Difference between revisions of "SUITbrowser"
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{{MitoPedia | {{Technical support}} | ||
|description=Use the '''SUITbrowser''' to find the | {{MitoPedia without banner | ||
|description=Use the '''SUITbrowser''' to find the substrate-uncoupler-inhibitor-titration ([[SUIT]]) protocol most suitable for addressing your research questions. | |||
Open the SUITbrowser: http://suitbrowser.oroboros.at/ | <big><big>Open the SUITbrowser: http://suitbrowser.oroboros.at/</big></big> | ||
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=8T33sp9KkJk]] [https://www.youtube.com/watch?v=8T33sp9KkJk How to find a DL-Protocol (DLP)] | |||
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{{ | {{Technical support integrated}} | ||
__TOC__ | __TOC__ | ||
:::: Each SUIT protocol receives a score from 0 to 3 in different categories that correspond to a research question (e.g. information on coupling control, information on fatty acid oxidation, among others). This score is set depending on the information which one can get from each SUIT protocol (3: this SUIT protocol is ideal, highly informative for the specific question; 2: information provided; 1: information provided, but is not a SUIT protocol mainly focused for the study of this question; 0: the specific information/question is not provided by the SUIT protocol). It is possible to choose multiple research questions at a time, to find the best protocols that address this combination of problems. | |||
== Define your experimental strategy == | == Define your experimental strategy == | ||
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::::* [[Coupling control state|Coupling control]] | ::::* [[Coupling-control state|Coupling control]] | ||
::::* [[Oxidative phosphorylation|OXPHOS respiration]] | |||
::::* [[ROUTINE respiration]] | |||
::::* [[NS-pathway_control_state#SUITbrowser_question:_Maximum_physiological_capacity|Maximum physiological capacity]] | |||
::::* [[Living cells|Cell viability test]] | |||
::::* [[Crabtree effect]] | |||
::::* [[ | ::::* [[Alternative oxidase|AOX activity]] | ||
::::* [[MitoKit-CII|Permeable succinate/malonate]] | |||
::::* [[Carbohydrate]] | ::::* [[Carbohydrate]] | ||
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::::* [[Fatty acid oxidation]] | ::::* [[Fatty acid oxidation]] | ||
::::* [[Glutamate anaplerotic pathway control state|Glutamine/glutamate | ::::* [[Glutamate-anaplerotic pathway control state|Glutamine/glutamate]] | ||
::::* [[Succinate|Succinate pathway]] | ::::* [[Succinate|Succinate pathway]] | ||
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::::* [[Q-junction|Q-junction (FNSGp)]] | ::::* [[Q-junction|Q-junction (FNSGp)]] | ||
::::* [[Complex_IV#SUITbrowser_question:_CIV|Complex IV | ::::* [[Complex_I-linked_substrate_state#SUITbrowser_question:_CI_threshold|CI threshold]] | ||
::::* [[Complex_IV#SUITbrowser_question:_CIV|Complex IV activity]] | |||
::::* [[Cytochrome_c#SUITbrowser_question:_mt_outer_membrane_integrity|mt outer membrane integrity]] | ::::* [[Cytochrome_c#SUITbrowser_question:_mt_outer_membrane_integrity|mt outer membrane integrity]] | ||
::::* [[ | ::::* [[ATPases|Unspecific ATPase activity]] | ||
::::* [[Digitonin|Optimum digitonin concentration]] | |||
::::* [[Magnesium Green|Mitochondrial ATP production]] | |||
::::* [[Mitochondrial membrane potential]] | |||
::::* [[Hydrogen peroxide|H<sub>2</sub>O<sub>2</sub> - membrane potential dependence]] | |||
::::* [[Hydrogen peroxide|H<sub>2</sub>O<sub>2</sub> - [O<sub>2</sub>] dependence]] | |||
::::* [[Hydrogen peroxide|H<sub>2</sub>O<sub>2</sub> - RET initiated]] | |||
::::* [[Hydrogen peroxide|H<sub>2</sub>O<sub>2</sub> - NADH-pathway initiated]] | |||
{{MitoPedia concepts | {{MitoPedia concepts | ||
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|mitopedia O2k and high-resolution respirometry=DatLab | |mitopedia O2k and high-resolution respirometry=DatLab | ||
}} | }} | ||
[[Category:SUITbrowser]] | [[Category:SUITbrowser]] | ||
Latest revision as of 06:51, 3 November 2022
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SUITbrowser |
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
Description
Use the SUITbrowser to find the substrate-uncoupler-inhibitor-titration (SUIT) protocol most suitable for addressing your research questions.
Open the SUITbrowser: http://suitbrowser.oroboros.at/
How to find a DL-Protocol (DLP)
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
- Each SUIT protocol receives a score from 0 to 3 in different categories that correspond to a research question (e.g. information on coupling control, information on fatty acid oxidation, among others). This score is set depending on the information which one can get from each SUIT protocol (3: this SUIT protocol is ideal, highly informative for the specific question; 2: information provided; 1: information provided, but is not a SUIT protocol mainly focused for the study of this question; 0: the specific information/question is not provided by the SUIT protocol). It is possible to choose multiple research questions at a time, to find the best protocols that address this combination of problems.
Define your experimental strategy
Mitochondrial preparations or living cells
- Mitochondrial preparations: imt, thom, pce, pti (including pfi)
- Living cells: ce = vce + dce
Applications
- O2
- H2O2 and O2
- Mitochondrial ATP production and O2
- Membrane potential and O2
Research questions
- The following research questions can be selected in the SUITbrowser in various combinations to provide links to the best SUIT protocols:
MitoPedia concepts:
SUIT concept,
SUIT protocol
MitoPedia methods:
Respirometry,
Fluorometry
MitoPedia O2k and high-resolution respirometry:
DatLab
