Difference between revisions of "Talk:Oroboros FAQ"
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<span style="font-size:105%; color:#424242">'''Which are the most common applications of the [ | <span style="font-size:105%; color:#424242">'''Which are the most common applications of the [[Oxygraph-2k|OROBOROS Oxygraph-2k]] (O2k)?'''</span> | ||
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The O2k is used in basic and applied research to study the respiration of tissues, cells and mitochondria. Fairly simple to highly complex, comprehensive experimental protocols can be applied to assess either basal mitochondrial characteristics or to obtain diagnostic insight into respiratory pathways. Topically, studies using the O2k range from | The O2k is used in basic and applied research to study the respiration of tissues, cells and mitochondria. Fairly simple to highly complex, comprehensive experimental protocols can be applied to assess either basal mitochondrial characteristics or to obtain diagnostic insight into respiratory pathways. Topically, studies using the O2k range from [[O2k-Publications: Comparative MiP;environmental MiP|comparative physiological]] investigations addressing evolutionary and environmental aspects of mitochondrial metabolism over the energetics related to exercise physiology, [[O2k-Publications: Exercise physiology;nutrition;life style|nutrition and life style]], to aspects of clinical interest such as the impact of [[O2k-Publications: Mitochondrial medicine|metabolic diseases]], [[O2k-Publications: Cancer|cancer]], or [[O2k-Publications: Pharmacology;toxicology|pharmacological interventions]]. | ||
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::: '''The most prominent applications of the O2k''' | |||
:: | |||
* <span style="color:#424242">''' | ::::* <span style="color:#424242">'''Studies on exercise physiology;nutrition;life style'''</span> | ||
::» | ::::::» {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] [[MiP area::Exercise physiology;nutrition;life style]] |format=count}} [[O2k-Publications: Exercise physiology;nutrition;life style|O2k-Publications in the MiPMap]] | ||
* <span style="color:#424242">'''Diagnosis of | ::::* <span style="color:#424242">'''Diagnosis of acquired and genetic mitochondrial diseases'''</span> | ||
::» | ::::::» {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] [[Injury and adaptation::Mitochondrial Disease; Degenerative Disease and Defect]] |format=count}} [[O2k-Publications: Mitochondrial disease|O2k-Publications in the MiPMap]] | ||
* <span style="color:#424242">''' | ::::* <span style="color:#424242">'''Diagnosis of myopathies and neuromuscular pathologies'''</span> | ||
::» | ::::::» {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] [[Diseases::Myopathy]] |format=count}} [[O2k-Publications: Myopathy|O2k-Publications in the MiPMap]] | ||
* <span style="color:#424242">''' | ::::* <span style="color:#424242">'''Studies on cell function and cell death - apoptosis and necrosis'''</span> | ||
:: | ::::::» {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] [[Injury and adaptation::Cell death]] |format=count}} [[O2k-Publications: Cell death|O2k-Publications in the MiPMap]] | ||
* <span style="color:#424242">''' | ::::* <span style="color:#424242">'''Measurement of oxidative stress'''</span> | ||
::» | ::::::» {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] [[Injury and adaptation::Oxidative stress;RONS]] |format=count}} [[O2k-Publications: RONS; Oxidative stress|O2k-Publications in the MiPMap]] | ||
* <span style="color:#424242">'''Studies on | ::::* <span style="color:#424242">'''Studies on ischemia-reperfusion injury'''</span> | ||
:: | ::::::» {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] [[Injury and adaptation::Ischemia-reperfusion]] |format=count}} [[O2k-Publications: Ischemia-reperfusion|O2k-Publications in the MiPMap]] | ||
* <span style="color:#424242">''' | ::::* <span style="color:#424242">'''Studies on aging and senescence'''</span> | ||
:: | ::::::» {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] [[Diseases::Aging;senescence]] |format=count}} [[O2k-Publications: Aging; senescence|O2k-Publications in the MiPMap]] | ||
* <span style="color:#424242">''' | ::::* <span style="color:#424242">'''Pharmacological tests'''</span> | ||
::» | ::::::» {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] [[MiP area::Pharmacology;toxicology]] |format=count}} [[O2k-Publications: Pharmacology;toxicology|O2k-Publications in the MiPMap]] | ||
* <span style="color:#424242">'''For more applications, browse through the | ::::* <span style="color:#424242">'''Studies on Diabetes 2'''</span> | ||
::::::» {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] [[Diseases::Diabetes]] |format=count}} [[O2k-Publications: Diabetes 2|O2k-Publications in the MiPMap]] | |||
::::* <span style="color:#424242">'''For more applications, browse through the {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] |format=count}} [[O2k-Publications:_Topics|O2k-Publications in the MiPMap]].'''</span></div> | |||
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High-resolution respirometry is the method of choice when aiming at investigating mitochondrial function either in intact cells or various types of mitochondrial preparation. Combining instrumental accuracy and reliability with versatility of applicable protocols (allowing practically unlimited addition and combination of substrates, inhibitors and uncouplers) mitochondrial respiratory pathways may be analyzed in detail to evaluate even minor alterations in respiratory pathway control and/or capacity. | High-resolution respirometry is the method of choice when aiming at investigating mitochondrial function either in intact cells or various types of mitochondrial preparation. Combining instrumental accuracy and reliability with versatility of applicable protocols (allowing practically unlimited addition and combination of substrates, inhibitors and uncouplers) mitochondrial respiratory pathways may be analyzed in detail to evaluate even minor alterations in respiratory pathway control and/or capacity. | ||
The O2k is a sole source apparatus, with no other available instrument meeting its specifications for high-resolution respirometry. | The O2k is a sole source apparatus, with no other available instrument meeting its specifications for high-resolution respirometry. | ||
[[O2k high-resolution respirometry|High-resolution respirometry]] (HRR) is based on the O2k, combining chamber design, application of oxygen-tight materials, electrochemical and optical sensors, Peltier-temperature control and software features [[DatLab|(DatLab)]] to obtain the unique sensitive and quantitative resolution of oxygen concentration and oxygen flux, with a closed-chamber or open-chamber mode of operation. | [[O2k high-resolution respirometry|'''High-resolution respirometry''']] '''(HRR)''' is based on the O2k, combining chamber design, application of oxygen-tight materials, electrochemical and optical sensors, Peltier-temperature control and software features [[DatLab|(DatLab)]] to obtain the unique sensitive and quantitative resolution of oxygen concentration and oxygen flux, with a closed-chamber or open-chamber mode of operation. | ||
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<span style="font-size:105%; color:#424242">'''How many parameters can be measured simultaneously?'''</span> | <span style="font-size:105%; color:#424242">'''How many parameters can be measured simultaneously?'''</span> | ||
<div class="mw-collapsible-content"> The O2k is designed to measure respiration plus one additional parameter at a time. Typical applications of [[O2k-MultiSensor|O2k-MultiSensor respirometry]] are the combined measurement of respiration and H2O2 production using the [[O2k-Fluo LED2 | <div class="mw-collapsible-content"> The O2k is designed to measure respiration plus one additional parameter at a time. Typical applications of [[O2k-MultiSensor|O2k-MultiSensor respirometry]] are the combined measurement of respiration and H2O2 production using the [[O2k-Fluo LED2-Module]], of respiration and mitochondrial membrane potential with either the [[O2k-Fluo LED2-Module]] or the [[O2k-TPP+ ISE-Module|O2k-TPP+ ISE-Module]].</div> | ||
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Revision as of 15:40, 20 April 2017
TEST:
TESTSEITE
OROBOROS FAQ
Applications and samples
Which are the most common applications of the OROBOROS Oxygraph-2k (O2k)?
The O2k is used in basic and applied research to study the respiration of tissues, cells and mitochondria. Fairly simple to highly complex, comprehensive experimental protocols can be applied to assess either basal mitochondrial characteristics or to obtain diagnostic insight into respiratory pathways. Topically, studies using the O2k range from comparative physiological investigations addressing evolutionary and environmental aspects of mitochondrial metabolism over the energetics related to exercise physiology, nutrition and life style, to aspects of clinical interest such as the impact of metabolic diseases, cancer, or pharmacological interventions.
- The most prominent applications of the O2k
- Studies on exercise physiology;nutrition;life style
- Diagnosis of acquired and genetic mitochondrial diseases
- Diagnosis of myopathies and neuromuscular pathologies
- Studies on cell function and cell death - apoptosis and necrosis
- Measurement of oxidative stress
- Studies on ischemia-reperfusion injury
- Studies on aging and senescence
- Pharmacological tests
- Studies on Diabetes 2
- For more applications, browse through the 4690 O2k-Publications in the MiPMap.
What is high-resolution respirometry and what is so special about it?
High-resolution respirometry is the method of choice when aiming at investigating mitochondrial function either in intact cells or various types of mitochondrial preparation. Combining instrumental accuracy and reliability with versatility of applicable protocols (allowing practically unlimited addition and combination of substrates, inhibitors and uncouplers) mitochondrial respiratory pathways may be analyzed in detail to evaluate even minor alterations in respiratory pathway control and/or capacity. The O2k is a sole source apparatus, with no other available instrument meeting its specifications for high-resolution respirometry. High-resolution respirometry (HRR) is based on the O2k, combining chamber design, application of oxygen-tight materials, electrochemical and optical sensors, Peltier-temperature control and software features (DatLab) to obtain the unique sensitive and quantitative resolution of oxygen concentration and oxygen flux, with a closed-chamber or open-chamber mode of operation.
Is the O2k easy to use in standard applications of mitochondrial respiration?
Most 'oxygraphs' are designed for analyzing isolated mitochondria. Which types of samples can be analyzed with the O2k?
Can the O2k be applied for the measurement of oxygen consumption in delicate mammalian cells, when limited numbers of cells are available?
Can the O2k be applied for measuring human biopsies?
Can the O2k be applied to measure tissue oxygen concentration?
Can the O2k be applied for the measurement of oxygen consumption in adherent cells?
How many parameters can be measured simultaneously?
Software compatibility
Does DatLab software run on MAC devices?
O2k in comparison with other devices
Why buy an O2k and not a different oxygraph?
How and where to buy
Do you have any distributors in my country?
With the exception of the countries listed below, OROBOROS INSTRUMENTS has a direct-sales approach. This means, we generally sell our products directly to the end user. If required by the end user, it is possible to cooperate with a distributor. We cooperate with our distributors in the following countries:
- China: CN Beijing Zenda.
- Taiwan: TW Taoyuan Sunpoint.
- Republic of Korea: KR Seoul Mymed.
- Australia: AU Adelaide AIMedical.
- New Zealand: NZ Auckland GBC BioMed.
- Japan: JP Tokyo Sanyo. We are also open to requests for specific distributors as well as direct sales.
- Malaysia: MY Puchong Selangor Saujana Saintifik. We are also open to requests for specific distributors as well as direct sales.
How do I obtain price information on the O2k?
Whom do I contact to buy an O2k
Delivery content, warranty and support
How long from order till delivery?
Does the purchase include installation, setup and training?
- Installation and setup
On-site installation and setup is not included in the purchase of an O2k-Core. Setup and installation are usually performed by the customers themselves with the help of our detailed and user-friendly O2k-Manual which is sent out with the O2k. In addition, the manual can also be easily down-loaded form our website (O2k-Manual). There is also a section for technical service. Our technical specialists, based here in Innsbruck, can also assist in helping to locate and solve any technical problems, should these arise. Based on our experience and feedback received from customers all over the world, we are confident that you will easily master the set-up and installation.
- Training
Does the O2k-Core include a PC or do we have to purchase it separately?
Is it possible to extend the warranty period?
Are taxes and duty fees included in the price?
Do you offer the option to lease or rent your instruments?
Are you registered with System for Award Management (SAM)?
1. Q: Got confused with o2k-core and o2k-module? If I understood it right, o2k-core if purchased can be converted in future to measure other functions for example fluorescence, Ca+2, pH.
A: The O2k-Core is the actual instrument with the accessories, syringes and the ISS suction system. With the O2k-Core you can measure respiration, with the O2k-Modules you are able to measure different parameters as well. The Modules are ready to use with the O2k-Core without any special upgrading needed.
2. Q: How about installation of the machine in the lab here in US? Is anybody going to do it for us? Or, buyer has to do it.
A: Set-up and installation is performed by the customer with the help of our detailed and userfriendly O2k-Manual. Our customers from all over the world have been finding this very easy and our Manual is also freely available for download online. Please see: http://www.oroboros.at/index.php?o2k-manual. The manual, as well as the troubleshooting section on our wiki-based website (http://www.bioblast.at/index.php/Troubleshooting) and our technical specialists help to locate and solve any technical problems that might appear. Any parts required for repair onsite are shipped to the customer. For any grave technical faults which cannot be solved on site, the O2k is shipped to our workshop, where it is repaired.
3. Q: What is the advantage of titration-injection micropump? Is manual pipetting to add sample to the chamber difficult? How is it different from ISS-integrated suction system and microsyringe set in O2K-core?
A: TIP2k: Basically, the TIP2k makes life easier and work more comfortable, like automated determinations of oxygen background. If you are interested in e.g oxygen kinetics at low oxygen or very precise small step uncoupler titration, then the more advanced features of the TIP2k are necessary. If you want to use the O2k-Modules mentioned above were strongly recommend purchasing also the TIP2k. Most MultiSesnor measurements are also possible without it, but it gets dedious. For more information please have a look at the following link (http://www.oroboros.at/index.php?TIP2k). ISS: The ISS is for siphoning off excess medium from the O2k-Stopper and for emptying the O2k-Chambers. The ISS is included in the purchase of an O2k-Core and this suction pump is essential for the everyday usage of the instrument. The Microsyringe Set is another O2k-Core-accessory used for manual titrations.
4. Q: Are fluorescence sensors used for only green and blue emission? Is sensor not suitable for red emission? How about the excitation? Does the machine use a wide excitation spectrum?
A: You are correct, there was a confusion of excitation and emmision on our webpage. I have corrected this on http://wiki.oroboros.at/index.php/O2k-Fluorescence_LED2-Module. Thank you for bringing this to our attention!. Please note that the applications currently envisaged are listed on the top of the linked page (under description). We do not use a wide exciation but separate LED sources for the two mentioned excitation colors.
5. Q:If only Ca+2 is detected with the suitable module? Or, other ions such Na+, phosphate, K+ can also be detected.
A: I tried to answer your question here: http://wiki.oroboros.at/index.php/Talk:O2k-TPP%2B_and_Ca2%2B_ISE-Module
6. Q: Is special organ tissue or cell preparation needed to measure ions? Or, whole tissue and adherent/suspended cells are used.
A: Adherent cells (without bringing them in suspension are conceptional not well suited for our system, see http://wiki.oroboros.at/index.php/Adherent_cells. TPP+ has been measured now with three types of samples, isolated mitochondria, permabilized cultures cells and permeabilized tissues. See http://wiki.oroboros.at/index.php/Mitochondrial_membrane_potential for an overview. We have comparable little experience with Ca2+, please see http://wiki.oroboros.at/index.php/Calcium_electrode. I do not see any reason that would exclude a type of sample here.
7. Q: Where do you get NO sensor and the cost of it?
A: NO-Sensors: NO and H2O2 sensors use an amperometric measurement principle (Amp-Module), comparable to the polarographic oxygen sensor (POS), which yields an amperometric signal at a given polarization voltage. We supply custom-made stoppers that can accommodate such sensors. All amperometric sensors, like (H2S, H2O2) that have the same connection, can be used with the O2k. However, we have to mention that, while NO is measured by quite a few of our customers, the usability of any H2O2 electrode currently on the market is sometimes questioned. However, this depends on your sensitivity needs. We offer the O2k-MultiSensor Stoppers, for further information please check the following link: http://www.bioblast.at/index.php/O2k-NO_Amp-Module. For prices of NO electrodes (shaft with 2 mm diameter) please consult the manufacturer, e.g. WPI. WPI's 2 mm electrode is called "ISO-NOP" You will need the electrode housing , "replacement" sleeves #5436 (there are non with just the housing) and filling solution #7325. That is, unless they have changed the composition of their packages, so please inquire with them. For compatibility with our system the important point is the diameter of the sensor: 2mm.
8. Q: How are we going to avail parts and labor warranty in the US?
A: With our general terms and conditions we have the following warranty: 24 months* for parts and labour from day of delivery, except glassware and consumables. (*This warranty period applies in conjunction with participation in an Oxygraph-2k Course within 12 months of delivery. Otherwise the warranty period is reduced to 12 months.). To this, we can ship spare parts to you if you require any and if there is anything serious with the instrument it can be shipped to our Workshop.
9. Q: Please explain Delivery included but duty unpaid? I do not get it.
A: DDU (Delivery included - Duty Unpaid) means: We pay for the shipment (delivery) of your order and you pay for the local VAT and the customs duty for the import of the instrument.
10. Q: Different module mentioned includes o2k-chamber and oxygen sensor as spare. Do these go bad with usage? Do you recommend to have them as a backup?
A: O2k-chamber and Oxygen sensor: It is not required to have these items as backup however as they are quite essential to the usage of the instrument we recommend having them as spares. As the O2k-Chamber is out of glass, it may be good to have a backup, but not necessary. This is completely up to you. The oxygen sensor (POS) has a two year warranty and we recommend regular sensor maintenance to keep its lifetime to a maximum. POS maintenance: The sensors do not need to be shipped. For details please check our POS Service Manual which is freely available on our homepage (http://www.oroboros.at/index.php?o2k-pos-service.).
11. Q: With regard to workshop: If O2K-fluorescence is different from O2K-basic. What will be covered in the workshop in Sydney, Australia? Is registration fee included with the purchase for the workshop in Sydney?
A: The registration fee which is included with the purchase of the O2k-Core is valid for any O2k-Basic-Workshop. The Workshop in Sydney is an O2k-Basic-Workshop so you are free to go there if you prefer this destination. The registration for the Fluorescence-Workshop comes with the purchase of the Fluoresence-Module.
