Saponin: Difference between revisions
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|description='''Saponin''' is a mild detergent that [[Permeabilization of plasma membrane|permeabilizes plasma membranes]] completely and selectively due to their high cholesterol content, whereas mt-membranes with lower cholesterol content are affected only at higher concentrations. Applied for [[Permeabilized tissue or cells|permeabilization of muscle fibres]]. | |description='''Saponin''' is a mild detergent that [[Permeabilization of plasma membrane|permeabilizes plasma membranes]] completely and selectively due to their high cholesterol content, whereas mt-membranes with lower cholesterol content are affected only at higher concentrations. Applied for [[Permeabilized tissue or cells|permeabilization of muscle fibres]]. | ||
|type=Chemicals | |type=Chemicals | ||
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{{MitoPedia methods|type=Chemicals | |||
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{{MitoPedia topics | {{MitoPedia topics | ||
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
'''Sap: Saponin''' (from quillaja bark); Sigma S 7900, 25 g, store at | '''Sap: Saponin''' (from quillaja bark); Sigma S 7900, 25 g, store at RT. | ||
'''Comment:''' ''Saponin from Quillaja Bark has the product numbers S 7900 and S 2149, but S 2149 is withdrawn from Sigma | '''Comment:''' ''Saponin from Quillaja Bark has the product numbers S 7900 and S 2149, but S 2149 is withdrawn from Sigma''. | ||
<span style="color:#8B008B"> '''Caution:''' Prepare fresh everyday!</span> | <span style="color:#8B008B"> '''Caution:''' Prepare fresh everyday!</span> | ||
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'''Preparation of 5 mg/ml stock solution (dissolved in H<sub>2</sub>O)''': | '''Preparation of 5 mg/ml stock solution (dissolved in H<sub>2</sub>O)''': | ||
::1) | ::1) Weigh 5 mg of saponin. | ||
::2) | ::2) Dissolve in 1 ml H<sub>2</sub>O. | ||
→ | → Use 50 µg saponin/ml [[Biopsy preservation solution|BIOPS]]: add 20 µl of 5 mg/ml saponin stock solution per 2 ml BIOPS. | ||
Revision as of 14:41, 30 December 2013
- high-resolution terminology - matching measurements at high-resolution
Saponin
Description
Saponin is a mild detergent that permeabilizes plasma membranes completely and selectively due to their high cholesterol content, whereas mt-membranes with lower cholesterol content are affected only at higher concentrations. Applied for permeabilization of muscle fibres.
Abbreviation: Sap
MitoPedia topics: Permeabilization agent
Application in HRR
Sap: Saponin (from quillaja bark); Sigma S 7900, 25 g, store at RT.
Comment: Saponin from Quillaja Bark has the product numbers S 7900 and S 2149, but S 2149 is withdrawn from Sigma.
Caution: Prepare fresh everyday!
Preparation of 5 mg/ml stock solution (dissolved in H2O):
- 1) Weigh 5 mg of saponin.
- 2) Dissolve in 1 ml H2O.
→ Use 50 µg saponin/ml BIOPS: add 20 µl of 5 mg/ml saponin stock solution per 2 ml BIOPS.
