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Template:SUIT text D028

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SUIT-003 O2 ce D028 is a coupling-control protocol for living cells. Different coupling control states are assessed (ROUTINE - L(Omy) - E) at ce-substrate state. The use of oligomycin provides important information about the ETS and TCA activity but the optimal concentration has to be carefully determined experimentally to avoid the well-known side effects over the ET-capacity. After the addition of Rotenone to reach ROX state we add succinate, which does not permeate the plasma membrane, to test if the plasma membrane of the cells is altered (permeability test). If we do not obtain further stimulation of the respiration we could conclude that the living cells are not permeable and remain intact.