Uncoupled respiration: Difference between revisions
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The uncoupled part of respiration in state ''P'' is larger than LEAK respiration evaluated in state ''L'' after inhibition of [[ATP synthase]] or [[adenine nucleotide translocase]]. This is due to the increase of mt-membrane potential in state ''L'' versus ''P'', causing a corresponding increase of the proton leak driven by the higher proton motive force. As an approximation, however, the difference ''E''-''L'' yields an estimate of the physiological scope of uncoupling, or the pathological scope of dyscoupling. | |||
Revision as of 21:47, 18 September 2010
- high-resolution terminology - matching measurements at high-resolution
Uncoupled respiration
Description
The uncoupled part of respiration in state P pumps protons to compensate for intrinsic uncoupling, which is a property of:
(a) the inner mt-membrane (proton leak),
(b) the proton pumps (proton slip; decoupling), and
(c) is regulated by molecular uncouplers (uncoupling protein, UCP1).
Uncoupled and dyscoupled respiration are summarized as LEAK respiration. In contrast, non-coupled respiration is induced experimentally for evaluation of ETS capacity.
Reference: MiPNet12.15, MiPNet10.04
Labels:
Enzyme: Uncoupler Protein"Uncoupler Protein" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property. Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Coupling; Membrane Potential"Coupling; Membrane Potential" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
The uncoupled part of respiration in state P is larger than LEAK respiration evaluated in state L after inhibition of ATP synthase or adenine nucleotide translocase. This is due to the increase of mt-membrane potential in state L versus P, causing a corresponding increase of the proton leak driven by the higher proton motive force. As an approximation, however, the difference E-L yields an estimate of the physiological scope of uncoupling, or the pathological scope of dyscoupling.
