Talk:O2k-Open Support alert
From Bioblast
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- Do you perform the leaky chamber test after chamber assembly?
- After chamber reassembly you might experience a small leaky chamber only identified by a leaky chamber test- Β»Leaky chamber testΒ«
- Do you perform the leaky chamber test after chamber assembly?
- Do you know that you can use the Titration-Injection microPump (TIP2k) to perform an instrumental O2 background on the O2k-sV-Module?
- By adjusting the dithionite concentration to 2.5 mM, the instrumental O2 background can be performed with specific settings for the O2k-sV-Module. - Β»O2k-sV-ModuleΒ«
- Do you know how the "switch components" approach can guide you to find what is causing your O2k not to reach the required specifications?
- Do not desperate if specifications given in the O2k-Manuals are not obtained. See how to perform an experimental troubleshooting on your own.-Β»Switch components approachΒ«
- Do you know that a humid O2k-environment and/or liquid spillage may lead to a high raw POS signal [V]?
- See how to clean and dry the electrical connections of the OroboPOS-Connector.-Β»Cleaning the electrical connectionsΒ«
- Do you know that you can use the Titration-Injection microPump (TIP2k) to perform an instrumental O2 background on the O2k-sV-Module?
O2k-Open Support alert circulars
O2k-Open Support alert circular 2020(01): in prep
- New A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.
- See how you can check that the two blue OroboPOS-Holders are intact. - Β»O2k-OroboPOS-HolderΒ«
- New A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.
- New Calibration parameters are transferred to experimental DatLab files
- Save calibration information from selected Marks while DatLab is connected to the O2k during an instrumental calibration run. Air calibration and zero oxygen calibration can be performed and saved in a single DatLab file or in separate DatLab files. - Β» Oxygen calibration - DatLabΒ«
- Do you perform O2k-Chamber volume calibration?
- The volume calibration is essential to ensure that the chamber volume is correctly calibrated to its standard volume of 2 mL (or 0.5 mL for the O2k-sV-Module). - Β»O2k-Chamber MitoPediaΒ«
- Do you use the correct O2 solubility factor (FM) in the βO2 calibrationβ window in DatLab?
- New Calibration parameters are transferred to experimental DatLab files
- The O2 solubility factor varies according to the medium used and influences the air calibration. - Β»Oxygen solubility factorΒ«