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Revision as of 13:09, 3 March 2021 by Spitzer Gudrun (talk | contribs) (added instructions for preparation of higher concentration plus hint, that EtOH could be used, too)
Description
Salicylhydroxamic acid (SHAM; synonym: 2-Hydroxybenzohydroxamic acid N,2-Dihydroxybenzamide) is an inhibitor of the alternative oxidase (AOX). When AOX is blocked by SHAM, electrons are forced through the CIII-cytochrome c oxidase pathway, allowing observation of the operation of the CIII-CIV pathway without AOX activity.
Abbreviation: SHAM
Reference: Parrino_2000_J_Exp_Biol
Application in HRR
- SHAM: Salicylhydroxamic acid (99%; HOC6H4CONHOH); Sigma S 607, 5 g, store at RT; M = 153.14 g·mol-1.
- SHAM is reported to be easily soluble in EtOH. So EtOH could be considered as a solvent, too. However, in Oroboros-lab EtOH has not been tested as a solvent yet.
- Caution: Irritant!
- Preparation of 250 mM stock solution (dissolved in DMSO):
- Weigh 76.57 mg of SHAM.
- Dissolve in 1950 µL of DMSO.
- Divide into 0.2 mL portions
- Store at -20 °C.
- Preparation of 250 mM stock solution (dissolved in DMSO):
- » O2k Manual Titrations: MiPNet09.12 O2k-Titrations
- Titration volume: 8 µL
- Final conc. in 2 mL O2k chamber: 1 mM
MitoPedia topics:
Inhibitor