SUIT-003 O2 ce D028: Difference between revisions
From Bioblast
m (Komlodi Timea moved page 1R;2Omy;3U-4Rot;5S;6Ama to 1R;2Omy;3U;4Rot;5S;6Ama) |
No edit summary |
||
Line 16: | Line 16: | ||
[[File:CCP(S)04(R,L,E).jpg|200px]] In the diagrams of coupling control protocols for intact cells (CCP), the steps may be replaced by the respiratory states. | [[File:CCP(S)04(R,L,E).jpg|200px]] In the diagrams of coupling control protocols for intact cells (CCP), the steps may be replaced by the respiratory states. | ||
:::* '''3Rot,4S | :::* '''3Rot,4S;5Ama''' Β | ||
:::: '''3Rot''': In many intact cells ROX measured after titration of rotenone (3Rot) is not or only slightly further inhibited by subsequent titration of animycin A ([[CCP02|CCP02-Rot,Ama]]. | :::: '''3Rot''': In many intact cells ROX measured after titration of rotenone (3Rot) is not or only slightly further inhibited by subsequent titration of animycin A ([[CCP02|CCP02-Rot,Ama]]. | ||
:::: '''4S''': Succinate is not permeable through the plasma membrane of many intact cells ([[Steinlechner-Maran 1997 Transplantation]]). If succinate is accessible to rotenone-inhibited uncoupled mitochondria, respiration will be stimulated. | :::: '''4S''': Succinate is not permeable through the plasma membrane of many intact cells ([[Steinlechner-Maran 1997 Transplantation]]). If succinate is accessible to rotenone-inhibited uncoupled mitochondria, respiration will be stimulated. | ||
:::: '''5Ama''': Inhibition of CIII by antimycin A inhibits succinate-stimulated respiration ([[Stadlmann_2006_Cell Biochem Biophys]]). | :::: '''5Ama''': Inhibition of CIII by antimycin A inhibits succinate-stimulated respiration ([[Stadlmann_2006_Cell Biochem Biophys]]). |
Revision as of 15:40, 15 March 2017
Description
Abbreviation: RLE(S)
Reference: A CCP_RP2
MitoPedia concepts:
SUIT protocol,
SUIT A
- SUIT-catg: CCP(S)
- SUIT protocol pattern: linear coupling control protocol (CCP)
- Mark names in DatLab: CCP(S)R2
- Laboratory titration sheet: Β»SUIT CCP(S) R2
- DatLab-Analysis template: in prep.: CCP(S)R2.xlsx
R,L,E format
In the diagrams of coupling control protocols for intact cells (CCP), the steps may be replaced by the respiratory states.
- 3Rot,4S;5Ama
- 3Rot: In many intact cells ROX measured after titration of rotenone (3Rot) is not or only slightly further inhibited by subsequent titration of animycin A (CCP02-Rot,Ama.
- 4S: Succinate is not permeable through the plasma membrane of many intact cells (Steinlechner-Maran 1997 Transplantation). If succinate is accessible to rotenone-inhibited uncoupled mitochondria, respiration will be stimulated.
- 5Ama: Inhibition of CIII by antimycin A inhibits succinate-stimulated respiration (Stadlmann_2006_Cell Biochem Biophys).