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Difference between revisions of "Template:SUIT text D016"

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SUIT-009 O2 ce-pce D016 is a short protocol to investigate O<sub>2</sub> flux in [[Permeabilized cells| permeabilized cells]] (non-permeabilized cells must be added to the chambers and then permeabilized as a part of the protocol). It is recommended as a control experiment for [[SUIT-009 AmR ce-pce D019]] without using the Amplex UltraRed assay in order to detect the effect of the fluorescence dye on the O<sub>2</sub> flux and with the cytochrome ''c'' to assess the integrity of the mitochondrial outer membrane. We do not recommend this protocol for determination of the O<sub>2</sub> flux in the absence of Rotenone. The following [[Coupling control protocol]] can be used to assess Succinate-linked respiration with Rotenone: [[SUIT-006 O2 mt D022]].
SUIT-009 O2 ce-pce D016 is a short protocol to investigate O<sub>2</sub> flux in [[Permeabilized cells| permeabilized cells]] (non-permeabilized cells must be added to the chambers and then permeabilized as a part of the protocol). It is recommended as a control experiment for [[SUIT-009 AmR ce-pce D019]] without using the Amplex UltraRed assay in order to detect the effect of the fluorescence dye on the O<sub>2</sub> flux and with the cytochrome ''c'' to assess the integrity of the mitochondrial outer membrane. We do not recommend this protocol for determination of the O<sub>2</sub> flux in the absence of Rotenone. The following [[Coupling-control protocol]] can be used to assess Succinate-linked respiration with Rotenone: [[SUIT-006 O2 mt D022]].

Latest revision as of 15:30, 2 June 2020

SUIT-009 O2 ce-pce D016 is a short protocol to investigate O2 flux in permeabilized cells (non-permeabilized cells must be added to the chambers and then permeabilized as a part of the protocol). It is recommended as a control experiment for SUIT-009 AmR ce-pce D019 without using the Amplex UltraRed assay in order to detect the effect of the fluorescence dye on the O2 flux and with the cytochrome c to assess the integrity of the mitochondrial outer membrane. We do not recommend this protocol for determination of the O2 flux in the absence of Rotenone. The following Coupling-control protocol can be used to assess Succinate-linked respiration with Rotenone: SUIT-006 O2 mt D022.