Difference between revisions of "SUIT-003 Ce1;ce2SD;ce3Omy;ce4U-"
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:::: '''[[SUIT protocol pattern]]:''' complex [[coupling control protocol]] (CCP) | :::: '''[[SUIT protocol pattern]]:''' complex [[coupling control protocol]] (CCP) | ||
== Coupling control protocol == | |||
{| class="wikitable" border="1" | {| class="wikitable" border="1" | ||
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|- | |- | ||
| 1SD | | 1SD | ||
| R | | ''R''+S<sub>''P''</sub> | ||
| [[ROUTINE]] | | [[ROUTINE]] (''R'') + [[S]] (S<sub>''P''</sub>) | ||
| pce are in state S<sub>''P''</sub>. | | pce are in state S<sub>''P''</sub>. | ||
|- | |- | ||
| 2Omy | | 2Omy | ||
| | | ''L'' + S<sub>''L''</sub> | ||
| [[ | | [[LEAK]] (''L'') + [[S]] (S<sub>''L''</sub>) | ||
| ce and pce are in the LEAK state. | | ce and pce are in the LEAK state. | ||
|- | |- | ||
| 3U | | 3U | ||
| E | | ''E''+S<sub>''E''</sub> | ||
| [[ETS]] | | [[ETS]] (''E'') + [[S]] (S<sub>''E''</sub>) | ||
| ce and pce are in the ETS state. | | ce and pce are in the ETS state. | ||
|- | |- | ||
| 4Rot | | 4Rot | ||
| S<sub>''E''</sub> | | ROX+S<sub>''E''</sub> | ||
| [[ROX]] | | [[ROX]]+[[S]] (S<sub>''E''</sub>) | ||
| ce are in the ROX state, pce are in the ETS state. | | ce are in the ROX state, pce are in the ETS state. | ||
Revision as of 10:23, 29 August 2016
- high-resolution terminology - matching measurements at high-resolution
SUIT-003 Ce1;ce2SD;ce3Omy;ce4U-
Description
SUIT-catg: CC+S
Mark names in DatLab: Stadlmann 2006 Cell Biochem Biophys
DatLab-Excel template: in prep.: Stadlmann 2006 Cell Biochem Biophys.xlsx
Abbreviation: CC_R 1Omy 2U 3Rot 4S 5Ama
Reference: Stadlmann 2006 Cell Biochem Biophys
MitoPedia concepts:
SUIT protocol
- SUIT protocol pattern: complex coupling control protocol (CCP)
Coupling control protocol
| Step | Respiratory state | Pathway control | Comment |
|---|---|---|---|
| R | ROUTINE | ce | pce without substrate are in a state of ROX. |
| 1SD | R+SP | ROUTINE (R) + S (SP) | pce are in state SP. |
| 2Omy | L + SL | LEAK (L) + S (SL) | ce and pce are in the LEAK state. |
| 3U | E+SE | ETS (E) + S (SE) | ce and pce are in the ETS state. |
| 4Rot | ROX+SE | ROX+S (SE) | ce are in the ROX state, pce are in the ETS state. |
| 5Ama | ROXAma | ROX |
- R 1S .. versus R 1Omy 2U 3S .. and respiration medium
- Intac cells & succinate: ADP is not permeable through the plasma membrane of intact cells, and this is the case for succinate in many cell types (Steinlechner-Maran 1997 Transplantation). If succinate and ADP are accessible to mitochondria in a fraction of permeabilized (non-viable) cells, respiration will be stimulated, if these mitochondria retain a functional S-pathway (CII-linked respiration). Preservation of mitochondrial respiratory function provides the argument of using mitochondrial respiration medium (MiR05) in this CCP with cells of limited viability (Stadlmann_2006_Biochem Biophys).
