SUIT-003 Ce1;ce2SD;ce3Omy;ce4U-: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|abbr= | |abbr=CC_R 1Omy 2U 3Rot 4S 5Ama | ||
|description=[[File:Ce_R 1S 2Omy 3U 4Rot 5Ama.jpg|right|200px]] | |description=[[File:Ce_R 1S 2Omy 3U 4Rot 5Ama.jpg|right|200px]] | ||
'''[[Categories of SUIT protocols |SUIT-catg]]:''' | '''[[Categories of SUIT protocols |SUIT-catg]]:''' CC+S | ||
'''[[Mark names in DatLab]]:''' Stadlmann 2006 Cell Biochem Biophys | '''[[Mark names in DatLab]]:''' Stadlmann 2006 Cell Biochem Biophys | ||
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:::: '''[[SUIT protocol pattern]]:''' complex [[coupling control protocol]] (CCP) | :::: '''[[SUIT protocol pattern]]:''' complex [[coupling control protocol]] (CCP) | ||
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Revision as of 08:58, 29 August 2016
- high-resolution terminology - matching measurements at high-resolution
SUIT-003 Ce1;ce2SD;ce3Omy;ce4U-
Description
SUIT-catg: CC+S
Mark names in DatLab: Stadlmann 2006 Cell Biochem Biophys
DatLab-Excel template: in prep.: Stadlmann 2006 Cell Biochem Biophys.xlsx
Abbreviation: CC_R 1Omy 2U 3Rot 4S 5Ama
Reference: Stadlmann 2006 Cell Biochem Biophys
MitoPedia concepts:
SUIT protocol
- SUIT protocol pattern: complex coupling control protocol (CCP)
| Step | Respiratory state | Pathway control | Comment |
|---|---|---|---|
| R | ROUTINE | ce | pce without substrate are in a state of ROX. |
| 1SD | R&SP | ROUTINE & S (R&P) | pce are in state SP. |
| 2Omy | R&SL | ROUTINE & S (L) | ce and pce are in the LEAK state. |
| 3U | E&SE | ETS & S (E) | ce and pce are in the ETS state. |
| 4Rot | SE | ROX & S (E) | ce are in the ROX state, pce are in the ETS state. |
| 5Ama | ROXAma | ROX |
- R 1S .. versus R 1Omy 2U 3S .. and respiration medium
- Intac cells & succinate: ADP is not permeable through the plasma membrane of intact cells, and this is the case for succinate in many cell types (Steinlechner-Maran 1997 Transplantation). If succinate and ADP are accessible to mitochondria in a fraction of permeabilized (non-viable) cells, respiration will be stimulated, if these mitochondria retain a functional S-pathway (CII-linked respiration). Preservation of mitochondrial respiratory function provides the argument of using mitochondrial respiration medium (MiR05) in this CCP with cells of limited viability (Stadlmann_2006_Biochem Biophys).
