Template:SUIT text D016
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SUIT-009 O2 ce-pce D016 is a short protocol to investigate O2 flux in permeabilized cells (non-permeabilized cells must be added to the chambers and then permeabilized as a part of the protocol). It is recommended as a control experiment for SUIT-009 AmR ce-pce D019 without using the Amplex UltraRed assay in order to detect the effect of the fluorescence dye on the O2 flux and with the cytochrome c to assess the integrity of the mitochondrial outer membrane. We do not recommend this protocol for determination of the O2 flux in the absence of Rotenone. The following Coupling control protocol can be used to assess Succinate-linked respiration with Rotenone: SUIT-006 O2 mt D022.
