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Difference between revisions of "Smith 2015 Fatty Acid Oxidation O2k-Network Discussion Forum"

From Bioblast
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{{Abstract
{{Abstract
|title=Smith 2015 Fatty Acid Oxidation O2k-Network Discussion Forum
|title=Smith 2015 Fatty Acid Oxidation O2k-Network Discussion Forum
|info=[http://www.bioblast.at/index.php/Talk:Fatty_acid_oxidation O2k-Network Discussion Forum]
|info=[http://www.bioblast.at/index.php/Talk:Fatty_acid_oxidation O2k-Network Discussion Forum]
|authors=Smith CD
|authors=Smith CD
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|abstract=I have extensively tested FAO in PMFBs using the Palmitoylcarnitine, Palmitoyl-CoA, and L-Carnitine.  
|abstract=I have extensively tested FAO in PMFBs using the Palmitoylcarnitine, Palmitoyl-CoA, and L-Carnitine.  


'''Protocol''': For maximal respiration we make sequential additions of 18 µM PC, 20 µM PCoA, and 5 mM Carnitine. Addition of ADP induces a peak rate of O2 consumption that is not increased with FCCP addition.
'''Protocol''': For maximal respiration we make sequential additions of 18 µM PC, 20 µM PCoA, and 5 mM Carnitine. Addition of ADP induces a peak rate of O2 consumption that is not increased with FCCP addition. Final concentrations of ADP are 1mM and for FCCP, 700nM.
 
Coupling - Because malate is not included in these protocols for anapleurotic substrate, coupling was not extensively studies using these substrate conditions, but rather focus was placed on maximal capacity to oxidize lipid under state 4 conditions.
 
Substrate States - Dissolved in H<sub>2</sub>O (PC requires slight heating to fully dissolve)
|keywords=MCK-PPARalpha, PPARalpha-null, Fld-mice
}}
}}
{{Labeling
{{Labeling
|organism=Mouse
|tissues=Skeletal muscle
|preparations=Permeabilized tissue
|preparations=Permeabilized tissue
|couplingstates=OXPHOS, ETS
|couplingstates=LEAK, OXPHOS, ETS
|substratestates=FAO
|substratestates=FAO
|additional=Palmitoylcarnitine, Palmitoyl-CoA, and L-Carnitine
|additional=Palmitoylcarnitine, Palmitoyl-CoA, and L-Carnitine
}}
}}

Revision as of 11:54, 30 November 2015

Smith 2015 Fatty Acid Oxidation O2k-Network Discussion Forum

Link: O2k-Network Discussion Forum

Smith CD (2015)

Event: Fatty Acid Oxidation O2k-Network Discussion Forum

I have extensively tested FAO in PMFBs using the Palmitoylcarnitine, Palmitoyl-CoA, and L-Carnitine.

Protocol: For maximal respiration we make sequential additions of 18 µM PC, 20 µM PCoA, and 5 mM Carnitine. Addition of ADP induces a peak rate of O2 consumption that is not increased with FCCP addition. Final concentrations of ADP are 1mM and for FCCP, 700nM.

Coupling - Because malate is not included in these protocols for anapleurotic substrate, coupling was not extensively studies using these substrate conditions, but rather focus was placed on maximal capacity to oxidize lipid under state 4 conditions.

Substrate States - Dissolved in H2O (PC requires slight heating to fully dissolve)

Keywords: MCK-PPARalpha, PPARalpha-null, Fld-mice


Labels:


Organism: Mouse  Tissue;cell: Skeletal muscle  Preparation: Permeabilized tissue 


Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property. 


Palmitoylcarnitine, Palmitoyl-CoA, and L-Carnitine