Template:Fluorescence dyes and settings
From Bioblast
Application | Sensor | Filter set | O2k output | Light intensity (Amp polarization voltage) | Gain | Comment |
---|---|---|---|---|---|---|
AmplexTM UltraRed | Fluorescence-Sensor Green or Smart Fluo-Sensor Green | AmR | Type II | 100 - 500 | 1000 | at c(AmR) = 10 Β΅M |
TMRM | Fluorescence-Sensor Greenor Smart Fluo-Sensor Green | AmR | Type III | 100 - 500 | 1000 | at c(TMRM) = 2 Β΅M |
Safranin | Fluorescence-Sensor Blue or Smart Fluo-Sensor Blue | Saf | Type III | 100 - 250 | 1000 | at c(safranin) = 2 Β΅M |
Rhodamine 123 | Fluorescence-Sensor Blue or Smart Fluo-Sensor Blue | Saf | Type III | 100 - 250 | 1000 | at c(Rh123) = 1 Β΅M |
Magnesium Greenβ’ | Fluorescence-Sensor Blue or Smart Fluo-Sensor Blue | MgG / CaG | Type I and II | 100 - 500 | 1000 | at c(Mg Green) = 2 Β΅M |
Calcium Greenβ’ | Fluorescence-Sensor Blue or Smart Fluo-Sensor Blue | MgG / CaG | Type I | 300 - 500 | 1000 | at c(Ca Green) = 2 Β΅M |
In the O2k-LED2-Module (Series D-G) polarization voltage is used, while in the O2k-Fluo Smart Module (Series H-I) fluorescence intensity. To use the same setting in the O2k-Fluo LED2-Module as in the O2k-Fluo Smart-Module use the following Excel sheet:
Download the EXCEL calculation-sheet to adjust fluorescence intensity |