen über das Wäremegleichgewicht. Sitzb d Kaiserlichen Akademie der Wissenschaften mathematich-naturwissen Cl LXXVI, Abt II:373-435.
3. Gallavotti G (2014) Nonequilibrium and irreversibility. Springer, Heidelberg, New York, London.
4. Bureau International des Poids et Mesures (2019) The International System of Units (SI). 9th edition:117-216 ISBN 978-92-822-2272-0.
5. Cohen ER, Cvitas T, Frey JG, Holmström B, Kuchitsu K, Marquardt R, Mills I, Pavese F, Quack M, Stohner J, Strauss HL, Takami M, Thor HL (2008) Quantities, Units and Symbols in Physical Chemistry. IUPAC Green Book 3rd Edition, 2nd Printing, IUPAC & RSC Publishing, Cambridge.
6. Hofstadter DR (1979) Gödel, Escher, Bach: An eternal golden braid. A metaphorical fugue on minds and machines in the spirit of Lewis Carroll. Harvester Press:499 pp.
7. Gnaiger Erich et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. doi:10.26124/bec:2020-0001.v1.

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