Difference between revisions of "TRANSMIT"

Latest revision as of 17:53, 14 December 2020

TRANSMIT

TRANSMIT : TRANSlating the role of MItochondria in Tumorigenesis

‎

TRANSMIT: Call H2020-MSCA-ITN-2016

Marie Skłodowska-Curie Innovative Training Networks, Grant Agreement No. 722605
Duration: 48 months, extended until 2021-06-30
Start: 2017-01-01
Web: https://www.transmit-project.eu/

About TRANSMIT

TRANSMIT is a multi-partner project that intends to mobilize the critical mass of expertise, by linking beneficiaries from 7 different countries and partners from the social field. Beneficiaries are 7 world-leading basic science labs and 3 private SMEs.

Aims

Cancer is not only a genetic, but also a metabolic disease.

The consolidation of the knowledge that cancer is not only a genetic, but also a metabolic disease, has led scientists to investigate the intricate metabolic plasticity that transformed cells must undergo to survive the adverse tumor microenvironment conditions, and the contribution of oncogenes and tumor suppressors in shaping metabolism. In this scenario, genetic, biochemical and clinical evidence places mitochondria as key actors in cancer metabolic restructuring, not only because these organelles play a crucial role in energy production and formation of biosynthetic intermediates, but also because occurrence of mutations in both nuclear and mtDNA encoding metabolic enzymes is associated with different types of cancer.

TRANSMIT aims to dissect the metabolic remodeling in human cancers, placing the focus on the role of mitochondria and bridging basic research to the improvement/development of therapeutic strategies.

TRANSMIT fosters the communication of this emerging field to the patients and their families. Accordingly, TRANSMIT will create a network of seven countries, including world-leading basic science and clinical centers of excellence, several industrial partners with up-to-date technologies, as well as non-profit foundations and associations who care for cancer patients.

By creating the critical mass of scientific excellence, TRANSMIT will allow to transfer the current knowledge into the wide field of cancer research, translating scientific and technical advances to the education and training of eleven Early Stage Researchers. TRANSMIT will implement training-through-research dedicated to unravel the metabolic features of cancer, as well as to provide a full portfolio of complementary skills through the creation of a network of basic, translational and industrial laboratories, devoted to a multidisciplinary/multisectorial education of young scientists.

Objectives

TRANSMIT key methodologies and key objectives: The project is structured in three scientific WPs. For each WP, several ESRs are recruited as indicated below the WP title. Scientific WPs share the key methodologies indicated within the four sectors of the romboid in light grey. The implementation of key methodologies in pairs allows the achievement of the key objectives indicated by the dark grey arrowheads.

Coordinator

TRANSMIT project coordinator in Bologna: Anna-Maria Porcelli - Alma Mater Studiorum – Università di Bologna - IT

TRANSMIT project manager: Serena Paterlini - Alma Mater Studiorum – Università di Bologna - IT

TRANSMIT dissemination assistant: Giuseppe De Bonis - Alma Mater Studiorum – Università di Bologna - IT

Network

Project partners

Further partner organisations

Fondazione Umberto Veronesi - IT
Innova SRL - IT
CEUB Il Centro Residenziale Universitario di Bertinoro (Ce.U.B.) - IT
Dynamo Academy srl Impresa Sociale – IT

Early Stage Researchers

ESR	Name	Consortium Member	Country	Scientist in charge	Project title
1	Saharnaz Sarlak	University of Bordeaux	France	Rodrigue Rossignol	Bioenergetics of lung tumors
2	Ana Carolina Bastos Sant'Anna Silva	Oroboros Instruments	Austria	Erich Gnaiger	Cell ergometry and mitochondrial metabolic biomarkers in cancer
3	Floriana Jessica Di Paola	Justus-Liebig-University of Giessen	Germany	Sybille Mazurek	Coordination of glutaminolysis and glycolysis in cancer cells
4	Christina Schmidt	University of Cambridge	England	Christian Frezza	Fumarase and fumarate: epigenetic modifications in FH-deficient tumors
5	Nikkitha Umesh Ganesh	University of Bologna, DIMEC	Italy	Giuseppe Gasparre	Mitochondrial complex I-driven regulation of the hypoxic response in cancer cells
6	Nicole Bezuidenhout	Karolinska Institute	Sweden	Maria Shoshan	Roles of mitochondrial biogenesis enzymes in regulation of chemoresistance
7	Maheshwor Thapa	Biocrates Life Sciences AG	Austria	Guido Dallmann	Quantitative analysis of coenzymes in cancer cells
8	Ana Catarina da Silva Almeida	Université catholique de Louvain	Belgium	Pierre Sonveaux	Cancer cell models to test metabolic intervention strategies
9	Houda Abla	University of Bologna, FaBiT	Italy	Anna Maria Porcelli	Inducing pseudonormoxia as adjuvant therapeutic strategy for cancer
10	Luca Zampieri	Université catholique de Louvain	Belgium	Pierre Sonveaux	Targeting mtROS to prevent cancer metastasis
11	Daniela Weber	Paracelsus Medical University	Austria	Barbara Kofler	Impact of ketogenic diet on tumor behavior

Representative of Assembly of ESRs (AEs)

The AEs is an important body within the TRANSMIT management structure. It is composed by all ESRs recruited, in which web meetings will be held as well as during end-year meetings and compulsory training activities.

Through the AEs, the ESRs have the opportunity to discuss about your research and training activities. The AEs meetings are coordinated by a representative elected every six months by his/her own peers, for a maximum of two mandates.

Tasks of the representative:

Reports to the TRANSMIT Supervisory Board (SB) and to the Project Coordination Team (PCT) ideas, issues and suggestions of the Assembly.
Agendas and decisions of AEs meetings is filed in minutes and posted on the intranet.

Tasks of the AEs:

Organisation of end-year meetings.
Annual report on: assessment of quality and state of training; personal/social situations of ESRs; proposals for activities of individual PCDPs; proposal for activities to enhance career development; troubleshooting.

Election	ESR representative	Mandate period
21-02-2018	Ana Catarina da Silva Almeida	02-2018 to 09-2018
26-07-2018	Houda Abla	09-2018 to 02-2019
06-03-2019	Ana Catarina da Silva Almeida	03-2019 to 09-2019

Oroboros project involvement

Cell ergometry and mitochondrial metabolic biomarkers in cancer (WP1)

Compared to normal cells, cancer cells have been shown to display a reprogrammed metabolism resulting from the specific energy demands imposed by growth factor signaling. Furthermore, in the case of metastatic cells, migration and colonization of distant tissues also contribute to the extra energy burden [1]. In this regard, mitochondrial respiratory competence is retained with a shift towards biosynthetic functions, enabling cells to survive in an otherwise incompatible microenvironment and increasing the expression of key enzymes that affect the metabolic flux and proliferative pathways as well as genes involved in the acquisition of resistance to anoikis through suppression of apoptotic programs [2].

Mitochondrial NADH-linked malic enzyme 2 (ME2) does not only play a key role in glutaminolysis, but its enforced expression suppresses senescence, whereas downregulation of ME2 modulates the outcome of p53 activation, leading to strong induction of senescence but not apoptosis [3]. To assess regulatory roles, the development and application of new respiratory substrate-uncoupler-inhibitor-titration (SUIT) protocols are required due to its success in high-resolution respirometry (HRR) and cell ergometry (Pesta 2012 Methods Mol Biol). In addition, the improvement of the extension of HRR to MultiSensor analysis for simultaneous evaluation of several mitochondrial functions (e.g. mitochondrial membrane potential, reactive oxygen species production, proton flux in comparison to aerobic lactate production, Ca²⁺/ dependency) may contribute to the identification of mitochondrial metabolic biomarkers for characterizing the transformation from benign to cancer cells.

In this PhD project, the development and testing of novel biomarkers protocols will be performed initially on prostate cancer cells, in comparison with metabolic remodeling of non-cancer epithelial cells. Furthermore, the extension of the study with proteomics and metabolite tracing may contribute as a powerful tool towards metabolic flux and bioenergetics. Also, detailed analysis of the mitochondrial respiratory complexes in prostate cancer cells versus non-cancer epithelial cells as well as metastatic cells.

[1] P. S. Ward and C. B. Thompson, “Metabolic Reprogramming: A Cancer Hallmark Even Warburg Did Not Anticipate,” Cancer Cell, vol. 21, no. 3. pp. 297–308, 2012.

[2] R. J. DeBerardinis, J. J. Lum, G. Hatzivassiliou, and C. B. Thompson, “The Biology of Cancer: Metabolic Reprogramming Fuels Cell Growth and Proliferation,” Cell Metabolism, vol. 7, no. 1. pp. 11–20, 2008.

[3] P. Jiang, W. Du, A. Mancuso, K. E. Wellen, and X. Yang, “Reciprocal regulation of p53 and malic enzymes modulates metabolism and senescence,” Nature, vol. 493, no. 7434, pp. 689–693, 2013.

ESR2

Bastos Sant'Anna Silva AC, MSc was sellected to a Marie Skłodowska-Curie PhD Fellowship and enrolled her PhD at the Medical University of Innsbruck on the 1st of October, 2017, being part of Oroboros Instruments, supervised by Erich Gnaiger.

Main objective: To identify mitochondrial metabolic biomarkers for characterizing the transformation from benign to cancer cells.

Planned secondments:

University of Cambridge (3 months): To this aim she will extend her studies through metabolite tracing, since these are powerful tools towards metabolic flux and bioenergetics.

Supervision: Christian Frezza

University of Bordeaux (3 months): Detailed analysis of the mitochondrial respiratory complexes in prostate cancer cells versus non-cancer epithelial cells as well as metastatic cells.

Supervision: Rodrigue Rossignol

TRANSMIT Events

	When	Where
TRANSMIT Kick-off meeting Bologna IT	2017-02-19	Bologna IT, 2017 Feb 19 - 20 TRANSMIT Kick-off meeting.
MiPNet22.07 IOC124 Schroecken AT	2017-10-03	Schroecken AT, 2017 Oct 03-08 Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC124.
MiPNet23.01 IOC126 Innsbruck AT	2018-01-24	Innsbruck AT, 2018 Jan 24-26. Oroboros advanced O2k-Workshop on mt-membrane potential: TPP+ and fluorescent dyes. IOC126.
DSL retreat Innsbruck AT	2018-02-15	Vill AT, 2018 Feb 15. Second retreat of the Daniel Swarovski Research Laboratory (DSL).
Long Night of Research 2018 Innsbruck AT	2018-04-13	Innsbruck AT, 2018 Apr 13. Oroboros at Long Night of Research, CCB. The diagnostic bioenergetic report – a milestone on the way to mitochondrial fitness and physical well-being.
Abcam 2018 Cambridge UK	2018-06-25	Cambridge, UK, 2018 Jun 25-27. Cancer and Metabolism conference.
TRANSMIT Mid-Term Review Meeting Bologna IT	2018-11-28	Bologna, IT, 2018 Nov 28. TRANSMIT Mid-Term Review Meeting.
Course in Cancer Metabolism Bologna IT	2018-11-29	Bologna, IT, 2018 Nov 29-30. Course in Cancer Metabolism.
MiPNet23.11 IOC137 Innsbruck AT	2018-12-10	Innsbruck AT, 2018 Dec 10-12. MitoFit Coaching Days - Basic IOC137.
DSL Retreat 2019 Innsbruck AT	2019-02-21	Vill AT, 2019 Feb 21. Third retreat of the Daniel Swarovski Research Laboratory (DSL).
METABO & Cancer 2019 Marseille FR	2019-04-01	Marseille FR, 2019 Apr 1-2. 3rd edition - Metabolism and Cancer Meeting
European public and private opportunities for R&D, Innovation and Technology Transfer for TRANSMIT 2019 Rome IT	2019-05-02	Rome, IT, 2019 September 30 - October 3. European public and private opportunities for R&D, Innovation and Technology Transfer for TRANSMIT.
Course in Science Communication and Dissemination for TRANSMIT 2019 Milan IT	2019-05-02	Milan, IT, 2019 May 2-4. Course in Science Communication and Dissemination for TRANSMIT.
Course in Social Entrepreneurship for TRANSMIT 2019 Milan IT	2019-05-06	Milan, IT, 2019 May 6-7. Dynamo Academy Social Entrepreneurship and managerial skills course for TRANSMIT.
Course in Bio-statistics 2019 Bologna IT	2019-05-08	Bologna, IT, 2019 May 8-9. Course in Bio-statistics.
UNIBO Workshop 2019 Bologna IT	2019-05-13	Bologna, IT, 2019 May 13-15. Technical workshop in Genetic and bioenergetic analysis of mitochondrial DNA mutations.
6th International Conference on Tumor Microenvironment and Cellular Stress 2019 Crete GR	2019-09-23	Chania, Crete, GR, 2019 Sep 23-28. 6th International Conference on Tumor Microenvironment and Cellular Stress: Signaling, Metabolism, Imaging and Therapeutic Targets.
TRANSMIT Symposium 2020 Brussels BE	2020-01-17	Brussels, BE, 2020 Jan 17-18. TRANSMIT Symposium - Fostering applicative discoveries in cancer metabolism
The Minerva – Gentner Symposium on Cancer Immunometabolism 2020 Rehovot IL	2020-09-12	Rehovot , IL, 2020 Sep 12-14. The Minerva – Gentner Symposium on Cancer Immunometabolism 2020.
Long Night of Research 2020 Virtual Event	2020-10-09	Virtual Event, 2020 Oct 09. Oroboros at Long Night of Research, CCB. The diagnostic bioenergetic report – a milestone on the way to mitochondrial fitness and physical well-being.
TRANSMIT Open Access Course Virtual 2020	2020-10-15	Virtual, 2020 Oct 15. Open Access and Open Science
ISCaM 2020 Virtual Event	2020-10-20	Virtual Event, 2020 Oct 20-Nov 24. ISCaM2020 - 7th Annual Meeting.
TRANSMIT School 2021 Virtual	2021-03-22	Virtual Event, 2021 Mar 22-23 TRANSMIT School on respirometry and experimental protocol design, particularly related to cancer research.
TRANSMIT final meeting 2021 Virtual	2021-06-19	Virtual, 2021 Jun 19. TRANSMIT final meeting.

TRANSMIT dissemination

September 2020:

"TRANSMIT: THE ROAD TO MEET THE MITOS"- the interactive booklet for children is available for download in multiple languages- »https://www.transmit-project.eu/the-transmit-booklet-for-children-is-now-available-online-for-download/

July 2020:

“NOW” – Two worlds one goal, the TRANSMIT crowdfunding campaign to bring the world of scientific research closer to the world of patients by supporting four cancer patients’ associations. - »https://www.ideaginger.it/progetti/now.html

Events

Support

This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 722605

Official announcement of the Marie-Curie Skłodowska Action TRANSMIT: http://cordis.europa.eu/project/rcn/205395_en.html

@@ Line 1: / Line 1: @@
 {{Template:OROBOROS header page name}}
-:::: <big><big>TRANSMIT - TRANSlating the role of MItochondria in Tumorigenesis</big></big>
+<big><big>'''TRANSMIT''' : '''TRANS'''lating the role of '''MI'''tochondria in '''T'''umorigenesis</big></big>
-[[Image:3- Small Logo Acronym.jpg|right|170px|TRANSMIT]]
+[[Image:TRANSMIT Acronym.jpg|right|170px|TRANSMIT]]
 [[Image:Horizon2020_logo_jpg.jpg‎|right|150px|Horizon 2020]]
-‎__TOC__
-== TRANSMIT Marie Skłodowska-Curie PhD Fellowship with OROBOROS INSTRUMENTS / Medical University Innsbruck ==
-::::* 11 Phd student positions are open in the TRANSMIT project. OROBOROS offers one PhD position in collaboration with the Medical University of Innsbruck
-::::* Find out more, see: [[Media:Open_position_TRANSMIT_ORO.pdf|TRANSMIT PhD position]]
-::::* '''Application deadline: April 30'''
-::::* [[OROBOROS MitoFit_Laboratory |OROBOROS MitoFit Research Laboratory]]
-::::* More open positions within the TRANSMIT project can be found [http://www.biochemistry.org/JobBoard/PhDStudentships.aspx here].
+‎__TOC__
-=== WP1/ESR2: Cell ergometry and mitochondrial metabolic biomarkers in cancer ===
+== TRANSMIT: Call H2020-MSCA-ITN-2016 ==
+::::* Marie Skłodowska-Curie Innovative Training Networks, Grant Agreement No. 722605
+::::* '''Duration''': 48 months, extended  until 2021-06-30
+::::* '''Start''': 2017-01-01
+::::* '''Web''': [https://www.transmit-project.eu/ https://www.transmit-project.eu/]
-:::: After a brief period of re-emergence of the Warburg effect in oncological research, the underlying concept has been shown to be ineffective for elucidating causal relationships between mitochondrial dysfunction and tumor progression. During metabolic re-programming in many cancers and actively dividing cells mitochondrial respiratory competence is retained with a shift towards biosynthetic functions (Tan AS et al. Cell Metab 21:81-94, 2015). Mitochondrial NADH-linked malic enzyme (ME2) does not only play a key role in glutaminolysis, but its enforced expression suppresses senescence, whereas downregulation of ME2 modulates the outcome of p53 activation, leading to strong induction of senescence but not apoptosis (Jiang P, et al. Nature 493:689-93, 2013). Development, evaluation and application of new respiratory substrate-uncoupler-inhibitor-titration (SUIT) protocols are required to assess the role of ME2, which is allosterically controlled by fumarate, succinate and ATP, in mitochondrial respiratory control in cancer versus benign cells. SUIT protocols are a hallmark of the success of high-resolution respirometry and cell ergometry (Gnaiger E. 4th ed. Mitochondr Physiol Network 19.12, 2014; Pesta, D, Gnaiger E. Methods Mol Biol, 810:25-58, 2012). ESR2 will improve the extension of high-resolution respirometry to MultiSensor analysis for simultaneous evaluation of several mitochondrial functions (e.g. mitochondrial membrane potential, reactive oxygen species production, proton flux in comparison to aerobic lactate production). This approach will contribute to the identification of mitochondrial metabolic biomarkers for characterizing the transformation from benign to cancer cells. ESR2 will therefore develop and test such novel biomarker protocols initially on prostate cancer cells, in comparison with metabolic remodeling of non-cancer epithelial cells. One secondment will extend the study with proteomics and metabolite tracing. Another secondment will aim at a detailed analysis of the respiratory complexes in prostate cancer cells versus non-cancer epithelial cells.
-::::* '''Planned secondment:''' 1) CELLOMET (M25-M30, 6 months; analysis of proteomic and metabolomic profiles); 2) UNIBO (M35-M37, 3 months; analysis of respiratory complexes)
+== About TRANSMIT ==
+::::[http://www.transmit-project.eu/ '''TRANSMIT'''] is a multi-partner project that intends to mobilize the critical mass of expertise, by linking beneficiaries from 7 different countries and partners from the social field. Beneficiaries are 7 world-leading basic science labs and 3 private SMEs.
-=== Spring school ===
+[[Image:European_commission_logo.png‎|right|150px|European commission]]
-::::At M29 (June 2019 and right after the compulsory course offered by Dynamo Academy in Italy), OROBOROS will organize a Spring School entitled “The MitoFit Training Camp (MitoFit TC)” in Innsbruck (Austria) for all recruited fellows. The MitoFit Training Camp (MitoFit TC) will offer introductory and advanced lectures on experimental protocol design, development of standard operating procedures (SOPs), data analysis and reporting in mitochondrial bioenergetics and high-resolution respirometry. The MitoFit TC will provide a unique opportunity for networking with international experts in mitochondrial physiology and students. During five training days, the school will offer hands-on training in practical sessions with the O2k-Fluorometer, the state-of- the-art instrument for measuring mitochondrial respiration simultaneously with other parameters such as ROS production and mitochondrial membrane potential. The following topics will be covered specifically: laboratory standards for instrumental quality control: SOPs; reference sample for high-resolution respirometry – the MitoFit proficiency test; mitochondrial and cellular respiratory physiology; new challenges for high instrumental performance; mitochondrial respiratory coupling and substrate control. NADH and cytochrome redox states; measurement of mitochondrial membrane potential by TPP+, and fluorescent dyes; oxygen kinetics in transient states and steady-states; thermodynamic principles: flux-force relationships, power and efficiency; interpretation of diagnostic respiratory assays; comparative mitochondrial physiology: species, tissues and cell models.
+=== Aims ===
+:::: '''Cancer is not only a genetic, but also a metabolic disease.'''
-=== Relevant publications ===
-::::* Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. OROBOROS MiPNet Publications, Innsbruck: 80 pp. ISBN 978-3-9502399-8-0. [[MiPNet19.12 MitoPathways |»Bioblast link«]]
-::::* Jiang P, Du W, Mancuso A, Wellen KE, Yang X (2013) Reciprocal regulation of p53 and malic enzymes modulates metabolism and senescence. Nature 493:689-93. [[Jiang 2013 Nature|»Bioblast link«]]
-::::* Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscle. Methods Mol Biol 810:25-58. [[Pesta 2012 Methods Mol Biol|»Bioblast link«]]
-::::* Tan AS, Baty JW, Dong L, Bezawork-Geleta A, Endaya B, Goodwin J, Bajzikova M, Kovarova J, Peterka M, Yan B, Pesdar EA, Sobol M, Filimonenko A, Stuart S, Vondrusova M, Kluckova K, Sachaphibulkij K, Rohlena J, Hozak P, Truksa J, Eccles D, Haupt LM, Griffiths LR, Neuzil J, Berridge MV (2015) Mitochondrial genome acquisition restores respiratory function and tumorigenic potential of cancer cells without mitochondrial DNA. Cell Metab 21:81-94. [[Tan 2015 Cell Metab|»Bioblast link«]]
-== TRANSMIT Call H2020-MSCA-ITN-2016 ==
-[[Image:European_commission_logo.png‎|right|150px|European commission]]
-'''Cancer is not only a genetic, but also a metabolic disease'''
 :::: The consolidation of the knowledge that cancer is not only a genetic, but also a metabolic disease, has led scientists to investigate the intricate metabolic plasticity that transformed cells must undergo to survive the adverse tumor microenvironment conditions, and the contribution of oncogenes and tumor suppressors in shaping metabolism. In this scenario, genetic, biochemical and clinical evidence places mitochondria as key actors in cancer metabolic restructuring, not only because these organelles play a crucial role in energy production and formation of biosynthetic intermediates, but also because occurrence of mutations in both nuclear and mtDNA encoding metabolic enzymes is associated with different types of cancer.
-=== Objectives ===
 :::: '''TRANSMIT aims to dissect the metabolic remodeling in human cancers''', placing the focus on the role of mitochondria and bridging basic research to the improvement/development of therapeutic strategies.
@@ Line 47: / Line 33: @@
 :::: By creating the critical mass of scientific excellence, '''TRANSMIT will allow to transfer the current knowledge''' into the wide field of cancer research, translating scientific and technical advances to the education and training of eleven Early Stage Researchers. TRANSMIT will implement training-through-research dedicated to unravel the metabolic features of cancer, as well as to provide a full portfolio of complementary skills through the creation of a network of basic, translational and industrial laboratories, devoted to a multidisciplinary/multisectorial education of young scientists.
+=== Objectives ===
 :::: '''TRANSMIT key methodologies and key objectives''': The project is structured in three scientific WPs. For each WP, several ESRs are recruited as indicated below the WP title. Scientific WPs share the key methodologies indicated within the four sectors of the romboid in light grey. The implementation of key methodologies in pairs allows the achievement of the key objectives indicated by the dark grey arrowheads.
-:::: [[Image:TRANSMIT key methodologies and key objectives.jpg|750px|Key methodologies and key objectives]]
+:::::::::::::::: [[Image:TRANSMIT key methodologies and key objectives.jpg|750px|Key methodologies and key objectives]]
+== Coordinator ==
+:::: TRANSMIT project coordinator in Bologna: [[Porcelli AM|Anna-Maria Porcelli]] - [http://www.unibo.it/en/ Alma Mater Studiorum – Università di Bologna] - IT
-=== Coordinator ===
+:::: TRANSMIT project manager: [[Paterlini S|Serena Paterlini]] - [http://www.unibo.it/en/ Alma Mater Studiorum – Università di Bologna] - IT
+:::: TRANSMIT dissemination assistant: [[De Bonis G|Giuseppe De Bonis]] -  [http://www.unibo.it/en/ Alma Mater Studiorum – Università di Bologna] - IT
-:::: TRANSMIT project coordinator in Bologna:
-::::: [[Porcelli AM|Anna-Maria Porcelli]]
-::::: [http://www.unibo.it/en/ Alma Mater Studiorum – Università di Bologna] - IT
-:::: TRANSMIT project manager:
+== Network ==
-::::: [[Paterlini S|Serena Paterlini]]
-::::: [http://www.unibo.it/en/ Alma Mater Studiorum – Università di Bologna] - IT
-:::: TRANSMIT dissemination assistant:
-::::: [[De Bonis G|Giuseppe De Bonis]]
-::::: [http://www.unibo.it/en/ Alma Mater Studiorum – Università di Bologna] - IT
-:::: OROBOROS project manager:
-::::: [[Laner V|Mag. Verena Laner]]
 === Project partners ===
@@ Line 74: / Line 56: @@
 ::::*	[http://www.unibo.it/en/ Alma Mater Studiorum – Università di Bologna] - IT (lead partner)
 ::::*	[http://www.uclouvain.be/index.html/ Universite Catholique de Louvain] - BE
-::::*	[http://wiki.oroboros.at/index.php/OROBOROS_INSTRUMENTS/ OROBOROS INSTRUMENTS GmbH] - AT
+::::*	[https://www.oroboros.at/ Oroboros Instruments GmbH] - AT
 ::::*	[https://www.uni-giessen.de/welcome/ Justus Liebig Universität Giessen] - DE
 ::::*	[https://www.salk.at/ Gemeinnützige Salzburger Landeskliniken Betriebsgesellschaft]  - AT
@@ Line 81: / Line 63: @@
 ::::*	[http://www.adera.fr/ Association pour le développement de l'enseignement et des recherches aupres des universities et des enterprises d’aquitaine] - FR
 ::::*	[http://www.biocrates.com/ Biocrates Life Sciences AG] - AT
-::::*	[http://www.avanticell.com/ AvantiCell Science Ltd] - UK
 [[Image:University_of_Bologna_logo.png‎|60px|University of Bologna]]
 [[Image:Universite_Catholique_de_Louvain_logo.jpg‎‎|80px|Universite Catholique de Louvain]]
-[[Image:Logo OROBOROS INSTRUMENTS.jpg|80px|link=http://wiki.oroboros.at/index.php/OROBOROS_INSTRUMENTS OROBOROS]]
+[[Image:Logo OROBOROS INSTRUMENTS.jpg|80px|link=http://wiki.oroboros.at/index.php/OROBOROS_INSTRUMENTS Oroboros]]
 [[Image:Justus_Liebig_Universität_Giessen_logo.png‎‎‎|120px|Justus Liebig Universität Giessen]]
 [[Image:SALK_logo.png‎‎‎‎|140px|Salzburger Landeskliniken]]
@@ Line 92: / Line 73: @@
 [[Image:Adera_logo.png‎|110px|Adera]]
 [[Image:Biocrates_logo.png‎‎|130px|Biocrates Life Sciences AG]]
-[[Image:AvantiCell_Science_logo.png‎‎‎|130px|AvantiCell Science Ltd]]
 [[Image:University_of_Cambridge_logo.png‎‎‎‎‎‎|220px|University of Cambridge]]
@@ Line 108: / Line 88: @@
 [[Image:Centro_Residenziale_Universitario.png‎ |90px|Centro Residenziale Universitario die Bertinoro]]
 [[Image:Dynamo_Academy.png‎|90px|Dynamo Academy]]
+=== Early Stage Researchers ===
+:::: {| class="wikitable"
+|-
+! ESR
+! Name
+! Consortium Member
+! Country
+! Scientist in charge
+! Project title
+|-
+|  1
+| [[Sarlak S | Saharnaz Sarlak]]
+| University of Bordeaux
+| France
+| [[Rossignol R | Rodrigue Rossignol]]
+| '''Bioenergetics of lung tumors'''
+|-
+|  2
+| [[Bastos Sant'Anna Silva AC | Ana Carolina Bastos Sant'Anna Silva]]
+| [[Oroboros Instruments Corp | Oroboros Instruments]]
+| Austria
+| [[Gnaiger E | Erich Gnaiger]]
+| '''Cell ergometry and mitochondrial metabolic biomarkers in cancer'''
+|-
+|  3
+| [[Di Paola FJ | Floriana Jessica Di Paola]]
+| Justus-Liebig-University of Giessen
+| Germany
+| Sybille Mazurek
+| '''Coordination of glutaminolysis and glycolysis in cancer cells'''
+|-
+|  4
+| [[Schmidt C | Christina Schmidt]]
+| University of Cambridge
+| England
+| Christian Frezza
+| '''Fumarase and fumarate: epigenetic modifications in FH-deficient tumors'''
+|-
+|  5
+| [[Umesh Ganesh N | Nikkitha Umesh Ganesh]]
+| University of Bologna, DIMEC
+| Italy
+| [[Gasparre G | Giuseppe Gasparre]]
+| '''Mitochondrial complex I-driven regulation of the hypoxic response in cancer cells'''
+|-
+|  6
+| [[Bezuidenhout N | Nicole Bezuidenhout]]
+| Karolinska Institute
+| Sweden
+| Maria Shoshan
+| '''Roles of mitochondrial biogenesis enzymes in regulation of chemoresistance'''
+|-
+|  7
+| [[Thapa M | Maheshwor Thapa]]
+| Biocrates Life Sciences AG
+| Austria
+| Guido Dallmann
+| '''Quantitative analysis of coenzymes in cancer cells'''
+|-
+|  8
+| [[Da Silva Almeida AC | Ana Catarina da Silva Almeida]]
+| Université catholique de Louvain
+| Belgium
+| [[Sonveaux P | Pierre Sonveaux]]
+| '''Cancer cell models to test metabolic intervention strategies'''
+|-
+|  9
+| [[Abla H | Houda Abla]]
+| University of Bologna, FaBiT
+| Italy
+| [[Porcelli AM | Anna Maria Porcelli]]
+| '''Inducing pseudonormoxia as adjuvant therapeutic strategy for cancer'''
+|-
+|  10
+| [[Zampieri L | Luca Zampieri]]
+| Université catholique de Louvain
+| Belgium
+| [[Sonveaux P | Pierre Sonveaux]]
+| '''Targeting mtROS to prevent cancer metastasis'''
+|-
+|  11
+| [[Weber D | Daniela Weber]]
+| Paracelsus Medical University
+| Austria
+| [[Kofler B | Barbara Kofler]]
+| '''Impact of ketogenic diet on tumor behavior'''
+|-
+|}
+=== Representative of Assembly of ESRs (AEs) ===
+:::: The AEs is an important body within the TRANSMIT management structure. It is composed by all ESRs recruited, in which web meetings will be held as well as during end-year meetings and compulsory training activities.
+:::: Through the AEs, the ESRs have the opportunity to discuss about your research and training activities. The AEs meetings are coordinated by a representative elected every six months by his/her own peers, for a maximum of two mandates.
+:::: Tasks of the representative:
+::::* Reports to the TRANSMIT Supervisory Board (SB) and to the Project Coordination Team (PCT) ideas, issues and suggestions of the Assembly.
+::::* Agendas and decisions of AEs meetings is filed in minutes and posted on the intranet.
+:::: Tasks of the AEs:
+::::* Organisation of end-year meetings.
+::::* Annual report on: assessment of quality and state of training; personal/social situations of ESRs; proposals for activities of individual PCDPs; proposal for activities to enhance career development; troubleshooting.
+:::: {| class="wikitable"
+|-
+! Election
+! ESR representative
+! Mandate period
+|-
+| 21-02-2018
+|[[Da Silva Almeida AC | Ana Catarina da Silva Almeida]]
+| 02-2018 to 09-2018
+|-
+| 26-07-2018
+|[[Abla H | Houda Abla]]
+| 09-2018 to 02-2019
+|-
+| 06-03-2019
+|[[Da Silva Almeida AC | Ana Catarina da Silva Almeida]]
+| 03-2019 to 09-2019
+|-
+|}
+== Oroboros project involvement ==
+::::'''Cell ergometry and mitochondrial metabolic biomarkers in cancer''' (WP1)
+::::'''Compared to normal cells, cancer cells have been shown to display a reprogrammed metabolism resulting from the specific energy demands imposed by growth factor signaling. Furthermore, in the case of metastatic cells, migration and colonization of distant tissues also contribute to the extra energy burden [1]. In this regard, mitochondrial respiratory competence is retained with a shift towards biosynthetic functions, enabling cells to survive in an otherwise incompatible microenvironment and increasing the expression of key enzymes that affect the metabolic flux and proliferative pathways as well as genes involved in the acquisition of resistance to anoikis through suppression of apoptotic programs [2].
+::::Mitochondrial NADH-linked malic enzyme 2 (ME2) does not only play a key role in glutaminolysis, but its enforced expression suppresses senescence, whereas downregulation of ME2 modulates the outcome of p53 activation, leading to strong induction of senescence but not apoptosis [3]. To assess regulatory roles, the development and application of new respiratory substrate-uncoupler-inhibitor-titration (SUIT) protocols are required due to its success in high-resolution respirometry (HRR) and cell ergometry ([[Pesta 2012 Methods Mol Biol]]). In addition, the improvement of the extension of HRR to MultiSensor analysis for simultaneous evaluation of several mitochondrial functions (e.g. mitochondrial membrane potential, reactive oxygen species production, proton flux in comparison to aerobic lactate production, Ca<sup>2+</sup>/ dependency) may contribute to the identification of mitochondrial metabolic biomarkers for characterizing the transformation from benign to cancer cells.
+::::In this PhD project, the development and testing of novel biomarkers protocols will be performed initially on prostate cancer cells, in comparison with metabolic remodeling of non-cancer epithelial cells. Furthermore, the extension of the study with proteomics and metabolite tracing may contribute as a powerful tool towards metabolic flux and bioenergetics. Also, detailed analysis of the mitochondrial respiratory complexes in prostate cancer cells versus non-cancer epithelial cells as well as metastatic cells.
+::::[1] P. S. Ward and C. B. Thompson, “Metabolic Reprogramming: A Cancer Hallmark Even Warburg Did Not Anticipate,” Cancer Cell, vol. 21, no. 3. pp. 297–308, 2012.
+::::[2] R. J. DeBerardinis, J. J. Lum, G. Hatzivassiliou, and C. B. Thompson, “The Biology of Cancer: Metabolic Reprogramming Fuels Cell Growth and Proliferation,” Cell Metabolism, vol. 7, no. 1. pp. 11–20, 2008.
+::::[3] P. Jiang, W. Du, A. Mancuso, K. E. Wellen, and X. Yang, “Reciprocal regulation of p53 and malic enzymes modulates metabolism and senescence,” Nature, vol. 493, no. 7434, pp. 689–693, 2013.
+=== ESR2 ===
+:::: [[Bastos Sant'Anna Silva AC]], MSc was sellected to a Marie Skłodowska-Curie PhD Fellowship and enrolled her PhD at the Medical University of Innsbruck on the 1st of October, 2017, being part of Oroboros Instruments, supervised by [[Gnaiger E | Erich Gnaiger]].
+:::: '''Main objective:''' To identify mitochondrial metabolic biomarkers for characterizing the transformation from benign to cancer cells.
+:::: '''Planned secondments:'''
+:::* University of Cambridge (3 months): To this aim she will extend her studies through metabolite tracing, since these are powerful tools towards metabolic flux and bioenergetics.
+:::: Supervision: Christian Frezza
+:::* University of Bordeaux (3 months):  Detailed analysis of the mitochondrial respiratory complexes in prostate cancer cells versus non-cancer epithelial cells as well as metastatic cells.
+:::: Supervision: [[Rossignol R | Rodrigue Rossignol]]
+== TRANSMIT Events ==
+{{#ask:[[Category:Publications]] [[Additional label::TRANSMIT]]
+|?Was published in year=When
+|?Has title=Where
+|format=broadtable
+|limit=5000
+|offset=0
+|sort=Was published in year
+|order=ascending
+}}
+<br/>
+== TRANSMIT dissemination ==
+::: September 2020:
+::::*'''"TRANSMIT: THE ROAD TO MEET THE MITOS"'''- the interactive booklet for children is available for download in multiple languages- »https://www.transmit-project.eu/the-transmit-booklet-for-children-is-now-available-online-for-download/ <br>
+::: July 2020:
+::::* '''“NOW” – Two worlds one goal''', the TRANSMIT crowdfunding campaign to bring the world of scientific research closer to the world of patients by supporting four cancer patients’ associations. - »https://www.ideaginger.it/progetti/now.html
+::: '''Events'''
+::::* [[Bastos Sant'Anna Silva 2019 Life Sciences Meeting 2019 Innsbruck AT]]
+::::* [[Bastos Sant'Anna Silva 2018 TRANSMIT Bertinoro di Romagna IT]]
+::::* [[Bastos Sant'Anna Silva 2018 MiPschool Tromso C4]]
+::::* [[Bastos Sant'Anna Silva 2018 Life Sciences Meeting 2018 Innsbruck AT]]
+== Support  ==
+:::: This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 722605
 ::::* Official announcement of the Marie-Curie Skłodowska Action TRANSMIT: http://cordis.europa.eu/project/rcn/205395_en.html