Difference between revisions of "Template:SUIT text D022"
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SUIT-006 O2 mt D022 is a [[coupling control protocol]] for [[mitochondrial preparations]] (tissue homogenate, isolated mitochondria and permeabilized cells). Different coupling control states are assessed ([[LEAK-respiration|''L''(n)]] - [[Oxidative phosphorylation| ''P'']] - [[ET-capacity| ''E'']]) at [[Succinate pathway control state|succinate pathway control state]]. The use of [[rotenone]], that inhibits complex I, provides a specific coupling control protocol for S-pathway, avoiding [[Reverse electron flow from CII to CI|reverse electron transfer (RET)]]. | SUIT-006 O2 mt D022 is a [[coupling-control protocol]] for [[mitochondrial preparations]] (tissue homogenate, isolated mitochondria and permeabilized cells). Different coupling control states are assessed ([[LEAK-respiration|''L''(n)]] - [[Oxidative phosphorylation| ''P'']] - [[ET-capacity| ''E'']]) at [[Succinate pathway control state|succinate pathway control state]]. The use of [[rotenone]], that inhibits complex I, provides a specific coupling-control protocol for S-pathway, avoiding [[Reverse electron flow from CII to CI|reverse electron transfer (RET)]]. | ||
NOTE: In the DatLab software, the DLP file for SUIT-006 O2 mt D022 is provided without the addition of [[oligomycin]]. For using this protocol with oligomycin addition, a [[Export DL-Protocol User (*.DLPU)|DLPU]] can be created, with the Omy event and mark. Using oligomycin in the preliminary assays with a not yet studied model is useful to determine whether in this kind of sample LEAK respiration in the absence of ADP (1SRot) may be overestimated due to the presence of residual and endogenous adenylates. | NOTE: In the DatLab software, the DLP file for SUIT-006 O2 mt D022 is provided without the addition of [[oligomycin]]. For using this protocol with oligomycin addition, a [[Export DL-Protocol User (*.DLPU)|DLPU]] can be created, with the Omy event and mark. Using oligomycin in the preliminary assays with a not yet studied model is useful to determine whether in this kind of sample LEAK respiration in the absence of ADP (1SRot) may be overestimated due to the presence of residual and endogenous adenylates. | ||
Revision as of 16:30, 2 June 2020
SUIT-006 O2 mt D022 is a coupling-control protocol for mitochondrial preparations (tissue homogenate, isolated mitochondria and permeabilized cells). Different coupling control states are assessed (L(n) - P - E) at succinate pathway control state. The use of rotenone, that inhibits complex I, provides a specific coupling-control protocol for S-pathway, avoiding reverse electron transfer (RET).
NOTE: In the DatLab software, the DLP file for SUIT-006 O2 mt D022 is provided without the addition of oligomycin. For using this protocol with oligomycin addition, a DLPU can be created, with the Omy event and mark. Using oligomycin in the preliminary assays with a not yet studied model is useful to determine whether in this kind of sample LEAK respiration in the absence of ADP (1SRot) may be overestimated due to the presence of residual and endogenous adenylates.
