SUIT-003 Ce1;ce2SD;ce3Omy;ce4U-: Difference between revisions
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:::: '''[[SUIT protocol pattern]]:''' complex [[coupling control protocol]] (CCP) | :::: '''[[SUIT protocol pattern]]:''' complex [[coupling control protocol]] (CCP) | ||
'''SUIT states:''' 1-4[[PM]](LPcE) 5[[PGM]] 6[[PGMS]] 7[[OctPGMS]] 8[[S]] 9[[SGp]] 10[[ROX]] 11[[Tm]] 12[[ROX]] | |||
{| class="wikitable" border="1" | |||
|- | |||
! Step | |||
! Respiratory state | |||
! Pathway control | |||
! Comment | |||
|- | |||
| R | |||
| [[ROUTINE]] | |||
| [[Intact cells |ce]] | |||
| [[Permeabilized tissue or cells |pce]] without substrate are in a state of ROX. | |||
|- | |||
| 1SD | |||
| R<small>&</small>S<sub>''P''</sub> | |||
| [[ROUTINE]] & [[S]] (''R''<small>&</small>''P'') | |||
| pce are in state S<sub>''P''</sub>. | |||
|- | |||
| 2Omy | |||
| R<small>&</small>S<sub>''L''</sub> | |||
| [[ROUTINE]] & [[S]] (''L'') | |||
| ce and pce are in the LEAK state. | |||
|- | |||
| 3U | |||
| E<small>&</small>S<sub>''E''</sub> | |||
| [[ETS]] & [[S]] (''E'') | |||
| ce and pce are in the ETS state. | |||
|- | |||
| 4Rot | |||
| S<sub>''E''</sub> | |||
| [[ROX]] & [[S]] (''E'') | |||
| ce are in the ROX state, pce are in the ETS state. | |||
|- | |||
| 5Ama | |||
| ROX<sub>Ama</sub> | |||
| [[ROX]] | |||
| | |||
|} | |||
:::* '''R 1S ..''' versus '''R 1Omy 2U 3S .. and respiration medium''' | :::* '''R 1S ..''' versus '''R 1Omy 2U 3S .. and respiration medium''' | ||
:::: '''Intac cells & succinate''': | :::: '''Intac cells & succinate''': ADP is not permeable through the plasma membrane of intact cells, and this is the case for succinate in many cell types ([[Steinlechner-Maran 1997 Transplantation]]). If succinate and ADP are accessible to mitochondria in a fraction of permeabilized (non-viable) cells, respiration will be stimulated, if these mitochondria retain a functional S-pathway (CII-linked respiration). Preservation of mitochondrial respiratory function provides the argument of using mitochondrial respiration medium (MiR05) in this CCP with cells of limited viability ([[Stadlmann_2006_Biochem Biophys]]). | ||
Revision as of 08:49, 29 August 2016
- high-resolution terminology - matching measurements at high-resolution
SUIT-003 Ce1;ce2SD;ce3Omy;ce4U-
Description
SUIT-catg: CeS
Mark names in DatLab: Stadlmann 2006 Cell Biochem Biophys
DatLab-Excel template: in prep.: Stadlmann 2006 Cell Biochem Biophys.xlsx
Abbreviation: Ce_R 1Omy 2U 3Rot 4S 5Ama
Reference: Stadlmann 2006 Cell Biochem Biophys
MitoPedia concepts:
SUIT protocol
- SUIT protocol pattern: complex coupling control protocol (CCP)
SUIT states: 1-4PM(LPcE) 5PGM 6PGMS 7OctPGMS 8S 9SGp 10ROX 11Tm 12ROX
| Step | Respiratory state | Pathway control | Comment |
|---|---|---|---|
| R | ROUTINE | ce | pce without substrate are in a state of ROX. |
| 1SD | R&SP | ROUTINE & S (R&P) | pce are in state SP. |
| 2Omy | R&SL | ROUTINE & S (L) | ce and pce are in the LEAK state. |
| 3U | E&SE | ETS & S (E) | ce and pce are in the ETS state. |
| 4Rot | SE | ROX & S (E) | ce are in the ROX state, pce are in the ETS state. |
| 5Ama | ROXAma | ROX |
- R 1S .. versus R 1Omy 2U 3S .. and respiration medium
- Intac cells & succinate: ADP is not permeable through the plasma membrane of intact cells, and this is the case for succinate in many cell types (Steinlechner-Maran 1997 Transplantation). If succinate and ADP are accessible to mitochondria in a fraction of permeabilized (non-viable) cells, respiration will be stimulated, if these mitochondria retain a functional S-pathway (CII-linked respiration). Preservation of mitochondrial respiratory function provides the argument of using mitochondrial respiration medium (MiR05) in this CCP with cells of limited viability (Stadlmann_2006_Biochem Biophys).
