Difference between revisions of "Tyrrell 2016 Redox Biol"
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{{Publication | {{Publication | ||
|title=Tyrrell DJ, Bharadwaj MS, Jorgensen MJ, Register TC, Molina AJ (2016) Blood cell respirometry is associated with skeletal and cardiac muscle bioenergetics: Implications for a minimally invasive biomarker of mitochondrial health. Redox Biol 10:65-77. Β | |title=Tyrrell DJ, Bharadwaj MS, Jorgensen MJ, Register TC, Molina AJ (2016) Blood cell respirometry is associated with skeletal and cardiac muscle bioenergetics: Implications for a minimally invasive biomarker of mitochondrial health. Redox Biol 10:65-77. | ||
|info=[https://www.ncbi.nlm.nih.gov/pubmed/27693859 PMID: 27693859 Open Access] | |info=[https://www.ncbi.nlm.nih.gov/pubmed/27693859 PMID: 27693859 Open Access] | ||
|authors=Tyrrell DJ, Bharadwaj MS, Jorgensen MJ, Register TC, Molina AJ | |authors=Tyrrell DJ, Bharadwaj MS, Jorgensen MJ, Register TC, Molina AJ | ||
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Copyright Β© 2016 The Authors. Published by Elsevier B.V. All rights reserved. | Copyright Β© 2016 The Authors. Published by Elsevier B.V. All rights reserved. | ||
|keywords=Bioenergetics, Blood cells, Cellular respiration, Mitochondria, Muscle, PBMC | |keywords=Bioenergetics, Blood cells, Cellular respiration, Mitochondria, Muscle, PBMC, Buffer z, Blebbistatin | ||
|mipnetlab=US NC Winston-Salem Molina AJA | |mipnetlab=US NC Winston-Salem Molina AJA | ||
}} | }} | ||
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|tissues=Skeletal muscle | |tissues=Skeletal muscle | ||
|preparations=Permeabilized tissue | |preparations=Permeabilized tissue | ||
|couplingstates=OXPHOS, ETS | |couplingstates=LEAK, OXPHOS, ETS | ||
|pathways=N, NS, ROX | |pathways=N, NS, ROX | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional= | |additional=2016-11, | ||
}} | }} |
Revision as of 13:39, 2 August 2017
Tyrrell DJ, Bharadwaj MS, Jorgensen MJ, Register TC, Molina AJ (2016) Blood cell respirometry is associated with skeletal and cardiac muscle bioenergetics: Implications for a minimally invasive biomarker of mitochondrial health. Redox Biol 10:65-77. |
Tyrrell DJ, Bharadwaj MS, Jorgensen MJ, Register TC, Molina AJ (2016) Redox Biol
Abstract: Blood based bioenergetic profiling strategies are emerging as potential reporters of systemic mitochondrial function; however, the extent to which these measures reflect the bioenergetic capacity of other tissues is not known. The premise of this work is that highly metabolically active tissues, such as skeletal and cardiac muscle, are susceptible to differences in systemic bioenergetic capacity. Therefore, we tested whether the respiratory capacity of blood cells, monocytes and platelets, are related to contemporaneous respirometric assessments of skeletal and cardiac muscle mitochondria. 18 female vervet/African green monkeys (Chlorocebus aethiops sabaeus) of varying age and metabolic status were examined for this study. Monocyte and platelet maximal capacity correlated with maximal oxidative phosphorylation capacity of permeabilized skeletal muscle (R=0.75, 95% confidence interval [CI]: 0.38-0.97; R=0.51, 95%CI: 0.05-0.81; respectively), isolated skeletal muscle mitochondrial respiratory control ratio (RCR; R=0.70, 95%CI: 0.35-0.89; R=0.64, 95%CI: 0.23-0.98; respectively), and isolated cardiac muscle mitochondrial RCR (R=0.55, 95%CI: 0.22-0.86; R=0.58, 95%CI: 0.22-0.85; respectively). These results suggest that blood based bioenergetic profiling may be used to report on the bioenergetic capacity of muscle tissues. Blood cell respirometry represents an attractive alternative to tissue based assessments of mitochondrial function in human studies based on ease of access and the minimal participant burden required by these measures.
Copyright Β© 2016 The Authors. Published by Elsevier B.V. All rights reserved. β’ Keywords: Bioenergetics, Blood cells, Cellular respiration, Mitochondria, Muscle, PBMC, Buffer z, Blebbistatin
β’ O2k-Network Lab: US NC Winston-Salem Molina AJA
Labels: MiParea: Respiration
Organism: Other mammals
Tissue;cell: Skeletal muscle
Preparation: Permeabilized tissue
Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
Pathway: N, NS, ROX
HRR: Oxygraph-2k
2016-11