SUIT-002 O2 mt D005

high-resolution terminology - matching measurements at high-resolution

SUIT-002 O2 mt D005

Description

Abbreviation: FNSGp(PGM)

Reference: A: SUIT 2 is the RP2 - MiPNet21.06 SUIT RP

SUIT number: 2

MitoPedia concepts: SUIT protocol, SUIT A

MitoPedia: SUIT - SUIT reference protocol RP2

SUIT-category: FNSGp(PGM)

SUIT protocol pattern: diametral

Harmonized SUIT protocols

SUIT RP1 SUIT RP2

SUIT RP1: 1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10AsTm;11Azd

SUIT RP2: 1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd

Harmonization between RP1 and RP2

RP2 can be assessed in cells after the selective plasma membrane permeabilization. Digitonin is a mild detergent that permeabilizes plasma membranes. The optimum effective digitonin concentration for complete plasma membrane permeabilization of cultured cells can be determined directly in a respirometric protocol (see Digitonin). The use of intact cells allows the possibility to evaluate respiration in two different modules: intact cells and permeabilized cells. ROUTINE respiration (ce1) is obtained before digitonin is employed, whereas after the use of digitonin we can address differentet pathway control states.

SUIT RP1 in cells: ce1;1Dig;1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10Tm;11Azd

SUIT RP2 in cells: ce1;1Dig;1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azdd

Harmonization between RP1 and RP2 in cells

SUIT O2 DLP for RP2 (DatLab 7.1)

DL-Protocols (DLP) for substrate-uncoupler-inhibitor titrations (see MitoPedia: SUIT) guide through a sequence of coupling and pathway control states. A specific SUIT protocol can be assigned to O2k-Chamber A or B or both. DL-Protocols for different SUIT protocols can be found in the library of protocols MitoPedia: SUIT. Instrumental DL-protocols are displayed in the DL-Protocol library.

Download instructions.
To start the download click on the  icon. Depending on your browser you will encounter different download scenarios.
(i) A window opens to select whether to "open file with" or to "save file". Please always choose the option "save file"; opening the file directly will not work due to safety reasons.
(ii) When you click the download icon, some browsers will display the text/content of the .DLP file instead of downloading it. In this case go back to this download page and right-click the download icon and choose "save linked file as" from the context-menu. A window will open to save the file. In some cases the suffix of the file might be wrong (e.g. ".HTML") - you must correct the suffix of the file to ".DLP".

DL-Protocol	Description	Download - Protocol .DLP	Download - Analysis .XLSX
RP2pce(Dig)	SUIT reference protocol RP2 for permeabilized cells (pce) with digitonin
RP2mt	SUIT reference protocol RP2 for mt-preparations
RP2pfi	SUIT reference protocol RP2 for permeabilized fibers
RP2pce(Dig)_PBMC_PLT	SUIT reference protocol RP2 for PBMC and PLT

References