Difference between revisions of "Vinnakota 2016a Biophys J"
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{{Publication | {{Publication | ||
|title=Vinnakota KC, Bazil JN, Van den Bergh F, Wiseman RW, Beard DA (2016) Feedback regulation and time hierarchy of oxidative phosphorylation in cardiac mitochondria. Biophys J 110:972-80. Β | |title=Vinnakota KC, Bazil JN, Van den Bergh F, Wiseman RW, Beard DA (2016) Feedback regulation and time hierarchy of oxidative phosphorylation in cardiac mitochondria. Biophys J 110:972-80. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/26910434 PMID: 26910434 Open Access] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/26910434 PMID: 26910434 Open Access] | ||
|authors=Vinnakota KC, Bazil JN, Van den Bergh F, Wiseman RW, Beard DA | |authors=Vinnakota KC, Bazil JN, Van den Bergh F, Wiseman RW, Beard DA | ||
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Copyright Β© 2016 Biophysical Society. Published by Elsevier Inc. All rights reserved. | Copyright Β© 2016 Biophysical Society. Published by Elsevier Inc. All rights reserved. | ||
|keywords=Decylubiquinone | |||
|mipnetlab=US MI Ann Arbor Beard DA, US MI East Lansing Bazil JN | |||
}} | }} | ||
{{Labeling | {{Labeling | ||
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|tissues=Heart | |tissues=Heart | ||
|preparations=Isolated mitochondria | |preparations=Isolated mitochondria | ||
|topics=ADP, ATP, Flux control, Phosphate | |||
|topics=ATP, | |||
|couplingstates=LEAK, OXPHOS | |couplingstates=LEAK, OXPHOS | ||
|instruments=Oxygraph-2k | |pathways=N | ||
|additional= | |instruments=Oxygraph-2k, TPP | ||
|additional=2016-03 | |||
}} | }} |
Latest revision as of 16:29, 8 April 2020
Vinnakota KC, Bazil JN, Van den Bergh F, Wiseman RW, Beard DA (2016) Feedback regulation and time hierarchy of oxidative phosphorylation in cardiac mitochondria. Biophys J 110:972-80. |
Vinnakota KC, Bazil JN, Van den Bergh F, Wiseman RW, Beard DA (2016) Biophys J
Abstract: To determine how oxidative ATP synthesis is regulated in the heart, the responses of cardiac mitochondria oxidizing pyruvate to alterations in [ATP], [ADP], and inorganic phosphate ([Pi]) were characterized over a range of steady-state levels of extramitochondrial [ATP], [ADP], and [Pi]. Evolution of the steady states of the measured variables with the flux of respiration shows that: (1) a higher phosphorylation potential is achieved by mitochondria at higher [Pi] for a given flux of respiration; (2) the time hierarchy of oxidative phosphorylation is given by phosphorylation subsystem, electron transport chain, and substrate dehydrogenation subsystems listed in increasing order of their response times; (3) the matrix ATP hydrolysis mass action ratio [ADP] Γ [Pi]/[ATP] provides feedback to the substrate dehydrogenation flux over the entire range of respiratory flux examined in this study; and finally, (4) contrary to previous models of regulation of oxidative phosphorylation, [Pi] does not modulate the activity of complex III.
Copyright Β© 2016 Biophysical Society. Published by Elsevier Inc. All rights reserved. β’ Keywords: Decylubiquinone
β’ O2k-Network Lab: US MI Ann Arbor Beard DA, US MI East Lansing Bazil JN
Labels: MiParea: Respiration
Organism: Rat
Tissue;cell: Heart
Preparation: Isolated mitochondria
Regulation: ADP, ATP, Flux control, Phosphate Coupling state: LEAK, OXPHOS Pathway: N HRR: Oxygraph-2k, TPP
2016-03