Template:Fluorescence dyes and settings: Difference between revisions
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{| class="wikitable" | :::: The light intensity of the LED ([[Fluorescence-Control_Unit#Control_of_LED-intensity|LED-intensity]]) and the signal amplification ([[Fluorescence-Control_Unit#Gain|Gain]]) can be adjusted in a wide range. The table suggests initial values, which can be optimised for specific applications. | ||
::::* The settings depend on the concentration of the fluorophore, which vary between different applications. Therefore, only recommendations for specific fluorophore concentrations are given. In the Amplexยฎย UltraRed assay the fluorophore is formed during the experiment. | |||
::::* The recommendations apply to experiments at 37 ยฐC. The Fluorescence intensity increases strongly at lower temperatures. Then the light intensity is reduced to avoid off-scale signals. | |||
{| class="wikitable" | |||
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! Application !!Sensor!! Filter set !! O2k output !! Light intensity (Amp polarization voltage)ย !! Gain !! Comment | ! Application !!Sensor!! Filter set !! O2k output !! Light intensity (Amp polarization voltage)ย !! Gain !! Comment |
Latest revision as of 13:12, 19 November 2021
- The light intensity of the LED (LED-intensity) and the signal amplification (Gain) can be adjusted in a wide range. The table suggests initial values, which can be optimised for specific applications.
- The settings depend on the concentration of the fluorophore, which vary between different applications. Therefore, only recommendations for specific fluorophore concentrations are given. In the Amplexยฎ UltraRed assay the fluorophore is formed during the experiment.
- The recommendations apply to experiments at 37 ยฐC. The Fluorescence intensity increases strongly at lower temperatures. Then the light intensity is reduced to avoid off-scale signals.
- The light intensity of the LED (LED-intensity) and the signal amplification (Gain) can be adjusted in a wide range. The table suggests initial values, which can be optimised for specific applications.
Application | Sensor | Filter set | O2k output | Light intensity (Amp polarization voltage) | Gain | Comment |
---|---|---|---|---|---|---|
AmplexTM UltraRed | Fluorescence-Sensor Green or Smart Fluo-Sensor Green | AmR | Type II | 100 - 500 | 1000 | at c(AmR) = 10 ยตM |
TMRM | Fluorescence-Sensor Green or Smart Fluo-Sensor Green | AmR | Type III | 100 - 500 | 1000 | at c(TMRM) = 2 ยตM |
Safranin | Fluorescence-Sensor Blue or Smart Fluo-Sensor Blue | Saf | Type III | 100 - 250 | 1000 | at c(safranin) = 2 ยตM |
Rhodamine 123 | Fluorescence-Sensor Blue or Smart Fluo-Sensor Blue | Saf | Type III | 100 - 250 | 1000 | at c(Rh123) = 1 ยตM |
Magnesium Greenโข | Fluorescence-Sensor Blue or Smart Fluo-Sensor Blue | MgG / CaG | Type I and II | 100 - 500 | 1000 | at c(Mg Green) = 2 ยตM |
Calcium Greenโข | Fluorescence-Sensor Blue or Smart Fluo-Sensor Blue | MgG / CaG | Type I | 300 - 500 | 1000 | at c(Ca Green) = 2 ยตM |
In the O2k-LED2-Module (Series D-G) polarization voltage is used, while in the O2k-Fluo Smart Module (Series H-I) fluorescence intensity. To use the same setting in the O2k-Fluo LED2-Module as in the O2k-Fluo Smart-Module use the following Excel sheet:
Download the EXCEL calculation-sheet to adjust fluorescence intensity |