Difference between revisions of "Smith 2015 Fatty Acid Oxidation O2k-Network Discussion Forum"
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'''Protocol''': For maximal respiration we make sequential additions of 18 µM PC, 20 µM PCoA, and 5 mM Carnitine. Addition of ADP induces a peak rate of O2 consumption that is not increased with FCCP addition. | '''Protocol''': For maximal respiration we make sequential additions of 18 µM PC, 20 µM PCoA, and 5 mM Carnitine. Addition of ADP induces a peak rate of O2 consumption that is not increased with FCCP addition. | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|preparations=Permeabilized tissue | |preparations=Permeabilized tissue | ||
|couplingstates=OXPHOS, | |couplingstates=OXPHOS, ET | ||
| | |pathways=F | ||
|additional=Palmitoylcarnitine, Palmitoyl-CoA, and L-Carnitine | |additional=Palmitoylcarnitine, Palmitoyl-CoA, and L-Carnitine | ||
}} | }} | ||
Latest revision as of 16:08, 13 November 2017
| Smith 2015 Fatty Acid Oxidation O2k-Network Discussion Forum |
Link: O2k-Network Discussion Forum
Smith CD (2015)
Event: Fatty Acid Oxidation O2k-Network Discussion Forum
I have extensively tested FAO in PMFBs using the Palmitoylcarnitine, Palmitoyl-CoA, and L-Carnitine.
Protocol: For maximal respiration we make sequential additions of 18 µM PC, 20 µM PCoA, and 5 mM Carnitine. Addition of ADP induces a peak rate of O2 consumption that is not increased with FCCP addition.
Labels:
Preparation: Permeabilized tissue
Coupling state: OXPHOS, ET
Pathway: F
Palmitoylcarnitine, Palmitoyl-CoA, and L-Carnitine
