Difference between revisions of "SUIT-006 O2 ce-pce D029"
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|SUIT number=D029_Ce1;1Dig;1PM;2D;2c;3U;4Ama | |SUIT number=D029_Ce1;1Dig;1PM;2D;2c;3U;4Ama | ||
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::: '''[[Categories of SUIT protocols |SUIT-category]]:''' N | ::: '''[[Categories of SUIT protocols |SUIT-category]]:''' N(PM) | ||
::: '''[[SUIT protocol pattern]]:''' linear [[coupling control protocol]] Ce1;1Dig;1PM;2D;2c;3U;4Ama | ::: '''[[SUIT protocol pattern]]:''' linear [[coupling control protocol]] Ce1;1Dig;1PM;2D;2c;3U;4Ama | ||
::::* Linked to | ::::* Linked to | ||
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== Strengths and limitations == | == Strengths and limitations == | ||
:::* This protocol is indicated when the aim is to analyse coupling control in | :::* This protocol is indicated when the aim is to analyse coupling control in permeabilized cells at N(PM) [[electron transfer-pathway state]]. | ||
:::+ Short duration of the experiment. | :::+ Short duration of the experiment. | ||
:::+ CIV activity can be measured as an extension. | :::+ CIV activity can be measured as an extension. | ||
:::- This protocol analyses coupling control at N-pathway, however, it is less specific than a similar protocol containing the complex II inhibitor malonic acid, that would allow to analyse the N-pathway without any contribution of the S-pathway. | |||
:::- This protocol does not include internal ET-pathway control steps. | :::- This protocol does not include internal ET-pathway control steps. | ||
:::- Careful washing is required after the experiment to avoid carry-over of uncoupler and inhibitors. | :::- Careful washing is required after the experiment to avoid carry-over of uncoupler and inhibitors, if used. | ||
== Compare SUIT protocols == | == Compare SUIT protocols == | ||
::::* [[SUIT-006]]: Extended protocol with SUIT-006 as the initial coupling control module. | ::::* [[SUIT-006]]: Extended protocol with SUIT-006 as the initial coupling control module. | ||
::::* [[SUIT-003]]: Coupling control protocol for non-permeabilized cells (ce), counterpart to SUIT-006 for mt-preparations (mtprep). | ::::* [[SUIT-003]]: Coupling control protocol for non-permeabilized cells (ce), counterpart to SUIT-006 for mt-preparations (mtprep). |
Revision as of 16:19, 22 January 2019
Description
Abbreviation: N(PM)
Reference: A
SUIT number: D029_Ce1;1Dig;1PM;2D;2c;3U;4Ama
O2k-Application: O2
- SUIT-category: N(PM)
- SUIT protocol pattern: linear coupling control protocol Ce1;1Dig;1PM;2D;2c;3U;4Ama
- Linked to
References
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
ce1 | ROUTINE | ce1
| ||
1Dig | REN | ce1;1Dig
|
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M)
|
---|---|---|---|---|
1PM | PML(n) | N | CI | 1PM
|
2D | PMP | N | CI | 1PM;2D
|
2c | PMcP | N | CI | 1PM;2D;2c
|
3Omy | PMLOmy | N | CI | 1PM;2D;2c;3Omy
|
4U | PME | N | CI | 1PM;2D;2c;3Omy;4U
|
5Ama | ROX | 1PM;2D;2c;3Omy;4U;5Ama
|
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Pathway control
- Main fuel substrates
- » Glutamate, G
- » Glycerophosphate, Gp
- » Malate, M
- » Octanoylcarnitine, Oct
- » Pyruvate, P
- » Succinate, S
- Main fuel substrates
- Glossary
Strengths and limitations
- This protocol is indicated when the aim is to analyse coupling control in permeabilized cells at N(PM) electron transfer-pathway state.
- + Short duration of the experiment.
- + CIV activity can be measured as an extension.
- - This protocol analyses coupling control at N-pathway, however, it is less specific than a similar protocol containing the complex II inhibitor malonic acid, that would allow to analyse the N-pathway without any contribution of the S-pathway.
- - This protocol does not include internal ET-pathway control steps.
- - Careful washing is required after the experiment to avoid carry-over of uncoupler and inhibitors, if used.
Compare SUIT protocols
MitoPedia concepts: SUIT protocol, SUIT A, Find
MitoPedia methods:
Respirometry