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Talk:Mitochondrial Diagnostics

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Revision as of 08:10, 18 October 2022 by Gnaiger C (talk | contribs)

                



Talk:Mitochondrial Diagnostics

Mitochondrial Diagnostics

FFG

Mitochondrial Diagnostics

  • Duration: tbc
  • Start: tbc


About Oroboros Diagnostics

  • Oroboros Diagnostics builds upon the concept of standardized functional analyses and interpretation of bioenergetic snapshots of (1) cryopreserved PBMCs obtained from minimally invasive blood samples, (2) established cell cultures subjected to pharmacological screening, and (3) mitochondrial preparations obtained from animal or human tissue samples [3,4].


Aims

  • Oroboros Diagnostics aims at establishing laboratories for mitochondrial respiratory diagnostics offering bioenergetic snapshots in collaboration with academic and clinical partners, interest groups, funding agencies, and investors.
  • Bioenergetic snapshots are obtained from substrate-uncoupler-inhibitor titration protocols in respirometric assays interrogating specific mitochondrial functions [4]. Depending on the diagnostic focus, optimized mitochondrial preparations are applied covering the entire spectrum from living or permeabilized cells to mitochondria isolated from cultured cells or tissues.


References

  1. Zdrazilova L, Hansikova H, Gnaiger E (2022) Comparable respiratory activity in attached and suspended human fibroblasts. PLoS ONE 17:e0264496.
  2. Ioannidis JPA, Greenland S, Hlatky MA, Khoury MJ, Macleod MR, Moher D, Schulz KF, Tibshirani R (2014) Increasing value and reducing waste in research design, conduct, and analysis. Lancet 383:166-75.
  3. Gnaiger E (2021) Bioenergetic cluster analysis – mitochondrial respiratory control in human fibroblasts. MitoFit Preprints 2021.8. https://doi.org/10.26124/mitofit:2021-0008
  4. Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002


Coordinator

Network

Participants

VASCage GmbH - Research Centre on Vascular Ageing and Stroke: [www.vascage.at www.vascage.at]
Oroboros Instruments GmbH - www.oroboros.at


Oroboros project involvement

Oroboros Diagnostics Events

Oroboros Diagnostics Publications

Links

News and articles


Support

This project has received funding from the Austrian Research Promotion Funding Agency (FFG).
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