Difference between revisions of "Template:SUIT text D029"

Revision as of 16:02, 19 July 2019

SUIT-006 O2 ce-pce D029 is a coupling control protocol for permeabilized cells. Living cells are added in the chamber, allowing to analyse ROUTINE respiration. The cells are further permeabilized inside the O2k chamber, and different coupling control states are assessed (L(n) - P - L(Omy) - E) at N(PM) pathway control state. The use of oligomycin is optional, however, it provides important information when residual and endogenous adenylates are present (which may happen if ATPases are active in the sample). This situation may lead to overestimated LEAK respiration measured in the absence of adenylates - L(n). Therefore, oligomycin can be used to verify whether this occurs and obtain the LEAK state appropriately.

Revision as of 16:01, 19 July 2019 (view source) Antunes Diana (talk \| contribs) (Created page with "SUIT-006 O2 ce-pce D029 is a coupling control protocol for permeabilized cells. Intact cells are added in the chamber, allowing to analyse ROUTINE respiration. The cel...")		Revision as of 16:02, 19 July 2019 (view source) Antunes Diana (talk \| contribs) Newer edit →
Line 1:		Line 1:
	SUIT-006 O2 ce-pce D029 is a [[coupling control protocol]] for permeabilized cells. ~~Intact~~ cells are added in the chamber, allowing to analyse [[ROUTINE]] respiration. The cells are further permeabilized inside the O2k chamber, and different coupling control states are assessed ([[LEAK-respiration\|''L''(n)]] - [[Oxidative phosphorylation\| ''P'']] - [[LEAK-respiration\|''L''(Omy)]] - [[ET-capacity\| ''E'']]) at [[PM pathway control state\|N(PM) pathway control state]]. The use of [[oligomycin]] is optional, however, it provides important information when residual and endogenous adenylates are present (which may happen if ATPases are active in the sample). This situation may lead to overestimated [[LEAK]] respiration measured in the absence of adenylates - [[LEAK state without adenylates\|L(n)]]. Therefore, [[oligomycin]] can be used to verify whether this occurs and obtain the [[LEAK]] state appropriately.		SUIT-006 O2 ce-pce D029 is a [[coupling control protocol]] for permeabilized cells. Living cells are added in the chamber, allowing to analyse [[ROUTINE]] respiration. The cells are further permeabilized inside the O2k chamber, and different coupling control states are assessed ([[LEAK-respiration\|''L''(n)]] - [[Oxidative phosphorylation\| ''P'']] - [[LEAK-respiration\|''L''(Omy)]] - [[ET-capacity\| ''E'']]) at [[PM pathway control state\|N(PM) pathway control state]]. The use of [[oligomycin]] is optional, however, it provides important information when residual and endogenous adenylates are present (which may happen if ATPases are active in the sample). This situation may lead to overestimated [[LEAK]] respiration measured in the absence of adenylates - [[LEAK state without adenylates\|L(n)]]. Therefore, [[oligomycin]] can be used to verify whether this occurs and obtain the [[LEAK]] state appropriately.