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Difference between revisions of "SUIT-031 Q ce-pce D074"

From Bioblast
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{{MitoPedia
|abbr= PM+S+Rot
|description=[[File:Ce1;1Dig;1PM;2D;3S;4Rot;5U;5Anoxia;6Ama.png|400px]]
|info='''A'''Β  to analyse O2 flux and the redox state of the Q-pool in the NS- pathway control state on permeabilized cells- '''[[SUIT-031]]'''
|application=Q
|SUIT number=D074_ce1;1Dig;1PM;2D;3S;4Rot;5U;5Anoxia;6Ama
}}
{{Template:SUIT text D074}}
''in preparation''
__TOC__
Communicated by [[Komlodi T]] (last update 2021-02-01)


== Representative traces ==
[[File:SUIT-031 Q ce-pce D074 O2.png|700px]]
[[File:SUIT-031 Q ce-pce D074 Q.png|700px]]
{{Template:SUIT-031 Q ce-pce D074}}
== Strengths and limitations ==
:::*
:::+ Reasonable duration of the experiment.
:::- This protocol cannot be extended with the Complex IV module.
:::- Careful washing is required after the experiment to avoid carry-over of uncoupler and inhibitors, if used.
== Compare SUIT protocols ==
== Chemicals and syringes ==
{{Template:Chemicals ce}}
{{Template:Chemicals Q}}
{{Template:Chemicals SUIT-031 Q mt D072}}
::: Suggested stock concentrations are shown in the specific DL-Protocol.
== References ==
{{#ask:[[Category:Publications]] [[Additional label::SUIT-031 Q mt D072]]
| ?Was published in year=Year
| ?Has title=Reference
| ?Mammal and model=Organism
| ?Tissue and cell=Tissue;cell
| format=broadtable
| limit=5000
| offset=0
| sort=Was published in year
| order=descending
}}
{{MitoPedia concepts
|mitopedia concept=SUIT protocol, SUIT A, Find
}}
{{MitoPedia methods
|mitopedia method=Respirometry, Potentiometry
}}

Revision as of 15:16, 1 February 2021