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| {{MitoPedia | | {{MitoPedia |
| | |SUIT number=SUIT-2_mt_A05 |
| | |description=[[File:1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot-.png|400px]] |
| |abbr=FNSGp(PGM) | | |abbr=FNSGp(PGM) |
| |SUIT number=2_mt
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| |application=O2 | | |application=O2 |
| |description=[[File:1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot-.png|400px]]
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| |info='''A: SUIT 2 is the RP2''' - [[MiPNet21.06 SUIT RP]] | | |info='''A: SUIT 2 is the RP2''' - [[MiPNet21.06 SUIT RP]] |
| }} | | }} |
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| __TOC__ | | __TOC__ |
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| | == DL-Protocol and template == |
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| == Harmonized SUIT protocols ==
| | :::: [[1D;2OctM;3P;4G;5S;6Gp;7U;8Rot-#DL-Protocols_for_SUIT_protocol_RP2_.28DatLab_7.1.29|SUIT RP2]]:1D;1M.1;2Oct;2c;2M2;3P;4G;5S;6Gp;7U;'''8Rot''';9Ama;'''10Tm''';11Azd |
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| === RP1 and RP2 ===
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| '''SUIT RP1''' [[File:1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10AsTm;11Azd.png|400px|SUIT-RP1]]
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| '''SUIT RP2''' [[File:1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd.png|500px|SUIT-RP2]]
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| :::: [[1PM;2D;3U;4G;5S;6Oct;7Rot;8Gp-|SUIT RP1]]: 1PM;2D;2c;3U;4G;5S;6Oct;7Rot;'''8Gp''';9Ama;'''10AsTm''';11Azd
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| :::: '''SUIT RP2''': 1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;'''10Rot''';11Ama;'''12AsTm''';13Azd
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| [[File:RP1&RP2.png|400px]] '''Harmonization between RP1 and RP2'''
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| === RP1 and RP2 in cells ===
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| RP2 can be assessed in cells after the selective plasma membrane permeabilization. Digitonin is a mild detergent that permeabilizes plasma membranes. The optimum effective digitonin concentration for complete plasma membrane permeabilization of cultured cells can be determined directly in a respirometric protocol (see [[Digitonin]]). The use of intact cells allows the possibility to evaluate respiration in two different modules: intact cells and permeabilized cells. ROUTINE respiration (ce1) is obtained before digitonin is employed, whereas after the use of digitonin we can address differentet [[Electron transfer-pathway state|Electron transfer-pathway statea]].
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| :::: [[1PM;2D;3U;4G;5S;6Oct;7Rot;8Gp-#Harmonized_SUIT_protocols|SUIT RP1 in cells]]: '''ce1''';1Dig;1PM;2D;2c;3U;4G;5S;6Oct;7Rot;'''8Gp''';9Ama;'''10Tm''';11Azd
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| :::: '''SUIT RP2 in cells''': '''ce1''';1Dig;1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;'''10Rot''';11Ama;'''12AsTm''';13Azdd
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| [[File:RP1&RP2_pce(Dig).png|400px]] '''Harmonization between RP1 and RP2 in cells'''
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| === RP1 and RP2 in PBMCs and PTLs ===
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| In PBMCs and PLTs the reference protocol RP1 is performed in the absense of Oct: [[1PM;2D;3U;4G;5S;6Rot;7Gp-|SUIT 1 for PBMCs and PTLs]]
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| :::: [[1PM;2D;3U;4G;5S;6Oct;7Rot;8Gp-#Harmonized_SUIT_protocols|SUIT RP1 in PBMCs and PTLs]]'''SUIT RP1 for PBMCs and PLTs''':1PM;2D;2c;3U;4G;5S;6Rot;'''7Gp''';8Ama;'''9AsTm''';10Azd
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| :::: '''SUIT RP2 PBMCs and PLTs''': 1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;'''10Rot''';11Ama;'''12AsTm''';13Azd
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| [[File:Reference protocol RP1&RP2_pce_PBMCs_PTLs.png|400px]] '''Harmonization between RP1 and RP2 in PBMC and PTL cells'''
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| == SUIT O2 DLP for SUIT 2 (RP2) (DatLab 7.3) == | | == SUIT O2 DLP for SUIT 2 (RP2) (DatLab 7.3) == |
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| ''Updating this page (2018-06-13) for DatLab 7.3 DLP files'' | | :::: ''Updating this page (2018-06-13) for DatLab 7.3 DLP files'' |
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