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Difference between revisions of "SUITbrowser"

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{{MitoPedia
{{Technical support}}
|description=Use the '''SUITbrowser''' to find the best substrate-uncoupler-inhibitor-titration ([[SUIT]]) protocol for your research questions.
{{MitoPedia without banner
|description=Use the '''SUITbrowser''' to find the substrate-uncoupler-inhibitor-titration ([[SUIT]]) protocol most suitable for addressing your research questions.


Β  Open the SUITbrowser: http://suitbrowser.oroboros.at/
Β  <big><big>Open the SUITbrowser: http://suitbrowser.oroboros.at/</big></big>


Each SUIT protocol receives a score from 0 to 3 in different categories that correspond to a research question (e.g. information on coupling control, information on fatty acid oxidation, among others). This score is set depending on the information which one can get from each SUIT protocol (3: this SUIT protocol is ideal, highly informative for the specific question; 2: information provided; 1: information provided, but is not a SUIT protocol mainly focused for the study of this question; 0: the specific information/question is not provided by the SUIT protocol). It is possible to choose multiple research questions at a time, to find the best protocols that address this combination of problems.
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[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=8T33sp9KkJk]] [https://www.youtube.com/watch?v=8T33sp9KkJk How to find a DL-Protocol (DLP)]
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{{NextGen-O2k H2020-support}}
{{Technical support integrated}}
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:::: Each SUIT protocol receives a score from 0 to 3 in different categories that correspond to a research question (e.g. information on coupling control, information on fatty acid oxidation, among others). This score is set depending on the information which one can get from each SUIT protocol (3: this SUIT protocol is ideal, highly informative for the specific question; 2: information provided; 1: information provided, but is not a SUIT protocol mainly focused for the study of this question; 0: the specific information/question is not provided by the SUIT protocol). It is possible to choose multiple research questions at a time, to find the best protocols that address this combination of problems.
== Define your experimental strategy ==
== Define your experimental strategy ==


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::::* [[Coupling control state|Coupling control]]
::::* [[Coupling-control state|Coupling control]]


::::* [[Oxidative phosphorylation|OXPHOS Respiration]]
::::* [[Oxidative phosphorylation|OXPHOS respiration]]


::::* [[ROUTINE-respiration|ROUTINE Respiration]]
::::* [[ROUTINE respiration]]


::::* [[NS-pathway_control_state#SUITbrowser_question:_Maximum_physiological_capacity|Maximum physiological capacity]]
::::* [[NS-pathway_control_state#SUITbrowser_question:_Maximum_physiological_capacity|Maximum physiological capacity]]
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::::* [[Alternative oxidase|AOX activity]]
::::* [[Alternative oxidase|AOX activity]]


::::* [[MitoKit-CII MitoPedia|Permeable succinate/malonate]]
::::* [[MitoKit-CII|Permeable succinate/malonate]]


::::* [[Carbohydrate]]
::::* [[Carbohydrate]]
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::::* [[Fatty acid oxidation]]
::::* [[Fatty acid oxidation]]


::::* [[Glutamate anaplerotic pathway control state|Glutamine/glutamate]]
::::* [[Glutamate-anaplerotic pathway control state|Glutamine/glutamate]]


::::* [[Succinate|Succinate pathway]]
::::* [[Succinate|Succinate pathway]]
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|mitopedia O2k and high-resolution respirometry=DatLab
|mitopedia O2k and high-resolution respirometry=DatLab
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[[Category:SUITbrowser]]
[[Category:SUITbrowser]]

Latest revision as of 05:51, 3 November 2022


                  


O2k-Open Support

SUITbrowser



Template NextGen-O2k.jpg


MitoPedia O2k and high-resolution respirometry: O2k-Open Support 



Description

Use the SUITbrowser to find the substrate-uncoupler-inhibitor-titration (SUIT) protocol most suitable for addressing your research questions.

Open the SUITbrowser: http://suitbrowser.oroboros.at/


PlayVideo.jpg How to find a DL-Protocol (DLP)




Template NextGen-O2k.jpg


MitoPedia O2k and high-resolution respirometry: O2k-Open Support 



Each SUIT protocol receives a score from 0 to 3 in different categories that correspond to a research question (e.g. information on coupling control, information on fatty acid oxidation, among others). This score is set depending on the information which one can get from each SUIT protocol (3: this SUIT protocol is ideal, highly informative for the specific question; 2: information provided; 1: information provided, but is not a SUIT protocol mainly focused for the study of this question; 0: the specific information/question is not provided by the SUIT protocol). It is possible to choose multiple research questions at a time, to find the best protocols that address this combination of problems.

Define your experimental strategy

Mitochondrial preparations or living cells

  1. Mitochondrial preparations: imt, thom, pce, pti (including pfi)
  2. Living cells: ce = vce + dce

Applications

  1. O2
  2. H2O2 and O2
  3. Mitochondrial ATP production and O2
  4. Membrane potential and O2


Research questions

The following research questions can be selected in the SUITbrowser in various combinations to provide links to the best SUIT protocols:




MitoPedia concepts: SUIT concept, SUIT protocol 


MitoPedia methods: Respirometry, Fluorometry 


MitoPedia O2k and high-resolution respirometry: DatLab