Difference between revisions of "Varga 2017 Inflammopharmacology"
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{{Publication | {{Publication | ||
|title=Varga G, Ugocsai M, Hartmann P, Lajkó N, Molnár R, Szűcs S, Jász DK, Érces D, Ghyczy M, Tóth G, Boros M (2017) Acetylsalicylic acid-tris-hydroxymethyl-aminomethane reduces colon mucosal damage without causing gastric side effects in a rat model of colitis. Inflammopharmacology | |title=Varga G, Ugocsai M, Hartmann P, Lajkó N, Molnár R, Szűcs S, Jász DK, Érces D, Ghyczy M, Tóth G, Boros M (2017) Acetylsalicylic acid-tris-hydroxymethyl-aminomethane reduces colon mucosal damage without causing gastric side effects in a rat model of colitis. Inflammopharmacology 26:261-71. | ||
|info=[https://www.ncbi.nlm.nih.gov/pubmed/28451776 PMID: 28451776] | |info=[https://www.ncbi.nlm.nih.gov/pubmed/28451776 PMID: 28451776] | ||
|authors=Varga G, Ugocsai M, Hartmann P, Lajko N, Molnar R, Szucs S, Jasz DK, Erces D, Ghyczy M, Toth G, Boros M | |authors=Varga G, Ugocsai M, Hartmann P, Lajko N, Molnar R, Szucs S, Jasz DK, Erces D, Ghyczy M, Toth G, Boros M | ||
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|tissues=Liver | |tissues=Liver | ||
|preparations=Isolated mitochondria | |preparations=Isolated mitochondria | ||
|couplingstates=OXPHOS | |couplingstates=LEAK, OXPHOS | ||
|pathways=N, S, CIV, NS | |pathways=N, S, CIV, NS, ROX | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional= | |additional=2017-08, | ||
}} | }} |
Latest revision as of 08:28, 28 March 2018
Varga G, Ugocsai M, Hartmann P, Lajkó N, Molnár R, Szűcs S, Jász DK, Érces D, Ghyczy M, Tóth G, Boros M (2017) Acetylsalicylic acid-tris-hydroxymethyl-aminomethane reduces colon mucosal damage without causing gastric side effects in a rat model of colitis. Inflammopharmacology 26:261-71. |
Varga G, Ugocsai M, Hartmann P, Lajko N, Molnar R, Szucs S, Jasz DK, Erces D, Ghyczy M, Toth G, Boros M (2017) Inflammopharmacology
Abstract: We have developed a novel compound from acetylsalicylic acid (ASA) and 2-amino-2-(hydroxymethyl)-1,3-propanediol (Tris) precursors with ASA-like anti-inflammatory efficacy and reduced the mucosa-damaging side-effects. Our aim was to examine local and remote consequences of ASA-Tris administration in 2-,4-,6-trinitrobenzene-sulfonic acid (TNBS)-induced colitis as compared to ASA or mesalamine (5-aminosalicylate) treatment.
Sprague-Dawley rats were randomized to five groups (n = 6, each), and TNBS enemas were performed. Group 1 was the negative control; group 2 was the untreated colitis group. 12 hour after colitis induction repeated doses of ASA, ASA-Tris (both 0.55 mmol/kg) and mesalamine (0.77 mmol/kg) were given 3 times daily for 3 days to groups 3-5. On day 3 of colitis, the in vivo histology of the colon and stomach was investigated. Tissue xanthine-oxidoreductase, myeloperoxidase, nitrite/nitrate changes, and circulating TNF-alpha levels were measured. In addition, liver mitochondria were examined with high-resolution respirometry to analyze alterations in the electron transport chain.
TNBS enema significantly elevated inflammatory enzyme activities, NO production, TNF-alpha concentration, and induced morphological damage in the colon. ASA-treatment reduced the inflammatory marker levels and mucosal injury in the colon, but gastric tissue damage was present. ASA-Tris- and mesalamine-treatments significantly reduced the cytokine levels, inflammatory enzyme activities, and colonic mucosal damage without inducing gastric injury. Also, ASA significantly reduced the Complex IV-linked respiration of liver mitochondria, which was not observed after ASA-Tris-treatment.
As compared to ASA, ASA-Tris conjugation provides significant protection against the colonic injury and cytokine-mediated progression of inflammatory events in experimental colitis without influencing the gastric epithelial structure. • Keywords: Acetylsalicylic-acid; Gastritis; Mitochondria; Non-steroidal anti-inflammatory drugs; TNBS colitis • Bioblast editor: Kandolf G • O2k-Network Lab: HU Szeged Boros M
Labels: MiParea: Respiration, Pharmacology;toxicology
Pathology: Infectious, Other
Organism: Rat Tissue;cell: Liver Preparation: Isolated mitochondria
Coupling state: LEAK, OXPHOS
Pathway: N, S, CIV, NS, ROX
HRR: Oxygraph-2k
2017-08