Ready-made powder
For a high-altitude project in Bolivia, MiR06 had to be shipped as a complete mixture of dry powder, and be made up with deionized H2O and pH-adjusted on-site. Respiratory results were excellent in permeabilized muscle fibres with complex SUIT protocols. In contrast, BIOPS was non-functional when prepared from the mixture of dry powder. --Gnaiger Erich 20:35, 3 August 2012 (CEST)
Cost
Q: For calculating the running costs: what are the typical costs for MiR05 per run?
A: For a general discussion of running costs see 'Low O2k running costs'.
We once calculated the chemical costs for MiR05 to be about 60 € /liter (excluding VAT).
Calculated with 3 ml/per chamber (for a 2 ml chamber) this would amount to about 0.4 € per run (2 chambers).
Calculation is based on Austrian prices; and on the assumption that no "left overs" are taken into acount, i.e. the entire package is used; not taken into account are work, water, KOH for adjusting the pH ..
Fasching Mario 12:07, 13 October 2011 (CEST)
Inhibitory effects
- Lack of ADP stimulation with either glutamate&malate or succinate&rotenone excludes an explanation by rotenone retention effects.
- Do you observe the problems when using mitochondrial respiration medium (MiR05 or MiR06 with permeabilized cells), but not when using cell culture medium (intact cells)? If yes, then there could be an inhibitory compound in MiR05/MiR06. In one lab, MiR05 had to prepare always fresh: After freezing, there was an inhibition or respiration. In general, MiR05 and MiR06 (including MiR05Cr and MiR06 Cr) are perfectly OK after freezing. There must have been something in the stock chemicals that caused an inhibitory effect after freezing. Perhaps you can get a batch of medium form another lab, just to try if this medium might solve the problem?
--Gnaiger Erich 20:00, 8 November 2011 (CET)
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