Difference between revisions of "Template:SUIT text D015"
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SUIT-009 O2 mt D015 is a short protocol for determination of O<sub>2</sub> flux in isolated mitochondria, tissue homogenate and permeabilized cells (already permeabilized when they are added to the chamber). It is recommended as a control experiment for [[SUIT-009 AmR mt D021]] without using the Amplex UltraRed assay in order to detect the effect of the fluorescent dye on the O<sub>2</sub> flux and with the cytochrome ''c'' to assess the integrity of the mitochondrial outer membrane. We do not recommend this protocol for determination of the O<sub>2</sub> flux in the absence of Rotenone. The following [[Coupling control protocol]] can be used to assess Succinate-linked respiration with Rotenone: [[SUIT-006 O2 mt D022]]. | SUIT-009 O2 mt D015 is a short protocol for determination of O<sub>2</sub> flux in isolated mitochondria, tissue homogenate and permeabilized cells (already permeabilized when they are added to the chamber). It is recommended as a control experiment for [[SUIT-009 AmR mt D021]] without using the Amplex UltraRed assay in order to detect the effect of the fluorescent dye on the O<sub>2</sub> flux and with the cytochrome ''c'' to assess the integrity of the mitochondrial outer membrane. We do not recommend this protocol for determination of the O<sub>2</sub> flux in the absence of Rotenone. The following [[Coupling-control protocol]] can be used to assess Succinate-linked respiration with Rotenone: [[SUIT-006 O2 mt D022]]. | ||
Latest revision as of 16:30, 2 June 2020
SUIT-009 O2 mt D015 is a short protocol for determination of O2 flux in isolated mitochondria, tissue homogenate and permeabilized cells (already permeabilized when they are added to the chamber). It is recommended as a control experiment for SUIT-009 AmR mt D021 without using the Amplex UltraRed assay in order to detect the effect of the fluorescent dye on the O2 flux and with the cytochrome c to assess the integrity of the mitochondrial outer membrane. We do not recommend this protocol for determination of the O2 flux in the absence of Rotenone. The following Coupling-control protocol can be used to assess Succinate-linked respiration with Rotenone: SUIT-006 O2 mt D022.
