TMRM: Difference between revisions
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|description='''TMRM''' ( tetramethylrhodamine methyl ester) is an [[extrinsic fluorophores|extrinsic fluorophore]] and can be used as a probe to determine changes in [[Mitochondrial_membrane_potential|mitochondrial membrane potential]]. TMRM is a lipophilic cations that is accumulated by mitochondria in proportion to Δ''ψ''<sub>mt</sub>. Upon accumulation of the dye it exhibits a red shift in its absorption and fluorescence emission spectrum. The fluorescence intensity is quenched when the dye is accumulated by mitochondria. | |description='''TMRM''' ( tetramethylrhodamine methyl ester) is an [[extrinsic fluorophores|extrinsic fluorophore]] and can be used as a probe to determine changes in [[Mitochondrial_membrane_potential|mitochondrial membrane potential]]. TMRM is a lipophilic cations that is accumulated by mitochondria in proportion to Δ''ψ''<sub>mt</sub>. Upon accumulation of the dye it exhibits a red shift in its absorption and fluorescence emission spectrum. The fluorescence intensity is quenched when the dye is accumulated by mitochondria. | ||
|info=[[Ehrenberg 1988 Biophys J]] | |||
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{{MitoPedia methods | {{MitoPedia methods | ||
Revision as of 10:09, 21 March 2014
- high-resolution terminology - matching measurements at high-resolution
TMRM
Description
TMRM ( tetramethylrhodamine methyl ester) is an extrinsic fluorophore and can be used as a probe to determine changes in mitochondrial membrane potential. TMRM is a lipophilic cations that is accumulated by mitochondria in proportion to Δψmt. Upon accumulation of the dye it exhibits a red shift in its absorption and fluorescence emission spectrum. The fluorescence intensity is quenched when the dye is accumulated by mitochondria.
Abbreviation: TMRM
Reference: Ehrenberg 1988 Biophys J
MitoPedia methods:
Fluorometry
The TMRM method can be used with the O2k-Fluorescence LED2-Module. For an example of the use of TMRM with mouse liver mitochondria see the discussion page of this entry
