Difference between revisions of "Saponin"

Latest revision as of 15:42, 4 April 2024

high-resolution terminology - matching measurements at high-resolution

Saponin

Description

Saponin is a mild detergent that permeabilizes plasma membranes completely and selectively due to their high cholesterol content, whereas mt-membranes with lower cholesterol content are affected only at higher concentrations. Applied for permeabilization of muscle fibres.

Abbreviation: Sap

Reference: Doerrier 2018 Methods Mol Biol

MitoPedia topics: Permeabilization agent

Application in HRR

Sap: Saponin (from quillaja bark); Sigma S7900, 25 g, store at RT.

Comment: Saponin from Quillaja Bark has the product numbers S7900 and S2149, but S2149 is withdrawn from Sigma.

Caution: Prepare fresh everyday!

Preparation of 5 mg/mL stock solution (dissolved in BIOPS):

1) Weigh 5 mg of saponin.

2) Dissolve in 1 mL BIOPS.

→ Use 50 µg saponin/mL BIOPS: add 20 µL of 5 mg/mL saponin stock solution per 2 mL BIOPS.

@@ Line 2: / Line 2: @@
 |abbr=Sap
 |description='''Saponin''' is a mild detergent that [[Permeabilization of plasma membrane|permeabilizes plasma membranes]] completely and selectively due to their high cholesterol content, whereas mt-membranes with lower cholesterol content are affected only at higher concentrations.  Applied for [[Permeabilized tissue or cells|permeabilization of muscle fibres]].
-|type=Chemicals
+|info=[[Doerrier 2018 Methods Mol Biol]]
 }}
+{{MitoPedia concepts}}
+{{MitoPedia methods}}
+{{MitoPedia O2k and high-resolution respirometry}}
 {{MitoPedia topics
 |mitopedia topic=Permeabilization agent
-|type=Chemicals
 }}
+== Application in [[HRR]] ==
+'''Sap: Saponin''' (from quillaja bark); Sigma S7900, 25 g, store at RT.
-== Application in [[HRR]] ==
+'''Comment:''' ''Saponin from Quillaja Bark has the product numbers S7900 and S2149, but S2149 is withdrawn from Sigma''.
-'''Sap: Saponin''' (from quillaja bark); Sigma S 2149, 25 g, store at R.T.; FW = 1229.31
 <span style="color:#8B008B"> '''Caution:''' Prepare fresh everyday!</span>
-'''Comment:''' ''The product is withdrawn from Sigma!''
+'''Preparation of 5 mg/mL stock solution (dissolved in''' [[Biopsy preservation solution|BIOPS]]):
+::1) Weigh 5 mg of saponin.
+::2) Dissolve in 1 mL BIOPS.
-'''Preparation of 5 mg/ml stock solution (dissolved in H<sub>2</sub>O)''':
-::1) weigh 5 mg of Saponin
-::2) dissolve in 1 ml H<sub>2</sub>O
-→ use 50 µg Saponin/ml [[Biopsy preservation solution|BIOPS]]: add 20 µl of 5 mg/ml saponin stock solution per 2 ml BIOPS
+→ Use 50 µg saponin/mL [[Biopsy preservation solution|BIOPS]]: add 20 µL of 5 mg/mL saponin stock solution per 2 mL BIOPS.